• Molecules and Cells
• /
• v.37 no.12
• /
• pp.841-850
• /
• 2014

Polycomb group (PcG) proteins are conserved chromatin regulators involved in the control of key developmental programs in eukaryotes. They collectively provide the transcriptional memory unique to each cell identity by maintaining transcriptional states of developmental genes. PcG proteins form multi-protein complexes, known as Polycomb repressive complex 1 (PRC1) and Polycomb repressive complex 2 (PRC2). PRC1 and PRC2 contribute to the stable gene silencing in part through catalyzing covalent histone modifications. Components of PRC1 and PRC2 are well conserved from plants to animals. PcG-mediated gene silencing has been extensively investigated in efforts to understand molecular mechanisms underlying developmental programs in eukaryotes. Here, we describe our current knowledge on PcG-mediated gene repression which dictates developmental programs by dynamic layers of regulatory activities, with an emphasis given to the model plant Arabidopsis thaliana.

• International Journal of Internet, Broadcasting and Communication
• /
• v.10 no.1
• /
• pp.16-22
• /
• 2018

This paper presents a solution for personal profiling system based on user-oriented tracking. Here, we introduce a new way to identify and track humans by using two types of cameras: dome and face camera. Dome camera has a wide view angle so that it is suitable for tracking human movement in large area. However, it is difficult to identify a person only by using dome camera because it only sees the target from above. Thus, face camera is employed to obtain facial information for identifying a person. In addition, we also propose a new mechanism to locate human on targeted location by using grid-cell system. These result in a system which has the capability of maintaining human identity and tracking human activity (movement) effectively.

• Journal of Integrative Natural Science
• /
• v.10 no.1
• /
• pp.20-26
• /
• 2017

Chemerin receptor, which predominantly expressed in immune cells as well as adipose tissue, was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. Chemerin is a widely distributed multifunctional secreted protein implicated in immune cell migration, adipogenesis, osteoblastogenesis, angiogenesis, myogenesis, and glucose homeostasis. Recent studies suggest chemerin may play an important role in the pathogenesis of obesity and insulin resistance and it becomes a potential therapeutic target for treating type II diabetes. The crystal structure of chemerin receptor has not yet been resolved. Therefore, in the present study, homology modelling of CMKLR1 was done utilizing the crystal structure of human angiotension receptor in complex with inverse agonist olmesartan as the template. Since the template has low sequence identity, we have incorporated both threading and comparative modelling approach to generate the three dimensional structure. 3D models were generated and validated. The reported models can be used to characterize the critical amino acid residues in the binding site of CMKLR1.

• 한국정보통신설비학회:학술대회논문집
• /
• 2008.08a
• /
• pp.229-232
• /
• 2008

급격한 이동통신 기술의 발전에 힘입어 음영지역 해소 및 고속 데이터 처리를 위해 댁내 기지국에 대한 개발 및 연구가 진행 중이다. 댁내 기지국은 크게 음영지역 해소를 위한 Open 방식의 기지국과 고속 데이터 처리를 위해 특정 가입자만 사용할 수 있는 Close 방식이 있다. 상기 방식들은 망의 특성에 맞게 이를 제공하는 망 사업자에 의해 선택 된다. 댁내 기지국을 관리하기 위해서는 많은 시간과 인력자원이 소요되므로 자동으로 설정 및 최적화시키는 기능이 요구 시 되고 있으며, 이를 3GPP에서 SON (Self Organizing Optimizing Networks) 이라 일컫고 연구진행 중이다. 본 논문은 댁내 기지국 관리를 위해 셀의 기본 인자인 PCI(Physical Layer Identity) 할당 방안과 댁내 기지국간 간섭을 최소화 시키기 위한 Adaptive Coverage 방안을 제시한다. 또한 계층적 셀 구성(Hierarchical Cell Structure)에 따른 이동성 제공 방안을 제시한다.

• Molecules and Cells
• /
• v.44 no.6
• /
• pp.377-383
• /
• 2021

Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is a novel virus that causes coronavirus disease 2019 (COVID-19). To understand the identity, functional characteristics and therapeutic targets of the virus and the diseases, appropriate infection models that recapitulate the in vivo pathophysiology of the viral infection are necessary. This article reviews the various infection models, including Vero cells, human cell lines, organoids, and animal models, and discusses their advantages and disadvantages. This knowledge will be helpful for establishing an efficient system for defense against emerging infectious diseases.

