• Journal of Applied Biological Chemistry
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• 제58권2호
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• pp.153-155
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• 2015

뽕나무(Morus alba L.) 뿌리껍질을 실온에서 80% MeOH 수용액으로 추출하고 이 추출물을 EtOAC, n-BuOH, 그리고 $H_2O$ 분획으로 나누었다. EtOAc 및 n-BuOH 분획에 대하여 silica gel, ODS 및 Sephadex LH-20 column chromatography를 반복 실시하여 4종의 phenolic 화합물을 분리, 정제하였다. NMR, IR, 및 EI/MS 등을 해석하여, norartocarpanone (1), 2',4',7-trihydroxy-(2S)-flavanone (2), methyl-${\beta}$-resorcylate (3), 그리고 (Z)-oxyresveratrol-4-O-${\beta}$-$\small{D}$-glucopyranoside (4)로 각각 구조동정하였다. 화합물 (Z)-oxyresveratrol-4-O-${\beta}$-$\small{D}$-glucopyranoside (4)는 상백피로부터는 이번 실험에서 처음으로 분리되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1568-1579
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• 2001

This study is aimed at evaluating the effect of Aspergillus oryzae fermentation extract (AFE) on corn silage fermentation characteristics. Trial included two groups of treatments, with or without AFE inclusion in corn ensilage. Sixty corn silage containers, including two treatments with thirty replicates each, were processed in a laboratory scale mini-silo of 21 cm radius by 45 cm height. Three replicate containers were opened and sampled for analysis at 0, 0.5, 1, 2, 3, 4, 6, 10, 18 and 34 days after being ensiled. One silage container from each treatment was installed with a remote controlled electronic thermometer to record the temperature changes. Analysis included silage temperature, pH, fermentation acids, the water-soluble carbohydrates and chemical compositions and the silage protein fractions. Results showed that on the first day, the temperature of the ensiled corn was slightly higher than room temperature, but returned to room temperature on the second day. The pH and concentrations of WSC, ADF, lignin and acetic acid in the AFE treated silage were significantly lower than the control groups (p<0.05). The lactic acid and crude protein on the other hand were significantly higher in the AFE treated silage as compared to the control (p<0.05) at the end of the ensilage period. The DM content was significantly higher (p<0.05) whereas the butyric acid content of the AFE treated silage was significantly lower (p<0.05) than the control at the end of the 34 day ensilage period. Titratable acid and buffering capacity in the corn silage were not significantly different between treatment groups (p>0.05). Ammonia N concentration in the AFE treated silage showed a trend of decrease (p>0.05). NPN and the protein fraction A in both groups increased during the conservation period, but fraction A in the AFE treated corn silage was significantly higher than the control silage (p<0.05). During the conservation period, the AFE treated corn silage showed a trend toward a decrease in fractions $B_1$, $B_3$ and C (p<0.05). The protein fraction B2 showed a trend toward increase in the control group and an inconsistent trend in the AFE treated silage during the ensiling period. The AFE treated silage showed a better Flieg score over the control silage (97 vs. 75) as calculated from the concentrations of lactic acid, acetic acid and butyric acid.

• Journal of Applied Biological Chemistry
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• 제59권4호
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• pp.357-360
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• 2016

국화(C. morifolium)의 꽃을 80 % MeOH 수용액으로 추출한 뒤, 감압농축한 추출물을 EtOAc, n-butyl alcohol 및 물분획으로 나누었다. EtOAc 분획과 n-butyl alcohol 분획에 대하여 $SiO_2$ 및 ODS column chromatography를 반복 실시하여 4종의 flavonoid를 분리, 정제하였다. Nuclear magnetic resonance, infrared spectrometry 및 FAB-MS data를 해석하여, 화합물 1-4를 각각 apigenin, acacetin, cosmetin (apigenin-7-O-${\beta}$-$\small{D}$-glucopyranoside), acacetin-7-O-${\beta}$-$\small{D}$-glucoside으로 구조 동정 하였다.

• Applied Biological Chemistry
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• 제50권3호
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• pp.233-237
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• 2007

순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH 추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, $H_{2}O$분획으로 나누었으며, EtOAc분획과 n-BuOH분획에 대해 silica gel 및 ODS column chromatography를 실시하여 5종의 화합물을 분리 정제하였다. $^{1}H-NMR$, $^{13}C-NMR$, DEPT spectrum 및 Mass spectrum 등을 통하여 palmitic acid methyl ester(1), linolenic acid methyl ester(2), linoleic acid methyl ester(3), ${\beta}-sitosterol$(4), daucosterol(5)으로 구조를 결정하였다.

• Journal of Applied Biological Chemistry
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• 제56권4호
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• pp.213-217
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• 2013

Phytochemical studies on the leaves of Ailanthus altissima (Simaroubaceae) have not been reported previously. Thus, the authors isolated and identified secondary metabolites from A. altissima. Dried and powdered leaves were extracted with 80% aqueous methanol, and the concentrated extract was successively partitioned with ethyl acetate, n-butanol, and water. Four flavonoids were isolated from the ethyl acetate fraction through repeated silica gel and octadecyl silica gel column chromatography. Spectroscopic data including NMR, MS, and IR allowed for identification of the chemical structures as quercetin (1), afzelin (2), quercitrin (3), and isoquercitrin (4). This is the first report of the isolation of these compounds from A. altissima. The four isolated flavonoids 1-4 as well as solvent fractions (ethyl acetate, n-butanol, and water), were evaluated for DPPH radical scavenging activity.

