• 한국연소학회지
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• 제15권4호
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• pp.37-42
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• 2010

The measurement of heat release rate is of great importance in the study of thermo-acoustic instability occurring in lean premixed combustion and the chemiluminescence emission has been used as an indicator of heat release in combustion instability studies primarily for its relative simplicity. This paper presents results of experimental study of flame chemiluminescence from an atmospheric, swirl-stabilized, turbulent lean premixed flame with a main emphasis on the effect of $CO_2^*$ background level in the $CH^*$ and $OH^*$ band. The test results show that the effect of $CO_2^*$ level in the $CH^*$ band is greater than that in the $OH^*$ band. Also, the background to peak ratio for both $CH^*$ and $OH^*$ bands can be expressed as a function of equivalence ratio, almost regardless of a change in the inlet velocity.

• 한국물환경학회지
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• 제25권1호
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• pp.84-89
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• 2009

Our nation's water resources remain susceptible to contamination by phenolic agrichemicals. These compounds can be toxic to a variety of organisms including humans. Their disposal is restricted in many countries with strict limits for acceptable concentrations in drinking water. Enzyme-mediated in situ stabilization has been advocated as an approach for the treatment of phenolic compounds in soils and groundwater. This study reports the development of a new approach to quantify the activity of the HRP enzyme in aqueous systems. The method is based on the coupled processes of energy transfer and enhanced chemiluminescence using a luminol-$H_2O_2$-HRP system. In this study, the effects of solution pH, ionic strength and aqueous concentrations of HRP, $H_2O_2$ and enhancer were evaluated on the p-iodophenol-enhanced, HRP-catalyzed chemiluminescence reaction intensity in Tris-HCl buffer. All assay components were found to affect the maximum chemiluminescene intensity. The calibration curve for HRP showed the linear relationship with maximum light intensity.

• Bulletin of the Korean Chemical Society
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• 제32권7호
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• pp.2187-2192
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• 2011

Hydrogen peroxide plays a key role as a second messenger in the normal cellular signaling but its overproduction has been implicated in various life-threatening diseases. Peroxalate chemiluminescence is the light emission from a three component reaction between peroxalate, hydrogen peroxide and fluorophores. It has proven great potential as a methodology to detect hydrogen peroxide in physiological environments because of its excellent sensitivity and specificity to hydrogen peroxide. We developed chemiluminescent micelles composed of amphiphilic polymers, peroxalate and fluorescent dyes to detect hydrogen peroxide at physiological concentrations. In this work, we studied the relationship between the chemiluminescence reactivity and stability of peroxalate by varying the substitutes on the aryl rings of peroxalate. Alkyl substitutes on the aryl ring of peroxalate increased the stability against water hydrolysis, but diminished the reactivity to hydrogen peroxide. Chemiluminescent micelles encapsulating diphenyl peroxalate showed significantly higher chemiluminescence intensity than the counterpart encapsulating dimethylphenyl or dipropylphenyl peroxalate. Diphenyl peroxalate-encapsulated micelles could detect hydrogen peroxide generated from macrophage cells stimulated by lipopolysaccharide (LPS) and image hydrogen peroxide generated during LPS-induced inflammatory responses in a mouse.

• Journal of Photoscience
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• 제12권3호
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• pp.137-141
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• 2005

The chemiluminescence reaction of lophine with $H_2O_2$ in alkaline solution has been investigated for use in determination of $Cu^{2+}$ ions. The observed chemiluminescence intensity is found to be a function of the concentration of $Cu^{2+}$. Under the optimum reagent concentrations such as $4{\times}10^{-4}M$ lophine, 0.8 M KOH, 0.2M $H_2O_2,{\lambda}_{em}$, 533nm, the linear range and the detection limit were found to be 0.048ug/ml-48.32ug/ml (R=0.99897) and 0.005ulg/ml respectively. Relative standard deviation for five determinations of 24.16ug/ml $Cu^{2+}$ is 2.35%. The interference from other species was investigated. The proposed method was applied to the determination of $Cu^{2+}$ in different water samples.

• Bulletin of the Korean Chemical Society
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• 제33권1호
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• pp.171-176
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• 2012

A novel chemiluminescence (CL) system was established for the determination of cytosine arabinoside (Ara-C) in pharmaceutical preparations. It was showed that a clear CL signal was observed when Eosin Y mixed with Fenton reagent. The CL intensity was decreased significantly when Ara-C was added to the reaction system and partially scavenged the hydroxyl radicals in the solution. The extent of decrease in the CL intensity had a good stoichiometrical relationship with the Ara-C concentration. Based on this, we developed a new method for the determination of Ara-C using a flow injection analysis (FIA) technique with CL detection. Under the optimal conditions, the linear range of Ara-C concentration was $6.0{\times}10^{-9}\sim1.0{\times}10^{-7}mol/L$ (R = 0.9982) with a detection limit of $7.6{\times}10^{-10}mol/L$ (S/N=3), the RSD was 5.6% for $6.0{\times}10^{-8}mol/L$ Ara-C (n = 11). The method was successfully applied to the determination of Ara-C in injection samples. The possible chemiluminescence reaction mechanism was discussed.

