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Expression of CD320 in human B cells in addition to follicular dendritic cells

  • Cho, Wha-Jung (Department of Microbiology and Immunology, Kangwon National University College of Medicine) ;
  • Choi, Jin-Suk (Department of Microbiology and Immunology, Kangwon National University College of Medicine) ;
  • Park, Chan-Hum (Department of Otorhinolaryngology, Hallym University College of Medicine) ;
  • Yoon, Sun-Ok (Laboratory of Cellular Immunology, Ochsner Clinic Foundation) ;
  • Jeoung, Doo-Il (Vascular System Research Center, Kangwon National University) ;
  • Kim, Young-Myeong (Vascular System Research Center, Kangwon National University) ;
  • Choe, Jong-Seon (Department of Microbiology and Immunology, Kangwon National University College of Medicine)
  • Published : 2008.12.31

Abstract

CD320 has been recently discovered and reported as a follicular dendritic cell (FDC) protein. Although CD320 is known to enhance proliferation of germinal center (GC) B cells, little other information is available. In this study, we investigated its cellular distribution in the GC. Confocal microscopy of human tonsil sections revealed co-localization of CD320 with CD19 and CD38 but not with CD3 indicating that GC B cells expressed CD320 in addition to FDC. In purified GC B cells, CD320 expression was inhibited in the nucleus, membrane and cytoplasm. Reverse transcriptase-polymerase chain reaction confirmed CD320 mRNA expression in B cells. These finding indicate that CD320 is expressed in B cells in addition to FDC, and that its GC activity may be more complicated than previously thought.

Keywords

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