Browse > Article
http://dx.doi.org/10.7841/ksbbj.2014.29.1.29

Antioxidant and Antimicrobial Activities of Combined Extracts of Galla rhois, Achyranthes japonica Nakai, Terminalia chebula Retz and Glycyrrhiza uralensis  

Cho, Hong-Suk (Research Institute, World Costec. Co. Ltd.)
kang, Se-Won (Research Institute, World Costec. Co. Ltd.)
Kim, Ju-Hee (Research Institute, World Costec. Co. Ltd.)
Choi, Min-Joo (Research Institute, World Costec. Co. Ltd.)
Yu, Hye-Won (Research Institute, World Costec. Co. Ltd.)
Park, Euteum (Department of Biotechnology, Chosun University)
Chun, Hong Sung (Department of Biotechnology, Chosun University)
Publication Information
KSBB Journal / v.29, no.1, 2014 , pp. 29-35 More about this Journal
Abstract
In this study, antioxidant and antimicrobial activities of ethanolic crude extract and its five different solvent subfractions (namely, ethyl acetate fraction, n-butanol fraction, chloroform fraction, n-hexane fraction and the aqueous fraction) from a mixture of four different medicinal herbs (Galla rhois, Achyranthes japonica Nakai, Terminalia chebula Retz and Glycyrrhiza uralensis) were investigated. Among all the tested mixture combination of four medicinal herbs, 5:3:1:1 ratio of Galla:Achyranthes : Terminalia : Glycyrrhiza had the best antimicrobial effects against four strains of microorganisms (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli) and exhibited the highest DPPH radical-scavenging activity. Further sub-fractions with solvents were screened for antioxidant and antimicrobial activities. Antioxidant activity in order was ethyl acetate fraction > n-butanol fraction > chloroform fraction > nhexane fraction > aqueous fraction. The n-butanol extracted fraction showed the highest level of antimicrobial activity in com- parison to other fractions. In addition, all those fractions did not show any cytotoxicity against human skin cell CCD-986sk. These results suggest that 5:3:1:1 combination extracts of medicinal herbs (Galla : Achyranthes : Terminalia : Glycyrrhiza) may be potentially used as a safe natural antimicrobial preservative.
Keywords
Galla rhois; Achyranthes japonica Nakai; Terminalia chebula Retz; Glycyrrhiza uralensis; Antimicrobial; Antioxidant; Preservative;
Citations & Related Records
Times Cited By KSCI : 22  (Citation Analysis)
연도 인용수 순위
1 Lee, J. W., Y. J. Ji, M. H. Yu, H. G. Im, M. H. Hwangbo, and I. S. Lee (2005) Antimicrobial effect of extract of Glycyrrhiza uralensis on methicillin-resistant Staphylococcus aureus. Kor. J. Food Sci. Technol. 37: 456-464.   과학기술학회마을
2 Cai, H., S. I. Choi, Y. M. Lee, and T. R. Heo (2002) Antimicrobial effects of herbal medicine extracts on Staphylococcus aureus and Escherichia coli O157:H7. Kor. J. Biotechnol. Bioeng. 17: 537-542.   과학기술학회마을
3 Ahn, E. Y., D. H. Shin, N. I. Baek, and J. A. Oh (1998) Isolation and identification of antimicrobial active substance from Glycyrrhiza uralensis Fisch. Kor. J. Food Sci. Technol. 30: 680-687.   과학기술학회마을
4 Kim, S. J., J. Y. Shin, Y. M. Park, K. M. Chung, J. H. Lee, and D. H. Kweon (2006) Investigation of antimicrobial activity and stability of ethanol extracts of Licorice root (Glycyrrhiza glabra). Kor. J. Food SCI. Technol. 38: 241-248.   과학기술학회마을
5 Bae, J. S., H. S. Lee, H. Y. Lee, B. H. Yoo, T. W. Kim, Y. H. Kim, and T. H. Kim (2012) Evaluation of acetylcholinesterase inhibitory and antioxidative activities of Rhus javanica. Kor. J. Food Preserv. 19: 751-756.   과학기술학회마을   DOI   ScienceOn
6 Seo, H. S. (2010) Studies on the antimicrobial effect and antioxidative activity of fermented Galla Rhois Extract. Ph.D. Thesis. University of Joongbu, Kumsan-gum, Chung-nam, Korea.
7 Kim, M. S., J. S. Jeong, H. Y. Lee, Y. S. Ju, G. S. Bae, S. W. Seo, I. J. Cho, S. J. Park, and H. J. Song (2011) The anti-inflammatory effect of Achyranthes japonica on lipopolysaccharide-induced inflammatory activity in murine macrophages. Kor. J. Herbology. 26: 51-57.   과학기술학회마을
8 Lee, H. S., Y. C. Koo, and K. W. Lee (2009) Suppressive activity of extract of Termialia chebula Retz. on hepatic fibrosis. Kor. J. Food Sci. Technol. 41: 597-601.   과학기술학회마을
9 Im, S. J., N. Y. Lim, S. W. Lee, G. S. Kwak, S. H. Ahn, Y. J. Mun, and W. H. Woo (2003) Effect of Glycyrrhizae Radix water extract on the melanogenesis of human melanoma cell. Kor. J. Oriental Physiol. Pathol. 17: 368-373.   과학기술학회마을
10 Kim, M. J., T. K. Jung, I. G. Hong, and K. S. Yoon (2006) Comparison of anti-microbial oils as natural preservatives. J. Soc. Cosmet. Scientists Korea. 32: 99-103.
