• 한국균학회지
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• 제15권1호
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• pp.9-13
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• 1987

한국산 백복령을 chloroform-methanol(2 : 1, v/v) 혼합용매로 추출한 다음, 비누화 및 메델에스테르화 한 후 gas chromatography에 의하여 얻은 총24개의 Peak 중, caprylic acid($C_{8:0}$), undecanoic acld($C_{11:0}$) lauric acid($C_{12:0}$) , dodecenoic acid($C_{12:1}$) 및 Palmitic acid($C_{16:0}$)의 5 개 성분을 확인하였으며, 그중 주성분인 $C_{8:0}$과 $C_{12:1}$은, 시료 g당 $6.657{\mu}g$ 및 $10.176{\mu}g$ 이었다.

• Applied Biological Chemistry
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• 제42권3호
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• pp.262-266
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• 1999

국내 식물자원 30 가지의 항산화활성을 탐색한 결과 방아잎, 형개, 택란의 methanol 추출물에서 높은 활성을 확인하였다. 그 중에서 항산화활성이 가장 높은 방아잎의 건조분말을 methanol로 환류냉각 추출하여 hexane chloroform, ethyl acetate, water 순으로 용매분획한 결과 ethyl acetate 분획에서 가장 높은 항산화활성을 확인하였으며, 이를 농축한 다음 Sephadex LH-20으로 chromatography하여 순수물질을 얻었다. 이물질을 ESIMS로 분석한 결과 분자량이 360임을 확인하였고, IR과 UV spectra에 의해서 phenol계 화합물임을 확인하였으며 NMR 분석에 의해서 이 항산화물질이 rosmarinic acid임을 확인하였다. 이 물질의 항산화활성을 검정한 결과 돈지와 팜유에 대해서 BHT나 ${\alpha}-tocopherol$보다도 우수한 지질과산화 억제효과를 나타냈다. 방아잎의 methanol추출물의 항산화력은 rosmary의 methanol 추출물과는 비슷하였으나, sage의 methanol추출물 보다는 높게 나타났다.

• 한국미생물·생명공학회지
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• 제40권4호
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• pp.380-388
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• 2012

북극 지의류로부터 서로 다른 5종의 극지 미생물을 분리하였고, 그들의 생리활성 물질들은 아세톤, 물, 클로로포름, 다이에틸에테르, 에탄올, 에틸아세테이트, 메탄올 및 페트롤륨 에테르 등 다양한 용매를 이용하여 균 배양액으로부터 추출되었다. 이러한 추출물들의 항균성은 Staphylcoccus aureus, Bacillus subtilis, Micrococcus luteus, Enterobacter cloacae, Pseudomonas aeruginosa 및 Escherichia coli 등 6종의 병원체에 대한 디스크 확산법과 최소억제농도 측정법에 의해 조사되었다. 여러 추출 샘플 중, Burkholderia sordidicola S5-$B^T$ (KOPRI 26644) 유사미생물 종의 에틸아세테이트 추출물이 표적박테리아에 대한 높은 항균활성을 나타내었다(억제대, 7-10 mm; 최소억제농도, $57.5{\rightarrow}1000{\mu}g/ml$). 또한, 다양한 추출 용매 중, 클로로포름 추출물에서 약하지만 분명한 활성을 나타내었다.

• Natural Product Sciences
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• 제13권1호
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• pp.54-60
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• 2007

The plant Ichnocarpus frutescens (Linn) R.Br. (Family-Apocynaceae) has been indicated for the treatment of various diseases, one amongst it is cancer. The purpose of this study was to investigate experimentally the possible antitumor activity and antioxidant role of Ichnocarpus frutescens in the mice transplanted with Ehrlich ascites carcinoma (EAC). The chloroform and methanol extract of whole plant of Ichnocarpus frutescens (CEIF and MEIF) were administered intraperitoneally at the dose of 150 mg/kg and 300 mg/kg, body weight per day for 7 days after 24 h of tumor inoculation in mice. Treatment with CEIF at the dose of 150 mg/kg and 300 mg/kg remarkably decreased the tumor volume, packed cell volume, viable cell count and increased the nonviable cell count of EAC tumor bearing mice when compared to e effect of MEIF at 150 mg/kg and 300 mg/kg. Further the EAC mice treated with CEIF and MEIF showed significant decrease in the level of lipid peroxidation and significant increase in the level of antioxidant enzymes such as glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT), however the decreasing and increasing capacity of CEIF was less in both doses as compared to MEIF. Based on these results, it can be concluded that the chloroform and methanol extact of Ichnocarpus frutescens exhibit significant antitumor and antioxidant activity in EAC bearing mice.

