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Development of a Model to Predict the Primary Infection Date of Bacterial Spot (Xanthomonas campestris pv. vesicatoria) on Hot Pepper

  • Kim, Ji-Hoon;Kang, Wee-Soo;Yun, Sung-Chul
    • The Plant Pathology Journal
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    • 제30권2호
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    • pp.125-135
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    • 2014
  • A population model of bacterial spot caused by Xanthomonas campestris pv. vesicatoria on hot pepper was developed to predict the primary disease infection date. The model estimated the pathogen population on the surface and within the leaf of the host based on the wetness period and temperature. For successful infection, at least 5,000 cells/ml of the bacterial population were required. Also, wind and rain were necessary according to regression analyses of the monitored data. Bacterial spot on the model is initiated when the pathogen population exceeds $10^{15}cells/g$ within the leaf. The developed model was validated using 94 assessed samples from 2000 to 2007 obtained from monitored fields. Based on the validation study, the predicted initial infection dates varied based on the year rather than the location. Differences in initial infection dates between the model predictions and the monitored data in the field were minimal. For example, predicted infection dates for 7 locations were within the same month as the actual infection dates, 11 locations were within 1 month of the actual infection, and only 3 locations were more than 2 months apart from the actual infection. The predicted infection dates were mapped from 2009 to 2012; 2011 was the most severe year. Although the model was not sensitive enough to predict disease severity of less than 0.1% in the field, our model predicted bacterial spot severity of 1% or more. Therefore, this model can be applied in the field to determine when bacterial spot control is required.

MODULATION OF TOXICITY AND CARCINOGENESIS BY CALORIC RESTRICTION

  • Allaben, William T.;Chou, Ming W.;Pegram, Rex A.;Leakey, Julian;Feuers, Ritchie J.;Duffy, Peter H.;Turturro, Angelo;Hart, Ronald W.
    • Toxicological Research
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    • 제6권2호
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    • pp.167-182
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    • 1990
  • Dietary restriction (caloric restriction) is the only intervention which has been reliably shown to extend the maximum life span of warm-blooded animals and delay the many phenomena associated with aging. It is also one of the most effective modulators of toxicity, especially cancer endpoints. In spite of the known modulator effects of caloric restriction, the biological mechanisms responsible for these effects had not been in vestigated until recently. The National Center for Toxicological Research (NCTR), in a collaborative effort with the National Institute of Aging (NIA), initiated a project whereby nine (9) combinations of rodent species/strains and diets were fed both restricted and ad libitum. The NIA's initiative was to identify biomarkers of aging whereas NCTR's initiative was to identify the biological effects associated with the profound effects caloric restriction has in protecting against both spontaneous (age-related) and chemically-induced toxic endpoints. Independent of sex or species, caloric restriction has similar effects on body temperature, oxygen consumption and $CO_2$production. Caloric restriction also decreased lipid glycolysis and metabolism in rats and mice, which suggest decreased production of metabolites which could lead to fatty acid epoxide formation. The age-associated loss of ciradian regulation of intermediate enzymes is also significantly reduced. Moreover, caloric restriction reduced the age-associated feminization of sexually dimorphic liver isozymes, increased several glucocorticoid responsive isozymes, elevated glucagon/insulin ratios, produced less microsomal superoxide and enhanced the capacity for utilzing detoxicating metabolic pathways. Calorically restricted rats have less than half the number of aflatoxin ($AFB_1$)-DNA adducts than ad libitum animals and urinary excretion of $AFB_1$ was increased significantly. Finally, DNA repair mechanisms are enhanced and oncogene expression is decreased in calorically restricted animals.

