• Title/Summary/Keyword: cell stability

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CAPACITY ANALYSIS OF THE SILVER OXIDE-ZINC CELL (PHASE 2)

  • 이완구
    • Journal of the Korean Professional Engineers Association
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    • v.15 no.1
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    • pp.36-40
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    • 1982
  • This study is to characterize Ago stability useful for manufacturing silver oxide button cell, and deter mined by test tube gassing evaluations and by cell expansion and further discuss en the effectiveness of pellet reduction treatments to obtain single plateau discharge Profile and relevent performance.

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Dimensional Change of Acetylated Hardwood (아세틸화 활엽수재의 치수변화 특성)

  • 한규성
    • Journal of the Korea Furniture Society
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    • v.11 no.2
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    • pp.79-84
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    • 2000
  • It is well-known fact that dimensional stability of wood is greatly enhanced by acetylation of wood. This dimensional stability results from bulking of the reacted acetate within the cell wall, which reduces further swelling when the modified woods come into contact with water or water vapor. The purpose of this research was to determine the water absorption and dimensional stability of the acetylated solid wood in liquid water and in humidity tests. Beech and red oak were acetylated. Moisture and water absorption of acetylated wood were quite dependent on weight percent gain(WPG). Antiswelling efficiency(ASE) was quite dependent on WPG, but was not dependent on species.

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Analysis of Slope Stability using Cell Unit Evaluation (셀 단위 평가법을 이용한 사면의 안정성 평가)

  • Jang, Bo-An;Poong, Bo-Hyun;Jang, Hyun-Shic
    • The Journal of Engineering Geology
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    • v.18 no.4
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    • pp.405-414
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    • 2008
  • When we evaluate slope stability, we regard the slope homogeneous and evaluate slope stability at the most dangerous portion of slope. However, since conditions and properties of rock mass/soil are different from one location to another within a single slope, slope stability evaluated by current concept can not represent slope correctly. This also result in over-reinforcement at the portion where reinforcement is not necessary. In order to solve these problems, we suggest a cell unit evaluation method in which we apply small rectangular cells in a slope and regard each cell as a single slope. In this method, slopes are classified into soil slope and rock slope depending on materials. Strength of rock, volumetric joint count, spacing of joints, condition of joints, ground water condition and so on are examined and SMR and condition index values are calculated. Finally, all data and results are presented as contour maps. We apply the cell unit evaluation method into 3 cut slopes. SMR values estimated by the new method are larger than those by current concept at most portions of slope, indicating that the new method suggested by this research represent slope stability more correctly than methods which were used. This method will prevent over-reinforcement at the portion of slope where reinforcement is not necessary.

Effect of Ginseng Polysaccharide on the Stability of Lactic Acid Bacteria during Freeze-drying Process and Storage

  • Yang, Seung-Hyun;Seo, Sung-Hoon;Kim, Sang-Wook;Choi, Seung-Ki;Kim, Dong-Hyun
    • Archives of Pharmacal Research
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    • v.29 no.9
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    • pp.735-740
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    • 2006
  • Lactic acid bacteria (LAB) quickly attenuate or are killed during the freeze-drying process and storage. The effect of some natural polysaccharides, which are known as potent antitumor and immunomodulating substances, on the viability of the LAB, Lactobacillus acidophilus and Bifidobacterium breve, on freeze-drying and storage were investigated. Among the polysaccharides tested, red ginseng polysaccharide (RGP) and chitosan significantly inhibited the cell death of the LAB during freeze-drying, and fucoidan and RGP most potently protected the cell death of the LAB during storage. The stabilities of the LAB on the addition of RGP and fucoidan were comparable to that of skimmed milk. However, white ginseng polysaccharide (WGP) did not promote storage stability. When 5% skimmed milk/5% RGP treated LAB were freeze-dried and stored, their viabilities were found to be significantly higher those treated with 5% or 10% RGP. The stabilizing effect of 5% RGP/5% skimmed milk during LAB freeze-drying and storage stability was comparable to that of treatment with 10% skimmed milk. Based on these findings, we believe that RGP beneficially improves the stability of LAB during the freeze-dry process and storage.

