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Immunocytochemical Detection of Pneumocystis carinii in Bronchoalveolar Lavage (기관지 폐포 세정액에서 뉴우모시스티스 카리니의 면역세포화학적 검출)

  • Kwon, Kun-Young;Cho, Seung-Che;Kim, Sang-Pyo;Park, Kwan-Kyu;Chang, Eun-Sook;Kim, Chung-Sook
    • The Korean Journal of Cytopathology
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    • v.8 no.1
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    • pp.27-34
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    • 1997
  • Pneumocystis carinli is an established cause of pulmonary infections in immuno-compromised hosts. Several cytoiogical stains, such as Papanicolaou, Gomori methenamine sliver(GMS) and Diff-Quik have been used for detection of the organism, but occasionally can be laborious and, due to a degree of nonspecificity, may be misleading. We evaluated the diagnostic utility of immunocytochenmical stains that recognize P. carinii in bornchoalveolar lavage from experimentally Induced P. carinii pneumonia rats(n=15). In audition to routine stains for diagnosis by morphologic recognition of P. carinii on Papanicolaou, GMS and Diff-Quik stains, bronchoalveolar lavage samples were reacted with immunocytochemical stains using monoclonal antibodies(MAB) 092 and 902. In bronchoalveolar lavage P. carinii organisms were detected In 9 of 10 cases(90%) using each MAB 092 and 902, whereas GMS and Diff-Quik stains demonstrated P. carinii in 13(86%) and 11(73%) of 15 cases respectively. In lung tissue specimens(n=15) P. carinii organisms were well identified on GMS stain and immunohistochemical stains using MAB 092 and 902 in ail cases. We believe that the immunocytochemical staining using MAB 092 and/or 902 is a very useful and diagnostic tool In addition to GMS and Diff-Qulk stain to detect P. carinii organisms in bronchoalveolar lavage.

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Impact of Customer Relationship Management on Customer Loyalty among Patients Visiting a Woman's Hospital (여성전문병원의 고객관계관리(CRM)가 고객충성도에 미치는 영향)

  • Min, Che-Ryu;Kang, Hye-Young;Cho, Woo-Hyun;Lee, Dong-Jin;Kim, Chung-In
    • Korea Journal of Hospital Management
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    • v.13 no.1
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    • pp.65-83
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    • 2008
  • Although a variety of customer relationship management (CRM) activities have been provided by many hospitals in Korea, there lacks empirical evidence on the effect of CRM. The present study was conducted to examine the effect of CRM in terms of the customer response to CRM in a woman's hospital setting. A total of 380 patients receiving inpatient or outpatient care from woman's hospital between October 25 and November 4, 2005 were surveyed for the degree of their experience of and preference for CRM activities of the hospital by 5-point Likert-type scale. Patients were also asked about the level of customer loyalty to the hospital. Eighteen CRM activities offered by the hospital was classified into 4 types of CRM strategies according to Berry and Parasuranman: price, social, structural, and relationship recovery strategy. There's a significant positive correlation between the degree of experience of CRM and preference for CRM(r=0.49, p<0.001). Regression analysis results showed the significant positive relationship between the degree of experience of CRM and customer loyalty(${\beta}$=0.448, p<0.05). Among the 4 CRM strategies, only social(${\beta}$=0.127, p<0.05) and structural strategy(${\beta}$=0.266, p<0.05) showed signifiant positive relationship with customer loyalty. Overall, the favorable customer response to CRM in terms of preference for CRM and customer loyalty indicates that there's a positive effect of CRM on the continuity of the relationship between patients and health care providers.

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Study on the Adaptation of the Official Uniform and the Uniform Color Regulation in the Early Goryeo Dynasty (고려 초 공복제[公服制] 도입과 복색[服色] 운용에 관한 연구)

  • Lim Kyoung-Hwa;Kang Soon-Che
    • Journal of the Korean Society of Costume
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    • v.56 no.1 s.100
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    • pp.131-142
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    • 2006
  • This research has been done based on the Jeon Si Gwa(田柴科) regulation, the monumental inscription material and the former studies. The background of adapting the official uniform regulation in the early Goryeo Dynasty has been surveyed. Differentiating criterion in the official uniform color like purple(紫衫), red(丹衫), red(緋衫), and green(綠衫) has been studied. The official rank had been classified by the color of the official uniform in the early Goryeo uniform regulation. The lower class had worn purple(紫衫). The purple wearing class had not been the government post but the official rank. Those who worked for the Palace had worn purple color since purple color represented the Imperial Palace on which TAI-IL(太一), that is, the highest one of gods dwelt. Those who had worn red(丹色) in the monumental inscription had worked for the job associated with the military service or Hanlim savant(翰林學士) of Kwangmun Academy(光文院) and Hanlim Academy(翰林元). Dan Seo(丹書) which recorded King's commands also means the monumental inscription. In the Goryeosa(高麗史), the military class had been only in Dan Sam(丹衫). That means those class worn Dan Sam(丹衫) could have been as the strong supportive class for the Dynasty. In the early Goryeo Dynasty, the four colored uniform regulation had followed the enforcing policy of the royal authority. It has been assumed that the uniform regulation had been used as a way of enrolling the new supporting class into the existing official system. Dan Sam(丹衫) which had been worn only in the early Goryeo can be seen as the special class for strengthening the royal authority.

