• Korean Journal of Plant Tissue Culture
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• v.27 no.4
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• pp.339-343
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• 2000

When plants are exposed to subfreezing temperatures ice crystals are forming within extracelluar space in leaves. The growth of ice crystal is closely related to the degree of freezing injury. It was shown that an antifreeze protein binds to an ice nucleator through hydrogen bonds to prevent growth of ice crystal and also reduces freezing damage. The antifreeze proteins in plants are similar to PR proteins but only the PR proteins induced upon cold acclimation were shown to have dual functions in antifreezing as well as antifungal activities. Three of the genes encoded for CLP, GLP, and TLP were isolated from barley and Kentucky bluegrass based on amino acid sequence revealed after purification and low temperature-inducibility as shown in analysis of the protein. The deduced amino acid of the genes cloned showed a signal for secretion into extracellular space where the antifreezing activity sup-posed to work. The western analysis using the antisera raised against the antifreeze proteins showed a positive correlation between the amount of the protein and the level of freeze tolerance among different cultivars of barely. Besides it was revealed that TLP is responsible for a freeze tolerance induced by a treatment of trinexapac ethyl in Kentucky bluegrass. Analysis of an overwintering wild rice, Oryza rufipogon also showed that an acquisition of freeze tolerance relied on accumulation of the protein similar to CLP. The more direct evidence for the role of CLP in freeze tolerance was made with the analysis of the transgenic tobacco showing extracellular accumulation of CLP and enhanced freeze tolerance measured by amount of ion leakage and rate of photosynthetic electron transport upon freezing. These antifreeze proteins genes will be good candidates for transformation into crops such as lettuce and strawberry to develop into the new crops capable of freeze-storage and such as rose and grape to enhance a freeze tolerance for a safe survival during winter.

• Journal of the Korean Society of Physical Medicine
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• v.3 no.1
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• pp.1-9
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• 2008

Purpose : The purpose of this study is to assess the effects of ice immersion to the ankle, foot on vertical jump, isokinetic exercise. Methods : Thirty volunteers from universal students who had not sustained an injury to the lower extremity within the past 6 months were randomly assigned to either an experimental or control group. Subjects in the experimental group performed vertical jump and isokinetic exercise before and after the application of a 15-minute ice immersion($50-55^{\circ}F$) to the ankle and foot. Subjects in the control group performed vertical jump and isokinetic exercise before and after the application of a 15-minute resting. Results : Shuttle run was not significantly decreased after than before the application of a 15-minute ice immersion($50-55^{\circ}F$) to the ankle and foot(p<0.05). Vertical jump was significantly decreased after than before the application of a 15-minute ice immersion($50-55^{\circ}F$) to the ankle and foot(p<0.05). Speed $60^{\circ}$ of peak torque was significantly decreased after than before the application of a 15-minute ice immersion($50-55^{\circ}F$) to the ankle and foot(p<0.05). Speed $120^{\circ}$ of peak torque was significantly decreased after than before the application of a 15-minute ice immersion($50-55^{\circ}F$) to the ankle and foot(p<0.05). Conclusion : we think because the ice immersion decrease active, physical therapist should carefully consider the consequence of cold therapy to increase active.

• Journal of Sericultural and Entomological Science
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• v.25 no.1
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• pp.21-26
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• 1983

The mulberry variety, Jeonweon No. 1, has been cultivated in some parts of Ho-Nam District since the variety was selected at the Jeonbuk Provincial Silkworm Egg Production Station in the latter half of 1920s. However, the characteristics of the ariety have not been reported. Therefore, the cultivation experiment on the variety was carried out to investigate the characteristics of the variety at Jeonju and Suweon for four years from 1979 to 1982. 1. The mulberry variety, Jeonweon No. 1, belongs to the Morus alba L. and the sprouting stage is medium. 2. The branch growth of the variety was rather slow, however the number of branches was more and the posture of branches opened wide. 3. The rate of cold injury of shoot tips was a little high, but resistance to disease and the lodging of branches was weaker, compared with the Gaeryangbbong variety. 4. The annual yield of leaves of the variety per 10a was 1,474kg, 14% less than that of the Gaeryangbbong variety.

