• 한국수산과학회지
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• 제27권3호
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• pp.240-246
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• 1994

우렁쉥이 껍질 색소추출물을 이용한 무지개송어 체색 및 육색 침착효과를 검정하기 위하여 농도별, 기간별 급이실험을 하였다. 1. 체색의 개선효과는 사료급이 4주까지는 대조구와 비슷하였으나 6주후부터는 색소추출물 4(1,600ppm), 5구(3,200ppm) 및 pink구에서 효과가 나타났다. 명도(L)는 급이기간에 따라 감소하였으며 배측부위가 꼬리부위보다 약간 높았고, 적색도(a)는 8주후 꼬리부위가 배측부위보다 높아 색소추출물 4, 5구 및 pink구가 각각 4.7, 5.1, 5.9로 나타났다. 황색도(b)의 경우 배측부위는 감소하는 경향이었으나, 꼬리부위는 색소추출물 4, 5구 및 pink구는 증가하였지만 다른 구들은 변화가 적었다. 2. 육색의 개선효과는 체색의 개선효과보다 낮았으나 8주후에는 배측부위에서의 적색도(a)는 색소추출물 4, 5구 및 pink구가 각각 4.3, 4.6, 5.1로, 꼬리부위에서는 각각 4.7, 5.0, 5.4로 나타났다. 명도(L)는 색소추출물구가 증가한 반면 pink구는 거의 비슷하였다. 황색도(b)도 색소추출물구가 높아 전체적인 육색은 색소추출물구가 pink구보다 밝은 색을 띠는 것으로 나타났다. 3. 우렁쉥이 껍질 색소 추출물의 적정 투여량은 1,600ppm이상, 투여기간은 8주 이상이 되어야 할 것으로 나타났다.

• 한국환경보건학회지
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• 제33권4호
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• pp.306-316
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• 2007

This study have a object to found out the effects of oriental herb medicine, Antaeeum, to dams of rats and their offsprings. The Antaeeum was savaged to female Sprague-Dawley rats at a dose of 5 mg/kg/day for 3 weeks during gestation periods. Dams of rat were sacrificed at 20th day of gestation, and were observed major internal and reproductive organs. Approximately live fetuses in the 20th days of gestation were selected randomly and examined with stereo microscopes. Others offsprings were fixed with 95% ethanol for skeletal examinations. The fixed fetuses were stained with alcian blue and alizarin red S to observe skeletal variations or malformations. Maternal body weight of Antaeeum treated dams have a tendency of increasing compared with control dams. There were no significant difference in internal and reproductive organs of weight or findings. The spleenic organ relative weight of treated dams were decreased compared with the control significaltly (p<0.05). There were no significant changes between two groups in blood chemistry and hematological values. There were no significant changes in number of corpus luteum, implantation, live fetuses and implantation rate, delivery rate, late resorption rate and sex ratio. But in the Antaeeum treated group showed lower early resorption rate than that of the control dams. Fetal body weight and number of fetus a dam at Antaeeum treated group were higher than that of control group. The fetuses of dams treated with Antaeeum didn't induced external malformations. Vertebral and sternal variations were observed in Antaeeum group, but compared with the control, those variations were not significant. The ossification numbers of rib, cervical, thoracic, and lumber were normal. Fetuses treated with Antaeeum to the dams showed no significant difference in the number of caudal vertebra (P>0.01). From these results, it can be concluded that Antaeeum showed no toxicity effects on maternal side especially on body weight, early resorption rate, and number of live fetuses. Also there were no significant changes on maternal organ weights except spleen, hematological data, reproductive organs. Although skeletal variations were examined at vertebra and sternum, this Antaeeum could not induced significant choses in bone malformation.

