• Genomics & Informatics
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• v.8 no.1
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• pp.50-57
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• 2010

Mercuric chloride, a model nephrotoxicant was used to elucidate time- and dose- dependent global gene expression changes associated with proximal tubular toxicity. Rat kidney cell lines NRK-52E cells were exposed for 2, 6 and 12 hours and with 3 different doses of mercuric chloride. Cell viability assay showed that mercuric chloride had toxic effects on NRK-52E cells causing 20% cell death (IC20) at $40{\mu}M$ concentration. We set this IC20 as high dose concentration and 1/5 and 1/25 concentration of LC20 were used as mid and low concentration, respectively. Analyses of microarray data revealed that 738 genes were differentially expressed (more than two-fold change and p<0.05) by low concentration of mercuric chloride at least one time point in NRK-52E cells. 317 and 2,499 genes were differentially expressed at mid and high concentration of mercuric chloride, respectively. These deregulated genes showed a primary involvement with protein trafficking (CAV2, CANX, CORO1B), detoxification (GSTs) and immunity and defense (HMOX1, NQO1). Several of these genes were previously reported to be up-regulated in proximal tubule cells treated with nephrotoxicants and might be aid in promoting the predictive biomarkers for nephrotoxicity.

• Food Science of Animal Resources
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• v.33 no.3
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• pp.395-402
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• 2013

Sodium chloride is used to improve various properties of processed meat products, e.g., taste, preservation, water binding capacity, texture, meat batter viscosity, safety, and flavor; however, many studies have shown that sodium chloride increases the resistance of many foodborne pathogens to heat and acid. Listeria monocytogenes has been isolated from various readyto- eat (RTE) meat and dairy products formulated with sodium chloride; therefore, the objective of this paper was to review the effects of sodium chloride on the physiological characteristics of L. monocytogenes. The exposure of L. monocytogenes to sodium chloride may increase biofilm formation on foods or food contact surfaces, virulence gene transcription, invasion of Caco-2 cells, and bacteriocin production, depending on L. monocytogenes strain and serotype as well as sodium chloride concentration. When L. monocytogenes cells were exposed to sodium chloride, their resistance to UV-C irradiation and freezing temperatures increased, but sodium chloride had no effect on their resistance to gamma irradiation. The morphological properties of L. monocytogenes, especially cell elongation and filament formation, also change in response to sodium chloride. These findings indicate that sodium chloride affects various physiological responses of L. monocytogenes and thus, the effect of sodium chloride on L. monocytogenes in RTE meat and dairy products needs to be considered with respect to food safety. Moreover, further studies of microbial risk assessment should be conducted to suggest an appropriate sodium chloride concentration in animal origin foods.

• Journal of Korean Society of Water and Wastewater
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• v.18 no.5
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• pp.580-587
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• 2004

The objective of this research is to investigate the electrochemical oxidation process for nitrogen removal in wastewater involving chloride ion and nitrogen compounds. The process experiment of electrochemical oxidation was conducted by using the stainless steel tube type reactor and the $Ti/IrO_2$ as anode. Free chlorine production and current efficiency variation for total nitrogen removal was compared depending on whether electrolyte is added, and the nitrogen type distribution under an operating condition. When chloride was added as electrolyte, it was found that production of free chlorine increased and the concentration of the chloride decreased as retention time passed. The concentration of chloride in influent decreased from 1,660 to 1,198 mg/L at the current density of $6.7A/dm^2$, while concentration of free chlorine increased to 132 mg/L. Current efficiency in removal of ammonium nitrogen was increased when chloride was dosed as electrolyte. It was observed that ammonium nitrogen was oxidized to nitrite and nitrate through electrochemical oxidation and that the concentration of total nitrogen in influent was reduced from 22.58 to 4.00 mg/L at the short retention time of 168 seconds through the electrochemical oxidation of nitrogen.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.574-578
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• 2005

The purpose of the study is to research the proper conditions to prepare the calcium-alginate microspheres using a emulsion method. The calcium-alginate microspheres were prepared at distinct concentrations (alginate; 1%, 1.5%, 2% (w/v), calcium chloride; 2%, 4%, 8%, 10%(w/v)). The shape of the microspheres prepared was spherical. With increasing alginate and calcium chloride concentration the mean size of the microspheres decreased gradually. In release test, the amount of ovalbumin released from the calcium-alginate mcirospheres was decreased by the increasing of alginate and calcium chloride concentration. In this study the best result was obtained at a 2% of alginate concentration and 10% of calcium chloride concentration.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.05a
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• pp.705-710
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• 2003

In this study, to confirm the diffusion coefficient of chloride ion is affected by the concentration of NaCl solution, capacity of voltage, time of an electric current, the diffusion coefficient of chloride ion in concrete was investigated through an electrochemical accelerated test. and the results of these test were compared with the diffusion coefficient of chloride ion by test of sodium chloride solution digestion. As the results of this study, the diffusion coefficient of chloride ion wasn't affected by the concentration of NaCl solution, capacity of voltage, time of an electric current within the range of this study and was similar to the diffusion coefficient of chloride ion by test of sodium chloride solution digestion.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.15 no.2
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• pp.71-79
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• 2003