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훼손비탈면에 식재된 자생목본류의 묘유형별 생육특성에 관한 연구 (A Study on the Growth Characteristics under Seedling Types of Native Woody Plants After Planting on the Disturbed Slope)

  • 심상렬;김재환;정대영;문석기;고정현
    • 한국환경복원기술학회지
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    • 제13권2호
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    • pp.72-79
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    • 2010
  • This research was initiated to investigate the revegetation characteristics under seedling types of native woody plants on a disturbed slope. 4 different seedling types of woody plants (control, unit seedling plot, nature seedling plot, pot seedling plot) with the same woody plants (Rhus chinensis Mill, Albizzia julibrissin Duraz., Quercus acutissima Carr., Pinus densiflora S. et Z.) were treated with 3 replications on the experimental disturbed slope. Data such as the died number of native woody plants, growth height and visual quality were analyzed. The died number of plants were high in unit seedling plot when compared to the rest plots of seedling types. From the standpoint of died plant species, the died number were high in Rhus chinensis and Albizzia julibrissin whereas the died number were low in Quercus acutissima and Pinus densiflora. That's because we used 1-year old seedlings of Rhus chinensis and Albizzia julibrissin and 3-year old seedling Quercus acutissima and Pinus densiflora. This result indicated that the died reason of native woody seedling was not species but seedling age. Both growth height and visual quality were ranked first in the pot seedling plot, second in the nature seedling plot, third in the unit seedling plot, and last in the control plot, respectively. However, the difference of both growth height and visual quality in each seedling plot was not observed on the 11 months after planting. Especially, the main reason of low rooting and growth value in the unit seedling plot was due to wooven fabric outer crust of pot that obstruct seedling rooting into the soil. We concluded that pot seedling of native woody plants was effective in the viewpoint of the ecological disturbed slope revegetation.

RAPD를 이용한 고추(Capsicum annuum) 유전자원의 분류 (Classification of Capsicum annuum Germplasm Using Random Amplified Polymorphic DNA)

  • 남승현;최근원;유일웅
    • 원예과학기술지
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    • 제16권4호
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    • pp.503-507
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    • 1998
  • 본 연구는 RAPD표지를 이용하여 국내외에서 수집된 고추 유전자원들간의 유전적관계를 평가하고자 수행되었다. Random primer를 이용한 고추의 PCR반응은 $MgCl_2$ 3mM, Taq. DNA polymerase 1.5U, 주형 DNA 10ng, dNTPs $200{\mu}M$, random primer 200nM 그리고 $42^{\circ}C$의 annealing 온도조건으로 최적화하였다. 80개의 random primer로부터 높은 밴드선명도와 재현성을 보이는 16개가 선발되었으며 70%의 GC함량을 갖는 primer들이 GC함량이 60%인 것보다 DNA증폭에 있어 효과적이었다. 31개의 고추품종및 계통들에 대해 71개의 polymorphic밴드와 22개의 monomorphic밴드를 포함하는 총 93개의 DNA밴드가 선발된 16개의 random primer들로부터 형성되었다. Primer당 약 4.4개의 polymorphic밴드가 형성되었다. 이들 71개의 polymorphic밴드를 이용하여 유사도지수가 구해졌으며 이를 근거로 31고추 계통 또는 품종들을 뚜렷이 구분하는 dendrogram이 작성되었다.

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고강도 인바계 합금의 열팽창 및 인장 특성에 미치는 바나듐과 탄소 원소 첨가 영향 (Effects of V and C additions on the Thermal Expansion and Tensile Properties of a High Strength Invar Base Alloy)

  • 윤애천;윤신천;하태권;송진화;이기안
    • 소성∙가공
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    • 제24권1호
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    • pp.44-51
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    • 2015
  • The current study seeks to examine the effects of V and C additions on the mechanical and low thermal expansion properties of a high strength invar base alloy. The base alloy (Fe-36%Ni-0.9%Co-2.75%Mo-0.7Cr-0.23Mn-0.17Si-0.3%C, wt.%) contains $Mo_2C$ carbides, which form as the main precipitate. In contrast, alloys with additions of 0.4%V+0.3%C (alloy A) or 0.4%V+0.45%C (alloy B) contain $Mo_2C$+[V, Mo]C carbides. The average thermal expansion coefficients of these high strength invar based alloys were measured in the range of $5.16{\sim}5.43{\mu}m/m{\cdot}^{\circ}C$ for temperatures of $15{\sim}230^{\circ}C$. Moreover, alloy B showed lower thermal expansion coefficient than the other alloys in this temperature range. For the mechanical properties, the [V, Mo]C improved hardness and strengths(Y.S. and T.S.) of the high strength invar base alloy. T.S.(tensile strength) and Y.S.(yield strength) of hot forged alloy B specimen were measured at 844.6MPa and 518.0MPa, respectively. The tensile fractography of alloy B exhibited a ductile transgranular fracture mode and voids were initiated between the [V, Mo]C particles and the matrix. Superior properties of high strength and low thermal expansion coefficient can be obtained by [V, Mo]C precipitation in alloy B with the addition of 0.4%V and 0.45%C.

