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http://dx.doi.org/10.5808/GI.2010.8.1.050

Effects of Mercuric Chloride on Gene Expression in NRK-52E Cells  

Ahn, Joon-Ik (Pharmacological Research Division, Toxicological Evaluation Research Department, National Institute of Food and Drug Safety Evaluation)
Baik, Si-Yeon (Pharmacological Research Division, Toxicological Evaluation Research Department, National Institute of Food and Drug Safety Evaluation)
Ko, Moon-Jeong (Pharmacological Research Division, Toxicological Evaluation Research Department, National Institute of Food and Drug Safety Evaluation)
Shin, Hee-Jung (Pharmacological Research Division, Toxicological Evaluation Research Department, National Institute of Food and Drug Safety Evaluation)
Chung, Hye-Joo (Pharmacological Research Division, Toxicological Evaluation Research Department, National Institute of Food and Drug Safety Evaluation)
Jeong, Ho-Sang (Pharmacological Research Division, Toxicological Evaluation Research Department, National Institute of Food and Drug Safety Evaluation)
Publication Information
Genomics & Informatics / v.8, no.1, 2010 , pp. 50-57 More about this Journal
Abstract
Mercuric chloride, a model nephrotoxicant was used to elucidate time- and dose- dependent global gene expression changes associated with proximal tubular toxicity. Rat kidney cell lines NRK-52E cells were exposed for 2, 6 and 12 hours and with 3 different doses of mercuric chloride. Cell viability assay showed that mercuric chloride had toxic effects on NRK-52E cells causing 20% cell death (IC20) at $40{\mu}M$ concentration. We set this IC20 as high dose concentration and 1/5 and 1/25 concentration of LC20 were used as mid and low concentration, respectively. Analyses of microarray data revealed that 738 genes were differentially expressed (more than two-fold change and p<0.05) by low concentration of mercuric chloride at least one time point in NRK-52E cells. 317 and 2,499 genes were differentially expressed at mid and high concentration of mercuric chloride, respectively. These deregulated genes showed a primary involvement with protein trafficking (CAV2, CANX, CORO1B), detoxification (GSTs) and immunity and defense (HMOX1, NQO1). Several of these genes were previously reported to be up-regulated in proximal tubule cells treated with nephrotoxicants and might be aid in promoting the predictive biomarkers for nephrotoxicity.
Keywords
gene expression; mercuric chloride; nephrotoxicity; NRK-52E cells;
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