• Advances in Traditional Medicine
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• 제7권5호
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• pp.477-484
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• 2008

The present study was aimed at investigating the hypouricemic and xanthine oxidase inhibitory activities of the various fractions of the hydromethanolic extract of the leaves of Coccinia grandis L. Voigt (Cucurbitaceae). The leaves of this species was used in traditional medicinal system for the treatment of gout, rheumatism, jaundice, bronchitis, fever, skin eruptions, wounds, etc. The degree of xanthine oxidase inhibition was determined in vitro by measuring the increase in absorbance at 295 nm associated with uric acid formation. Among the fractions tested, the chloroform fraction exhibited highest potency ($IC_{50}$ $17.8\;{\mu}g/ml$). This was followed by the pet-ether ($IC_{50}$ $29.7\;{\mu}g/ml$), ethyl acetate ($IC_{50}$ $41.2\;{\mu}g/ml$) and residual ($IC_{50}$ $47\;{\mu}g/ml$) fractions. The $IC_{50}$ value of allopurinol was $6.1\;{\mu}g/ml$. In addition, the hypouricemic and hepatic xanthine oxidase (XO)/xanthine dehydrogenase (XDH) inhibitory activities of the fractions were examined in vivo using oxonate (280 mg/kg, i.p.) induced hyperuricemic mice. At a dose of 200 mg/kg orally for 7 days, the pet-ether, chloroform and ethyl acetate fractions produced a significant (P < 0.01) reduction in serum urate level and also inhibited hepatic XO/XDH activities when compared to hyperuricemic mice. These inhibitory effects were weaker than that observed for the standard drug, allopurinol (10 mg/kg, p.o.). Lineweaver-Burk analysis of the enzyme kinetics indicated that the mode of inhibition was of a mixed type. These results suggest that the use of Coccinia grandis leaves for the treatment of gout could be attributed to its XO inhibitory activity.

• KSBB Journal
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• 제29권1호
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• pp.29-35
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• 2014

In this study, antioxidant and antimicrobial activities of ethanolic crude extract and its five different solvent subfractions (namely, ethyl acetate fraction, n-butanol fraction, chloroform fraction, n-hexane fraction and the aqueous fraction) from a mixture of four different medicinal herbs (Galla rhois, Achyranthes japonica Nakai, Terminalia chebula Retz and Glycyrrhiza uralensis) were investigated. Among all the tested mixture combination of four medicinal herbs, 5:3:1:1 ratio of Galla:Achyranthes : Terminalia : Glycyrrhiza had the best antimicrobial effects against four strains of microorganisms (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli) and exhibited the highest DPPH radical-scavenging activity. Further sub-fractions with solvents were screened for antioxidant and antimicrobial activities. Antioxidant activity in order was ethyl acetate fraction > n-butanol fraction > chloroform fraction > nhexane fraction > aqueous fraction. The n-butanol extracted fraction showed the highest level of antimicrobial activity in com- parison to other fractions. In addition, all those fractions did not show any cytotoxicity against human skin cell CCD-986sk. These results suggest that 5:3:1:1 combination extracts of medicinal herbs (Galla : Achyranthes : Terminalia : Glycyrrhiza) may be potentially used as a safe natural antimicrobial preservative.

• 한방비만학회지
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• 제15권2호
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• pp.111-122
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• 2015

Objectives: Schizandrae fructus (Schizandra chinensis) is one of very common herbs, it is known as natural antioxidants, anti-inflammatory agent. Also some reports show that its extract works to regulate of dyslipidemia. This study was designed to investigate the effects of Schizandrae fructus chloroform fraction (SFCF) on serum lipid levels in dyslipidemic mice. Methods: The levels of total cholesterol, high density lipoprotein-cholesterol, triglyceride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), fasting blood glucose in serum were measured. Histopathological and gene expression changes in liver tissue were also observed. Results: Oral administration of SFCF lowered levels of total cholesterol and triglyceride, which were elevated by high-fat diet. But SFCF did not affect on weight changes and serum AST, ALT levels in dyslipidemic mice. After carrying out gene ontological analysis, large numbers of genes in high-fat diet group were up-(347) or down-regulated (235). In SFCF treated mice, some changed expression of the genes was restored to normal levels, with a recovery rate of 17%. And it seems that fatty acid biosynthesis pathway was one of important key pathways to recovery. Conclusions: SFCF has beneficial effect on dyslipidemia, and could be used to prevent and treat cardiovascular disease.