• Herbal Formula Science
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• v.28 no.4
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• pp.365-384
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• 2020

Objectives : This report describes 241 prescriptions related to the use of Gardenia Fructus blended prescriptions from Dongeuibogam. Methods : The following conclusions were reached through investigations on the prescriptions that use Gardeniae Fructus. Results : The Gardeniae Fructus is used in 49 fields of treatment such as urinary diseases, jaundice and furuncle, etc. Prescriptions that utilize Gardeniae Fructus are used in the treatment of fever, eye disease, hemorrhage, skin disease, ear disease, internal disease and so on. In the view of the causative agent of a disease, the prescriptions which are compounded with Gardeniae Fructus are related to endogenous agents such as jaundice, seven emotion, alcohol, exogenous agents such as cold, wind, heat and non-endo-exopathogenic factors like disease due to external factor, injury. The dosage of Gardeniae Fructus is 1pun(about 0.375) to 5don 5pun(about 21g), however 5pun(about 1.87g)~1don(about 3.75g) has been taken the most for clinical application. Conclusions : Hwangryunhaedok-tang is the most base prescriptions which use the Gardenia Fructus. Gardeniae Fructus is mainly used as a raw material.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.227-234
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• 2014

The responses to chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previously determined cold tolerant NC76 and 'Chipper'), and Chinese and Japanese germplasms. Seedlings of each entry were exposed to $4^{\circ}C$ (Experiment 1) and $1^{\circ}C$ (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under 80% relative humidity (RH) and $149{\mu}moles{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux (PPF). The chilling response [damage rating (DR)] of each accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate, 4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of chilling tolerant line NC76 and 'Chipper' after chilling w as 1 and 1.1, respectively. Korean 'Nacdongchungjang' was most sensitive to chilling temperatures [DR = 2.3] when compared to the other entries examined. The sensitivity to chilling of 'Nacdongchungjang' was followed by Chinese 'Dongguan' [DR = 1.7]. In contrast, 'Saeronchungjang' (DR = 1) and 'Janghyungnachap' (DR = 1) were the most chilling tolerant of the Korean accessions examined and equivalent to the response of line NC76 and 'Chipper'. Nevertheless, chloroplast type genotyping of these accessions with known chilling-linked sdCAPS genomic markers revealed genotypic differences between chilling tolerant lines (NC76 and 'Chipper') and all Korean lines examined.

• Child Health Nursing Research
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• v.16 no.4
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• pp.268-276
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• 2010

Purpose: The purpose of this study was to provide basic data to improve coping abilities of elementary school students in emergency situations. Method: The data were collected from 1633 3-6th grade elementary school students using a self-report questionnaire. Results: 1) The mean score for the coping ability of the students was low at .38(${\pm}.36$). 2) The mean scores for the 8 categories were, [Care for common symptoms], .54(${\pm}.48$), [Call for help & check], .47(${\pm}.43$), [Care for injury by heat cold & foreign body], .39(${\pm}.45$), [Care for tissue damage], .36(${\pm}.44$), [Offering help], .36(${\pm}.42$), [Rescue & escape], .31(${\pm}.51$), [Evacuation], .28(${\pm}.49$), and [Appropriate response for the situation], .27(${\pm}.43$). The mean scores for 2 areas were, 'Acting tips', .35(${\pm}.36$) and 'First aid', .42(${\pm}.40$). There were significant differences in coping ability according to students' gender (t=4.964, p<.001), and family type (t=-2.484, p=.013). 4) Among the categories, there were significant correlations between all 8 categories and significant correlation between 'Acting tips' and 'First aid' (r=.808, p<.001), 'Acting tips' and 'Coping ability' (r=.956, p<.001), and 'First aid' and 'Coping ability' (r=.946, p<.001). Conclusion: The findings indicate a need for programs to improve the coping ability of elementary school students in emergency situations.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.5
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• pp.1078-1105
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• 2008

An empirical formula that has been empirically shown to be effective for a particular condition can be said to add medicinal materials to an existing formula or to make a new prescription by the Korean medical doctors' empirical clinic. This dissertation aims to study the prescriptions according to A Confidential Document of A Distinguished Medical Family written by the writer's ancestor and to contribute to the development of Korean medicine. A Confidential Document of A Distinguished Medical Family is a medicinal recipe that is an heirloom of the Gyeongju Kim family for the eighth generation. And the date and the writer of the book are unknown. The contents of the book consist of 18 parts and are made up of about 300 medical recipes. Of these, 158 formulas and indications of cold damage, wind, urination, stool, jaundice, and internal injury are found in the book. From among these, 61 formulas was directly quoted out of The Precious Mirror of Oriental Medicine, 97 formulas are adjusted in proportion or combined. By the parity of reasoning of the above, I think that this book was published after The Precious Mirror of Oriental Medicine. I will continue to study other formulas except the formulas above mentioned.

