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http://dx.doi.org/10.5808/gi.21041

2-Methoxy-1,4-naphthoquinone (MNQ) regulates cancer key genes of MAPK, PI3K, and NF-κB pathways in Raji cells  

Wong, Teck Yew (Centre for Cancer Research, Universiti Tunku Abdul Rahman)
Menaga, Subramaniam (Centre for Cancer Research, Universiti Tunku Abdul Rahman)
Huang, Chi-Ying F. (Institute of Biopharmaceutical Sciences, National Yang-Ming University)
Ho, Siong Hock Anthony (School of Biosciences, Taylor's University, Lakeside Campus 1)
Gan, Seng Chiew (Department of Pre-Clinical Sciences Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman)
Lim, Yang Mooi (Centre for Cancer Research, Universiti Tunku Abdul Rahman)
Publication Information
Genomics & Informatics / v.20, no.1, 2022 , pp. 7.1-7.13 More about this Journal
Abstract
2-Methoxy-1,4-naphthoquinone (MNQ) has been shown to cause cytotoxic towards various cancer cell lines. This study is designed to investigate the regulatory effect of MNQ on the key cancer genes in mitogen-activated protein kinase, phosphoinositide 3-kinase, and nuclear factor κB signaling pathways. The expression levels of the genes were compared at different time point using polymerase chain reaction arrays and Ingenuity Pathway Analysis was performed to identify gene networks that are most significant to key cancer genes. A total of 43 differentially expressed genes were identified with 21 up-regulated and 22 down-regulated genes. Up-regulated genes were involved in apoptosis, cell cycle and act as tumor suppressor while down-regulated genes were involved in anti-apoptosis, angiogenesis, cell cycle and act as transcription factor as well as proto-oncogenes. MNQ exhibited multiple regulatory effects on the cancer key genes that targeting at cell proliferation, cell differentiation, cell transformation, apoptosis, reduce inflammatory responses, inhibits angiogenesis and metastasis.
Keywords
2-methoxy-1,4-naphthoquinone; cancer chemoprevention; MAPK pathway; nuclear factor-kappa B pathway; PI3K pathway;
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