• 제목/요약/키워드: cause-effect pathway

검색결과 94건 처리시간 0.027초

Cyclopamine, an Antagonist of Hedgehog (Hh) Signaling Pathway, Reduces the Hatching Rate of Parthenogenetic Murine Embryos

  • Park, Jaehyun;Moon, Jeonghyeon;Min, Sol;Chae, Stephan;Roh, Sangho
    • 한국수정란이식학회지
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    • 제33권4호
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    • pp.237-243
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    • 2018
  • Hedgehog (Hh) pathway plays a key role in development from invertebrate to vertebrate. It is known to be involved in cell differentiation, polarity, proliferation, including the development of vertebrate limb and the establishment of flies' body plan. To investigate how the regulation of Hh pathway affects the development of parthenogenetic murine embryos, the parthenogenetically activated murine embryos were treated with either cyclopamine (Cyc), an antagonist of Hh pathway, or purmorphamine, an agonist of Hh pathway. While Cyc did not affect the blastocyst formation and its total cell number, the chemical reduced the hatching rate of embryos and the expression levels of Fn1 mRNA. The results of the present study show the possibility that Cyc may affect the development of embryos at blastocyst stage by blocking Hh pathway and this may cause detrimental effect to the embryos at peri-, and post-implantation stages.

자궁적출술 환자를 위한 critical pathway 개발과 적용효과 (Critical Pathway Development for the Hysterectomy Patients and its applied Effect)

  • 노기옥;박경숙
    • 여성건강간호학회지
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    • 제6권2호
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    • pp.234-257
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    • 2000
  • At present in the medical care, the study and effort for producing health service to consider efficiency, effectiveness, and quality are urgently called for because of the difficulty in the keen competition according to the inter- nationalization and opening, the operation in the medical institution service testing system, the change in the medical policy of KDRGs, and the lack of the health care cost increasing rate. As an alternative, the case management for the new management system is introduced in the U.S., and the Critical Pathway that is the method designing the contents of activity and its result has been developed and applied in order to anticipate and manage the patient-outcome for the realization of the cost-effective case-management. Thus, this study intended to analyze the effectiveness to obtain by developing the Critical Pathway presented as the method to improve the quality-betterment and cost effectiveness through the continuous and consistent patient management for the hysterectomy patient and applying it to the real practice. As a study method, this author formed a conceptual framework through considering five Critical Pathway used in the current U.S. and three Critical Pathway presented in the literature to develop the Critical Pathway for the hysterectomy patient, and made out the preliminary Critical Pathway through reviewing the old chart. This author made the verified the validity of the expert group about the developed Critical Pathway, and to confirm the possibility of practice application, completed and settled the final Critical Pathway after using the Critical Pathway to the hysterectomy patient from March 1st to 15th, 1997. Finally, to analyze the application-effect of the developed Critical Pathway, this author offered health care service applying the Critical Pathway to the hysterectomy patient from April 15th to August 31th, 1997. The guide for the Critical Pathway was carried out in advance by outpatient setting nurse for outpatient setting visit before the operation, and after hospitalization the primary nurse monitored the execution degree on the every duty. After discharge this author surveyed the complication through phone visiting, and one month after discharge surveyed the patient's reaction about the offered service when outpatient setting visit and analyzed the result. The source for health care cost was obtained by the statistics about the hospital charge which was offered by the General Business Department. The results were as follows. 1. It was decided that the vertical line of the Critical Pathway was made up of eight items such as monitoring/assessment, treatment, line/drains, activity, medication, lab test, diet, patient teaching, and the horizontal line of the Critical Pathway was made up of from hospitalization to discharge. 2. After the analysis of service contents through reviewing the old chart, it was decided that the horizontal line of the preliminary Critical Pathway was made up of from hopitalization to fourth postoperative day, and the vertical line of it was divided into eight items which were the contents to occur with the time frame of the horizontal line. 3. After the verifying the validity of the expert group about the preliminary Critical Pathway, the horizontal line was amended from hopitalization to third postoperative day, and taking their consensus, some contents of the horizontal line was amended and deleted. 4. From March 1st to 15th, 1997, to confirm the clinical suitability, this author offered eight hysterectomy patients the medical service through the Critical Pathway. The result was that three of them could be discharged at the expected discharge day, and the others later than that day. Supplementing the preliminary Critical Pathway through analyzing the cause of that delay- case, this author developed the final Critical Pathway. 5. There were no significant differences between the experimental and the control group in the incidence of complication(P > 0.05). 6. The 92.4% of experimental group was satisfied with the Critical Pathway service. 7. The length of hospital stay of the experimental group offered with the Critical Pathway service was 4.6 days and there was a significant difference that it was 1.3 days shorter than that of the control group(t=-29.514, P=0.000). 8. There wsa a significant difference that the mean medical charge per one patient of the experimental group offered the Critical Pathway service was cheaper \124,150 than that of the control group(t=-9.826, P=0.000). 9. The result that the author assumed and analyzed hospital income with the rate of turning bed was assumed that the increase of hospital income was \63,245,072 for that study, and the income increase was expected with \68,704,864 for a year. The result that this author applied the Critical Pathway to the hysterectomy patient have no differences in the incidence of complication, high satisfaction with that service, and the length of hospital stay decreased in the experimental group, and the mean hospital charge per one patient decreased, but hospital income increased. Suggestions for further study and nursing practice are as follows. 1. The study to apply the Critical Pathway for a year, verify the validity, and measure the effect repeatedly is needed. 2. To apply and manage the Critical Pathway effectively, the study to computerize it is needed. 3. The study to develop hospital-based Critical Pathway about other diseases or procedure, and measure the effect is needed.

