• Title/Summary/Keyword: co-workers

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Studies on Enzymatic Characteristic′s of Adenylate Kinase from Baker′s Yeast (제빵효모 Adenylate Kinase의 효소학적 특성에 관하여)

  • ;Takahisa Ohta;Hiroshi Sakai
    • Microbiology and Biotechnology Letters
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    • v.12 no.4
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    • pp.277-283
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    • 1984
  • In the forward reaction (ADP formation) of the adenylate kinase from baker's yeast, dissociation constants from binary complexes are higher by a factor of about 4 times then those from at ternary complexes. In the reverse reaction, dissociation constants from the binary complexes are 2 times higher then those from the ternary complexes. The enzyme showed activities against various nucleotide triphospate in following orders; ATP 100, UTP 18, ITP 9 and GTP 5, of the necleotide monophosphate. only dAMP showed 33% activity of that AMP as phosphate acceptor. Divalent cations were required in enzyme reaction in following orders; $Mg^{2+}$ 100, Co$^{2+}$ 57, Mn$^{2+}$ 54, $Ca^{2+}$ 51, Ni$^{2+}$ 10 and Sn$^{2+}$ 6. AMP, as a substrate inhibitor, competitively inhibited the adenylate kinase at pH 7.2 or 8.0. Inhibition constants of the enzyme showed greater dependence on the pH of the reaction mixture, which was the lower Ki values under higher pH. Adenosine pentaphospho adenosine was competive inhibitor to the enzyme against all substrate, and it showed the same Ki values, 2.9mM. Further, PEP was competive inhibitor with respect to AMP and non-competive inhibitor with respect to MgATP. Adenylate kinase from bakers yeast was similar to mitochondrial type of animal in the contents of aianine, leucine and asparagine or asparatic acid differing from muscle type enzyme. Based on the results and observation, characteristic of yeast adenylate kinase resembled the adenylate kinase of mitochondrial type from animals. Further, difference of characteristics in adenylate kinasa depending upon the workers might be due to the difference of strain used.

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Serum Angiotensin-Converting Enzyme Levels in Coal Worker's Pneumoconiosis (탄광부 진폐증환자의 Angiotensin-Converting Enzyme 활성치)

  • Kim, Kyung-Dong;Choi, Myung-Sook;Lee, Chae-Hoon;Kim, Chung-Sook;Bae, Eun-Kyung
    • Journal of Yeungnam Medical Science
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    • v.6 no.1
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    • pp.109-119
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    • 1989
  • We measured fasting serum angiotensin -converting enzyme (SACE) in 100 healthy controls and 75 coal worker's pneumoconiosis (CWP) patients by a commercial kits($ACEcolor^{(R)}$, Fujirio Inc., Japan) and evaluated this manual method. The linear range extends to an activity of 80U/L. Precision on a commercial control serum (ACE control-$N^{(R)}$, Sigma Co.)with a mean value, of 9.47U/L yielded a within-run and between-run CVs are 5.6% (N=15) and 6.9% (N=14) respectively. SAVE in 75 CWP was $20.3{\pm}5.7U/L$ ($mean{\pm}s.d.$) ; higher than in healthy controls ($13.4{\pm}3.9U/L$, P<0.01). No correlation was found between SACE, sex, and age. The results suggest that the measurement of SACE and follow-up SACE in coal workers may be a useful diagnostic fools for CWP.

