• Journal of the korean veterinary medical association
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• v.43 no.7
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• pp.609-620
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• 2007

• Journal of Veterinary Clinics
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• v.20 no.2
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• pp.166-171
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• 2003

Milk samples were collected from a total of 418 quarters of 214 Holstein cows. Of these, samples which were positive on California Mastitis Test (CMT) and above 200,000 cells/ml by somatic cell counts were subjected to bacteriological examination and antimicrobial susceptibility test. Major pathogens responsible for mastitis included coagulase-negative staphylococci (34.3%), coagulase-positive staphylococci (21.5%), gran-negative rod (coliforms and noncoliforms: 12.6%) and Streptococcus spp. (8.4%). These strains were tested with 13 antimicrobial agents by the Kirby and Bauer standardized disc diffusion method. The isolated pathogens were mostly susceptible to amoxicillin and cephalothin, but were resistant to erythromycin.

• Korean Journal of Veterinary Research
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• v.24 no.2
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• pp.163-168
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• 1984

The in vitro activity of penicillins(penicillin G, ampicillin, carbenicillin, methicillin and cloxacillin) and gentamicin to Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitic milk samples was determined. The growth of all isolates of staphylococci tested was inhibited by cloxacillin and methicillin at a concentration of $0.78{\mu}g/ml$ and by gentamicin at a minimum inhibitory concentration (MIC) of $1.56{\mu}g/ml$. Of the 140 strains of Staphylococcus aureus isolated 99(70.7%) gave positive reactions for penicillinase on the starch-iodine test. Of 121 isolates of coagulase-negative staphylococci, 58(47.9%) showed penicillinase production, but all the Staph. xylosus lacked the ability to produce penicillinase. MIC of penicillin G of the penicillinase-positive strains was $0.2{\mu}g/ml$ or more. The combination of cloxacillin with gentamicin showed a synergistic effect by inhibiting regrowth of the tested organism.

• Korean Journal of Veterinary Research
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• v.22 no.1
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• pp.15-21
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• 1982

The biochemical properties of strains of Staphylococcus aureus isolated from cattle with peracute gangrenous mastitis, acute systemic mastitis and chronic mastitis were examined. Of 261 strains of Staphylococci isolated from quarters with clinical mastitis, 140 (53.6%) were classified as Staphylococcus aureus and 121 (46.4%) were coagulase-negative staphylococci. All the strains of Staphylococcus aureus isolated from quarters with peracute gangrenous mastitis and acute systemic mastitis showed production of alpha heamolysin, coagulase, lipase, phosphatase, nuclease and gelatinase, and fermentation of mannitol. However, of 114 strains of Staphylococcus aureus obtained from quarters with chronic mastitis, 64 (56.1%) possessed alpha lysin, and 43 (37.7%) produced lipase in egg yolk medium. The most common hemolytic pattern of the strains associated with chronic mastitis was ${\beta}{\delta}$ type.

• The Journal of the Korean dental association
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• v.16 no.2 s.105
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• pp.143-146
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• 1978

The bacterial behaviour against antibiotics and pattern were examined with 88 strains of 10 species, isolated from pus collected in the infirmary of college of dentistry, and Seoul national university hospital during recent 7 years. 1. Isolated bacterial species and their relative incidences were, as follows, coagulase positive staphylococcus 50%, alpha hemolytic streptococcus 17%, Pseudomonas aeruginosa 10% and coagulase negative staphylococcus 8%. 2. Gentamycin reveals the highest sensitibity and cephalosporine follows it.

