• BMB Reports
• /
• v.49 no.10
• /
• pp.527-528
• /
• 2016

In embryonic stem cells (ESCs), cell cycle regulation is deeply connected to pluripotency. Especially, core transcription factors (CTFs) which are essential to maintaining the pluripotency transcription programs should be reset during M/G1 transition. However, it remains unknown about how CTFs are governed during cell cycle progression. Here, we describe that the regulation of Oct4 by Aurora kinase b (Aurkb)/protein phosphatase 1 (PP1) axis during the cell cycle is important for resetting Oct4 to pluripotency and cell cycle related target genes in determining the identity of ESCs. Aurkb starts to phosphorylate Oct4(S229) at the onset of G2/M phase, inducing the dissociation of Oct4 from chromatin, whereas PP1 binds Oct4 and dephosphorylates Oct4(S229) during M/G1 transition, which resets Oct4-driven transcription for pluripotency and the cell cycle. Furthermore, Aurkb phosphormimetic and PP1 binding-deficient mutations in Oct4 disrupt the pluripotent cell cycle, lead to the loss of pluripotency in ESCs, and decrease the efficiency of somatic cell reprogramming. Based on our findings, we suggest that the cell cycle is directly linked to pluripotency programs in ESCs.

• Animal cells and systems
• /
• v.6 no.1
• /
• pp.1-12
• /
• 2002

A role of Src family protein Tyrosine kinases (SFK) as mediators of receptor-ligand initiated responses is well established. Well documented, but less well understood is the role of SFK in cellular reaction to stresses. Evidence from the wide variety of experimental systems indicates that SFK mediate responses to all major classes of stress, including oxidation, DNA damage, mechanical impacts, and protein denaturing. SFK may be activated by stresses directly or via regulatory circuits whose identity is not yet fully understood. Depending on the cell type and the nature of activating stimulus, SFK may activate known downstream signaling cascades leading to cell survival, proliferation, cytoskeletal rearrangement, and apoptosis; the identity of these cascades is discussed. As in the case of receptor-initiated signaling, roles of individual SFK in various stress response may be redundant or non-redundant. Although signals generated by different stresses are generally transduced via distinct SFK pathways, these pathways may overlap or exhibit crosstalk. In some cell types stress-induced activation of SFK promotes survival and inhibits apoptosis, whereas the opposite may be true for other cell types. Stress responses constitute a new and rapidly developing area of SFK-mediated signaling.

• Journal of Information Processing Systems
• /
• v.5 no.4
• /
• pp.207-220
• /
• 2009

A large number of phone calls and data services will take place in indoor environments. In Long Term Evolution (LTE), femtocell, as a home base station for indoor coverage extension and wideband data service, has recently gained significant interests from operators and consumers. Since femtocell is frequently turned on and off by a personal owner, not by a network operator, one of the key issues is that femtocell should be identified autonomously without system information to support handover from macrocell to femtocell. In this paper, we propose a dynamic reservation scheme of Physical Cell Identities (PCI) for 3GPP LTE femtocell systems. There are several reserving types, and each type reserves a different number of PCIs for femtocell. The transition among the types depends on the deployed number of femtocells, or the number of PCI confusion events. Accordingly, flexible use of PCIs can decrease PCI confusion. This reduces searching time for femtocell, and it is helpful for the quick handover from macrocell to femtocell. Simulation results show that our proposed scheme reduces average delay for identifying detected cells, and increases network capacity within equal delay constraints.

• Mass Spectrometry Letters
• /
• v.14 no.4
• /
• pp.121-140
• /
• 2023

It is becoming more and more clear that each cell, even those of the same type, has a unique identity. This sophistication and the diversity of cell types in tissue are what are pushing the necessity for spatially distributed omics at the single-cell (SC) level. Single-cell chemical assessment, which also provides considerable insight into biological, clinical, pharmacodynamic, pathological, and toxicity studies, is crucial to the investigation of cellular omics (genomics, metabolomics, etc.). Mass spectrometry (MS) as a tool to image and profile single cells and subcellular organelles facilitates novel technical expertise for biochemical and biomedical research, such as assessing the intracellular distribution of drugs and the biochemical diversity of cellular populations. It has been illustrated that ambient mass spectrometry (AMS) is a valuable tool for the rapid, straightforward, and simple analysis of cellular and sub-cellular constituents and metabolites in their native state. This short review examines the advances in ambient mass spectrometry (AMS) and ambient mass spectrometry imaging (AMSI) on single-cell analysis that have been authored in recent years. The discussion also touches on typical single-cell AMS assessments and implementations.

• Molecules and Cells
• /
• v.28 no.1
• /
• pp.7-12
• /
• 2009

Gliomas, much like other cancers, are composed of a heterogeneous mix of neoplastic and non-neoplastic cells that include both native and recruited cells. There is extensive diversity among the tumor cells, with differing capacity for In vitro and in vivo growth, a property intimately linked to the cell's differentiation status. Those cells that are undifferentiated, self-renewing, with the capacity for developing tumors (tumorigenic) cells are designated by some as cancer stem cells, because of the stem-like properties. These cells may be a critical therapeutic target. However the exact identity and cell(s) of origin of the socalled glioma cancer stem cell remain elusive. Here we review the current understanding of glioma cancer stem cell biology.