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Abdomino-perineal Organ Injuries Caused by Cultivators (경운기 사고에 의해 발생한 복부 및 회음부 장기 손상)

  • Yeo, Kwang Hee;Park, Chan Yong;Kim, Ho Hyun;Park, Soon Chang;Yeom, Seok Ran
    • Journal of Trauma and Injury
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    • v.28 no.2
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    • pp.60-66
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    • 2015
  • Purpose: Cultivator accidents are frequent and often lead to abdomino-perineal organ injury and, if severe, to death. This study presents the clinical characteristics, outcomes, and factors associated with mortality in patients who sustained an abdomino-perineal organ injury in cultivator accidents. Methods: We retrospectively analyzed the records of 53 patients who visited the emergency department of a tertiary hospital with abdomino-perineal organ injuries caused in cultivator accidents from April 2005 to March 2010. Results: All 53 patients had visited other medical institutions before visiting our hospital. Their mean age was $64.0{\pm}11.1$ (range, 20-80) years and 32 (60.4%) patients were 65 or older. The male-to-female ratio was 46:7. The chief complaint was abdominal pain (38 cases, 71.7%). The 53 patients included 41 cultivator operators (77.4%), 11 passengers (20.8%), and 1 passerby (1.9%). The causes of the injuries included a direct impact of the handlebar in 20 cases (37.7%), a rollover in 21 cases (39.6%), a fall in 10 cases (18.9%), and a wheel in two cases (3.8%). Several of the 53 patients had injuries to multiple abdomino-perineal organs, and the injured organs included the liver (23 cases, 26.4%), spleen (16 cases, 18.4%), pancreas (7 cases, 8.0%), small bowel (7 cases, 8.0%), mesentery (6 cases, 6.9%), adrenal gland (5 cases, 5.8%), and other organs. According to the abbreviated injury scale (AIS) dictionary, a thoracic injury was the most frequent co-injury (33 of 53 cases, 62.3%). Abdomino-perineal surgery was performed in 31 cases (58.8%) and angio-embolization was performed for six liver and two kidney injuries. Thirteen patients died (24.5%); all were males. The Injury Severity Scale (ISS) was lower in the survivors ($17.8{\pm}8.5$ vs. $27.0{\pm}16.0$; p=0.010). Conclusion: With the aging of agricultural workers, safety education programs should be implemented. Furthermore, the patient transfer system in agricultural areas must be improved.

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The Effect of Self-status Factors on Self-Efficiency and Job Satisfaction of Fire Service Officials (소방공무원의 자아상태 요인이 자아효능감과 직무만족도에 미치는 영향)

  • Kim, Yeob-Rae;Lee, Hae-Young
    • Fire Science and Engineering
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    • v.33 no.2
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    • pp.145-154
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    • 2019
  • The purpose of this study is to analyze the self-status of fire service personnel to identify their effect on self-efficacy and job satisfaction, and to improve the performance of their tasks in the field and perform their tasks efficiently. A survey of 177 current firefighters in the southern city of Gyeonggi Province conducted a correlation analysis and regression analysis through SPSS 22.0. The first study found that in relation to the self-status factor and the self-efficacy factor of firefighting services, the self-efficacy is rather high and the self-regulating effect is very high. Second, the effects of the self-status factor on the self-efficacy were significant in order of type Adult (A), type Free Child (FC), and type Adapted Child (AC). Third, analysis of correlation between self-status factors and job satisfaction factors showed inadequate correlation in all job satisfaction factors for Critical Parent (CP) types, and static correlation in all job satisfaction for Nurturing Parent (NP) types. Type Adapted Child (AC) was less satisfying to itself, to itself, to pay, to promote, to co-workers. Thus, the current strategic approach based on rationality needs to be strengthened by lowering the authority of the Critical Parent (CP) function in terms of self-status factors and enhancing the Adapted Child (AC) function in the lower satisfaction level of the parent.

A Study on the Emission of VOCs in Parking Lot Floor Coating (주차장 바닥 코팅제의 휘발성유기화합물 배출에 관한 연구)

  • Lee, Seung-Chan;Yoon, Gil-Ho;Park, Yong-Soon;Kil, Bae-Su;Yoon, Hyun-Do
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.23 no.4
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    • pp.152-158
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    • 2019
  • Measure the type and content of VOCs for A-company epoxy coating and B-company floor coating(Type A, B), which are used as flooring materials for parking lots. Than the VOCs used gas detectors to measure gas emissions, assuming the worst environment to reduce errors in external environments in the formaldehyde, toluene and xylene harmful to workers and tenants. As a result, A-company epoxy coating has the largest amount of VOCs, and compared to A-company epoxy coating, B-company floor coating of A type represented about 79% less and B type about 96% less. In addition, A-company epoxy coating was also the highest in gas emission measurement for formaldehyde, toluene and xylene using gas detector after 1 hour and 8 hours in closed environment conditions. B-company floor coating A type was less than A-company epoxy coating, which was about 42.3% less measured. And type B satisfied all TWA even in closed environment conditions.

