Browse > Article

Monitoring of Genetical Modified Soybean Sold at Local Open Market in Western Gyeongnam  

Shim Won-Bo (Division of Applied Life Science, Graduate School of Gyeongsang National University)
Nam Baek-Sang (Division of Applied Life Science, Graduate School of Gyeongsang National University)
Choe Ju-Mi (Division of Applied Life Science, Graduate School of Gyeongsang National University)
Jeong Soon-Chun (LMO Evaluation Laboratory, Korea Research Institute of Bioscience and Biotechnology)
Chung Duck-Hwa (Division of Applied Life Science, Graduate School of Gyeongsang National University)
Publication Information
Journal of Environmental Health Sciences / v.32, no.2, 2006 , pp. 126-131 More about this Journal
Abstract
The objective of this study was monitoring of genetically modified soybean by PCR and ELISA. We collected 60 soybean samples from the open markets located in Western Gyeongnam (Sacheon, Hamyang, Hadong, Sanchung, Uiryung, Geochang, and Hapcheon). A total of 60 soybeans was examined and 14 genetical modified soybean (GMS) were detected by PCR. The GMS rate of selling soybean in Uiryung, Hadong, Sacheon, and Hapcheon was 50.0%, 37.5%, 33.3% and 25%, respectively. The 7 of 14 GMSs were positive by ELISA and most of positive samples were below 3% GMS but 1 (Uiryung 1) of the positive samples was over the 3% which is maximum permit limit in Korea. These results mean that merchants of open market did not express for selling soybean mixed with GMS, so consumers did not recognize GMO. Therefore, we thought that education of GMO for merchant of open market need to recognize about GMO maximum permit limit.
Keywords
polymerase chain reaction; genetical modified organism; soybean; monitoring;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Straub, J. O. : Environmental risk assessment for new human pharmaceutivals in the Europian Union according to the draft quideline/discussion paper of January 2001. Toxicology Letters, 135(3), 231-237, 2002   DOI   ScienceOn
2 정명은, 정석찬, 김계희, 김성일, 송시욱, 조남인 : 식육가공품에서 GMO(Genetically Modified Organism)검출을 위한 PCR방법 개발 및 적용에 관한 연구 I. PCR법을 이용한 GMO 검사방법 개발. 한국수의공중보건학회지, 28(2), 97-107, 2004
3 식품의약품안전청 : 유전자재조합식품 등의 표시기준. 식품의약품안전청고시 제2001-43호, 2001
4 김동술, 정덕화, 이용욱 : 곡류 중 Ochratoxin A의 검색을 위한 면역분석법에 관한 연구. 한국환경보건학회지, 20(3), 54-60, 1994
5 김동술, 송재영, 정덕화 : ELISA법에 의한 마우스의 혈청 및 조직중의 T-2 toxin의 검색. 한국환경보건학회지, 22(1), 51-56, 1996
6 신원선, 김명희 : 유전자재조합 감자의 검정을 위한 DNA 분리 및 PCR 검출의 최적조건 검색. 한구식품과학회지, 35(4), 591-597, 2003
7 Hubner, P., Studer, E. and Lithy, J. : Quantitative competitive PCR for the detection of genetically modified organisms in Food. Food Control, 10, 353-358, 1999   DOI   ScienceOn
8 안종주, 백남원 : 여론 주도 집단의 환경보건 위해물질에 대한 인식도와 그 관리 정책에 관한 연구 -유전자재조합식품과 내분비계 장애물질을 중심으로. 한국환경보건학회지, 31(5), 431-443, 2005   과학기술학회마을
9 ILSI report : Development in relation to regulatory requirements for the detection of GMOs in food chain. 2000
10 박선희, 곽효선, 박용춘, 문희숙, 우건조, 김창민 : 유전재재조합식품 검사법 확립. 식품의약품안전청 연구보고서, 2, 32-46, 2001
11 김영찬, 이철수, 황옥순, 김성조, 이영옥, 윤성원, 서정화, 남용석 : 대두 및 옥수수 가공식품에서 유전자재 조합체(GMO)의 정성 PCR분석을 위한 핵산 추출방법 별 비교. 한국식품위생안전성학회지, 18(1), 6-13, 2003
12 Achet, E., Martin, G. G., Vigneau, F. and Meyer, G. : Detection of genetically modified organisms(GMOs) by PCR : a brief review of methodologies available. Food Sci. & Tech., 9, 380-388, 1999
13 Tengel, C., $Schu{\beta}er$, P., Setzke, E., Balles, J. and $Sprenger-Hau{\beta}les$ M : PCR-based detection of genetically modified soybean and maize in raw and highly processed foodstuffs. Biotechniques, 31, 426-429, 2001
14 Marsuoka, T., Kuribara, H., Akiyama, H., Miura, H., Goda, Y., Kusakabe, Y., Isshiki, K., Toyoda, M. and Hino, A. : A multiplex PCR method of detecting recombinent DNAs from five lines of genetically modified maize. J. Food Hyg. Soc. Japan, 42(1), 24-32, 2000
15 식품의약품안전청 : 유전자재조합식품 . 식품첨가물 안전성 평가자료 심사지침 개정고시. 식품의약품안전청고시 제2003-37호, 2003
16 허유행 : 대두발효를 위한 Bacillus subtilis 균주의 실제분리에 관한 연구. 한국환경보건학회지, 12(2), 76-74, 1986
17 농림부 : 유전자변형 농산물 표시요령. 농림부고시 제2000-31호, 2000
18 Padgette, S. R., Kolacz, K. H., Delannay, X., Re, D. B., Lavallee, J. B., Tinius, C. N., Rhodes, W. K., Otero, I. Y., Barry, G. F., Eichholtz, D. A., Peschke, V. M., Nida, D. L., Taylor, N. B. and Kishore, G. M. : Development, identification and characterization of a glyphosate-tolerant soybean line. Crop Sci., 35, 708-715, 1995   DOI   ScienceOn
19 식품의약품안전청 : 유전자재조합식품 검사지침, 2002
20 ILSI report : Methods for novel foods derived from genetically modified organisms. 1998
21 Brett, G. M., Chambers, S. J., Huang, L. and Morgan, M. R. A. : Design and development of immunoassays for detection of proteins. Food Control, 10, 401-406, 1999   DOI   ScienceOn
22 임송수, 박용하 : 유전자변형 농산물의 관리 및 표시에 관한 정책 연구. 한국농촌경제연구원 보고서, R433, 2001