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Effect of Proline on First Polar Body Formation in Porcine Primary Oocyte  

Oh, H. J. (National Livestock Research Institute)
Lee, E. J. (National Livestock Research Institute)
B. C. Yang (National Livestock Research Institute)
W. K. Chang (National Livestock Research Institute)
Kim, J. S. (Lab. of Reproductive Physiology, Department of Animal Biotechnology, Kyungpook National University)
J. K. Lim (Lab. of Reproductive Physiology, Department of Animal Biotechnology, Kyungpook National University)
Y. K. Yeo (Lab. of Reproductive Physiology, Department of Animal Biotechnology, Kyungpook National University)
M. A. Della-Fera (Department of Foods and Nutrition, Animal and Dairy Science, University of Georgia)
Park, Y. S. (Lab. of Reproductive Physiology, Department of Animal Biotechnology, Kyungpook National University)
Publication Information
Korean Journal of Animal Reproduction / v.26, no.2, 2002 , pp. 165-171 More about this Journal
Abstract
Follicular fluid (FF) contains an oocyte maturation inhibitor with unknown chemical properties. This study was carried out to chemically define the factor(s) inhibiting cumulus cell denudation (CD) and first polar body formation (PBF). Porcine FF (PFF) was extracted with methanol and the extract was serially separated using gel filtration on Superose 12 and Superdex columns. A Superdex fraction was derived with PITC and analyzed with an amino acid analysis column. The results obtained are as follows; PFF had an activity inhibiting both CD and PBF of porcine primary oocytes. Superdex fractions RV2.11 prepared from PFF exhibited an activity inhibiting CD and PBF. By amino acid analysis, the fraction RV2.11 appeared to be proline having the same activity inhibiting CD and PBF. In conclusion, PFF had oocyte maturation inhibitors, of which proline should inhibit CD and PBF.
Keywords
Oocyte maturation inhibitor; Follicular fluid; Proline;
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1 Fetterolf, P. M., Sutherland, C. S., Josephy, P. D., Casper, R. F., and Tyson, J. E. 1994. Preliminary characterization of a factor in human follicular fluid that stimulates human spermatozoa motion. Hum. Reprod., 9:1505-1511   DOI   PUBMED
2 Jagiello, G., Graffo, J., Ducayen, M. and Prosser, R. 1977. Further studies of inhibitors of in vitro mammalian oocyte maturation. Fertil. Steril., 28:476-481
3 Kadam, A. L. and Koide, S. S. 1991. A follicular fluid factor inhibiting Xenopus oocyte maturation. Endocr. Res., 17:343-355   DOI   PUBMED
4 Oh, H. J., Kim, E. H., Shon, C. E., Lee, E. J. and Park, Y. S. 2000. Cumulus oocyte complex expansion inhibiting ingradient in porcine follicular fluid. Kr. J. Emb. Trans., 15:203-210
5 Whitten, W. K. 1971. Adv of Biosci, Raspe G. ed., Vol 6, Pergamon Press. p129
6 Winer-Sorgen, S., Brown, J., Ono, T., Gale, J. A., Campeau, J. D., Marrs, R. P. and Dizerega, G. S. 1986. Oocyte maturation inhibitor activity in human follicular fluid: quantitative determination in unstimulated and clomiphene citrateand human menopausal gonadotropin-stimulated ovarian cycles. J. In Vitro Fert. mbryo Transf., 3(4):218-223   DOI   ScienceOn
7 Channing, C. P., Liu, C. Q., Jones, G. S. and Jones, H. 1983. Decline of follicular oocyte maturation inhibitor coincident with maturation and achievement of fertilizability of oocytes recovered at midcycle of gonadotropin-treatedwomen. Proc. Nat!. Acad. Sci. U. S. A. 80(13): 4184-4188
8 Kadam, A. L. and Koide, S. S. 1990. Identification of hypoxanthine in bovine follicular fluid. J. Pharm. Sci., 79(12):1077-1082   DOI   PUBMED
9 Tarr, G. E. 1986. Methods of protein microcharacterization, JE Shively, ed., Humana Press, Clifton, N. J. pp 155-194
10 Chari, S., Hillensjo, T., Magnusson, C., Sturm, G. and Daume, E. 1983. In vitro inhibition of rat oocyte meiosis by human follicular fluid fractions. Arch. Gynecol., 233:155-164   DOI   ScienceOn
11 Colonna, R. and Mangia, F. 1983. Mechanisms of amino acid uptake in cumulus-enclosed mouse oocytes. Biol. Reprod., 28(4):797-803   DOI   ScienceOn
12 Hillensjo, T., Bronnstrom, M., Chari, S., Daume, E., Magnusson, C., Nilsson, L., Sjogren, A. and Tomell, J. 1985. Oocyte maturation as regulated by follicular factors. Ann. N. Y. Acad. Sci., 442:73-79
13 Vatzias, G. and Hagen, D. R. 1999. Effects of porcine follicular fluid and oviduct-conditioned media on maturation and fertilization of porcine oocytes in vitro. Biol, Reprod., 60(1):42-48   DOI   ScienceOn
14 Romero-Arredondo, A. and Seidel, G. E. Jr. 1994. Effects of bovine follicular fluid on maturation of bovine oocytes. Theriogenology, 41 :383-394   DOI   ScienceOn
15 van de Wiel, D. F., Bar-Ami, S., Tsafriri, A. and de Jong, F. H. 1983. Oocyte maturation inhibitor, inhibin and steroid concentrations in porcine follicular fluid at various stages of the oestrous cycle. J. Reprod. Fertil., 68(1):247-252   DOI   PUBMED   ScienceOn
16 Haghighat, N. and Van Winkle, L. J. 1990. Developmental change in follicular cell-enhanced amino acid uptake into mouse oocytes that depends on intact gap junctions and transport system Gly. J. Exp. Zool., 253(1):71-82   DOI   ScienceOn
17 Hillensjo, T., Batta, S. K., Schwartz-Kripner, A., Webtz, A. C., Sulewski, J. and Channing, C. P. 1978. Inhibitory effect of human follicular fluid upon the maturation of porcine oocytes in culture. J. Clin. Endocrinol. Metab., 47:1332- 1335   DOI   PUBMED
18 Nishizawa, M., Furuno, N., Okazaki, K., Tanaka, H., Ogawa, Y. and Sagata, N. 1993. Degradation of Mos by the N-terminal proline (pro2)-dependent ubiquitin pathway on fertilization of Xenopus eggs: possible significance of natural selection for Pro2 in Mos. EMBO J, 12(10):4021-4027
19 Dostzbrl, J. and Pavlok, A. 1996. Isolation and characterization of maturation inhibiting compound in bovine follicular fluid. Reprod. Nutr. Dev., 36:681-690   PUBMED
20 Downs, S. M. and Eppig, J. J. 1984. Cyclic adenosine monophosphate and ovarian follicular fluid act synergistically to inhibit mouse oocyte maturation. Endocrinology, 114:418-427   DOI   ScienceOn
21 Daen, F. P., Sato, E. and Naito Toyoda Y. 1994. The effect of pig follicular fluid fractions on cumulus expansion and male pronucleus formation in porcine oocytes matured and fertilized in vitro. J. Reprod. Fertil., 101:667-673   DOI   ScienceOn
22 Tsafriri, A. and Channing, C. P. 1975. An inhibitory influence of granulosa cells and follicular fluid upon porcine oocyte meiosis in vitro. Endocrinology, 96:992-997