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Effect of the hedgehog signaling pathway on hair formation-related cells

  • Park, Jaehyun;Park, Sangkyu;Seo, Jeongmin;Roh, Sangho
    • International Journal of Oral Biology
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    • 제44권4호
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    • pp.144-151
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    • 2019
  • Alopecia has emerged as one of the biggest interests in modern society. Many studies have focused on the treatment of alopecia, such as transplantation of hair follicles or inhibition of the androgen pathway. Hair growth is achieved through proper proliferation of the components such as keratinocytes and dermal papilla cells (DPCs), movement, and interaction between the two cells. The present study examined the effect of the hedgehog (Hh) signaling pathway, which is an important and fundamental signal in the cell, on the morphology and the viability of human keratinocytes and DPCs. Upregulation of Hh signaling caused a morphological change and an increase in epithelium-mesenchymal transition-related gene expression but reduced the viability of keratinocytes, while the alteration of Hh signaling did not cause any change in DPCs. The results show the possibility that the regulation of Hh signaling can be applied for the treatment of alopecia.

2-Methoxy-1,4-naphthoquinone (MNQ) regulates cancer key genes of MAPK, PI3K, and NF-κB pathways in Raji cells

  • Wong, Teck Yew;Menaga, Subramaniam;Huang, Chi-Ying F.;Ho, Siong Hock Anthony;Gan, Seng Chiew;Lim, Yang Mooi
    • Genomics & Informatics
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    • 제20권1호
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    • pp.7.1-7.13
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    • 2022
  • 2-Methoxy-1,4-naphthoquinone (MNQ) has been shown to cause cytotoxic towards various cancer cell lines. This study is designed to investigate the regulatory effect of MNQ on the key cancer genes in mitogen-activated protein kinase, phosphoinositide 3-kinase, and nuclear factor κB signaling pathways. The expression levels of the genes were compared at different time point using polymerase chain reaction arrays and Ingenuity Pathway Analysis was performed to identify gene networks that are most significant to key cancer genes. A total of 43 differentially expressed genes were identified with 21 up-regulated and 22 down-regulated genes. Up-regulated genes were involved in apoptosis, cell cycle and act as tumor suppressor while down-regulated genes were involved in anti-apoptosis, angiogenesis, cell cycle and act as transcription factor as well as proto-oncogenes. MNQ exhibited multiple regulatory effects on the cancer key genes that targeting at cell proliferation, cell differentiation, cell transformation, apoptosis, reduce inflammatory responses, inhibits angiogenesis and metastasis.