Development of big data based Skin Care Information System SCIS for skin condition diagnosis and management

  • Kim, Hyung-Hoon;Cho, Jeong-Ran
    • Journal of the Korea Society of Computer and Information
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    • v.27 no.3
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    • pp.137-147
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    • 2022
  • Diagnosis and management of skin condition is a very basic and important function in performing its role for workers in the beauty industry and cosmetics industry. For accurate skin condition diagnosis and management, it is necessary to understand the skin condition and needs of customers. In this paper, we developed SCIS, a big data-based skin care information system that supports skin condition diagnosis and management using social media big data for skin condition diagnosis and management. By using the developed system, it is possible to analyze and extract core information for skin condition diagnosis and management based on text information. The skin care information system SCIS developed in this paper consists of big data collection stage, text preprocessing stage, image preprocessing stage, and text word analysis stage. SCIS collected big data necessary for skin diagnosis and management, and extracted key words and topics from text information through simple frequency analysis, relative frequency analysis, co-occurrence analysis, and correlation analysis of key words. In addition, by analyzing the extracted key words and information and performing various visualization processes such as scatter plot, NetworkX, t-SNE, and clustering, it can be used efficiently in diagnosing and managing skin conditions.

Economic Analysis on the Automation System of the Cultivation Process in the Plant Factory (식물공장 재배 공정 자동화 시스템의 경제성 분석)

  • Jung, Mincheol;Kim, Handon;Kim, Jimin;Choi, Jeongmin;Jang, Hyounseung;Jo, Soun
    • Korean Journal of Construction Engineering and Management
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    • v.23 no.4
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    • pp.57-64
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    • 2022
  • A plant factory is a facility that creates an artificial environment in a controlled space and produces plants systematically through automated facilities. However, automation in the cultivation process is insufficient compared to the internal environment control technology in plant factories. This causes the problem of an increase in operating costs due to the input of a large number of workers. Therefore, this study aims to evaluate economic feasibility by comparing before and after introducing automation in the cultivation process of plant factories. The target plant factory to be analyzed was selected, and the break-even point analysis method was used by comparing the cost required compared to the operating period. As a result, the break-even point was analyzed to be 3.4 years when automation was introduced into six processes for plant cultivation. Therefore, it can be judged that the introduction of automation is excellent in terms of economic feasibility when the target plant factory has been operated for more than 3.4 years. This study is expected to be used as basic data to analyze the economic feasibility of introducing automation in domestic and foreign plant factories.

Evaluation of the Input Status of Exposure-related Information of Working Environment Monitoring Database and Special Health Examination Database for the Construction of a National Exposure Surveillance System (국가노출감시체계 구축을 위한 작업환경측정과 특수건강진단 자료의 노출 정보 입력 실태 평가)

