• 한국통신학회논문지
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• 제36권10A호
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• pp.809-815
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• 2011

본 논문에서는 고속 RS(Reed-Solomon) 복호기의 KES(Key Equation Solver) 블록 구현에 ME(Modified Euclidean) 알고리즘을 효율적으로 설계할 수 있는 구조를 제안하고 구현하였다. 제안된 구조에서는 각 PE(Processing Element) 블록을 제어하기 위해 새로운 상대변수를 정의하고 다항식으로 표현함으로써, 입출력 신호가 간단해지고, 차수계산회로가 필요 없기 때문에 회로의 복잡도를 줄일 수 있다. 또한, PE 회로가 오류 정정 능력 t와 무관하기 때문에, t가 증가함에 따라 KES 블록의 하드웨어 복잡도가 선형적으로 증가하는 장점을 가진다. 제안된 구조와 기존의 구조를 비교하기 위해, RS(255,239,8) 복호기에 대한 KES 블록을 구현하고, 0.13um CMOS cell library를 이용하여 합성하였다. 실험 결과로부터, 제안된 구조를 이용하여 적은 gate count로 고속 RS 복호기 구현이 가능함을 알 수 있다.

• 한국통신학회논문지
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• 제29권11C호
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• pp.1541-1550
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• 2004

본 논문에서는 다해상도 움직임 추정 알고리즘을 이용하여 모션 리터를 검색하는 고속 다해상도 움직임 추정기에 대한 하드웨어 구조를 제안한다. 동영상 압축기술인 MPEG-4 AVC 전체 구성 중에서 핵심 부분인 움직임 추정 모듈을 하드웨어로 설계하기 위하여 기본적인 구조를 구성하고 높은 화질로 실시간 부호화를 할 수 있도록 고속 움직임 검색을 위해 특수하게 설계된 램 구주 메모리 공유, 4화소x4화소 Motion Vector 추출 등과 같은 기술들을 사용하여 전체 움직임 검색기를 구현하였다. 구현된 전체 모듈은 Altera(사)의 Excalibur 디바이스를 이용한 FPGA 구성을 통해 검증하고 최종적으로 Samsung STD130 0.18um CMOS Cell Library를 이용하며 합성 및 검증을 하였다. 이렇게 검증된 구조의 성능은 ASIC으로 구현할 경우 최대 동작 주파수가 약 140MHz이며 QCIF(176화소x144화소) 사이즈 기준으로 초당 약 1100프레임, 4CIF(704화소x576화구 사이즈 기준으로 초당 약 70프레임의 움직임을 검색할 수 있다 본 성능은 하드웨어 기반의 MPEG-4 AVC 실시간 부호화기를 설계하기에 적합한 구조임을 보여준다.

• Genomics & Informatics
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• 제19권4호
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• pp.39.1-39.8
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• 2021

Tamoxifen (TAM) is an anticancer drug used to treat estrogen receptor (ER)-positive breast cancer. However, its ER-independent cytotoxic and antifungal activities have prompted debates on its mechanism of action. To achieve a better understanding of the ER-independent antifungal action mechanisms of TAM, we systematically identified TAM-sensitive genes through microarray screening of the heterozygous gene deletion library in fission yeast (Schizosaccharomyces pombe). Secondary confirmation was followed by a spotting assay, finally yielding 13 TAM-sensitive genes under the drug-induced haploinsufficient condition. For these 13 TAM-sensitive genes, we conducted a comparative analysis of their Gene Ontology (GO) 'biological process' terms identified from other genome-wide screenings of the budding yeast deletion library and the MCF7 breast cancer cell line. Several TAM-sensitive genes overlapped between the yeast strains and MCF7 in GO terms including 'cell cycle' (cdc2, rik1, pas1, and leo1), 'signaling' (sck2, oga1, and cki3), and 'vesicle-mediated transport' (SPCC126.08c, vps54, sec72, and tvp15), suggesting their roles in the ER-independent cytotoxic effects of TAM. We recently reported that the cki3 gene with the 'signaling' GO term was related to the ER-independent antifungal action mechanisms of TAM in yeast. In this study, we report that haploinsufficiency of the essential vps54 gene, which encodes the GARP complex subunit, significantly aggravated TAM sensitivity and led to an enlarged vesicle structure in comparison with the SP286 control strain. These results strongly suggest that the vesicle-mediated transport process might be another action mechanism of the ER-independent antifungal or cytotoxic effects of TAM.

• 약학회지
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• 제50권1호
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• pp.52-57
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• 2006

The activation of cyclin dependent kinase 4 (CDK4) is found in more than half of all human cancers. Therefore CDK4 is an attractive target for the development of a novel anticancer agent. For mass screening of CDK4 inhibitor, we set up in vitro kinase assay for CDK4 activity using a cyclin D1-CDK4 fusion protein, which is constitutively active and exhibits enhanced stability. From the screening of representative compound library of Korea Chemical Bank, we found that 7-chloro-4-nitro-benzo[1,2,5]oxadiazole 1-oxide (FBP-1248) selectively inhibited CDK4 activity in vitro by ATP competitive manner. This compound prevented the phosphorylation of retinoblatsoma tumor suppressor protein, Rb, and inhibited cell growth through cell cycle arrest. In summary, we developed an efficient assay system for CDK4 activity in vitro and identified the CDK4 inhibitory compound, FBP-1248.