행위중독에 대한 사회적 낙인과정 - 귀인정서이론 적용 - (Process of Social Stigma on Behavioral Addictions -The Attribution Affection Theory applied -)

  • 박근우;서미경
    • 한국사회복지학
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    • 제69권3호
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    • pp.241-265
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    • 2017
  • 본 연구는 사회적 낙인이 행위중독의 치료를 방해하는 주요 요인이라는 전제하에 행위중독에 대한 사회적 낙인과정을 검증하여 반낙인 전략을 제안하기 위해 수행되었다. 연구모형은 정신장애에 대한 대중의 정서적, 행동적 반응을 설명하는 데 매우 유용하게 활용되는 귀인정서이론과 수정된 귀인정서이론을 적용하여 구성하였다. 즉, 행위중독의 원인을 성격이나 의지, 생활습관 등 개인적으로 통제 가능한 것으로 귀인할 경우 분노를 매개로 사회적 거리감이 증가될 수 있다. 그러나 행위중독의 원인을 개인이 통제할 수 없는 생물학적 요인으로 귀인할 경우 동정을 매개로 돕는 행동이 나타나거나(귀인정서이론), 오히려 위험하다는 인식으로 인해 두려움이 증가하여 사회적 거리감이 가중되는(수정된 귀인정서이론) 두 가지 경로가 존재할 수 있다. 연구모형 검증을 위해 경상남도의 성인 383명을 대상으로 도박중독과 인터넷 게임중독의 모의 사례를 무작위로 제시하고 사례가 보이는 문제의 원인, 그들에 대한 정서적, 행동적 반응을 질문하였다. 그 결과 모든 경로가 통계적으로 의미 있는 예측력을 가졌다. 따라서 개인적 원인으로 귀인할 경우 분노를 매개로 사회적 거리감이 증가하였다. 생물학적 원인으로 귀인할 경우 두 경로 모두 의미 있는 예측력을 가졌으나 동정을 매개로 돕는 행동을 예측하는 경로의 간접효과가 더 컸다. 이러한 결과에 따라 연구자들은 행위중독이 치료가 필요한 정신건강의 문제임을 강조하는 반낙인 전략을 제안하였다.

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Cytotoxic Effect of Triglycerides via Apoptotic Caspase Pathway in Immune and Non-immune Cell Lines

  • Lim, Jaewon;Yang, Eun Ju;Chang, Jeong Hyun
    • 대한의생명과학회지
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    • 제25권1호
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    • pp.66-74
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    • 2019
  • Hyperlipidemia is defined as conditions of the accumulation of lipids such as free fatty acids (FFA), triglyceride (TG), cholesterol and/or phospholipid in the bloodstream. Hyperlipidemia can cause lipid accumulation in non-adipose tissue, which is lipid-cytotoxic effects in many tissues and mediates cell dysfunction, inflammation or programmed cell death (PCD). TG is considered to be a major cause of atherosclerosis through inflammatory necrosis of vascular endothelial cells. Recently, TG have also been shown to exhibit lipid-cytotoxicity and induce PCD. Therefore, we investigated the effect of TG on the cytotoxic effect of various cell types. When exposed to TG, the cell viability of U937 monocytes and Jurkat T lymphocytes, as well as the cell viability of MCF-7, a non-immune cell, decreased in time- and dose-dependent manner. In U937 cells and Jurkat cells, caspase-9, an intrinsic apoptotic caspase, and caspase-8, an extrinsic apoptotic caspase, were increased by exposure to TG. However, in TG-treated MCF-7 cells, caspase-8 activity increased only without caspase-9 activity. In addition, the reduction of cell viability by TG was recovered when all three cell lines were treated with pan-caspase inhibitor. These results suggest that activation of apoptotic caspases by TG causes lipotoxic effect and decreases cell viability.

