• Asian Pacific Journal of Cancer Prevention
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• 제16권8호
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• pp.3505-3508
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• 2015

Background: Ras genes are thought to play an important role in human cancer since they have been found to be activated frequently in several types of tumors including breast cancer, where the overall incidence of K-RAS oncogene activation is 0-10%. Evaluation of K-RAS gene not only for mutational frequency but also for mutation types in this downstream signaling gene pathway is necessary to determine the mechanisms of action. The present study was conducted to test the hypothesis that K-RAS activation is involved in breast cancer risk of south Indian population. Materials and Methods: A total of 70 paired pathologically confirmed tumor and non-tumor tissues from the same breast cancer patients were analysed for most common K-RAS mutations of codon 12,13 and 61 by polymerase chain reaction followed by restriction digestion and direct nucleotide sequencing method. Results: We found that a high rate of homozygous and heterozygous mutations of codon 12, but not codon 13 and 61, may influence the invasive ductal carcinoma of breast risk in this study. Conclusions: Our study indicated that only codon 12 may be involved in initiating breast carcinogenesis in India.

• 한국환경보건학회지
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• 제32권2호
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• pp.126-131
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• 2006

The objective of this study was monitoring of genetically modified soybean by PCR and ELISA. We collected 60 soybean samples from the open markets located in Western Gyeongnam (Sacheon, Hamyang, Hadong, Sanchung, Uiryung, Geochang, and Hapcheon). A total of 60 soybeans was examined and 14 genetical modified soybean (GMS) were detected by PCR. The GMS rate of selling soybean in Uiryung, Hadong, Sacheon, and Hapcheon was 50.0%, 37.5%, 33.3% and 25%, respectively. The 7 of 14 GMSs were positive by ELISA and most of positive samples were below 3% GMS but 1 (Uiryung 1) of the positive samples was over the 3% which is maximum permit limit in Korea. These results mean that merchants of open market did not express for selling soybean mixed with GMS, so consumers did not recognize GMO. Therefore, we thought that education of GMO for merchant of open market need to recognize about GMO maximum permit limit.

• 농촌지도와개발
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• 제3권2호
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• pp.247-264
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• 1996

The objective of this study was to analyze the reality of the program development process in detail accomplished by the organizations of extension education in through the hermeneutical approach. To achieve this purpose, this study tried to find answers to the following three questions : (1) how the organization of extension education develop their programs for farmers? (2) what is the programmers' awareness to the process of program development? and what is the reason for their thought and action to do so. (3) can be discussed integrally the findings for drawing some implications? The qualitative data were mainly gathered through participation observation and unstructured interview. And the qualitative data were analyzed by (1) noting pattern and themes, (2) seeing plausibility, (3) clustering, (4) making metaphors, (5) factoring, and (6) building a logical chain of evidence which hermeneutical techniques far drawing the meaning from the gathered data. The findings of this study were as follows: The programmers of this organization carried out the developing program in the technical training division at the national level and the section of fostering farm manager at the county level for the purpose of delivering the agricultural new technology and the agricultural policy. The term used formly in this organization was `curriculum development for former', and the reality of program was `instruction profile` which signified the set of the educational manin contents. At county level, Educational planning was stressed on the implement of educational administration.

• Journal of Photoscience
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• 제7권4호
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• pp.143-148
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• 2000

Studies have been conducted to explore single electron transfer(SET) promoted photocyclization of ($\omega$-phthalimidoalkoxy)acetic acids(alkoxy=ethoxy, n-propoxy and n-butyloxy). Photocyclizations occur in methanol or acetone in high yields to produce cyclized products in which phthalimide carbonyl carbon is bonded to the carbon of side chain in place of the carboxylic group. These photocyclizations are thought to proceed through pathways involving intramolecular SET from oxygen in the $\alpha$-carboxymethoxy groups to the singlet excited state phthalimide moieties followed by decarboxylation of the intermediate $\alpha$-carboxymethoxy cation fadicals and cyclizations by radical coupling. The photocyclizations occur ca. three times faster in both methanol or acetone with one equivalent of sodium hydroxide added to the reactions and occur slower in acetone than in methanol. The efficient and regiselective cyclization reactions observed for photolyses in methanol represent synthetically useful processes for construction of heterocyclic compounds.

• 정보교육학회논문지
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• 제16권3호
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• pp.363-371
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• 2012

본 연구는 프로그래밍 교육에 구성주의 교수-학습을 적용하여 프로그래밍 습득 및 전이에 미치는 영향을 분석해 보는데 목적이 있다. 이에 따라 프로그래밍 학습을 위한 구성주의 기반 교수 환경을 설계하고 이러한 환경에서 실제로 수업을 실시하였다. 본 연구의 목적 및 선행 연구에 기초하여 고안된 연구 도구를 사용하여 프로그래밍 학습 및 태도에 미치는 효과 및 관련 변인들을 측정 분석하였다. 분석 결과 구성주의 기반 프로그래밍 교수-학습 과정에서 연구 대상의 학년 및 교수 환경에 따라 유의미한 효과가 있었으며 긍정적인 영향을 미치는 것으로 나타났다. 본 연구의 결과에 대한 시사점 및 후속 연구에 대한 제언을 제시하였다.