Antigenicity of Intralipidos in Guinea Pigs, Mice and Rats (기니픽, 마우스 그리고 랫드에서 Intralipidos의 항원성)

  • Yi, Beoung-Hi;Che, Jeong-Hwan;Li, Guang-Xun;Kang, Byeong-Cheol;Lee, Won-Woo;Ihm, Jong-Hee;Jung, Ji-Youn;Lee, Yong-Soon
    • Toxicological Research
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    • v.14 no.3
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    • pp.459-463
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    • 1998
  • The antigenicity of intralipidos was investigated in guinea pig, mice and rats. Antigenicity tests-active systemic anaphylaxis (ASA), passive systemic anaphylaxis (PSA), passive cutaneous anaphylaxix (PCA) of this materials were performed. The results were followed: 1. After sensitizaion with YPL, YPL+intralipidos, and intralipidos, emulsified with complete Freund's adjuvant (CFA), guinea pigs didn's show any anaphylatic shock symptom in the ASA test, 2. These materials didn't show any anaphylatic shock symptom in the PSA test, 3. After sensitization with antisera of YPL, YPL+intralipidos, and intralipidos sensitized mice, blue spots were not observed on the hypodermis of back of rats in the PCA test. From the results of this investigation, the antigenicity of YPL, intralipidos was negative under the present experimental condition.

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Acute Oral, Intramuscular and Intravenous Toxicity Studies of Recombinant Interferon-$\alpha$2a in Sprague-Dawley Rats

  • Lee, Yong-Soon;Park, Jin-Sung;Che, Jeong-Hwan;Li, Guang-Xun;Kim, Tea-Won;Kim, Hyung-Sub;Park, Jie-Eun;Yun, Jun-Won;Kang, Kyung-Sun
    • Toxicological Research
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    • v.16 no.1
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    • pp.73-76
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    • 2000
  • Acute oral, intramuscluar, and intravenous toxicity studies of recombinant human interferon $\alpha$2$\alpha$(rhIFN $\alpha$2$\alpha$) were performed in Sprague-Dawley (SD) rats. SD rats were administered with doses of 31.25, 62.5, 125, 250 and 500 MIU/kg, respectively, and clinical signs, mortality and body weight changes were observed for 2 weeks. In all animals administered with rhIFN $\alpha$2$\alpha$, there was neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical signs and autopsy findings. Therefore, $LD_{50}$ of rhIFN $\alpha$2$\alpha$ was considered to be higher than 500 MIU/kg in SD rats.

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Phage types of Salmonella enteritidis isolated from animal and human in Korea (한국의 동물과 사람에서 분리한 Salmonella enteritidis의 phage types)

  • Woo, Yong-ku;Park, Mi-sun;Woo, Seung-ryong;Kim, Bong-hwan;Kim, Jae-hak
    • Korean Journal of Veterinary Research
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    • v.40 no.3
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    • pp.515-524
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    • 2000
  • The present study was conducted to investigate the phage types(PT) of Salmonella enteritidis(SE) isolated from animals and human patients in Korea during the period 1993-1999. SE were isolated from chickens including the layer and broiler, duck, rat, swine and human patients in nationwide scale. A total of 231 non-human and 14 human SE strains were phage typed and 80.4%(197/245) of SE isolates tested could discriminate as a ten SEPTs including the RDNC by the 10 standard phages, which were brought from the Central Public Health Laboratory in England. In analysing of phage types both animal and human SE isolates, SEPT 4(55.4%) was the most prevalent in domestic animals including the layer(39.3%) and broiler(64.5%), duck(55.5%), and swine(75.0%) since 1993. About 57% of the S enteritidis from human was SEPT 4. Along with SEPT 4, SEPT 1, SEPT 6a, SEPT 7 and SEPT 7a var, SEPT 9b, SEPT 15, SEPT 21b and SEPT 22 were also found from animal and human SE isolates. SEPT 4(40.0%) was isolated from frozen chicken meats imported from China along with SEPT 7 and 7a var strains in this study. In conclusion, SEPT 4 was the most predominant SEPT in our country regardless of animal or human hosts including the Che-Ju island and almost exclusively found in domestic poultry(layer and broiler). During the review period, SEPT 4 has already been spreading continually among the animal hosts since 1993.

