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The study of seed morphological trait and testa characteristic for Korean Vicia species

  • Han, Se-Hui;;Kim, Seong-Hun;Hyeon, Do-Yun;Lee, Gyeong-Jun;Lee, Jeong-Ro;Jo, Gyu-Taek
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.64-64
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    • 2019
  • In order to evaluate the taxonomical relevance of seeds morphological characteristics, a macroand micro-morphological study was conducted on seeds of Korean Vicia (Fabacea). We collected 19 taxa of genus Vicia distributed in Korea and introduced one taxa from USDA. The morphological characteristic and testa texture of seeds were investigated using a Stereo-microscope (SM) and Scanning Electron Microscope (SEM). Most of Vicia seeds were found spherical or oblong and some seeds were oval and subglose. The largest seed was V. chosenensis ($4.3{\times}3.6{\times}2.6mm$), and the smallest was V. teterasperma ($1.7{\times}1.7{\times}1.5mm$). V. chosenensis and V. hirsuta were separated from other Vicia species by having a shiny in seed finish. In hilum shape, 14 species have linear and V. sepium was distinguished by having a circumlinear. In testa texture, they developed papilae, only V. hirsuta has lophate in level type. Deposition of the sheet-like debris between the papilae was observed in V. chosenensis, V. cracca, and V. unijuga. Polymorphism information content (PIC) values of the 13 qualitative morphological characters (QMC) were in the range of 0.0950 to 8863 with an average of 0.4611. PIC value of seed shape, seed colour, hilum colour were 0.7403, 0.8177, 0.883 respectively. Cluster analysis based on QMC detected three main clades. V. cracca, V. amurensis, V. amoena were involved in Group 1 and V. unijuga f. minor, V. unijuga, V. unijuga f. angustifolia, V. sepium, V. hirticalycina, V. hirsuta, V. linearfolia, V. chosenensis, V. pseudorobus, V. venosa var. cuspidata were involved in Group 2. V. nipponica, V. japonica, V. villosa, V. dasicarpa, V. bungei, V. angustifolia, V. tetrasperma were clustered in Group 3. Our research suggests that morphological characteristic and testa texture of seeds could be used as definers for the identification of genus Vicia.

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Technology Convergence & Trend Analysis of Biohealth Industry in 5 Countries : Using patent co-classification analysis and text mining (5개국 바이오헬스 산업의 기술융합과 트렌드 분석 : 특허 동시분류분석과 텍스트마이닝을 활용하여)

  • Park, Soo-Hyun;Yun, Young-Mi;Kim, Ho-Yong;Kim, Jae-Soo
    • Journal of the Korea Convergence Society
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    • v.12 no.4
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    • pp.9-21
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    • 2021
  • The study aims to identify convergence and trends in technology-based patent data for the biohealth sector in IP5 countries (KR, EP, JP, US, CN) and present the direction of development in that industry. We used patent co-classification analysis-based network analysis and TF-IDF-based text mining as the principal methodology to understand the current state of technology convergence. As a result, the technology convergence cluster in the biohealth industry was derived in three forms: (A) Medical device for treatment, (B) Medical data processing, and (C) Medical device for biometrics. Besides, as a result of trend analysis based on technology convergence results, it is analyzed that Korea is likely to dominate the market with patents with high commercial value in the future as it is derived as a market leader in (B) medical data processing. In particular, the field is expected to require technology convergence activation policies and R&D support strategies for the technology as the possibility of medical data utilization by domestic bio-health companies expands, along with the policy conversion of the "Data 3 Act" passed by the National Assembly in January 2019.