가속성능이 우수한 외팔보형 고속예인전차의 설계 (On the Design of Cantilever Type High Speed Towing Carriage with Excellent Acceleration Performance)

  • 김재성;김효철
    • 대한조선학회논문집
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    • 제53권3호
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    • pp.228-236
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    • 2016
  • Extraordinarily the establishment of towing tank has been initiated after the allocation of space at the basement floor of existing building through remodeling procedure. Therefore the asymmetric tank should be unavoidably determined by compromising with the allowable space and existing building structure. Consequently the shape of towing carriage ought to be selected as a cantilever type to match with the given environmental conditions. Finally the major role of the towing tank has been configured on the fundamental research work for the high speed marine vehicles. Due to the limited length of towing tank, it is appeared that the carriage should accelerated with 1.2m/sec2 which is equivalent to twice of the maximum acceleration in ordinary practices on design application of carriage. In such a condition the exerted total power of motor could not be converted to traction force of the carriage without slip for the acceleration. To overcome these difficulties the contact pressure of a horizontal traction wheel to rail has been reinforced by the elastic recovery force of springs on supporting rollers. It is believed that the design experience of the high speed towing carriage under unusual circumferential condition and acceleration barrier could be utilized not only on the design of high speed towing carriage but also on the improvement of existing facilities.

PROTO-TYPE INSTALLATION OF A DOUBLE-STATION SYSTEM FOR THE OPTICAL-VIDEO-DETECTION AND ORBITAL CHARACTERISATION OF A METEOR/FIREBALL IN SOUTH KOREA

  • Hinse, Tobias C.;Kim, Woo-Kyum;Ahn, Sang-Hyeon;Lee, Jae-Keun;Park, Jun-Hyeong;Lee, Young-Woo;Jeong, Woo-Jung;Woo, Sang-Min
    • 천문학논총
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    • 제32권3호
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    • pp.381-405
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    • 2017
  • We give a detailed description of the installation and operation of a double-station meteor detection system which formed part of a research & education project between Korea Astronomy Space Science Institute (KASI) and Daejeon Science Highschool. A similar system is currently not existing in South Korea. A total of six light-sensitive CCD cameras were installed with three cameras at SOAO and three cameras at BOAO observatory. A double-station observation of a meteor event enables the determination of the three-dimensional heliocentric orbit in space. This project was initiated in response to the Jinju fireball event in March 2014. The cameras were installed in October/November 2014. The two stations are identical in hardware as well as software. Each station employes sensitive "Watec-902H2" cameras in combination with relatively fast f/1.2 lenses. Various fields of views were used for measuring differences in detection rates of meteor events. We employed the SonotaCo UFO software suite for meteor detection and their subsequent analysis. The system setup as well as installation/operation experience is described and first results are presented. We also give a brief overview of historic as well as recent meteor (fall) detections in South Korea. For more information please consult http://meteor.kasi.re.kr.

수면 중 호흡의 조절 (Control of Ventilation during Sleep)