• Proceedings of the Korean Society of Veterinary Service Conference
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• 2000.06a
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• pp.180-181
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• 2000

• Food Science of Animal Resources
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• v.37 no.2
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• pp.264-273
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• 2017

The aim of this study was to investigate the effects of production systems and milk collection periods on the somatic cell count (SCC), some microbiological properties, total aerobic mesophilic bacteria (TAMB), coliform, Staphylococcus aureus (S. aureus), yeast and mould) and antibiotic residue of milk; in Turkey. Milk samples were collected from 9 conventional farms and 9 organic farms during one year time, at six different months (December 2013 to October 2014), and all farms were selected from the same geographical locations. All organically managed farms had organic production certificates given by the Republic of Turkey Ministry of Food, Agriculture and Livestock. The count of TAMB, coliform, and coagulase positive S. aureus were affected by production systems at the level of p<0.01; yeast and mold, and somatic cell count (SCC) were affected at the level of p<0.05. But, differences according to months were statistically significant only on TAMB (p<0.01) and coliform (p<0.05) counts. The general means of TAMB, coliform and yeast and mould counts of the organic milk (OM) were significantly lower (p<0.05), while the general means of SCC and coagulase positive S. aureus count of the OM was significantly higher (p<0.05) compared to conventional milk (CM). Antibiotic residue was determined in one of the CM sample and in two of the OM samples. Our study is the first research that compared conventional and organic milk in Turkey. This study indicated that the microbiological quality of OM was the higher in terms of TAMB, coliform and yeast and mould, whereas was the lower in relation to SCC and coagulase positive S. aureus counts. But, the quality of both milk types should be improved.

• Microbiology and Biotechnology Letters
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• v.41 no.4
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• pp.462-468
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• 2013

Jeotgal is a generic term given to the high-salt-fermented seafood of Korea. This study aimed at developing an overview of the bacterial community present in Ojingeo-jeotgal, a highly consumed type of jeotgal, which is made with squid. Bacteria were isolated and purified from two samples on six different kinds of media and identified by 16S rRNA gene sequence analysis. Among the 121 total isolates, the most dominant genus was Bacillus, followed by coagulase-negative staphylococci (CNS) and lactic acid bacteria (LAB). CNS were detected in both samples, but LAB were observed in only a single sample. Six strains of Bacillus species inhibiting the growth of food pathogens, Staphylococcus aureus and Vibrio parahaemolyticus, were selected from the 121 isolates. These were found to inhibit the growth of both pathogens in addition to displaying proteolytic activities on media containing 6% NaCl and 2% skim milk.

• Korean Journal of Veterinary Service
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• v.23 no.1
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• pp.61-69
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• 2000

Thirty-one dairy cow from two farm(more than 500,000 cells/ml of bulk milk) in Kyongbuk northern province were selected because of their high somatic cell(more than 500,000 cells/ml of milk In individual cow). Each cow received. staphylococcus aureus vaccine(Labac Staph) and immunostimulant(Ultracon) by intramuscular injection to be repeated every fifteen days for S times. The present study was investigated variation of somatic cell after administration of Labac Staph and Ultracon, and antibiotics resistance of isolated staphylococcus spp from milk in selected cow. The results obtained through the survey were summarized as follows ; 1. Ten dairy cow was injected in A farm. Chronic mastitic two cow after 2rd injection was weeded out the herd. Decrease rate of somatic cell after 1st, 2nd, ,3rd, 4th and 5th administration were 41.4%, 35.6%, 56.4%, 65.4% and 36.7%, respectively. Twenty-one cow was injected in B farm. Chronic mastitic five cow after ,3rd injection was weeded out the herd. Decrease rate of somatic cell after 1st, 2nd, 3rd, 4th and 5th administration were 36.9%, 59.9%, 24.5%, 62.6% and 78.4%, respectively. 2. In A farm, isolated staphylococcus spp were identified as S hyicus 2 strains(11.8%), coagulase negative staphylococcus 15 stains(89.2%) and S epidermidis 6strain(35.3%). In B farm, isolated staphylococcus spp were identified as S aureus 19 strains(55.98%) and coagulase negative staphylococcus 15 strains (44.2%). 3. In A fm, antibiotics resistant rate of isolated staphylococcus spp was high at ampicillin, penicillin and kanamycin, and middle at neomycin, streptomycin and erythromycin. in B farm, antibiotics resistant rate was moderate at ampicillin, penicillin, gentamicin, ka-namycin, neomycin, streptomycin, erythromycin and tetracycline, and coagulase negative staphylococcus spp was moderate at streptomycin.

• Korean Journal of Veterinary Research
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• v.58 no.1
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• pp.51-55
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• 2018

Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.