• Nuclear Engineering and Technology
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• 제33권6호
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• pp.626-637
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• 2001

A series of study on energy dependence in chemiluminescence dosimetry with sugar and sorbite produced in two different countries was carried out administering a dose of 5 Gy to the samples at six different mean photon energies of 30, 50, 80, 130, 662 and 1250 keV. The results revealed distinct energy dependence of chemiluminescence(CL) output of sugar and sorbite. Although the energy dependence, in general, could be fitted by a polynomial of (os E, with I being radiation energy, up to cubic term, we reached a conclusion that the adoption of a fitting function, $y_{R}$$^{=a}$.(1-e $^{-b.logE}$)$^{c}$ +d, deduced from theoretical energy response curve calculated as the ratio of the mass energy absorption of the samples of interest to the soft tissue is more reasonable and rational. Herecoefficients $y_{R}$ is CL intensity, and a, b, c and d are constants to be determined in the fitting process. Energy dependence of relative sensitivities of one sample to the other, discrepancy in sensitivities of the samples from the two countries, and prominent grain size effect in Sorbitol were also shown.shown.

• 한국추진공학회지
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• 제22권1호
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• pp.72-79
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• 2018

초음파 진동자에 의해 미립화된 케로신 분무연소의 OH 라디칼과 CH 라디칼의 자발광 특성을 고찰하기 위한 실험이 수행되었다. ICCD 카메라를 이용하여 분무화염의 자발광 강도를 측정하였으며, 연소 시 소모된 연료량은 정밀유량측정법으로 계측하였다. 그 결과, 연료소모율은 수송기체인 공기 공급유량에 선형적으로 증가하였으며, 분무연소의 특징인 전형적인 그룹 연소가 관찰되었다. OH 라디칼과 CH 라디칼을 분석한 결과, 분사방향으로의 유량 증가에 따라 라디칼 방사강도의 최댓값은 감소하고 그 위치는 후류로 이동하여 반응대의 폭은 증가하였다.

• 응용화학
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• 제15권1호
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• pp.33-36
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• 2011

A chemiluminescent method with gold nanoparticles for determination of vitamin C has been presented. Gold nanoparticles highly increased chemiluminescence intensity of lumimol-H₂O₂ system by its catalytic effect. The gold nanoparticles enhanced chemiluminescent method is applicable for the determination of an antioxidant such as vitamin C. When vitamin C was introduced to the lumimol system with gold nanoparticles, chemiluminescence intensity was reduced with the concentration of the added vitamin C. Low concentrated vitamin C solutions in the range of 1×10^-3-1×10^-9 M were produced reduced chemiluminescence intensity of lumimol-H₂O₂ with gold nanoparticles system.

• 한국추진공학회지
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• 제18권1호
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• pp.73-84
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• 2014

본 논문에서는 화학발광 계측을 통한 연소화염의 당량비, 열방출율과 같은 물리량 판단에 대한 최신 연구결과를 정리 분석하였다. 현대의 연소장치는 연소의 동적 안정성 증대 및 공해물질 배출억제를 위해 세밀한 제어가 필요하다. 화학발광 세기 계측을 통한 물리량 파악은 그 적용에 있어서 많은 상대적 장점을 지니고 있다. 그러나 본 방법은 당량비, 연소압력, 입구온도, 난류세기, 연료 종류 등에 의해 많은 영향을 받는다. 연구결과를 종합하면, 메탄/공기 예혼합 화염에서 $CH^*/OH^*$가 연료 과농 조건을 제외하고 당량비와 밀접한 관계가 있다. 또한 넓은 공간에서 측정된 $OH^*$, $CH^*$ 신호는 전체 열방출율과 비례관계를 갖는 것으로 판단된다.

• 대한기계학회논문집B
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• 제39권6호
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• pp.485-496
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• 2015

본 논문은 와류 유동이 존재하는 케로신/공기 화염 자발광 특성을 실험적 접근방법으로 수행한 연구이다. 한국형 발사체 액체로켓엔진에 적용되는 Jet A-1 을 사용하였고, 와류 세기 영향을 파악하기 위해 세 가지의 스월러를 적용해 실험을 진행하였다. 와류 세기, 연소 공기온도 변화에 따른 화염 자발광을 분광기를 활용하여 계측하였다. 자발광 스펙트럼에서 $OH^*$, $CH^*$, $C_2{^*}$등의 라디칼에 의한 화학발광 특성을 파악하였다. 케로신 화염의 화학발광 세기는 와류 세기에 민감한 반응을 보였으며, 연소 공기온도에 의한 영향은 적게 받았다. 특히 $C_2{^*}$ 화학발광 방출 세기는 와류 세기와 당량비 변화에 민감하게 반응하였다. 화염 특성을 파악하기 위해 각 라디칼 세기 비로 데이터를 분석한 결과, $I_{OH^*}/I_{CH^*}$ 화학발광 세기 비는 공기 유량 변화에 의한 당량비 변화를 지시하기가 적합하며, $I_{C_2{^*}}/I_{CH^*}$ 화학발광 세기 비는 연료 유량변화에 따른 당량비 변화를 지시하기에 적합하였다.