Hyposanthine이 포유동물 난자의 핵성숙에 미치는 영향 (Studies on the Effect of Hypoxanthine on Nuclear Maturation of Mammalian Oocytes)

  • 지희준
    • 한국가축번식학회지
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    • 제20권4호
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    • pp.427-432
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    • 1997
  • These studies were performed to approach the precise pathway inducing the meiotic inhibitory action of hypoxanthine on mouse follicular oocytes and to identify the cause of detrimental effect of hypoxanthine on viability of the oocyte in vitro. In addition, a correlation between the meiotic inhibitory effect and the detrimental effect of hypoxanthine was investigated. Mouse follicular oocytes at germinal vesicle(GV) stage were collected from the ovaries of ICR mice by puncturing the antral follicles with a fine needle, at 48 hours after PMSG injection. Oocytes were cultured in Modified Whittingham's T6 media containing hypoxanthine and several materials that involved in metabolism of hypoxanthine, and the effects of the materials on the actions of hypoxanthine were investigated by observing germinal vesicle breake down (GVBD), 1st polar body (PB) extrusion and viability of the oocytes. Phophodiesterase significantly reduced the meiotic inhibitory effect of dbcAMP but did not influence on the inhibitory effect of hypoxanthine. Allopurinol and 6-MP significantly enhanced the meiotic inhibitory effect of hypoxanthine, but the materials themselves also showed the meiotic inhibitory action like hypoxanthine. Hypoxanthine-guanine phosphoribosyltransferase significantly enhanced the meiotic inhibitory effect of hypoxanthine, on the contrary HGPRT itself promoted meiotic resumption of the oocytes. Catalase did not induce any change in the meiotic inhibitory effect of hypoxanthine, but SOD increased the GVBD rate suppressed by hypoxanthine. The detrimental effect of hypoxanthine on viability of the oocytes was significantly reduced by allopurinol and catalase, but SOD increased the GVBD rate suppressed by hypoxanthine. The detrimental effect of hypoxanthine on viability of the oocytes was significantly reduced by allopurinol and catalase, but SOD did not reduce the deterimental effect of hypoxanthine. In conclusion, the meiotic inhibtory effect of hypoxanthine may be caused by guanyl dervartives converted from hypoxanthine via salvage pathway, and superoxide anion may partially participate in the inhibitory effect of hypoxanthine. The detrimental effect of hypoxanthine on viability of the oocytes be cused by hydrogen peroxide produced during the metabolism of hypoxanthine.

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해초에 부착하는 부착생물 군집의 생태학적 특성 - II. 물리화학적 요인이 잘피 및 부착생물에 미치는 영향 (Ecological Characteristics of the Epiphytes on Seagrass - II. Effects of Physico-chemical Factors on Eelgrass (Zostera marina L.) and Epiphytes)

  • 정미희;윤석현
    • 환경생물
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    • 제30권3호
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    • pp.272-279
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    • 2012
  • 이 연구는 해초에 부착하는 부착생물 군집의 생태학적 특성에 대한 두 번째 연구로서 해초가 서식하는 연안 환경의 물리화학적 요인 변화에 따른 부착조류를 포함한 부착생물의 변화양상을 이해하고자 하는데 목적이 있다. 결과를 살펴보면, 1) 잘피는 수주의 온도와 정상관 관계를 보임으로써 잘피의 성장이 온도에 의해 큰 영향을 받고 있음을 알 수 있었으며, 2) 부착생물은 수온과는 상관관계를 보이지 않은 반면 수주의 염분과는 역상관 관계를 보였다. 이는 염분 25 이하 범위에서 서식이 용이한 미세규조 종들의 증가로 인한 것으로 보인다. 이는 영양염의 결과와도 일치하였는데, 3) 잘피의 성장과 총질산염 (TN)과는 역상관 관계를 보임으로써 수주에 총질산염이 낮을 때 잘피의 성장이 좋았으며, 4) 부착생물의 현존량과 인산염과는 유의성이 나타나지 않은 반면, 수주의 질산염, 아질산염 그리고 총질산염과는 정상관 관계를 나타냄으로써 부착생물의 성장에 질산염 계열의 영양염의 영향이 큰 것으로 나타났다. 영양염과 잘피의 성장, 그리고 부착생물의 현존량과의 상관관계 결과들을 종합해 보면 부착생물의 현존량 증가는 영양염의 증가로 인한 것이며, 이는 잘피의 성장을 감소시키는 원인-영향 과정과 깊은 관계가 있음을 나타내고 있다.