Stability of Li[Co0.1Ni0.15Li0.2Mn0.55]O2 Cathode Material for Lithium Secondary Battery (리튬 2차 전지용 Li[Co0.1Ni0.15Li0.2Mn0.55]O2 양극물질의 안정성 고찰)

  • Park, Yong-Joon
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.20 no.5
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    • pp.443-449
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    • 2007
  • The structural and thermal stability of $Li[Co_{0.1}Ni_{0.15}Li_{0.2}Mn_{0.55}]O_2$ electrode during cycling process was studied. The sample was prepared by simple combustion method. Although there were irreversible changes on the initial cycle, O3 stacking for $Li[Co_{0.1}Ni_{0.15}Li_{0.2}Mn_{0.55}]O_2$ structure was retained during the first and subsequent cycling process. Impedance of the test cell was decreased after the first charge-discharge process, which would be of benefit to intercalation and deintercalation of lithium ion on subsequent cycling. As expected, cycling test for 75 times increased impedance of the cell a little, instead, thermal stability of $Li[Co_{0.1}Ni_{0.15}Li_{0.2}Mn_{0.55}]O_2$ was improved. Moreover, based on DSC analysis, the initial exothermic peak was shifted to high temperature range and the amount of heat was also decreased after cycling test, which displayed that thermal stability was not deteriorated during cycling.

Preparation and Characterization of SPAES/SPVdF-co-HFP Blending Membranes for Polymer Electrolyte Membrane Fuel Cells (고분자 전해질 연료전지용 술폰화된 폴리(아릴렌 이써 설폰)/SPVdF-co-HFP 브렌딩 멤브레인의 제조 및 특성 분석)

  • PARK, CHUL JIN;KIM, AE RHAN;YOO, DONG JIN
    • Transactions of the Korean hydrogen and new energy society
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    • v.30 no.3
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    • pp.227-236
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    • 2019
  • In this work, preparation and characterizations of hybrid membranes containing sulfonated poly(arylene ether sulfone) (SPES) and sulfonated poly(vinylidene fluoride-co-hexafluoropropylene) (SPVdF-co-HFP) (20, 30 or 40 wt%) were carried out. The structure of hybrid membranes was confirmed using X-ray diffraction (XRD) analysis and the Fourier transform infrared (FT-IR) spectroscopy. The prepared SPAES/SPVdF-30 membrane exhibits higher ionic conductivity of 68.9 mS/cm at $90^{\circ}C$ and 100% RH. Besides, the other studies showed that the hybrid membrane has good oxidation stability, thermal stability, and mechanical stability. Thus, we believe that the prepared hybrid membrane is suitable for the development of membranes for fuel cell applications.

PEG Distribution in the Cell Walls of PEG-treated Woods using SEM-EDS (SEM-EDS를 이용한 PEG처리재 세포벽내의 PEG분포 분석)

  • Kwon, Gu-Joong;Kim, Nam-Hun
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.4
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    • pp.9-14
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    • 2006
  • This study was carried out to investigate the distribution and penetration of PEG molecules into the cell wall of wood by SEM-EDS method. SEM observation indicated that the cell walls of PEG-treated specimen were swollen, and didn't show any change in their shapes, while the cells of untreated specimen were more or less collapsed. The results of X-ray analyses of potassium stained samples revealed the distribution of the impregnated chemicals within the cell walls, when considering the distribution of potassium ions in the cell walls. Consequently, this study supported the possibility of PEG molecules penetrated into the wood cell wall play an important role in enhancing the dimensional stability of wood.