11 Lee, J. Y., J. N. Lee, G. T. Lee, and K. K. Lee (2012) Development of antimicrobial plant extracts and its application to cosmetics. J. Soc. Cosmet. Scientists Korea. 38: 171-179.   과학기술학회마을   DOI
12 Cho, E. M., J. T. Bae, H. B. Pyo, and G. S. Lee (2008) Antimicrobial plant extracts as an alternative of chemical preservative: Preservative efficacy of Terminalia chebula, Rhus japonica (gallut) and Cinnmomum cassia extract in the cosmetic formular. J. Soc. Cosmet. Scientists Korea. 34: 325-331.   과학기술학회마을
13 Park, S. M., K. A. Lee, M. Y. Yun, Y. J. Kim, and S. H. Lee (2011) Study of natural preservative system using the mixture of Scutellariae radix, Acacia nilotica and Citrus reticulata extracted from polyhydric alcohols. KSBB J. 26: 533-537.   과학기술학회마을   DOI   ScienceOn
14 Yang, H. G., H. J. Kim, H. S. Kim, and S. N. Park (2012) Antioxidative and antibacterial activities of Artemisia princeps Pampanini extracts. Kor. J. Microbiol. Biotechnol. 40: 250-260.   과학기술학회마을   DOI   ScienceOn
15 Kim, S. Y., D. H. Won, and S. N. Park (2010) Antibacterial activ-ity and component analysis of Persicaria perfoliata extracts. Kor. J. Microbiol. Biotechnol. 38: 278-282.   과학기술학회마을
16 Nam, S. H. and M. Y. Kang (2000) Screening of antioxidative activity of hot-water extracts from medicinal plants. J. Kor. Soc. Agric. Chem. Biotechnol. 43: 141-147.   과학기술학회마을
17 Kim, K. J., J. R. Do, J. H. Jo, Y. M. Kim, B. S. Kim, S. D. Lim, and S. N. Kang (2005) Antibacterial activity of Terminalia chebula Retz. extract against food spoilage microorganisms. Kor. J. Food Sci. Technol. 37: 498-503.   과학기술학회마을
18 Esposito, E., F. Bortolotti, C. Nastruzzi, E. Menegatti, and R. Cortesi (2003) Diffusion of preservatives from topical dosage forms: A comparative study. J. Cosmet. Sci. 54: 239-250.
19 Steinberg, D., Z. Hirschfeld, I. Tayeb, S. Ben-Yosef, A. David, and M. Friedman (1999) The effect of parabens in a mouthwash and incorporated into a sustained release varnish on salivary bacteria. J. Dent. 27: 101-106.   DOI   ScienceOn
20 Vilaplana, J. and C. Romaguera (2000) Contact dermatitis from parabens used as preservatives in eyedrops. Contact Dermatitis. 43: 248.
21 Choi, H. S., J. S. Kim, D. S. Jang, Y. B. Yu, Y. C. Kim, and J. S. Lee (2005) Antibacterial activities of Galla Rhois extracts against fish pathogenic bacteria. J. Fish Pathol. 18: 239-245.   과학기술학회마을
22 Jo, H. C., K. S. Han, and E. Y. An (2000) Gall formation on different age, habitat, and parasite position in Rhus javanica L.. Kor. J. Medicinal Crop Sci. 8: 304-311.   과학기술학회마을
23 Jung, S. M., S. I. Choi, S. M. Park, and T. R. Heo (2007) Antimicrobial effect of Achyranthes japonica Nakai extracts against Clostridium difficile. Kor. J. Food Sci. Technol. 39: 564-568.   과학기술학회마을
24 Park, J. S., N. S. Seong, and Y. J. Lee (2007) Comparative study on the anti-oxidative effects of Achyranthis japonicae Radix, Achyranthis bidentatae Radix and Cyathulae Radix. Kor. J. Herbology. 22: 155-167.   과학기술학회마을
25 Chae, I. G., M. H. Yu, H. I. Kim, and I. S. Lee (2011) Anti-inflammatory and anti-oxidative activity of methanol extract from Terminalia chebula Retz., Lavandula spica L., and Dalbergia odorifera T. in RAW 264.7 cells. J. Life Sci. 21: 561-567.   과학기술학회마을   DOI   ScienceOn
26 Han, S. B., H. A. Gu, S. J. Kim, H. J. Kim, S. S. Kwon, H. S. Kim, S. H. Jeon, J. P. Hwang, and S. N. Park (2013) Comparative study on antioxidative activity of Glycyrrhiza uralensis and Glycyrrhiza glabra extracts by country of origin. J. Soc. Cosmet. Scientists Korea. 39: 1-8.   과학기술학회마을   DOI
27 Kim, E. S., S. I. Jeong, J. H. Kim, C. Park, S. M. kim, J. K. Kim, K. M. Lee, S. H. Lee, H. So, and R. Park (2009) Synergistic effects of the combination of 20-hydroxyecdysone with ampicillin and gentamicin against methicillin-resistant Staphylococcus aureus. J. Microbiol. Biotechnol. 19: 1576-1581.   과학기술학회마을   DOI
28 Jang, S. J., G. D. Lee, J. S. Kim, and H. S. Yoon (1992) Antioxidative effectiveness of Terminalia chebula Retz extracts. Agric. Res. Bull. Kyungpook Natl. Univ. 10: 11-17.
29 Woo, K. S., K. I. Jang, K. Y. Kim, H. B. Lee, and H. S. Jeong (2006) Antioxidative activity of heat treated Licorice (Glycyrrhiza uralensis Fisch) extracts. Kor. J. Food Sci. Technol. 38: 355-360.   과학기술학회마을
30 Kim, S. J., D. H. Kweon, and J. H. Lee (2006) Investigation of antioxidative activity and stability of ethanol extracts of Licorice root (Glycyrrhiza glabra). Kor. J. Food Sci. Technol. 38: 584-588.   과학기술학회마을