Analysis on the Key Factors of Entrepreneurship Education for Public Technology Commercialization : Focusing on the Performance of Korean I-Corps Project (공공기술 사업화를 위한 창업교육의 핵심요인 분석 : 한국형 아이코어 사업성과를 중심으로)

  • Lee, Won-Cheul;Choi, Jong-In;Choi, Tae-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.1
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    • pp.159-170
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    • 2021
  • As the main purpose of R&D changes from the center of knowledge creation to the center of economic value creation through technology transfer and commercialization, public technologies can also secure economic feasibility as well as make a social contribution. Korea has been focusing on fostering core human resources who can lead the commercialization of basic and original research results by launching the 'Support project for exploring startups linked with public technology-based markets' since 2015 in order to promote public technology startup. This study is based on the results of a survey for the purpose of analyzing the performance of this project. In addition, this study derived four factors related to the I-corps project performance from the results of this survey and verified the relationship between these factors through structural equation model analysis. In summary, it was confirmed that 'Application Level' and 'Business Model,' which are positively affected directly from 'Entrepreneurship Learning,' have positive effects on 'Financial Resources'. Furthermore, the indirect effect of 'Entrepreneurship Learning' on 'Financial Resources' was verified. In particular, the high level of impact of 'Entrepreneurship Learning' on 'Application Level,' and the impact of 'Application Level' on 'Business Model' and 'Financial Resources' were also positive.

High-density single nucleotide polymorphism chip-based conservation genetic analysis of indigenous pig breeds from Shandong Province, China

  • Wang, Yanping;Zhao, Xueyan;Wang, Cheng;Wang, Wenwen;Zhang, Qin;Wu, Ying;Wang, Jiying
    • Animal Bioscience
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    • v.34 no.7
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    • pp.1123-1133
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    • 2021
  • Objective: Shandong indigenous pig breeds are important Chinese pig resources. Their progressive population decline in recent decades has attracted attention towards their conservation. Conservation genetics of these indigenous breeds are essential for developing a conservation and utilization scheme. Methods: A high-density single nucleotide polymorphism (HD-SNP) chip-based comparative analysis of genetic characteristics was performed for seven Shandong indigenous pig breeds in the context of five Western commercial breeds. Results: The results showed that Shandong indigenous pig breeds varied greatly in genetic diversity, effective population size, inbreeding level, and genetic distance with the Western commercial breeds. Specifically, Laiwu and Dapulian displayed low genetic diversity, and had a genetically distant relationship with the Western commercial breeds (average F statistics [FST] value of 0.3226 and 0.2666, respectively). Contrastingly, the other five breeds (Yantai, Licha, Yimeng, Wulain, and Heigai) displayed high genetic diversity within breed and had some extent of mixture pattern with the Western commercial breeds, especially Duroc and Landrace (FST values from 0.1043 to 0.2536). Furthermore, intensive gene flow was discovered among the seven Shandong indigenous breeds, particularly Wulian, Licha, and Heigai, as indicated by the large cluster formed in the principal component analysis scatterplot and small population differentiation (average of 0.1253) among them. Conclusion: Our study advances the understanding of genetic characteristics of Shandong indigenous breeds and provides essential information for developing an appropriate conservation and utilization scheme for these breeds.

Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

  • Wang, Laiyou;Guo, Shuxian;Zeng, Bo;Wang, Shanshan;Chen, Yan;Cheng, Shuang;Liu, Bingbing;Wang, Chunyan;Wang, Yu;Meng, Qingshan
    • Mycobiology
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    • v.50 no.1
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    • pp.66-78
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    • 2022
  • The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key economic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequencing data or associated annotations for this strain are available at present, it remains challenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic complete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mechanistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

Antioxidant activity of ten Lamiaceae plant seed extracts (10종 꿀풀과(Lamiaceae) 식물 종자 추출물의 항산화 활성)