• 대한본초학회지
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• 제21권2호
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• pp.1-7
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• 2006

Objectives : The purpose of this study is to isolate tyrosinase inhibitory material from Pinellia ternata and characterize its own structure and activity. Methods : Pinellia ternata (600g) was extracted with 95% methanol (1L) at $37^{\circ}C$ for 4 days, with shaking at 250rpm. The extract was further solvent-fractionated with n-hexane, chloroform, ethylacetate and water. The active fraction was subjected to JAI recycling prep-HPLC JAIGEL GS-320 column. The structure was identified for the active peak with NMR and GC. Results : Tyrosinase was potently inhibited by 95% methanol extracts from Pinellia ternata. The $IC_{50}$ value of the extracts was estimated to be 0.05mg/ml. The extracts was divided into four solvent-fractions, and the most potent tyrosinase inhibition was found in ethylacetate layer. $IC_{50}$ value of ethylacetate fraction was 0.001mg/ml. This fraction was further purified with JAI Recycling Preparative HPLC (Model: LC 9104). The isolated compound showing inhibitory activity was characterized on its chemical structure by NMR and the compound was identified as 3,4-dihydroxybenzaldehyde. $IC_{50}$ was found to be 7.74 ${\mu}M$ which is much lower than that of kojic acid $(66.5{\mu}M)$. Conclusions : The data suggest that 3,4-dihydroxybenzaldehyde isolated and identified from Pinellia ternata is very strong inhibitor to melanin biosynthesis.

• Journal of Plant Biotechnology
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• 제36권1호
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• pp.64-67
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• 2009

순무 뿌리로부터 활성 물질을 분리 동정하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH 추출물을 얻었다. 이를 EtOAc 분획, n-BuOH 분획, $H_2O$ 분획으로 나누었으며, EtOAc분획과 n-BuOH 분획에 대해 silica gel 및 ODS column chromatography를 실시하여 4종의 이차대사산물을 분리 정제하였다. $^1H-NMR,\;^{13}C-NMR$, DEPT spectrum 및 mass spectrum등을 통하여 4- (methoxymethyl)phonol(1), ${\alpha}$- methoxy-2, 5- furandimethanel (2), phenyl-${\beta}$-glucopyranoside(3) 및 2-phenylethyl-${\beta}$-D -glucopyranoside (4)로 구조를 결정하였다. 이 화합물들은 순무에서는 처음 분리되었다.

• 한국응용과학기술학회지
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• 제31권4호
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• pp.740-747
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• 2014

식품부패미생물에 대한 정향추출물의 항진균력을 확인하고 유효성분을 분리한 후 그 구조를 확인하였다. 정향 추출물은 대표적인 항진균제인 메타중아황산칼륨 및 myconazole과 비슷한 항진균 활성을 나타내었다. 용매분획을 실시한 결과 hexane fraction이 가장 항진균활성이 높은 것으로 나타났다. 이 hexane 분획을 silica gel column chromatography를 통해 분리하여 항진균활성이 가장 큰 분획물인 JH-1, JH-2-1 및 JH-2-2을 분리하였다. 이들 화합물은 EI-MS 와 $^1H$-NMR, $^{13}C$-NMR 결과로부터 활성물질의 구조를 동정하였으며 그 결과 JH-1은 eugenol, JH-2-1은 eugenol acetate 그리고 JH-2-2은 chavicol인 것으로 확인하였다.

• Archives of Pharmacal Research
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• 제29권11호
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• pp.937-941
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• 2006

Ixeris dentata forma albiflora was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_{2}O$. Eight sesquiterpenes were isolated through repeated silica gel and octadecyl silica gel ($C_{18},\;ODS$) column chromatography of the EtOAc and n-BuOH fractions. Physicochemical analysis using NMR, MS and IR revealed the chemical structures of the sesquiterpenes, which were zaluzanin (1), 9a-hydroxyguaian-4(15), 10(14), 11 (13)-triene-6, 12-olide(2), $3{\beta}-O-{\beta}-D-glucopyranosyl-8{\beta}-hydroxyguaian$-4(15), 10(14)-diene-6, 12-olide (3), $3-O-{\beta}-D-glucopyranosyl-8{\beta}-hydroxyguauan$-10(14)-ene-6, 12-olide (4), ixerin M (5), glucozaluzanin C (6), crepiside I (7), and ixerin D (8). This is the first time that these sesquiterpene lactones have been isolated from this plant. Compounds 1, 2 and 7 revealed relatively high cytotoxicities on human colon carcinoma cell and lung adeno-carcinoma cell, while compounds 5 and 7 showed acyl-CoA: cholesterol acyltransferase (ACAT) inhibitory activity.

• 한국가축번식학회지
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• 제26권2호
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• pp.165-171
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• 2002

난포액에는 난자의 성숙을 억제하는 인자를 함유하고 있으나 화학적 성질이 정확히 알려져 있지않다. 본 연구에서는 1차 난모세포에서 난구세포의 해리와 제1극체의 형성을 억제하는 난포액 성분을 화학적으로 동정하기 위하여 수행되었다 이를 위하여 돼지 난포액을 methanol로 추출한 다음이 추출물을 Superose 12 및 Superdex column을 이용 연속적으로 분리하였으며, Superdex 분절은 PITC로 처리한 다음 아미노산 분석용 column을 이용 분석하였다. 얻어진 결과는 다음과 같다. 난포액은 1차 난모세포에서 난구세포의 해리와 제1극체의 형성을 억제하였다 난포액에서 추출 분리한 Superdex분절 RV2.11 역시 난구세포의 해리와 제1극체의 형성을 억제하였다. 아미노산 분석 결과, 분절 RV2.11은 proline으로 추정되며, proline은 난구세포의 해리와 제1극체의 형성을 억제하였다. 결론적으로 난포액에는 난구세포의 해리와 제1극체의 형성을 억제하는 성분인 proline을 함유하고 있으며, 이는 난자의 성숙을 억제하는 성분일 것으로 추정된다.