• 한국균학회지
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• 제43권1호
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• pp.40-46
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• 2015

야생 꽃송이버섯의 생리활성 물질을 탐색하여 기능성 식품 개발에 이용하기 위해 꽃송이버섯 물 추출물과 유기용매 분획물의 혈전 용해 활성과 트롬빈 저해 활성, 항산화 활성 및 항염증 활성을 확인하였다. 부탄올 분획물과 에틸아세테이트 분획물이 각각 0.70 plasmin unit과 2.03 plasmin unit의 높은 혈전 용해 활성을 나타내고, 클로로포름 분획물이 83.87%의 높은 트롬빈 저해 활성을 나타냈으며, 에틸아세테이트 분획물이 95.94%의 높은 항산화 활성을 나타냈으며, 클로로포름 분획물은 82.62%의 높은 항염증 활성을 나타냈다. 그러므로 본 연구에서 나타난 꽃송이버섯의 혈전 용해, 트롬빈 저해, 항산화 및 항염증 효과들의 우수한 생리활성 결과들로부터 꽃송이버섯은 혈관계 질환 성인병 치료와 예방을 위한 기능성 식품소재로 활용 가치가 매우 큼을 알 수 있었다.

• 생약학회지
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• 제10권3호
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• pp.125-135
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• 1979

This study was undertaken to search for a new antihypertensive or hypotensive agents in natural crude products in Korea. Betula schmidtii Regel, a tree belonging to the Betulaceae family, is cultivated for the most part of our country, and their cortecies or tree milk has been used as folk remedy, which exerted good antiinflammatory effect on rheumatic fever, arthritis or rheumatism. The rats were treated with several extracts from Betula schmidtii Regel, that is, extracts of water, methanol from inner cortecies, extracts of ether, methanol, hexane, chloroform or butanol from outer and total cortecies. In this experiment, we measured the blood pressure and heart rate in anesthetized rats, and atropine, diphenhydramine, phentolamine, propranolol, epinephrine, hexamethonium and hydralazine were pretreated prior to Betula extracts to clarify the mechanism of the hypotensive action. The results of the experiment were as follows; 1. BOIE(Betula outer cortex water-insoluble extract) and BICE(Betula inner cortex chloroform extract) elevated the blood pressure and heart rate. 2. BIME(Betula inner cortex methanol extract), BIBE(Betula inner cortex butanol extract) and BIWE (Betula inner cortex water extract) significantly lowered the blood pressure and heart rate. 3. With the pretreatment of hydralazine, the hypotensive effect of Betula schmidtii Regel was significantly blocked. From the above results, it is suggested that the hypotensive effect of Betula schmidtii Regel is due to the direct vasodilation of blood vessel.

• 한국식품조리과학회지
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• 제14권5호
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• pp.498-502
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• 1998

천연식품보존제의 개발을 위해 우리나라 전지역에서 쉽게 구할 수 있고 산나물로 많이 이용되어온 질경이를 대상으로 식품부패미생물에 대한 항균효과를 검색하였다. 질경이 메탄을 추출물은 1.5mg/ml의 농도에서 곡류의 부패를 일으키는 B. subtilis와 호염균으로 식중독의 원인이 되는 V. parahaemolyticus의 성장을 완전히 억제하였다. 인공합성보존제인 Sodium propionate는 1%의 농도에서 B. subtilis는 80.97%, E.coli는 26.57%, S. aureus는 31.28%, L. monocytogenes는 72.71% 그리고 V. parahaemolyticus는 19.75% 저해되었으며 5% 농도에서는 5균주 모두 완전히 저해되었다. 이상의 결과로 볼 때 질경이의 메탄올추출물이 sodium propionate 보다 10배 낮은 저 농도에서 식품부패 미생물의 성장을 완전히 저해하는 것으로 나타났다. 또한 각 용매별로 분획하여 항균성을 살펴본 결과 ethylacetate 분획물이 다른 분획보다 높은 항균력을 보였는데 B. subtilis, E. coli와 V. parahaemolyticus는 1.0 mg/ml의 농도에서 성장이 저해되었다. 특히 chloroform 분획물은 S. aureus에 대하여 250$\mu\textrm{g}$/disc의 농도에서 강한 저해력을 보였다.

• 한국식품영양과학회지
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• 제30권6호
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• pp.1287-1292
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• 2001

본 연구는 옛부터 식용 및 약용으로 이용되고 있는 노루궁뎅이 버섯의 항돌연변이 및 암 예방 효과를 검색하기 위하여, 노루궁뎅이 버섯의 항돌연변이원성 및 quinone reductase (QR) 유도활성 효과를 살펴보았다. 노루궁뎅이 버섯의 돌연 변이 억제 효과를 Ames test로 검색한 결과, 노루궁뎅이 버섯의 메탄을 추출물과 분획물 그 자체의 돌연변이 원성은 없었으며, 노루궁뎅이 버섯의 메탄을 추출물은 직접변이원인 N- methyl- N'-nitro-guanidine(MNNG)와 간접변 이원인 B(a)P의 돌연변이 유발성을 크게 저해하였다. 또한 메탄올 추출물에서 분리한 분획물들도 유의성 있는 돌연변이 억제효과를 나타내었으며 특히 헥산, 클로르포름 및 에틸아세테이트 분획물의 경우는 부탄올과 물 분획 물보다 MNNG 와 13(a)P에 대해서 더 큰 항돌연변이 효과를 나타내었다. 그리고 노루궁뎅이 버섯에서 암 예방 효소계인 QR의 유도활성을 hepalclc7 세포주를 사용하여 검토한 결과에서는 노루 궁뎅이 버섯의 메탄을 추출묵과 헥산, 클로르포름 그리고 에틸아세테이트 분획물이 QR 효소활성을 유의적으로 증가시키는 것으로 나타났으며 나머시 분획물은 거의 효소활성에 영향을 미치지 않았다. 이상의 실험 결과로 노루궁뎅이 버섯은 발암물질로부터 생계를 보호하는 불질을 함유하고 있을 것으로 사료된다.