• 무역학회지
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• 제47권5호
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• pp.241-251
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• 2022

As a 4th industrial revolution technology, robots are changing the form of labor market and trade in Korea. In the future, changes in the international trade order are expected to move in the direction of shortening global supply chains and restricting trade between countries. Accordingly, reshoring of relocating overseas production facilities to Korea or near-shoring of relocating overseas production facilities to neighboring allies may expand. In this context, this study analyzed the impact of robot introduction on the domestic labor market and trade based on firm-level data. As a result of analysis based on the 'business activity data' accumulated from 2017 to 2019, the introduction of robot technology was analyzed to expand low-wage, low-skilled employment. Analysis on trade shows that the introduction of robots decreases exports and increases imports. In order to expand exports through the technology of the 4th industrial revolution, employment expansion and robot introduction should occur at the same time, rather than replacing the labor force with robots. In addition, it is thought that reshoring's goal of risk management can be achieved when a stable supply chain for imports of raw materials or essential goods, which are difficult to transfer to Korea, is established together.

• BMB Reports
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• 제41권12호
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• pp.863-867
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• 2008

CD320 has been recently discovered and reported as a follicular dendritic cell (FDC) protein. Although CD320 is known to enhance proliferation of germinal center (GC) B cells, little other information is available. In this study, we investigated its cellular distribution in the GC. Confocal microscopy of human tonsil sections revealed co-localization of CD320 with CD19 and CD38 but not with CD3 indicating that GC B cells expressed CD320 in addition to FDC. In purified GC B cells, CD320 expression was inhibited in the nucleus, membrane and cytoplasm. Reverse transcriptase-polymerase chain reaction confirmed CD320 mRNA expression in B cells. These finding indicate that CD320 is expressed in B cells in addition to FDC, and that its GC activity may be more complicated than previously thought.

• 대한화학회지
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• 제31권6호
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• pp.582-589
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• 1987

SIAS unit로 개질된 PET의 열적 성질을 조사한 결과, SIAS unit의 함량이 증가함에 따라 $T_g$는 증가, $T_m$은 감소하는 경향을 보였으며 결정화속도는 크게 감소함을 관찰할 수 있었다. 결정화속도의 감소는 극성과 크기가 큰 술폰산 나트륨기가 쇄의 유연성을 크게 감소시켜 결정표면으로 확산 이동한 고분자 쇄가 결정형태로 재배치 하는 것을 방해하는 것이 중요한 원인으로 생각되며, 결정성장기구는 SIAS unit의 함량에 의해 크게 좌우되어 SIAS unit의 함량이 증가할 수록 3차원 공간으로의 결정 성장이 제한 받음을 관찰 할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제26권6호
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• pp.1087-1097
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• 2016

An intracellular lipase from Anoxybacillus flavithermus HBB 134 was purified to 7.4-fold. The molecular mass of the enzyme was found to be about 64 kDa. The maximum activity of the enzyme was at pH 9.0 and 50℃. The enzyme was stable between pH 6.0 and 11.0 at 25℃, 40℃, and 50℃ for 24 h. The K_m and V_max of the enzyme for pNPL substrate were determined as 0.084 mM and 500 U/mg, respectively. Glycerol, sorbitol, and mannitol enhanced the enzyme thermostability. The enzyme was found to be highly stable against acetone, ethyl acetate, and diethyl ether. The presence of PMSF, NBS, DTT and β-mercaptoethanol inhibited the enzyme activity. Hg²⁺, Fe³⁺, Pb²⁺, Al³⁺, and Zn²⁺ strongly inhibited the enzyme whereas Li⁺, Na⁺, K⁺, and NH₄⁺ slightly activated it. At least 60% of the enzyme activity and stability were retained against sodium deoxycholate, sodium taurocholate, n-octyl-β-D-glucopyranoside, and CHAPS. The presence of 1% Triton X-100 caused about 34% increase in the enzyme activity. The enzyme is thought to be a true lipase since it has preferred the long-chain triacylglycerols. The lipase of HBB 134 cleaved triolein at the 1- or 3-position.

• Journal of Applied Biological Chemistry
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• 제64권1호
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• pp.49-55
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• 2021

The diet plays a fundamental role in the formation of the gut microbiota, determining the interrelationship between the gut microbiota and the host. The current study investigated the effect of Chlorella vulgaris on the gut microbiota by using simulated in vitro digestion and colonic fermentation. Bioaccessibility was measured after in vitro digestion, and SCFAs and microbial profiling were analyzed after colonic fermentation. The bioaccessibility of C. vulgaris was 0.24 g/g. The three major SCFAs (acetate, propionate, and butyrate) increased significantly when compared to the control group. In microbial profiling analysis, microorganisms such as Faecalibacterium, Dialister, Megasphaera, Dorea, Odoribacter, Roseburia, Bifidobacterium, Butyricmonas, and Veillonella were high in C. vulgaris group. Among them, Faecalibacterium, Dialister, Megasphaera, Roseburia, and Veillonella were thought to be closely associated with the increased level of SCFAs. Finally, it can be expected to help improve gut microbiota and health through ingestion of C. vulgaris. However, further studies are vital to confirm the changes in the gut microbiota in in vivo, when C. vulgaris is ingested.