• Journal of Korean Neurosurgical Society
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• 제46권5호
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• pp.468-471
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• 2009

Objective : The adolescent presentation of tethered cord syndrome (TCS) is well-recognized, but continues to pose significant diagnostic and management controversies. The authors conducted a retrospective study of clinical outcomes after surgical intervention in 24 school-aged children, adolescents, and young adults with TCS. Methods : All 83 patients with a lipomyelomeningocele (LMMC) underwent untethering surgery for caudal cord tethering between 1987 and 2007. The clinical charts and follow-up data were reviewed. Of these patients, 24 school-aged children, adolescents, and young adults with TCS were studied with respect to the clinical, radiologic, pathologic features, and surgical outcomes. Results : Untethering procedures were performed in 24 patients (age range, 7-25 years) for TCS of various origins (lipoma, lipomyelomeningocele, and tight filum terminale). Specific circumstances involving additional tugging of the already tight conus, and direct trauma to the back precipitated the onset of symptom in 50% of the patients. Diffuse and non-dermatomal leg pain, often referred to the anorectal region, was the most common presenting symptom. Progressive sensorimotor deficits in the lower extremities, as well as bladder and bowel dysfunction, were also common findings, but progressive foot and spinal deformities were noted less frequently. The most common tethered lesions were intradural lipomas, thickened filum and fibrous band adhesions into the placode sac. The surgical outcome was gratifying in relation to pain and motor weakness, but disappointing with respect to resolution of bowel and bladder dysfunction. Of the 24 patients with TCS, pre-operative deficits improved after surgery in 14 (58.3%). remained stable in 8 (33.4%). and worsened in 2 (8.3%). Conclusion : The pathologic lesions of tethered cord syndrome in school-aged children, adolescents, and young adults, are mostly intradural lipomas and tight filum. It is suggested that the degree of cord traction results in neurologic dysfunction in late life due to abnormal tension, aggravated by trauma or repeated tugging of the conus during exercise. Early diagnosis and adequate surgical release might be the keys to the successful outcome in school-aged children, adolescents, and young adults with TCS.

• Parasites, Hosts and Diseases
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• 제23권1호
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• pp.1-6
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• 1985

Five cases infected with Rhabditis sp. were detected in a survey to examine the stool specimens from rural primary school children. A large number of the larvae of Rhabditis sp. detected by the direct cellophane thick smear were cultured by the alter paper method. The eBamination was carried out in April through June, 1980 in Tangjeong-Myon, Ahsan.Gun, and in September 1983 in Sandong-Eup, Yeongwol-Gun. The results obtained in this study were as follows: 1. Prevalence: Out of 925 children, 5(0.54%) children were found to be positive. The number of the detected larvae was 110/0.1gm of feces in case 1, 35 in case 2, 130 in case 3, 86 in case 4 and 62 in case 5. 2. Larvae: The larvae were prepared by means of the direct fecal smear and measured by a micrometer equipped in the microscope. Twelve (12) through 15-day old larvae in culture were $197.1{\mu}{\textrm{m}}$ long in average, and the maximum size of the matured stage larvae was $884.0{\times}25.9{\mu}{\textrm{m}}$. However, the length variation was ranged as 173.0 to $884.0{\mu}{\textrm{m}}$. 3. Adults: The size of clubbed adult female was $1, 357{\mu}{\textrm{m}}(1, 176~1, 419)$ in length and $80{\mu}{\textrm{m}}(79~82)$ in width. Length of buccal cavity was $33{\mu}{\textrm{m}}$. A long cylindrical esophagus($273{\mu}{\textrm{m}}$) of the worms with a valved posterior cardiac bulb and with median bulbar swelling was morphologically indicated. Ditance from mouth to vulva was occupied 58% of body length. Male worm was $1, 006{\mu}{\textrm{m}}(890~1, 148)$ in length and $49{\mu}{\textrm{m}}(48~49)$ wide. Caudal alee of bursa and spicules ($75{\mu}{\textrm{m}}$ in length) were well developed. 4. Eggs: The oval shaped eggs in the female uterus, when cultured, were $66{\times}56{\mu}{\textrm{m}}$ in sixte, and the eggs laid by the adult could not be detected. So, reproduction might be thought to be ovoviviparity. 5. The five cases were re-examined during the period from the 1st to the 3rd week after stool examinations, but Rhabditis sp. were detected again in 4 cases in 1st week. When they were examined in 3rd week, larvae could not be detected. So, it was thought that the infection of Rhabditis sp. to humans was facultative.

