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http://dx.doi.org/10.14405/kjvr.2018.58.1.51

Molecular identification of coagulase-negative staphylococci by rpoB sequence typing  

Seong, Won-Jin (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University)
Kim, Danil (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University)
Kim, Eun-Kyung (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University)
Ko, Dae-Sung (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University)
Ro, Younghye (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University)
Kim, Jae-Hong (Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University)
Kwon, Hyuk-Joon (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University)
Publication Information
Korean Journal of Veterinary Research / v.58, no.1, 2018 , pp. 51-55 More about this Journal
Abstract
Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.
Keywords
16S rRNA gene sequencing; bovine mastitis; coagulase-negative staphylococci; rpoB sequence typing;
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