• 대한의생명과학회지
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• 제10권3호
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• pp.211-217
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• 2004

For discrimination of ductal and ascinar cells, we isolated a single-chain variable domain fragment (scFv) antibody against ductal cells of salivary gland using phage display technique. From the spleen of a mouse immunized with ductal cell lysate, total RNA was prepared and used as a template for cDNA synthesis of antibody genes. The scFv genes were constructed with variable domain genes of heavy and light chain and were introduced into pCANTAB5E to construct phage scFv library. The phage particles specific for acinar cells were screened by subtraction using immunotubes coated with acinar and ductal cell lysate and enzyme-linked immunoabsorbance assay (ELISA). The characteristics of the scFv were determined by immunohistochemistry (IHC) and the result indicated that the isolated scFv has the specificity against ductal cells of salivary glands and tubules of kidney. And the scFv has an unique binding activity specific for Hashimoto's thyroiditis. The nucleotide sequence of isolated scFv gene was determined and revealed that V/sub H/ belongs to the mouse H-chain family subgroup IB and V/sub L/ to the mouse L-chain family subgroup III.

• BMB Reports
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• 제34권6호
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• pp.526-530
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• 2001

The translationally controlled tumor protein (TCTP), also known as the IgE-dependent histamine releasing factor (HRF), was used in the yeast two-hybrid system to screen the interacting molecules. We obtained the N-terminus truncated rat fast myosin alkai light chain from the rat skeletal muscle cDNA library in the screening. Since either TCTP/HRF or the myosin light chain is known to be associated with histamine secretion from RBL-2H3 cells, we investigated the possible interaction between rat TCTP/HRF and nonmuscle myosin light chain in these cells. We used affinity chromatography and coimmunoprecipitation. Our data suggests that HRF and the myosin light chain interact, which may play an important role in histamine release in RBL-2H3 cells.

• Biomolecules & Therapeutics
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• 제26권5호
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• pp.458-463
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• 2018

The phosphorylation of JNK is known to induce insulin resistance in insulin target tissues. The inhibition of JNK-JIP1 interaction, which interferes JNK phosphorylation, becomes a potential target for drug development of type 2 diabetes. To discover the inhibitors of JNK-JIP1 interaction, we screened out 30 candidates from 4320 compound library with In Cell Interaction Trap method. The candidates were further confirmed and narrowed down to five compounds using the FRET method in a model cell. Among those five compounds, Acebutolol showed notable inhibition of JNK phosphorylation and elevation of glucose uptake in diabetic models of adipocyte and liver cell. Structural computation showed that the binding affinity of Acebutolol on the JNK-JIP1 interaction site was comparable to the known inhibitor, BI-78D3. Our results suggest that Acebutolol, an FDA-approved beta blocker for hypertension therapy, could have a new repurposed effect on type 2 diabetes elevating glucose uptake process by inhibiting JNK-JIP1 interaction.

• 한국전자파학회논문지
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• 제13권8호
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• pp.764-768
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• 2002

본 논문에서는 (주)타키오닉스의 SiGe HBT 공정을 이용하여 double balanced Gilbert cell down conversion MMIC mixer를 구현한 결과를 제시하고, 이를 통해 RFIC 설계용 SiGe HBT 파운드리의 정확성과 신뢰성을 평가하였다. 제작된 Mixer는 3 V 동작 전압에서 10 mA의 전류를 소모하며, 2 GHz의 주파수 대역에서 17 dB의 Conversion Gain과 9.8 dB NF, -4.2 dBm Output 1 dB Compression Point, -27 dEc의 RF-IF Isolation의 특성을 나타내었으며 우수한 50 $\Omega$ 입. 출력 정합 특성을 갖는다. 시뮬레이션 결과와 측정결과는 거의 유사한 특성을 가지며, 이를 통해 SiGe HBT 모델 라이브러리의 정확성과 공정의 재현성을 검증할 수 있었다.

• BMB Reports
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• 제52권9호
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• pp.540-547
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• 2019

Immune repertoire is a collection of enormously diverse adaptive immune cells within an individual. As the repertoire shapes and represents immunological conditions, identification of clones and characterization of diversity are critical for understanding how to protect ourselves against various illness such as infectious diseases and cancers. Over the past several years, fast growing technologies for high throughput sequencing have facilitated rapid advancement of repertoire research, enabling us to observe the diversity of repertoire at an unprecedented level. Here, we focus on B cell receptor (BCR) repertoire and review approaches to B cell isolation and sequencing library construction. These experiments should be carefully designed according to BCR regions to be interrogated, such as heavy chain full length, complementarity determining regions, and isotypes. We also highlight preprocessing steps to remove sequencing and PCR errors with unique molecular index and bioinformatics techniques. Due to the nature of massive sequence variation in BCR, caution is warranted when interpreting repertoire diversity from error-prone sequencing data. Furthermore, we provide a summary of statistical frameworks and bioinformatics tools for clonal evolution and diversity. Finally, we discuss limitations of current BCR-seq technologies and future perspectives on advances in repertoire sequencing.

• Journal of Microbiology and Biotechnology
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• 제32권12호
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• pp.1615-1621
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• 2022

Tissue regeneration is the ultimate treatment for many degenerative diseases, however, repair and regeneration of damaged organs or tissues remains a challenge. Previously, we showed that B1 Ab and H3 Ab induce stem cells to differentiate into microglia and brown adipocyte-like cells, while trafficking to the brain and heart, respectively. Here, we present data showing that another selected agonist antibody, P1 antibody, induces the migration of cells to the pancreatic islets and differentiates human stem cells into beta-like cells. Interestingly, our results suggest the purified P1 Ab induces beta-like cells from fresh, human CD34⁺ hematopoietic stem cells and mouse bone marrow. In addition, stem cells with P1 Ab bound to expressed periostin (POSTN), an extracellular matrix protein that regulates tissue remodeling, selectively migrate to mouse pancreatic islets. Thus, these results confirm that our in vivo selection system can be used to identify antibodies from our library which are capable of inducing stem cell differentiation and cell migration to select tissues for the purpose of regenerating and remodeling damaged organ systems.