  • Kim, JunHyeok;Lee, Hee Ho;Park, Chung Youl;Kim, Hyun Min;Jung, Young Ho;Kim, Sae Hyun;Na, Chae Sun
    • Journal of Applied Biological Chemistry
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    • v.65 no.3
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    • pp.121-128
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    • 2022
  • This study explored plant-derived natural antioxidants by evaluating the antioxidant activity of Lamiaceae plant seed extracts. Plants with the percentage of filled seeds at or above 90% and seed germination at or above 50% were selected. Of the ten species studied, the total phenolic content of the seeds was high in the species Phlomis umbrosa Turcz. (6.2 mg GAEs/g of seeds) and Elsholtzia ciliata (Thunb.) Hyl. (4.5 mg GAEs/g of seeds). The total flavonoid content of the seeds was high in E. ciliata (1.0 mg QEs/g of seeds) and P. umbrosa (0.6 mg QEs/g of seeds). Based on the EC50 value of the seed extracts, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was high in the seeds of the plants E. ciliata (27.5 ㎍/mL), Mosla dianthera (Buch.-Ham. ex Roxb.) Maxim. (29.2 ㎍/mL), and Prunella vulgaris var. lilacina Nakai (33.3 ㎍/mL). In addition, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity was high in P. vulgaris var. lilacina (25.6 ㎍/mL), E. ciliata (25.9 ㎍/mL), and M. dianthera (27.6 ㎍/mL) seeds. The ferric reducing antioxidant power of the seed extracts was high in P. vulgaris var. lilacina (2910.4 µM Fe(II)/g of extract), E. ciliata (2836.2 µM Fe(II)/g of extract), and M. dianthera (2754.4 µM Fe(II)/g of extract). According to the cluster analysis based on antioxidant activity, the seeds of the ten species were classified into three groups, from group 1 with low antioxidant activity to group 3 with high antioxidant activity; E. ciliata, M. dianthera, and P. vulgaris var. lilacina were classified as group 3.

Characterization of Heavy Metal Pollution in Sediments of Major Reservoirs in South Korea (우리나라 주요 호소의 퇴적물 내 중금속 오염도에 따른 특성 분석)

  • Yun Sang Jeong;Dae-Seong Lee;Da-Yeong Lee;Ihn-Sil Kwak;Young Seuk Park
    • Korean Journal of Ecology and Environment
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    • v.55 no.2
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    • pp.175-183
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    • 2022
  • In this study, 46 reservoirs in South Korea were characterized based on heavy metal concentration in sediments. We analyzed the relationship between heavy metal concentrations, physicochemical water quality and hydromorphological factors in each reservoir. Study reservoirs were classified into five groups of reservoirs, by hierarchical cluster analysis based on the similarities of heavy metal concentration. Group 1 had the most severe sediment heavy metal contamination among the groups, whereas Groups 2 and 3 showed low levels of heavy metal contamination. Group 4 displayed high value of Ni, and Group 5 showed high contamination of Pb, Cu, Cr, Ni, and Hg. Groups 1 and 5, which had high concentration of heavy metals in sediments, showed a high density of mines in the catchment of reservoirs. Heavy metal concentration was high in reservoirs with large capacity or the ones located at higher elevation, and also highly related with number of mines in the catchment of reservoir. This study can contribute to the systematic management of sediment heavy metals in reservoirs.

A Genome-wide Association Study of Preferred Primal Cuts of Hanwoo Cattle Using Single-step GBLUP (한우 부분육 선호부위에 대한 ssGBLUP을 활용한 GWAS 분석)

  • Lee, Jae Gu;Park, Byoungho;Park, Mi Na;Alam, M.;Kim, Sidong;Do, Changhee;Choi, Tae Jeong
    • Journal of agriculture & life science
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    • v.50 no.3
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    • pp.99-117
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    • 2016
  • Data on primal cuts were collected from 1,829 steers of Hanwoo progeny testing programs, between 2010 and 2015 for the ssGWAS. SNP data were analyzed by using Illumina Bovine 50K Beadchip. The SNP data that matches with phenotype data was 674 animals. As a first step, the genomic estimated breeding value(GEBV) of the loin and rib cuts were estimated, which was used in the estimation of SNP marker effects and their variances related to the traits. Then, the estimated variance explained by each marker was expressed as a proportion to the total genetic variance. Finally, the SNP loci and their significance to any possible QTL were examined. Among the 20 best SNP loci explaining a larger proportion of SNP variance to the total genetic variance for tender loin yield, the region between 12,812,193 ~ 12,922,313bp on BTA 10 harbored a cluster of SNPs that explained about 7.32 to 7.34% of the total genetic variance. For strip loin yield, a peak for higher effects for multiple SNPs was found in BTA24, between 38,158,543 and 38,347,278bp distances, which explained about 8.36 to 8.56% of the observed variance for this trait. For loin yield had relatively smaller effects in terms of the total genetic variance. Therefore, loin yield might be affected by a few loci with moderate effects and many other loci with smaller effects across the genome.

