• BMB Reports
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• v.34 no.4
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• pp.342-346
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• 2001

The Mycobacterium bovis BCG panB gene, encoding ketopantoate hydroxymethyltransferase (KPHMT), was cloned from a ${\lambda}gt11$ genomic library and sequenced. The DNA sequence encodes a protein that contains 281 amino acid residues (M, 29,337) with a high similarity to the KPHMTs. Subcloning of a 846 by open reading frame (ORF), but not a 735 by ORF, into the vector pUC19 led to complementation of the panB mutant of Escherichia coli. The BCG pang gene was overexpressed in E. coli and the KPHMT purified to homogeneity The recombinant protein was further confirmed by an enzymatic assay.

• Molecules and Cells
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• v.42 no.2
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• pp.175-182
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• 2019

microRNAs regulate a diverse spectrum of cancer biology, including tumorigenesis, metastasis, stemness, and drug resistance. To investigate miRNA-mediated regulation of drug resistance, we characterized the resistant cell lines to 5-fluorouracil by inducing stable expression of miRNAs using lenti-miRNA library. Here, we demonstrate miR-551a as a novel factor regulating cell survival after 5-FU treatment. miR-551a-expressing cells (Hep3B-lenti-miR-551a) were resistant to 5-FU-induced cell death, and after 5-FU treatment, and showed significant increases in cell viability, cell survival, and sphere formation. It was further shown that myocyte-specific factor 2C is the direct target of miR-551a. Our results suggest that miR-551a plays a novel function in regulating 5-FU-induced cell death, and targeting miR-551a might be helpful to sensitize cells to anti-cancer drugs.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.12C
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• pp.1182-1191
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• 2003

In this paper, we propose a new hardware architecture for integer transform, quantizer, inverse quantizer, and inverse integer transform of a new video coding standard H.264/JVT. We describe the algorithm and derive hardware architecture emphasizing the importance of area for low cost and low power consumption. The proposed architecture has been verified by PCI-interfaced emulation board using APEX-II Alters FPGA and also by ASIC synthesis using Samsung 0.18 um CMOS cell library. The ASIC synthesis result shows that the proposed hardware can operate at 100 MHz, processing more than 1,300 QCIF video frames per second. The hardware is going to be used as a core module when implementing a complete H.264 video encoder/decoder ASIC for real-time multimedia application.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.43 no.9 s.351
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• pp.38-43
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• 2006

In this paper, we propose the efficient dead pixel detection algorithm for CMOS image sensors and its hardware architecture. The CMOS image sensors as image input devices are becoming popular due to the demand for miniaturized, low-power and cost-effective imaging systems. However, the presence of the dead pixels degrade the image quality. To detect the dead pixels, the proposed algorithm is composed of scan, trace and detection step. The experimental results showed that it could detect 99.99% of dead pixels. It was designed in a hardware description language and total logic gate count is 3.2k using 0.25 CMOS standard cell library.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.39 no.10
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• pp.11-20
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• 2002

This paper proposes a hardware/software codesign of the MLSE equalizer for GSM.GPRS systems. We analyze algorithms of the MLSE equalizer which consists of a channel estimator using the correlation method and the Viterbi processor. We estimate the computational complexity requirement based on the simulation of TI TMS320C5x DSP. We also estimate the gate count from the results of logic synthesis using the samsung 0.5㎛ standard cell library (STD80). Based on the results of the complexity estimation and gate count, we propose the efficient software/hardware codesign of the MLSE equalizer based on the results of the complexity estimation and gate count.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.8
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• pp.1283-1291
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• 2002

In this paper, the PLL driven by the DDFS is designed on the schematic using the Q-logic cell based library and is implemented using FPGA QL32 x16B. The measurement results of the frequency synthesizer switching speed were agreement with a register. The simulated results show that the clock delay was generated after eleven clock and if input is random, It has influence on output DA converter has to be very extensive. Therefore, the DDFS used noise shaper to drive PLL by regular interval for input state. Also the bandwidth of DA converter very extensive, the simulation shows that the variation of small input control word is better than the switching speed of PLL.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.11C
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• pp.1052-1057
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• 2006

The implement the adaptive partial response maximum likelihood (PRML) detector with tilt analyzer for asymmetric high-density optical storage system. For the estimation of disc tilt, we exploit spc patterns in each data frame. Because of using the ROM table to renew the coefficients of equalizer and reference values of branches, the complexity of the hardware is reduced. The proposed PRML has been designed and verified by VerilogHDL and synthesized by the Synopsys Design Compiler with Hynix $0.35{\mu}m$ STD cell library. In the result, the total gate count is 35K, and the maximum operating frequency is 140MHz.

• Molecular & Cellular Toxicology
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• v.2 no.3
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• pp.170-175
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• 2006

The extensive isolation of genes responsive to stressful conditions from a soft coral Scleronephthya gracillimum was described. Soft coral colonies were exposed to thermal and chemical stressors to induce the expression of stress related genes. Differentially expressed genes by natural or anthropogenic stressors were identified by construction of standard and stress exposed-paired subtractive cDNA library. Thirty-two and thirty-seven kinds of candidate genes were identified from thermal or benzo[a]pyrene stress exposed group, respectively, which are associated with cell cycle, cell signaling, transcription, translation, protein metabolism, and other cellular functions. The expected function of each gene was described. The isolated and identified differentially expressed genes have a great potential to identify environmental stressors in global environmental changes and could act as molecular biomarkers for biological responses against environmental changes. Finally, it may open a new paradigm on soft coral health assessment.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.7C
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• pp.704-711
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• 2002

This paper proposes a DFE (Decision Feedback Equalizer) equalizer ASIC using the Multi-Modulus Algorithm (MMA) for cable modem applications. We believe that it is the first effort to combine the DFE structure and the MMA algorithm. The proposed equalizer has been designed for 64/256 QAM modems. The existing MMA equalizer uses two transversal filters and updates two tap weights while the proposed equalizer uses two DFE filter banks to improve the channel adaptive performance and to reduce the number of taps and updates only one tap weights. We have used the 0.35 $\mu\textrm{m}$ standard cell library. The implemented equalizer ASIC operates at 8 MHz and provides 64 Mbps which is higher than existing equalizers. The total number of gates are about 160,000.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1607-1615
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• 2007

We investigated the applicability of the TEM-l ${\beta}$-lactamase fragment complementation (BFC) system to develop a strategy for the screening of protein-protein interactions in bacteria. A BFC system containing a human Fas-associated death domain (hFADD) and human Fas death domain (hFasDD) was generated. The hFADD-hFasDD interaction was verified by cell survivability in ampicillin-containing medium and the colorimetric change of nitrocefin. It was also confirmed by His pull-down assay using cell lysates obtained in selection steps. A coiled-coil helix coiled-coil domain-containing protein 5 (CHCH5) was identified as an interacting protein of human uracil DNA glycosylase (hUNG) from the bacterial BFC cDNA library strategy. The interaction between hUNG and CHCH5 was further confirmed with immunoprecipitation using a mammalian expression system. CHCH5 enhanced the DNA glycosylase activity of hUNG to remove uracil from DNA duplexes containing a U/G mismatch pair. These results suggest that the bacterial BFC cDNA library strategy can be effectively used to identify interacting protein pairs.