• Advances in Traditional Medicine
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• 제3권3호
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• pp.141-146
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• 2003

The methanol extracts of the aerial parts, leaves and stem of Hypericum hookerianum were tested for in vitro cytotoxicity on selected normal and cancer cell lines and anti tumor activity using DLA cells. Cell viability and morphological changes were assessed. Among the three extracts tested, the stem extract of Hypericum hookerianum showed potent cytotoxicity against HEp-2 and RD cell lines. The $CTC_{50}$(concentration required to reduce viability by 50%) of this extract was found to be $2.02\;{\mu}g/ml$ for RD cell line, $10.25\;{\mu}g/ml$ for HEp-2 cell line and $100.06\;{\mu}g/ml$ for Vero cell line. In the clonogenic assay, no colony formation was observed up to a concentration of $100\;{\mu}g/ml$. In the short term cytotoxicity studies using DLA cells, 50% viability was observed in the concentration range of $50-100\;{\mu}g/ml$ for aerial parts, $100-200\;{\mu}g/ml$ for stem and more than $200\;{\mu}g/ml$ for leaf extracts of Hypericum hookerianum. In the long-term activity using HEp-2 cell line, no colony formation was observed over a concentration of 200 mg/ml for the stem extract. Hypericum hookerianum stem extract was fractionated into petroleum ether, chloroform, ethyl acetate and methanol soluble fractions. The petroleum ether and chloroform soluble fractions showed higher cytotoxic activity against HEp-2 cell line when compared to the other two fractions. The methanol stem extract of Hypericum hookerianum has the potential for further investigation in animal models to determine its anti-tumor activity and to identify its active principles.

• 대한화장품학회지
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• 제16권1호
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• pp.40-46
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• 1990

A method was described for the analysis of Artemisia extract in cosmetic soap. Esculetin-6-methylether was used as indicator ingredient for analysis of Artemisia extract. It was isolated from the plant and purified with silicagel column. The structure was conformed with IR, NMR and MASS spectroscopy. The Artemisia extract in sample was isolated with chloroform and interfering com- pounds were eliminated with silicagel column. The Artemisia extract was determined by reverse phase high- performance liquid chromatography with a fluorescence detector and a solvent system of H2O/THF/MeOH. The recorveries of Artemisia extract were 93.9-106.1% in the cosmetic soap.

• 대한수의사회지
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• 제13권6호
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• pp.393-395
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• 1977

Ranunculus species, a poisonous plant that grow in the field and the dales throughout the country has been recommended in folklore for the therapy of traumatic inflammation. A chloroform extract of this plant has been shown to inhibit the growth of severa

• 생약학회지
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• 제11권2호
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• pp.104-107
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• 1980

Mansam, the root of Codonopsis pilosula (Campanulaceae) has a action on blood metabolism. It has been known to possess anti leukemic effect by increasing of red blood cell, at same time, by decreasing white blood cell, with this connection, present study is aimed to clarify such potential anti leukenic effect by testing ethanol extract and chloroform extract against murine tumor model, lymphocytic leukemia P388. The data indicated that these extracts appeared to be inactive against this tumor line.

• 한국미생물·생명공학회지
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• 제21권6호
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• pp.628-634
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• 1993

The inhibitory effects of various extracts from Chinese pepper on the mutagenicity and the growth of MG-63 human osteosarcoma cells were studied. Chinese pepper was extracted with methanol and then the methanol extract was further fractionated by using hexane, chloroform, ethyl acetate and butanol. The methanol extract of Chinese pepper revealed the strong antimutagenic activity on the aflatoxin B1(AFB1) and N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) in Ames mutagenicity test and SOS chromotest.

• Archives of Pharmacal Research
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• 제25권1호
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• pp.61-66
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• 2002

From our previous result that Panax ginseng head extract had inhibition of gastric damages, the extract was fractionated. Among the hexane, chloroform, butanol and water fractions, butanol fraction Showed the most potent inhibition of HCl.ethanol-induced gastric lesion, aspirin-induced gastric ulcer, acetic acid-induced ulcer and Shay ulcer. Butanol fraction showed significant increase in mucin secretion, and inhibited malondialdehyde (MDA) and $H^{+}/K^{+}ATPase$ activity in the stomach. This results indicate that the effectiveness of the fraction on gastric damages might be related to inhibition of acid secretion, increment of mucin secretion and antioxidant property.