  • 김우성
    • 수면정신생리
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    • 제6권1호
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    • pp.19-25
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    • 1999
  • Sleep alters both breathing pattern and the ventilatory responses to external stimuli. These changes during sleep permit the development or aggravation of sleep-related hypoxemia in patients with respiratory disease and contribute to the pathogenesis of apneas in patients with the sleep apnea syndrome. Fundamental effects of sleep on the ventilatory control system are 1) removal of wakefulness input to the upper airway leading to the increase in upper airway resistance, 2) loss of wakefulness drive to the respiratory pump, 3) compromise of protective respiratory reflexes, and 4) additional sleep-induced compromise of ventilatory control initiated by reduced functional residual capacity on supine position assumed in sleep, decreased $CO_2$ production during sleep, and increased cerebral blood flow in especially rapid eye movement(REM) sleep. These effects resulted in periodic breathing during unsteady non-rapid eye movement(NREM) sleep even in normal subjects, regular but low ventilation during steady NREM sleep, and irregular breathing during REM sleep. Sleep-induced breathing instabilities are divided due primarily to transient increase in upper airway resistance and those that involve overshoots and undershoots in neural feedback mechanisms regulating the timing and/or amplitude of respiratory output. Following ventilatory overshoots, breathing stability will be maintained if excitatory short-term potentiation is the prevailing influence. On the other hand, apnea and hypopnea will occur if inhibitory mechanisms dominate following the ventilatory overshoot. These inhibitory mechanisms include 1) hypocapnia, 2) inhibitory effect from lung stretch, 3) baroreceptor stimulation, 4) upper airway mechanoreceptor reflexes, 5) central depression by hypoxia, and 6) central system inertia. While the respiratory control system functions well during wakefulness, the control of breathing is commonly disrupted during sleep. These changes in respiratory control resulting in breathing instability during sleep are related with the pathophysiologic mechanisms of obstructive and/or central apnea, and have the therapeutic implications for nocturnal hypoventilation in patients with chronic obstructive pulmonary disease or alveolar hypoventilation syndrome.

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S&T Collaboration in Developing Countries: Lessons from Brazilian Collaboration Activities with South Korea

  • Fink, Daniel;Hameed, Tahir;So, Minho;Kwon, Youngsun;Rho, Jae Jeung
    • STI Policy Review
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    • 제3권2호
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    • pp.92-110
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    • 2012
  • An active policy role is suggested for collaboration that will enhance national level Science and Technology (S&T) capabilities. In contrast, some studies see a limited role of policy support for collaborative S&T research at the individual level (including research groups or institutional levels) due to their self-organizing nature. Two views on S&T collaboration look at different levels but existing research largely ignores the context of developing countries. This paper shows how S&T collaborations between Brazil and Korea have developed over the past two decades. The paper indicates the gaps between initiation and the actual establishment of collaborative research at national and individual levels, differences in the focus areas of research, differences in resources and project planning, and the nature of collaborations. Collaborative research activities at the national level were initiated before the individual level but activated later than the individual level; in addition, the focus areas of research were laid down earlier at the national level with individual collaborations focused on different areas. Project types remained different at each level (i.e. top-down and bottom-up) or with a slightly changing mix. This study suggests appropriate policy measures (such as the timely and effective information collection of activities at different levels and proactive coordination) that could reduce the gaps in the timing and alignment of research areas. This paper also alludes to an evolutionary model to develop S&T collaboration among developing countries.

Properties of the Microinterface formed by Phosphatidylcholine and 1-Butanol as Reaction Media of Hydrolysis of Phosphatidylcholine

  • Yamazaki, Keiju;Imai, Masanao;Suzuki, Isao
    • 한국막학회:학술대회논문집
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    • 한국막학회 2004년도 Proceedings of the second conference of aseanian membrane society
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    • pp.82-85
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    • 2004
  • Microinterface of W/Omicroemulsion prepared by phosphatidylcholine was used as reaction media of hydrolysis of phosphatidylcholine by phospholipaseA$_2$. Phosphatidylcholine was used as an amphiphile and was acted as a substrate. Organic phase of W/Omicroemulsion in this study was prepared by mixed organic solvents i.e. 2,2,4-trimethylpentane (isooctane) as a main solvent and 1-butanol as a co-solvent. The effect of added 1-butanol was remarkable not only on reaction beginning but also on high reaction rate. The hydrolysis reaction was dramatically initiated when 1-butanol was injected into the running isooctane/PC system. The enhancement by 1-butanol addition into single organic solvent was our original finding compare with previous conventional organic solvent. The reaction rate was elevated by the added amount of 1-butanol. The enhanced reaction rate was about 150-folds. This enhancement was speculated as 1-butanol adsorption on the microinterface. The adsorbed 1-butanol improved the properties of microinterface, especially its mobility was increased by difference of the chain length between phosphatidylcholine and 1-butanol. PhospholipaseA$_2$ molecules were located on the microinterface due to modified mobility of microinterface. Located phospholipaseA$_2$ on the microinterface reacted easily with phosphatidylcholine molecule. As a result high reaction rate was obtained. Microinterfacial properties were successfully improved by adsorbed 1-butanol molecule, and were favorable to appear higher reactivity of phospholipaseA$_2$.

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