• 한국식품저장유통학회지
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• 제11권4호
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• pp.461-467
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• 2004

장기간 자연숙성시킨 전통된장의 항산화 효과는 숙성기간이 길어질수록 대체로 감소되었으며, 숙성기간 1년 된장에서 methanol 분획물이 가장 우수하였으며, 다음으로 hexane과 물 분획물의 순이었다. 지용성 및 수용성 추출물은 숙성기간이 길어질수록 항산화 효과가 점진적으로 오히려 증가되었으나, 분획물에 비하여 낮은 수치를 나타내었다. 전통된장의 수소공여능은 수용성 색소 추출물과 methanol 및 butanol 분획물에서 대체적으로 높은 수치였으나, chloroform과 ethylacetate 분획물은 매우 낮았다. 전통된장의 항암효과에서 인체 폐암 세포주(A549)는 물 분획물에서 가장 높았고, 인체 유방암 세포주(MCF-7)는 methanol 분획물에서 가장 높은 수치를 나타내었으며, 특히 전통된장의 숙성기간이 길어질수록 암세포 성장억제효과는 높게 나타났다.

• 한국식품과학회지
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• 제30권3호
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• pp.672-679
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• 1998

고삼 75% 에탄올 추출물과 클로로포름 분획물을 첨가하여 몇가지 식중독 미생물의 증식억제 양상과 분리된 유효 물질의 구조를 확인하였다. 이 분획물의 항균 효과를 시험한 결과 Listeria monocytogenes (ATCC 19111, 19112, 19113, 19114 및 15313)에 대한 최소 증식억제 농도가 각각 $50{\sim}500\;ppm$과 50 ppm 이하로 확인되었다. 항균 활성을 보인 클로로포름 분획물을 silica gel column chromatography로 연속 2회 정제하여 얻은 황색 분말의 항균 활성 소획분 S-10-6은 L. monocytogenes 5 균주, Bacillus subtilis 및 Staphylococcus aureus에 대해서는 10 ppm 농도에서 뚜렷한 증식억제 효과를 보였으며 특히 L. monocytogenes 5균주에 대해서는 $30{\sim}50\;ppm$ 수준에서 살균 효과가 인정되었으나 E. coli 경우 100 ppm 농도에서도 증식억제 효과가 없었다. 항균 활성을 보인 소획분(S-10-6)을 IR, $^1H-NMR$ 및 $^{13}C-NMR$로 구조를 동정한 결과 flavanone 화합물의 하나인 kushenol F로 확인되었다.

• Food Science and Biotechnology
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• 제16권1호
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• pp.163-166
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• 2007

The cytotoxic effects of partially purified substances from Bacillus polylfermenticus SCD toward a variety tumor cell lines were studied. Cytotoxic activity was determined with regard to the A549 (human lung carcinoma), AGS (human stomach adenocarcinoma), DLD-1 (human colon adenocarcinoma), HEC-1-B (human uterus adenocarcinoma), SW-156 (human kidney carcinoma), and NIH/3T3 (murine normal fibroblast) cell lines using the MTT assay. Cytotoxic substances were partially purified through Diaion HP-20 columns and extracted with methanol or other organic solvents (n-hexane, chloroform, ethylacetate, and butanol). B. polyfermenticus SCD supernatant showed up to 60% inhibition of cell viability fer all five human cancer cell lines tested. When treated with 10 mg/mL of n-hexane, chloroform, ethylacetate, and butanol extract, HEC-1-B cells showed a 25,62,35, and 63% rate of inhibition respectively, and AGS cells showed a 72, 61, 44, and 67% rate of inhibition, respectively. At a concentration of 10 mg/mL, 100% methanol Diaion HP-20 extracts showed inhibition rates of 97.0% toward A-549 cells, 98.1% toward AGS cells, 81.6% toward DLD-1 cells, 83.5% toward HEC-1-B cells, and 92.7% toward SW-156 cells. These results indicate that partially purified fractions from B. polyfermenticus SCD have the potential to inhibit not only colon cancer cells, but also lung, stomach uterus, and kidney cancer cells. Further studies are needed to characterize the cytotoxic substances released in B. polyfermenticus SCD cultures.