• 한국발생생물학회지:발생과생식
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• 제15권2호
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• pp.121-131
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• 2011

Amphiprion melanopus의 난발생과 자치어의 형태학적 발달에 대한 기초 조사와 함께, 먹이생물과 갑상선호르몬이 자치어의 성장과 체색에 미치는 영향을 조사하였다. 수정 후, 부화까지 총 168시간이 소요되었다. 갓 부화 자어의 크기는 평균 $3.5{\pm}0.3$ mm 으며, 대부분의 자어는 부화 후 경과일 수에 따라 체색 변화(변태)를 나타내었다; DAH(days afterhatching) 10: 체색 흑화, DAH 15~20: 3개의 흰 줄무늬 출현, DAH 30: 등과 꼬리지느러미의 흰 줄무늬가 사라지기 시작,DAH 90: 체색 주황색; 성어: 체색은 진한 계피색이며 머리에만 흰 줄무늬 남음. Artemia와 T. japonicus를 함께 공급한실험구의 성장은 Artemia만 공급한 실험구보다 빨랐다. T. japonicus 만을 공급한 실험구의 지느러미 체색은 진하고 선명한 주황색이었다. 갑상선호르몬을 처리한 4 ppm구의 성장이 다른 실험구(0, 2, 6 ppm)보다 빨랐고 6 ppm구가 가장 느렸다. 본 연구의 결과, 주요 먹이생물로 알려져 있는 Artemia와 함께 T. japonicus를 공급함으로써 A. melanopus의 성장과체색을 개선시킬 수 있었고, 갑상선호르몬 또한 변태 및 체색 형성에 직접적으로 관여할 것으로 사료된다.

• 한국수정란이식학회지
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• 제31권4호
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• pp.335-347
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• 2016

Multiple interferon tau (IFNT) genes exist in bovine. An antiluteolytic substance secreted by the bovine conceptus and primarily responsible for maternal recognition of pregnancy is bovine trophoblast protein 1 (bIFNT1), a new type I interferon tau (IFNT) genes. The objectives of this research were to investigate whether multiple, distinct gene encode bIFNT1 and other type I bIFNT gene in the bovine genome and to examine expression of bIFNT1 and other bIFNTc1 mRNAs during conceptus development. These transcrips could be regulated through caudal-related homeobox-2 (CDX2) and ETS2 and/or AP1 (JUN) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. The presence of mRNAs encoded by bIFNT1 and type I bIFNTc1 genes were examined quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis of total cellular RNA (tcRNA) extracted from on day 17, 20 and 22 bovine conceptuses. The expression level of bIFNT1 was higher on day 17 transcripts were gradually weakly detectable on day 20 and 22. However, the other bIFNTc1 gene examined transcripts was highly expressed on day 20 and transcripts were weakly detectable on day 17 and 22 bovine conceptuses. Furthermore, human choriocarcinoma JEG3 was co-transfected with an -1kb-bIFNT1/c1-Luc constructs and several transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1-Luc increased when this constructs were co-transfected with, ETS2, AP1(JUN), CREBBP and/or CDX2. Also, bIFNTc1 gene was had very effect on activity by alone ETS2, and AP1 (JUN) expression factors in choriocarcinoma JEG3 cell. However, bIFNT1 gene expression of the upstream region was not identified. We demonstrated that the activities of bIFN genes are regulated by differential, tissue-specific and developmental competence during pregnancy.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.61-61
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• 2001