The isobaric effect on the measurement of Gd isotopes by thermal ionization mass spectrometry (열이온화 질량분석기를 이용한 Gd 동위원소 측정에서 동중원소 영향)

  • Jeon, Young-Shin;Park, Jong-Ho;Joe, Kih-Soo;Han, Sun-Ho;Song, Kyuseok
    • Analytical Science and Technology
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    • v.22 no.5
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    • pp.415-421
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    • 2009
  • A study was performed to investigate the isobaric effects of impurities in isotope measurement of gadolinium by TIMS. Especially, the ratio of $^{155}Gd/^{158}Gd$ showed the higher value than that of natural gadolinium and also decreased as the measuring time increased. This phenomenon is considered to be due to the oxide form of La ($LaO^+$, m/z=155) causing to create a serious bias on the measurement of $^{155}Gd$ abundance by La as an impurity, and due to $LaO^+$ produced more than the $Gd^+$ in the early time which disappears as the time goes on because of lower melting point and ionization potential of La than Gd. Although isobaric effects from Ba($BaO^+$), Ce($CeO^+$), Sm($SmO^+$), La($LaO^+$), and $K_4{^+}$(m/z=156)-cluster were detected even when blank rhenium filaments were used, these could be avoided by preconditioning(baking out) the filament. And we found that the measurement of $GdO^+$ instead of $Gd^+$ is more suitable in avoiding the isobaric effect from impurities such as La, Ce and Ba in the measurement of Gd isotope only in case of absence or extremely low level of Yb, Sm, Dy, Er, Lu.

Analysis of Regional Economic Ripple Effects of Port Logistics Industry in Gwangyang City - Focusing on Exogenous Specified Input-Output Model - (광양시 항만물류산업의 지역경제 파급효과 분석 - 외생화 산업연관모형을 중심으로 -)

  • Kim, Min-Seong;Na, Ju-Mong
    • Journal of Korea Port Economic Association
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    • v.39 no.2
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    • pp.77-95
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    • 2023
  • The regional infrastructure industries of Gwangyang City, the subject of this study, are Gwangyang Port and Gwangyang Steel Mill. Therefore, it is necessary to analyze the regional economic ripple effects of the port logistics industry in Gwangyang City. In this study, a multi-stage approach using the RW and the LQ methodology using the national input-output tables in 2015 and 2019 is used to prepare the regional interindustry analysis chart in Gwangyang City, and an exogenous demand induction model that reclassified the port logistics industry was applied. Through this, the purpose of this study was to provide policy implications by figuring out the regional economic ripple effects of the port logistics industry quantitatively in Gwangyang City. As a result of the analysis, the industries with high production inducement effect and forward/backward linkage effect of the port logistics industry in Gwangyang City were analyzed as manufacturing, transportation, land and air logistics sectors. And the industries in which the added value inducement effect and the employment inducement effect were analyzed as an industry related to the service industry. Therefore, it is necessary to prepare support measures to foster the port logistics industry as a way to promote these industries and revitalize the local economy of Gwangyang City. To this end, it is desirable to improve policies and systems for the vitalization of the Gwangyang port maritime cluster and provide various policy support for the port logistics industry in Gwangyang City. This study is meaningful in suggesting policy implications for the regional economy of Gwangyang City based on the results of exogenous analysis of the port logistics industry in small and medium-sized cities. However, It seems that further studies related to this will be needed in the future.