한국가축번식학회지 (Korean Journal of Animal Reproduction)

한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Effect of Proline on First Polar Body Formation in Porcine Primary Oocyte

  • Oh, H. J. (National Livestock Research Institute) ;
  • Lee, E. J. (National Livestock Research Institute) ;
  • B. C. Yang (National Livestock Research Institute) ;
  • W. K. Chang (National Livestock Research Institute) ;
  • Kim, J. S. (Lab. of Reproductive Physiology, Department of Animal Biotechnology, Kyungpook National University) ;
  • J. K. Lim (Lab. of Reproductive Physiology, Department of Animal Biotechnology, Kyungpook National University) ;
  • Y. K. Yeo (Lab. of Reproductive Physiology, Department of Animal Biotechnology, Kyungpook National University) ;
  • M. A. Della-Fera (Department of Foods and Nutrition, Animal and Dairy Science, University of Georgia) ;
  • Park, Y. S. (Lab. of Reproductive Physiology, Department of Animal Biotechnology, Kyungpook National University)
  • 발행 : 2002.06.01
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초록

난포액에는 난자의 성숙을 억제하는 인자를 함유하고 있으나 화학적 성질이 정확히 알려져 있지않다. 본 연구에서는 1차 난모세포에서 난구세포의 해리와 제1극체의 형성을 억제하는 난포액 성분을 화학적으로 동정하기 위하여 수행되었다 이를 위하여 돼지 난포액을 methanol로 추출한 다음이 추출물을 Superose 12 및 Superdex column을 이용 연속적으로 분리하였으며, Superdex 분절은 PITC로 처리한 다음 아미노산 분석용 column을 이용 분석하였다. 얻어진 결과는 다음과 같다. 난포액은 1차 난모세포에서 난구세포의 해리와 제1극체의 형성을 억제하였다 난포액에서 추출 분리한 Superdex분절 RV2.11 역시 난구세포의 해리와 제1극체의 형성을 억제하였다. 아미노산 분석 결과, 분절 RV2.11은 proline으로 추정되며, proline은 난구세포의 해리와 제1극체의 형성을 억제하였다. 결론적으로 난포액에는 난구세포의 해리와 제1극체의 형성을 억제하는 성분인 proline을 함유하고 있으며, 이는 난자의 성숙을 억제하는 성분일 것으로 추정된다.

Follicular fluid (FF) contains an oocyte maturation inhibitor with unknown chemical properties. This study was carried out to chemically define the factor(s) inhibiting cumulus cell denudation (CD) and first polar body formation (PBF). Porcine FF (PFF) was extracted with methanol and the extract was serially separated using gel filtration on Superose 12 and Superdex columns. A Superdex fraction was derived with PITC and analyzed with an amino acid analysis column. The results obtained are as follows; PFF had an activity inhibiting both CD and PBF of porcine primary oocytes. Superdex fractions RV2.11 prepared from PFF exhibited an activity inhibiting CD and PBF. By amino acid analysis, the fraction RV2.11 appeared to be proline having the same activity inhibiting CD and PBF. In conclusion, PFF had oocyte maturation inhibitors, of which proline should inhibit CD and PBF.

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참고문헌

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