The main goal of this study was to produce transgenic piglets by the method of injection of sperm-mediated exogenous DNA. Spermatozoa (1$\times$106 sperm of final concentration) obtained from caudal epididymis were mixed with pBC1-hEPO (20 ng/${mu}ell$) or pcDNA3 LAC Z (20 ng/${mu}ell$), and followed by electroporation (500 V, 25 ㎌). Matured oocytes having the first polar body and dense cytoplasm were selected and centrifuged at 12,000g for 6 min. After sperm injection, the oocytes were activated electrically (1.7 ㎸/cm, 30 $\mu$ sec, single pulse) in 0.3 M mannitol solution. Eggs injected sperm were cultured in NCSU 23 medium (0.4% BSA) at 39$^{\circ}C$, 5% $CO_2$ in air for 192 h. This study were comprised 3 experiments. Experiment 1 compared the developmental efficiencies between the sperm-injected oocytes (Group 1) and further activated electrically (Group 2). Experiment 2 compared the expression of pcDNA3 LAC Z in the embryos produced by Group 1 and Group 2. Finally, experiment 3 carried out transfer of embryos (1-8 cell stage) transfected with pBC1 -hEPO into surrogate recipients synchronized by injection of combination of PG600 with hCG. The rates of cleavage and development into blastocyst stage in Group 2 were significantly higher than those of Group 1 (71.3% and 28.1% vs. 43.3% and 10.3%, respectively, p<0.05). Thirty (24.2%) out of 124 embryos analyzed in Group 2 were positive by X-gal. Similarly, in Group 1, 16.3% (8/49) were positive. After transfer of 789 embryos to 7 recipient gilts, three out of them examined by ultrasound became pregnant. One recipient is in day 50 pregnancy. On day 54 of gestation, two were carried out uterotomy in order to confirm the pregnancy One had 7 and another had 2 fetuses. We conclude that injection of sperm-mediated gene transfer will be used as a valuable tool for the production of transgenic piglets.

• 한국수산과학회지
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• 제22권3호
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• pp.129-137
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• 1989

1986년 12월 1일 강원도 명주군 옥계면 김진삼 이앞 연안에서 삼중책망으로 채포한 성숙한 도루묵을 이용하여 현장(선상)에서 인공수정시켜, 실내 사육실로 운반한 후 난발생과정과 부화자치어의 형태를 연구하였다. 난은 구형이고 침성점착난이며 난경은 $3.1\~3.4$mm(평균 3.3 mm, n=10)이었고, 많은 소유구를 가진다. 자연산난된 알은 지름이 약 4 cm인 알덩이를 형성하였다. 수온 $8.7\~12.3^{\circ}C$ 범위에서 수정 후 65일만에 부화되었다(적산수온 $663\~902^{\circ}C$). 부화직후 자어는 전장 $8.5\~10.2$ mm 범위이며, 근절수는 11+40(?)이었고, 가슴지느러미 줄기 5개가 발달하여 있었다. 부화 후 24일 째 전장 19.4 mm 자어는 난황을 완전히 흡수하였고, 배지느러미의 원기가 나타났다. 부화 후 32일째 전장 23.4 mm는 각 지느러미 줄기가 완성되고 치어기에 달한다. 부화 후 56일째 전장 29.9 mm의 치어는 체형이 거의 성어와 닮으며 체측에 4개의 흑색소포덩이가 나타났다. 전새개골 극은 전장 15.6 mm일때 4개가 나타났고 전장 24.4 mm에서 5개가 생겼다. 가슴지느러미는 전장 15 mm 전후에 둥근형에서 성어의 형태로 바뀌었다. 도루묵의 자어는 항문의 위치가 몸중앙보다 앞쪽에 위치한 점과 꼬리의 배쪽 가장자리를 따라 흑색소포가 발달한 점이 배도라치류의 자어와 닮았으나, 전새개골에 극을 가지고 있어 극이 없는 배도라치류의 자어와 구별되었다.