Assessment of Immune Parameters of the Wild Pacific Oyster (Crassostrea gigas) using a Flow Cytometry and Neutral Red Retention Assay (유세포 분석기와 Neutral Red Retention Assay를 이용한 자연산 참굴(Crassostrea gigas)의 면역력 측정)

  • Hong, Hyun-Ki;Kang, Hyun-Sil;Kim, Young-Ok;Choi, Kwang-Sik
    • Ocean and Polar Research
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    • v.34 no.2
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    • pp.137-149
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    • 2012
  • Hemocyte parameters of the wild Pacific oyster Crassostrea gigas inhabiting intertidal zones in small bays (Gwangyang and Jinhae Bay) on the southern coast of Korea were evaluated using flow cytometry and neutral red retention (NRR) assay. Morphological features, cell count, mortality, DNA damage, phagocytosis, and lysosomal membrane stability of hemocytes were analyzed. Three types of hemocytes were identified in the oyster hemolymph: granulocytes, hyalinocytes, and blast-like cells. Immune related functions of hemocyte including phagocytosis and lysosomal membrane stability were significantly different among the study areas (P<0.05), while cell count, mortality, and DNA damage of hemocytes were not significantly different. In Gwangyang Bay, phagocytosis of granulocytes and lysosomal membrane stability of oyster hemocytes inhabiting inside bay were significantly lower than those of oyster hemocytes in outside bay (P<0.05), indicating that oysters in inside bay of Gwangyang were relatively suppressed the immunological function in hemocytes. Contrary to Gwangyang Bay, immune parameters of oyster hemocytes in Jinhae Bay not showed the difference between sampling sites. In conclusion, flow cytometry and NRR assay using oyster hemocyte has a powerful tool to investigate the cell level in a short time due to no-preprocessing of material.

PKCθ-Mediated PDK1 Phosphorylation Enhances T Cell Activation by Increasing PDK1 Stability

  • Kang, Jung-Ah;Choi, Hyunwoo;Yang, Taewoo;Cho, Steve K.;Park, Zee-Yong;Park, Sung-Gyoo
    • Molecules and Cells
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    • v.40 no.1
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    • pp.37-44
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    • 2017
  • PDK1 is essential for T cell receptor (TCR)-mediated activation of $NF-{\kappa}B$, and PDK1-induced phosphorylation of $PKC{\theta}$ is important for TCR-induced $NF-{\kappa}B$ activation. However, inverse regulation of PDK1 by $PKC{\theta}$ during T cell activation has not been investigated. In this study, we found that $PKC{\theta}$ is involved in human PDK1 phosphorylation and that its kinase activity is crucial for human PDK1 phosphorylation. Mass spectrometry analysis of wild-type $PKC{\theta}$ or of kinase-inactive form of $PKC{\theta}$ revealed that $PKC{\theta}$ induced phosphorylation of human PDK1 at Ser-64. This $PKC{\theta}$-induced PDK1 phosphorylation positively regulated T cell activation and TCR-induced $NF-{\kappa}B$ activation. Moreover, phosphorylation of human PDK1 at Ser-64 increased the stability of human PDK1 protein. These results suggest that Ser-64 is an important phosphorylation site that is part of a positive feedback loop for human PDK1-$PKC{\theta}$-mediated T cell activation.

Deubiquitinase USP35 as a novel mitotic regulator via maintenance of Aurora B stability

  • Park, Jinyoung;Song, Eun Joo
    • BMB Reports
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    • v.51 no.6
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    • pp.261-262
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    • 2018
  • Aurora B is an important kinase involved in dynamic cellular events in mitosis. Aurora B activity is controlled by several post-translational modifications (PTMs). Among them, E3 ubiquitin ligase-mediated ubiquitination plays crucial roles in controlling the relocation and degradation of Aurora B. Aurora B, ubiquitinated by different E3 ligases, moves to the exact site for its mitotic function during metaphase-anaphase transition and is then degraded for cell cycle progression at the end of mitosis. However, how the stability of Aurora B is maintained until its degradation has been poorly understood. Recently, we have found that USP35 acts as a deubiquitinating enzyme (DUB) for Aurora B and affects its stability during cell division, thus being involved in the regulation of mitosis. In this review, we discuss the USP35-mediated deubiquitination of Aurora B and the regulation of mitotic progression by USP35.