  • Choi, Sangjun;Koh, Dong-Hee;Park, Ju-Hyun;Park, Donguk;Kim, Hwan-Cheol;Lim, Dae Sung;Sung, Yeji;Ko, Kyoung Yoon;Lim, Ji Seon;Seo, Hoekyeong
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.32 no.3
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    • pp.231-241
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    • 2022
  • Objectives: The purpose of this study is to evaluate the input status of exposure-related information in the working environment monitoring database (WEMD) and special health examination database (SHED) for the construction of a national exposure surveillance system. Methods: The industrial and process code input status of WEMD and SHED for 21 carcinogens from 2014 to 2016 was compared. Data from workers who performed both work environment monitoring and special health examinations in 2019 and 2020 were extracted and the actual status of input of industrial and process codes was analyzed. We also investigated the cause of input errors through a focus group interview with 12 data input specialists. Results: As a result of analyzing WMED and SHED for 21 carcinogens, the five-digit industrial code matching rate was low at 53.5% and the process code matching rate was 19% or less. Among the data that simultaneously conducted work environment monitoring and special health examination in 2019 and 2020, the process code matching rate was very low at 18.1% and 5.2%, respectively. The main causes of exposure-related data input errors were the difference between the WEMD and SHED process code input systems from 2020, the number of standard process and job codes being too large, and the inefficiency of the standard code search system. Conclusions: In order to use WEMD and SHED as a national surveillance system, it is necessary to simplify the number of standard code input codes and improve the search system efficiency.

Comparison and Improvement of Domestic and Foreign Regulations for the Prevention of Suffocation Accidents (국외 질식재해 예방규정 비교를 통한 국내 규정 개선방안)

  • Lim, Dae Sung;Lee, Seung kil;Kim, Chi-Nyon;Cho, Kee Hong;Yi, Gwang Yong
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.31 no.1
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    • pp.83-93
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    • 2021
  • Objectives: 'Confined space' was only defined in the Safety and Health Regulations as a place where oxygen deficiency and hydrogen sulfide must be dealt with at the time of the initial enactment (1982). The danger of fire and explosion were added in 2003. We will compare and review the regulations related to confined space work under the current safety and health rules alongside regulations in other countries and prepare a plan to improve the system through enhanced clarity and execution. Methods: In a comparison of systems for the prevention of suffocation in confined spaces in major countries (Germany, United States, Japan) different concepts of the definition of confined spaces in different countries apparently due to differences in each country's legal implementation system, accident analysis methods, the status of safety and health implementation in workplaces, the precautions against actual confined space work, and the definition of confined spaces were found to be not much different between Korea and the other foreign countries. Results: In the case of Germany and the United States, a confined space is defined as a contextual concept rather than a place, so more careful attention is needed from operators or enclosed space managers as it is often necessary to judge the actual workplace. In the case of Korea and Japan, the interior of the place is mainly defined as a place, especially in the case of Japan, which concentrates on oxygen deficiency and hydrogen sulfide poisoning. Conclusions: For measures to improve regulations on the prevention of suffocation accidents in Korea, I would like to propose three major measures to improve the system in the rules on domestic industrial safety and health standards. It is necessary to prepare and provide a guide to ensure that the 18 types of confined spaces currently defined as confined spaces are clearly understood by field management supervisors or workers.

Quality of Dry Cement Mortar for Floor Heating Depending on Water-to-Dry Mortar Rutio (난방을 위한 바닥용 건조 시멘트 모르타르의 혼합수량비 변화에 따른 품질 특성)

  • Park, Sang-Jun;Hwang, Yin-Seong;Lee, Gun-Cheol;Kim, Jong
    • Journal of the Korea Institute of Building Construction
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    • v.21 no.3
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    • pp.181-188
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    • 2021
  • In this study, the various performance of dry cement mortar for Korean floor heating system depending on water-to-dry mortar ratios (W/DM) applied in project site was evaluated. According to the experiment conducted, the importance of mixing water for dry cement mortar was revealed by resultant performance or quality of the dry cement mortar for floor finishing by changing W/DM controlled in project site by workers. As the general trend, the flow was increased, and the unit volume weight was decreased with increasing W/DM. Additionally, compressive strength and drying shrinkage were significantly influenced by W/DM. Hence, it can be stated that the adding water for dry cement mortar should be managed precisely since excessively increased W/DM for workability improvement can cause performance degradation of floor mortar with the failures such as excessive bleeding, and severe segregation during the fresh state. As a summary of the study, to achieve a desirable performance of dry cement mortar, approximately 20 % of W/DM can be suggested to be managed in project site.