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An Analysis of Color Status and Image Evaluation of the Outdoor Advertisement for Improving the Outdoor Facade of Commercial Building Structure in Old Downtown Area (구도심가로변의 상업건축물 외부파사드 개선을 위한 옥외광고물의 색채현황 분석 및 이미지 평가)

  • Choi, Young-Sin;Lim, Che-Zinn;Lee, Jin-Sook
    • Korean Institute of Interior Design Journal
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    • v.20 no.1
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    • pp.208-219
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    • 2011
  • This study is intended to formulate the issues through the status analysis and image evaluation for each street on the basis of colors for the outdoor advertisements for part of street side in the oldest downtown for its construction year. Analysing the business type of the street side, it displays the identity of the fashion business area and the color status shown on the outdoor advertisement did not consider the harmony on the other business type and building improvements that it displays chaotic street views in overall. Status of color for the outdoor advertisement compared and analyzed for each street-side to be analyzed with high color contrast with the building structure and outdoor advertisements than the Street B side where there are many businesses of fashion sundries and fashion clothes to form more complicated street scenery visually for the Street A side. The color combination principle of building structure and outdoor advertisement was shown to be the factor for the contrasting unity and diversity. In order not to stimulate this visual confusion, the colors of outdoor advertisement has to be applied on the basis of the color guideline based on the color combination principle of outdoor advertisement and building structure to have the aesthetic harmony overall. As a result of analysis using the KJ method, the present image of the old downtown area was shown with the adjective vocabularies of "complicated", "out-dated", "chaotic", "disorganized", "dirty", "suffocating", and "unilateral", and its image to strive for would be in a total of 6 adjective vocabularies of "well-arranged", "young", "dynamic", "sophisticated", "personable" and "neat", and it has presented the basic foundation of color guideline of outdoor advertisement fit for its image.

A Study on a concept of Food Accumulation using Multidimensional Scaling - Comparison between Pyungweesan Questionnaire and Health Related Questionnaires - (다차원척도법을 이용한 식적(食積)의 개념에 관한 고찰 - 평위산(平胃散) 변증(辨證) 설문지와 건강 관련 설문지와의 비교 연구 -)

  • Lee, Cheong-Hee;Park, Young-Jae;Kim, Min-Yong;Lee, Sang-Chul;Park, Young-Bae
    • The Journal of the Society of Korean Medicine Diagnostics
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    • v.15 no.2
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    • pp.141-148
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    • 2011
  • Objectives: In comparison with functional dyspepsia, food accumulation is known to have wider concept. On this study, we will make a proposal on the concept of food accumulation by a statistical comparison between pyungweesan questionnaire and health related questionnaires. Methods: We studied the similarities between six factors of pyungweesan questionnaire and four health related questionnaires(Rome II criteria of functional dyspepsia, subjective symptoms of fatigue test, beck depression inventory, state-trait anxiety inventory, etc.) using multidimensional scaling. Results and Conclusions: 1. Physical-emotional axis and acute-chronic axis were labelled in a two-dimensional scaling solution. 2. Seup-dam(濕痰) factor and sik-che(食滯) factor of food accumulation were closely related to fatigue. 3. Bi-seup(脾濕) factor of food accumulation was closely related to depression and anxiety. 4. The findings suggest that a concept of food accumulation reflects some aspects of physical fatigue and emotional depression as well as dyspepsia.

A case report of Cheyletiella infestation on a Whippet dog in Korea (Whippet종 개에서의 Cheyletiella yasguri(진드기목: 발톱진드기과) 감염증례 보고)

  • 신성식
    • Parasites, Hosts and Diseases
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    • v.34 no.4
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    • pp.267-278
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    • 1996
  • A clinical case of CheMletietlo infestation on a dog born and raised in Korea is reported. A three-year old female Whippet was hospitalized due to a multiple fracture and displacement of the left scapula caused by a recent car accident The mite infestation was not noticed at the time of hospitalization. The dog underwent multiple operations involving internal fixation of the fractured scapula with wire and a plate, followed by extensive chemotherapy with antibiotics and prednisolone. After two months of hospitalization. a pruritic dermatitis near the left scapula developed. Multiple white dandruff-like ilakes were seen on the hair coat. especially over the dorsal spine and neck, and the dog expressed increased pruritus by frequently licking and scratching the affected areas. Local dense accumulations of skin debris that became crusty were also observed. Microscopic examination or a skin scraping revealed a heavy infestation of cheyletiella vnsfuri. as idrntified by the prrsrnce of holes of the palpi and the heart-shaped sensory organ on menu I. Immnosuppression elicited by the extensive administration of prednisolone was suspected for the initiation of the generalized mite infestation.

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The Effect of Additives in Final Rinse Water on Soiling and the Removal of Soil. (세탁후 최종처리액의 조성이 직물의 오염 및 세척성에 미치는 영향)

  • Cho Sung Kyo;Kim Sang Reon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.2 no.1
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    • pp.167-176
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    • 1978
  • The effect of additives in final rinse water during laundering on soiling, soil removal and some properties of fabrics has been studied with various fabrics. The additives examined were fabric softener (Sta-Puf), cationic surfactant (Apole PS), sizing materials such as CMC, PYA, cornstarch and mixture of CMC and cationic surfactant. The results obtained may be summerized as follows. L Addition of additives except PVA in final rinse water generally reduce the deposition of carbon-$CCl_4$ soil and it seems to be rather independant of the concentration of additives. The effect of additives on soil resistant is found to increase in the following order. cotton; Apole

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