The estimation functions were proposed for calculating diffusion coefficient, chloride binding, and evaporable water. The program estimating chloride ion penetration was developed on the basis of these functions and the effects of humidity, curing temperature, water-cement ratio, and $C_3$A on chloride penetration were analyzed. The relative humidity increases the depth of chloride ion penetration and the trend becomes greater with aging. On the contrary, the influence of curing temperature on chloride ion penetration decreases with aging. By the way, the rise of $C_3$A in cement increases total chloride concentration on the surface as the bound chloride concentration increases but it decreases total chloride concentration on the inner part as the diffusion velocity of free chloride decreases. The fall of water-cement ratio decreases the chloride penetration depth rapidly. Therefore, the reduction of water-cement ratio may be the most effective method for reducing of the steel corrosion by chloride penetration.

• Safety and Health at Work
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• v.1 no.1
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• pp.98-101
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• 2010

This study explored the health hazard of those exposed to methylene chloride by assessing its atmospheric concentration when a tear gas mixture was aerially dispersed. The concentration of methylene chloride ranged from 311.1-980.3 ppm (geometric mean, 555.8 ppm), 30 seconds after the dispersion started. However, the concentration fell rapidly to below 10 ppm after dispersion was completed. The concentration during the dispersion did not surpass the National Institute for Occupational Safety and Health 'immediately dangerous to life or health' value of 2,300 ppm, but did exceed the American Conference of Governmental Industrial Hygienists excursion limit of 250 ppm. Since methylene chloride is highly volatile (vapor pressure, 349 mmHg at $20^{\circ}C$), the post-dispersion atmospheric concentration can rise instantaneously. Moreover, the o-chlorobenzylidenemalononitrile formulation of tear gas (CS gas) is an acute upper respiratory tract irritant. Therefore, tear gas mixtures should be handled with delicate care.

• Corrosion Science and Technology
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• v.21 no.4
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• pp.300-313
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• 2022

Interest in polymer electrolyte fuel cell is growing to replace fossil fuels. In particular, in order to reduce the cost and volume of the fuel cell, research on a metallic bipolar plate is being actively conducted. In this research, investigated the effects of temperature and chloride concentration on the electrochemical characteristics and damage behavior of 316L stainless steel in an accelerated solution simulating the cathodic operating condition of PEMFC(Polymer electrolyte membrane fuel cell). As a result of the experiments, the corrosion current density, damage size, and surface roughness increased as the temperature and chloride concentration increased. In particular, the temperature had a significant effect on the stability of the oxide film of 316L stainless steel. In addition, it was described that the growth of the pit was affected by the chloride concentration rather than the temperature. As a result of calculating the corrosion tendency to compare the pitting corrosion rate and the uniform corrosion rate, the uniform corrosion tendency became larger as the temperature increased. And the effects of chloride concentration on corrosion tendency was different according to temperature.

• Geotechnical Engineering
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• v.13 no.2
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• pp.17-28
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• 1997

In this study sodium chloride solution is employed for chemicals, and several cylindrical triaxial tests are performed on the sand-bentonite mixtures saturated with sodium chloride solution. Deformation(elastic modulus, E) and strength(cohesion, c', and angle of friction, f') parameters are obtained from the triaxial tests as functions of confining pressure and sodium chloride solution concentrations. The results here indicate an increase in the value of effective cohesion with increase in the concentration of NaCl solution, which can be explained by using the Gouy-Chapman model. The value of the effective angle of shearing resistance does not show significant change with the increase in concentration of NaCl solution. The Young's modulus also increases with the increase in concentration of NaCl solution.

• Journal of Korean Society for Atmospheric Environment
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• v.2 no.3
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• pp.27-33
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• 1986

Atmospheric particulate matter (A. P. M.) was collected and size-fractionated by an Andersen high-volume air sampler over 15 month period from Jan. 1985 to Feb. 1986 in Seoul. The concentration of chloride, nitrate and sulfate were extracted in an ultrasonic bath and were analyzed by ion chromatography. The annual arithmetical mean of A. P. M. was 128.54 $\mug/m^3$. The concentration of anions were 2.88 $\mug/m^3$ for chloride, 3.86$\mug/m^3$ for nitrate, and 25.44$\mug/m^3$ for sulfate. The content of A. P. M. was lowest in the particle size range 1.1 $\sim 3.3\mum$ and increased as the particle size increased or decreased. And the anions exhibited a seasonal variation in the isize distribution. The contents of anions were higher in winter than summer. Ther ratio of fine particles to the total particles defined by F/T for chloride, nitrate and sulfate. The F\ulcornerT of these anion generally decrease with increasing air temperature. This tendency was prevalent in the chloride and nitrate.