• Proceedings of the Korean Society of Broadcast Engineers Conference
• /
• 2020.07a
• /
• pp.593-595
• /
• 2020

본 논문에서는 5G NR 시스템을 위한 동기 신호를 이용한 cell ID 검출 방법에 대한 성능을 비교하였다. 5G NR(fifth-generation new radio) 시스템의 송신기는 SS/PBCH (synchronization signal/physical broadcast channel) 블록을 송신하며, 수신기는 수신된 SS/PBCH 블록을 이용하여 주파수 및 타이밍 오프셋 (frequency and timing offset)을 추정 할 수 있으며, cell ID (cell identity)는 PSS (primary synchronization signal)와 SSS (secondary synchronization signal)를 통해 검출할 수 있다. 본 논문에서는 cell ID 를 검출할 수 있는 방법으로서 2-stage 디코딩 방법과 결합 최대우도 결정 규칙 (joint maximum-likelihood decision rule: joint ML) 디코딩 방법을 사용하였다. Joint ML 디코딩 방법은 2-stage 디코딩 방법에 비해 더 좋은 검출 성능을 보이지만, 복잡도 측면에서는 2-stage 디코딩 방법이 joint ML 디코딩 방법에 비해 더 낮은 복잡도를 갖는 것을 확인하였다.

• Proceedings of the Korean Society of Broadcast Engineers Conference
• /
• 2020.07a
• /
• pp.634-636
• /
• 2020

본 논문에서는 딥러닝 (deep learning) 방식을 이용한 5G NR (fifth-generation new radio)의 cell ID (cell identity) 검출 기법을 구현하였다. 5G NR 시스템의 단말 (user equipment)은 초기 접속 (initial access)과정에서 PSS (primary synchronization signal)와 SSS (secondary synchronization signal)을 이용한 동기 획득 및 cell ID 검출이 필요하다. 본 논문에서는 분류 기법 기반의 딥러닝 기술을 이용하여 인공 신경망 모델에 PSS 및 SSS 와 cell ID 의 상관 관계를 학습시키고, 학습된 모델의 성능을 제시하였다.

• Genomics & Informatics
• /
• v.20 no.1
• /
• pp.2.1-2.11
• /
• 2022

The method of single-cell RNA sequencing has been rapidly developed, and numerous experiments have been conducted over the past decade. Their results allow us to recognize various subpopulations and rare cell states in tissues, tumors, and immune systems that are previously unidentified, and guide us to understand fundamental biological processes that determine cell identity based on single-cell gene expression profiles. However, it is still challenging to understand the principle of comprehensive gene regulation that determines the cell fate only with transcriptome, a consequential output of the gene expression program. To elucidate the mechanisms related to the origin and maintenance of comprehensive single-cell transcriptome, we require a corresponding single-cell epigenome, which is a differentiated information of each cell with an identical genome. This review deals with the current development of single-cell epigenomic library construction methods, including multi-omics tools with crucial factors and additional requirements in the future focusing on DNA methylation, chromatin accessibility, and histone post-translational modifications. The study of cellular differentiation and the disease occurrence at a single-cell level has taken the first step with single-cell transcriptome and is now taking the next step with single-cell epigenome.

• BMB Reports
• /
• v.48 no.4
• /
• pp.193-199
• /
• 2015

Degenerative retinal diseases affect millions of people worldwide, which can lead to the loss of vision. However, therapeutic approaches that can reverse this process are limited. Recent efforts have allowed the possibility of the stem cell-based regeneration of retinal cells and repair of injured retinal tissues. Although the direct differentiation of pluripotent stem cells into terminally differentiated photoreceptor cells comprises one approach, a series of studies revealed the intrinsic regenerative potential of the retina using endogenous retinal stem cells. Muller glial cells, ciliary pigment epithelial cells, and retinal pigment epithelial cells are candidates for such retinal stem cells that can differentiate into multiple types of retinal cells and be integrated into injured or developing retina. In this review, we explore our current understanding of the cellular identity of these candidate retinal stem cells and their therapeutic potential for cell therapy against degenerative retinal diseases. [BMB Reports 2015; 48(4): 193-199]

• Reproductive and Developmental Biology
• /
• v.36 no.3
• /
• pp.155-161
• /
• 2012

Germ cell-specific hyaluronidases such as sperm adhesion molecule 1 (SPAM1) and hyaluronoglucosaminidase 5 (Hyal5) are in part responsible for dispersal of the cumulus cell mass, which is a critical step in establishing fertilization in mammals. In this study, we identified two testis-hyaluronidases, SPAM1 and Hyal5, in hamster and rat. These two genes were expressed specifically in the testis. At the protein level, hamster SPAM1 and Hyal5 display 78.7% and 75.4% identity with mouse SPAM1 and Hyal5. Further, the activity of the enzymes with respect to cumulus cell dispersion did not differ, although we observed that the enzymatic activity differed in pH range. These studies suggest that different sperm hyaluronidases are capable of dispersing the cumulus cell mass despite differences in enzyme activity.