Atorvastatin inhibits the proliferation of MKN45-derived gastric cancer stem cells in a mevalonate pathway-independent manner

  • Choi, Ye Seul;Cho, Hee Jeong;Jung, Hye Jin
    • The Korean Journal of Physiology and Pharmacology
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    • 제26권5호
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    • pp.367-375
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    • 2022
  • Gastric cancer stem cells (GCSCs) are a major cause of radioresistance and chemoresistance in gastric cancer (GC). Therefore, targeting GCSCs is regarded as a powerful strategy for the effective treatment of GC. Atorvastatin is a widely prescribed cholesterol-lowering drug that inhibits 3-hydroxy-3-methylglutaryl-coenzyme A reductase, a rate-limiting enzyme in the mevalonate pathway. The anticancer activity of atorvastatin, a repurposed drug, is being investigated; however, its therapeutic effect and molecular mechanism of action against GCSCs remain unknown. In this study, we evaluated the anticancer effects of atorvastatin on MKN45-derived GCSCs. Atorvastatin significantly inhibited the proliferative and tumorsphere-forming abilities of MKN45 GCSCs in a mevalonate pathway-independent manner. Atorvastatin induced cell cycle arrest at the G0/G1 phase and promoted apoptosis by activating the caspase cascade. Furthermore, atorvastatin exerted an antiproliferative effect against MKN45 GCSCs by inhibiting the expression of cancer stemness markers, such as CD133, CD44, integrin α6, aldehyde dehydrogenase 1A1, Oct4, Sox2, and Nanog, through the downregulation of β-catenin, signal transducer and activator of transcription 3, and protein kinase B activities. Additionally, the combined treatment of atorvastatin and sorafenib, a multi-kinase targeted anticancer drug, synergistically suppressed not only the proliferation and tumorsphere formation of MKN45 GCSCs but also the in vivo tumor growth in a chick chorioallantoic membrane model implanted with MKN45 GCSCs. These findings suggest that atorvastatin can therapeutically eliminate GCSCs.

Binding Pattern Elucidation of NNK and NNAL Cigarette Smoke Carcinogens with NER Pathway Enzymes: an Onco-Informatics Study

  • Jamal, Qazi Mohammad Sajid;Dhasmana, Anupam;Lohani, Mohtashim;Firdaus, Sumbul;Ansari, Md Yousuf;Sahoo, Ganesh Chandra;Haque, Shafiul
    • Asian Pacific Journal of Cancer Prevention
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    • 제16권13호
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    • pp.5311-5317
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    • 2015
  • Cigarette smoke derivatives like NNK (4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone) and NNAL (4-(methylnitrosamino)-1-(3-pyridyl)-1-butan-1-ol) are well-known carcinogens. We analyzed the interaction of enzymes involved in the NER (nucleotide excision repair) pathway with ligands (NNK and NNAL). Binding was characterized for the enzymes sharing equivalent or better interaction as compared to +Ve control. The highest obtained docking energy between NNK and enzymes RAD23A, CCNH, CDK7, and CETN2 were -7.13 kcal/mol, -7.27 kcal/mol, -8.05 kcal/mol and -7.58 kcal/mol respectively. Similarly the highest obtained docking energy between NNAL and enzymes RAD23A, CCNH, CDK7, and CETN2 were -7.46 kcal/mol, -7.94 kcal/mol, -7.83 kcal/mol and -7.67 kcal/mol respectively. In order to find out the effect of NNK and NNAL on enzymes involved in the NER pathway applying protein-protein interaction and protein-complex (i.e. enzymes docked with NNK/NNAL) interaction analysis. It was found that carcinogens are well capable to reduce the normal functioning of genes like RAD23A (HR23A), CCNH, CDK7 and CETN2. In silico analysis indicated loss of functions of these genes and their corresponding enzymes, which possibly might be a cause for alteration of DNA repair pathways leading to damage buildup and finally contributing to cancer formation.