Isolation and identification of secondary metabolites from the roots of Brassica rapa

In order to identify secondary metabolites, the root of Brassica rapa was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$. From the EtOAc and n-BuOH fractions, four secondary metabolites were isolated through the repeated silica gel and octadecyl silica gel (ODS) column chromatographies. From the result of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as 4-(methoxymethyl)phenol (1), ${\alpha}$-methoxy-2,5-furandimethanol (2), phenyl-${\beta}$-D-glucopyranoside (3), and 2-phenylethyl-${\beta}$-D-glucopyranoside (4). They were isolated for the first time from Brassica rapa.

순무(Brassica rapa) 뿌리로부터 이차대사산물의 분리 및 동정

순무 뿌리로부터 활성 물질을 분리 동정하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH 추출물을 얻었다. 이를 EtOAc 분획, n-BuOH 분획, $H_2O$ 분획으로 나누었으며, EtOAc분획과 n-BuOH 분획에 대해 silica gel 및 ODS column chromatography를 실시하여 4종의 이차대사산물을 분리 정제하였다. $^1H-NMR,\;^{13}C-NMR$, DEPT spectrum 및 mass spectrum등을 통하여 4- (methoxymethyl)phonol(1), ${\alpha}$- methoxy-2, 5- furandimethanel (2), phenyl-${\beta}$-glucopyranoside(3) 및 2-phenylethyl-${\beta}$-D -glucopyranoside (4)로 구조를 결정하였다. 이 화합물들은 순무에서는 처음 분리되었다.