• Journal of Preventive Medicine and Public Health
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• v.22 no.1 s.25
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• pp.102-115
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• 1989

The purpose of this study was to find out morbidity rate and utilization pattern of medical services of sailors on a voyage. The subjects of this study were 470 sailors who are sailors' trainee and safety-trainee for embarkation in a ship. Data were collected by means of personal survey conducted from September 5 to October 30, 1988. The subjects were sailed 12.6 months on the average. During the voyage, 49.4% of the sailors had sickness more than once, and 24.7% was unable to work more than a day because of illness. The average monthly morbidity rate was 85.7 per 1,000 and the monthly prevalence rate of disease that was unable to work more than a day was 21.2 per 1,000. The sailors of 40-49 years old had the highest morbidity rate of 103.6 per 1,000/month. The number of days activity restricted due to sickness was 93.4 days per 1,000 persons, and the average duration of illness was 11.2 days per sickness. The proportion of treatment days to sickness days was 48.8%. Considering morbidity unable to work more than a day, the number of those who had traumatic injury were 17.2%, which revealed the highest rate, influenza or cold were 15.5%, and the digestive disease 11.2%. Less than half(42.1%) of patients were treated with medicine kept in the cabin. However, 2.1% was transferred by helicopter and 29.5% was treated at hospitals or clinics in nearby port. In the cabin, 67.4% of patients was cured completely.

• Archives of Plastic Surgery
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• v.36 no.2
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• pp.153-160
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• 2009

Purpose: The heterodigital or homodigital artery island flap is a popular method of reconstruction for finger defects. Sometimes, digital artery island flap has some disadvantages such as postoperative flap edema, congestion, and partial necrosis of the flap margin. However, we could decrease these disadvantages by means of venous superdrainage. The aim of this study is to report usefulness and postoperative results of venous supercharging digital artery island flaps for finger reconstruction. Methods: From March of 2005 to March of 2008, a total of eight patients with soft tissue defects of the finger underwent venous supercharging digital island flap transfer. Briefly, the flap is harvested along with dorsal vein that is then anastomosed to a recipient vein in an end - to - end fashion, after flap transfer and insetting. Using this technique, eight patients were operated on, ranging in age 23 to 52 years. Results: All the flaps survived with a success rate of 100 percent, thus fully satisfying the reconstructive requirements. No postoperative flap congestion was recognized, obviating the need to take any measures for venous engorgement, such as suture removal. Among 8 cases, it was possible to make an long - term and follow - up observation more than 6 months. In these cases, the fact that light touches and temperature sensations can be detected in all the flaps. Cold intolerance and hyperesthesia were not seen in our series. Conclusion: Providing good harmony with conventional methods and microsurgery, inclusion of a vein with the heterodigital and homodigital artery island flap allows a more reliable and safer reconstructive choice for finger defects. The venous supercharged island flap is a reliable flap with a consistent arterial structure, and with its augmented venous drainage, it is more reliable, providing single - stage reconstruction of adjacent finger defects, including the fingertip.

• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.4
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• pp.663-668
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• 2011

Galgeun-tang (GGT) has been a great source for treating cold diseases in the folk medicine recipe. Carbon tetrachloride ($CCl_4$) is one type of hepatotoxin that can eventually cause liver injury. During the experiment, we first studied the protective effects of GGT against $CC_4$-induced hepatotoxicity. GGT was pretreated for 3 h, and 1% $CCl_4$ was added to mouse primary liver cells. After 4 h, ROS generation and expression of antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx)) were analyzed by FACS and real time PCR. Also, the activities of ALT and LDH were measured using cultured medium. The hepatic levels of TNF-alpha and iNOS, which are related to inflammation and stress response gene, HSP72 and HO-1 were analyzed by PCR or real time PCR. Liver tissues were analyzed by HE stain. From the observation, we discovered that GGT treatment protects $CCl_4$-induced hepatotoxicity, and that GGT pretreatment decreases ROS generation, TNF-alpha and iNOS expression. However, gene expression of CAT, SOD, GPx, HSP72 and HO-1 were increased by GGT. These results lead to the conclusion that GGT has protective effects against $CCl_4$-induced hepatotoxicity.