• BMB Reports
• /
• v.56 no.12
• /
• pp.633-644
• /
• 2023

Epigenetic mechanisms, primarily mediated through histone and DNA modifications, play a pivotal role in orchestrating the functional identity of a cell and its response to environmental cues. Similarly, the spatial arrangement of chromatin within the three-dimensional (3D) nucleus has been recognized as a significant factor influencing genomic function. Investigating the relationship between epigenetic regulation and 3D chromatin structure has revealed correlation and causality between these processes, from the global alignment of average chromatin structure with chromatin marks to the nuanced correlations at smaller scales. This review aims to dissect the biological significance and the interplay between the epigenome and 3D chromatin structure, while also exploring the underlying molecular mechanisms. By synthesizing insights from both experimental and modeling perspectives, we seek to provide a comprehensive understanding of cellular functions.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.5
• /
• pp.606-612
• /
• 2012

Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.

• Journal of Veterinary Clinics
• /
• v.22 no.3
• /
• pp.244-248
• /
• 2005

This study was conducted to survey the relationship between the somatic cell count (SCC), and California mastitis test (CMT) & mastitis. A total of 328 quarter milk samples were collected from 211 cows suspected to have mastitis; Both SCC and CMT were performed on the samples. Milk smear was stained with Broadhurst and Paley stain and the cells were classified into either epithelial or blood cells. Bacterial isolation was made and antimicrobial susceptibility was tested. Of the 328 quarters, 78 ($23.8{\%}$) were CMT negative with SCC <750,000/ml. As expected, CMT score increased with the increase of SCC. The number of epithelial cells decreased with the increasing number of somatic cells, while the opposite was the case with the number of blood cells. The critical point was when the SCC reached 1,000,000/ml. Up to 1,000,000 cells/ml, the number of epithelial cells was greater than that of blood cells. The results indicate that when epithelia:blood cell ratio is 58.1:41.9, the milking line should be checked and bacterial isolation be performed on the samples in order to identity mastitis.

• Archives of design research
• /
• v.19 no.4 s.66
• /
• pp.175-186
• /
• 2006

In the 21C, each nation controls exhaust fumes from automobiles and makes an effort to develop alternative energy because of serious environmental problem. Jechon area has many historical and cultural archeological sites. And Jechon city sponsors various cultural events. But the way of transportation which is connected with Jecheon and around sightseeing places is general and not ready yet. Therefore, if a special means of vehicle is developed, it could play an another role of sightseeing resources. Special identity of Jecheon area for establishment of green vehicle traffic system which gives Jecheon area specific character was investigated for theoretical background. Traffic system was studied for establishment of direction through existent successful case study. Moreover content, method, structure and advantage & shortcoming etc. of vehicle that use green energy resource such as solar car, fuel cell car, hybrid car, natural gas car etc. were examined. The suitable means of vehicle for Jechon area was proposed to three directions with research and investigation. After comparison and investigation by inquiry of each section's experts, the most suitable traffic system of which energy resource of car, form of vehicles, the complement, dimension of vehicles etc. were decided. Design proposal should be drawn according to process of automobile design in decided direction. Special Exterior design of vehicle that use green energy resource connecting Jecheon and around area should be suggested in Jecheon City Hall and Chungchong-bukdo provincial office for vivify image of cleanliness area.

• The Korean Society of Law and Medicine
• /
• v.22 no.4
• /
• pp.159-184
• /
• 2021

The significance of the enactment of the 「Act On The Safety Of And Support For Advanced Regenerative Medicine And Advanced Biological Products」 is to break away from the regulation of the Pharmaceutical Affairs Act and expand patient treatment opportunities through a medical technology approach to regenerative medicine, which is essentially a medical practice called 'transplantation'. However, more than a year after the law was enacted, clinical study has not been activated, with not a single high-risk study approved by the Ministry of Food and Drug Safety being approved. The reason is that despite the legal purpose of expanding patient treatment opportunities, the data requirements for clinical study approval are set in connection with drug development despite the insufficient legal basis, making it difficult for many researchers to meet the data requirements. Prior to the enactment of the Act, submitted data for clinical study on cell therapy products within the Pharmaceutical Affairs Act were cosiderably exempted from quality and non-clinical test data, but with the enforcement of the Advanced Regenerative Bio Act, quality and non-clinical test data are required in accordance with pharmaceuticals when applying for approval of a clinical study plan. To rectify this, when considering the identity of clinical study on advanced regenerative medicine to expand treatment opportunities, recognize that there are limitations in connection with drug development. And it is necessary to preserve the identity of clinical study on advanced regenerative medicine, and on the other hand, in the case of drug product approval, clinical study results should be utilized while specifying usage requirements. Therefore, with the power of the market and the voluntary motive of the clinical researcher, it is necessary to prepare the necessary data by themselves rather than the basic requirements for clinical study approval.