• 대한의용생체공학회:의공학회지
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• 제38권1호
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• pp.16-24
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• 2017

For the development of feasible retinal prosthesis, one of the important elements is acquiring proper judging tool if electrical stimulus leads to patient's visual perception. If evoked potential to electrical stimulus is recorded in primary visual (V1) cortex, it means that the stimulus effectively evokes visual perception. Therefore, in this study, we established VEP recording system on V1 cortex using BioPAC modules as the judging tool. And the measuring system was evaluated by recording VEP of mice. After anesthesia, normal mice (C57BL/6J strain; n = 6) were secured to stereotaxic apparatus (Harvard Apparatus, USA). For the recording of VEP, the stainless steel needle electrode (impedance: $2-5k{\Omega}$) was positioned on the surface of the cortex through the burr hole at 2.5 mm lateral and 4.6 mm caudal to bregma. DA 100C and EEG 100C BioPAC modules were used for the trigger signal and VEP recording, respectively. When left eye was blocked by black cover and right eye was stimulated by flash light using HMsERG (RetVet Corp, USA), VEP response at left V1 cortex was detected, but there was no response at right V1 cortex. Amplitudes and latencies of P2, N3 peaks of VEP recording varied according to the depths of the electrodes on V1 cortex. From the surface upto $600{\mu}m$ depth, amplitudes of P2 and N3 increased, while deeper than $600{\mu}m$, those amplitudes decreased. The deeper the insertion depth of the electrode, the latency of N1 peaks tends to be delayed. However, there was no statistically significant difference among the latencies of P2 and N3 peaks (P > 0.05, ANOVA). Our VEP recording data such as the insertion depth and the latency and amplitudes of peaks might be used as guidelines for electrically-evoked potential (EEP) recording experiment in near future.

• 한국수산과학회지
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• 제31권3호
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• pp.309-316
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• 1998

참복과 (Tetraodontidae) 어류의 외부형태 형질 가운데 후각기관, 옆줄, 피부극. 체형 및 체색의 특징을 조사하기 위하여. 1989년 9월부터 1994년 7월까지 우리나라 20개 지점에서 10속 27종의 표본을 채집하여 관찰한 결과는 다음과 같다. 1. 참복과 어류 가운데 참복속(Takifugu), 밀복속(Lagocephalus), 은띠복속(Plurancanthus). 불룩복속(Spheoides) 및 대치복속 (Feroxodon) 어류의 콧구멍은 2개이며. 수지복속 (Ephippion)과 청복속 (Canthigaster) 어류는 1개이고, 반면에 별복속 (Boesemanichthys), 꺼끌복속(Arothron) 및 첼로복속 (Chelonodon) 어류는 없다. 2. 밀복속, 불룩복속, 별복속, 꺼끌복속 및 대치복속어류의 옆줄은 몸의 옆쪽에 단지 1열로 잘 발달되어 있으며, 참복속과 은띠복속 어류는 2열이고, 첼로복속과 수지복속 어류는 꼬리자루 부분에서 2열로 융합되어 있다. 3. 청복속 어류는 옆줄 및 후각기관의 형태가 참복과의 다른 속 어류와 많은 차이를 보여 구별되었다 4. 수지복(Ephippion guttifer)의 피부극은 비늘이 완전하게 변형되어 등쪽과 옆쪽의 것은 딱딱한 나무껍질 모양을 하며, 꼬리쪽의 것은 긴 막대기 모양으로 특이하였다. 5. 피부극을 가지고 있는 종은 22종으로 밀복속 어류와 참복속의 까칠복 (Takifugu stictonotus) 및 꺼끌복속(Arothron)의 꺼끌복 (A. Stellatus)의 피부극은 등쪽의 것보다 배쪽의 것이 크고, 첼로복 (Chelonodon patoca)과 청복 (Canthigaster tivulata)은 등쪽의 피부극의 뿌리가 3개이었다. 6. 밀복속 어류의 대부분은 피부극을 등쪽과 배쪽에 가지고 있으나, 민밀복 (L. inermis)은 몸 전체에 피부극이 없는 점에서 밀복 (L. lunaris), 은밀복 (L. wheeleri), 흑밀복 (L.gloveri) 및 은민밀복 (L. laevigatus)과 구별된다.