Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Molecular identification of coagulase-negative staphylococci by rpoB sequence typing

rpoB 염기서열 분석을 이용한 응고효소 음성 포도알세균 분자 동정

  • Seong, Won-Jin (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Danil (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Eun-Kyung (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Ko, Dae-Sung (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Ro, Younghye (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Jae-Hong (Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Kwon, Hyuk-Joon (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University)
  • 성원진 (서울대학교 수의과대학 산업동물의학연구실) ;
  • 김단일 (서울대학교 수의과대학 산업동물의학연구실) ;
  • 김은경 (서울대학교 수의과대학 산업동물의학연구실) ;
  • 고대성 (서울대학교 수의과대학 산업동물의학연구실) ;
  • 노영혜 (서울대학교 수의과대학 산업동물의학연구실) ;
  • 김재홍 (서울대학교 수의과대학 조류질병학연구실) ;
  • 권혁준 (서울대학교 수의과대학 산업동물의학연구실)
  • Received : 2017.12.07
  • Accepted : 2018.03.08
  • Published : 2018.03.31
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Abstract

Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.

Keywords

References

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