• Title/Summary/Keyword: case markers

검색결과 277건 처리시간 0.035초

Hepatitis C Virus Prevalence and Genotyping among Hepatocellular Carcinoma Patients in Baghdad

  • Al-Kubaisy, Waqar Abd Al Qahar;Obaid, Kadhim Jawad;Noor, Nor Aini Mohd;Ibrahim, Nik Shamsidah Binti Nik;Al-Azawi, Ahmed Albu-Kareem
    • Asian Pacific Journal of Cancer Prevention
    • /
    • 제15권18호
    • /
    • pp.7725-7730
    • /
    • 2014
  • Hepatocellular carcinoma (HCC) is the third most common cause for cancer death in the world, now being especially linked to chronic hepatitis C virus (HCV) infection. This case-control study consisting of 65 HCC patients and 82 patients with other malignant tumours as controls was conducted to determine the association of HCV markers with HCC. Serum of each participant was obtained for detection of HCV Ab and RNA by DNA enzyme immunoassay (DEIA). Twenty six per cent (26.0%) of HCC patients had positive anti-HCV which was significantly greater than the control group (p=0.001). HCC patients significantly have a risk of exposure to HCV infection almost 3 times than the control group (OR=2.87, 95% C.I=1.1-7). Anti-HCV seropositive rate was significantly (p=0.03) higher among old age HCC patients and increases with age. Males with HCC significantly showed to have more than 9 times risk of exposure to HCV infection (OR=9.375, 95 % CI=1.299-67.647) than females. HCV-RNA seropositive rate was (70.8%) significantly higher among HCC patients compared to (22.2%) the control group (p=0.019). The most prevalent genotype (as a single or mixed pattern of infection) was HCV-1b. This study detected a significantly higher HCV seropositive rate of antibodies and RNA in HCC patients.

Evaluation of a New Fine-mapping Method Exploiting Linkage Disequilibrium: a Case Study Analysing a QTL with Major Effect on Milk Composition on Bovine Chromosome 14

  • Kim, JongJoo;Georges, Michel
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제15권9호
    • /
    • pp.1250-1256
    • /
    • 2002
  • A novel fine-mapping method exploiting linkage disequilibrium (LD) was applied to better refine the quantitative trait loci (QTL) positions for milk production traits on bovine chromosome 14 in the pedigree comprising 22 paternal half-sib families of a Black-and-White Holstein-Friesian grand-daughter design in the Netherlands for a total of 1,034 sons. The chromosome map was constructed with the 31 genetic markers spanning 90 Kosambi cM with the average inter-marker distance of 3.5 cM. The linkage analyses, in which the effects of sire QTL alleles were assumed random and the random factor of the QTL allelic effects was incorporated into the Animal Model, found the QTL for milk, fat, and protein yield and fat and protein % with the Lod scores of 10.9, 2.3, 6.0, 25.4 and 3.2, respectively. The joint analyses including LD information by use of multi-marker haplotypes highly increased the evidence of the QTL (Lod scores were 25.1, 20.9, 11.0, 85.7 and 17.4 for the corresponding traits, respectively). The joint analyses including DGAT markers in the defined haplotypes again increased the QTL evidence and the most likely QTL positions for the five traits coincided with the position of the DGAT gene, supporting the hypothesis of the direct causal involvement of the DGAT gene. This study strongly indicates that the exploitation of LD information will allow additional gains of power and precision in finding and localising QTL of interest in livestock species, on the condition of high marker density around the QTL region.

Current Research Status for Economically Important Candidate Genes and Microarray Studies in Cattle (소의 경제형질 관련 후보 유전자 및 Microarray 연구현황)

  • 유성란;이준헌
    • Journal of Animal Science and Technology
    • /
    • 제48권2호
    • /
    • pp.169-190
    • /
    • 2006
  • Researches in livestock are currently actively progressing to improve economically important traits using DNA markers. In cattle, the candidate genes have been selected based on their known functions in the target QTL (quantitative trait locus) region in order to identify QTN (quantitative trait nucleotide) for improving productivities. In this review, molecular genetic studies for the meat related traits, one of the major determinant of market prices, have been fully described. Also recent emerging microarray technique for identifying candidate genes in cattle has been discussed. In case of microarray, cDNA microarrays have been replaced to oligoarrays in order to minimize the experimental errors in cattle. Since the first draft of bovine genome sequences was appeared in the public domain, more markers in relation to the quantitative traits will be discovered in a short period of time and genes affecting difficult-to-measure traits, such as disease resistance, can also be selected for marker assisted selection in near future.

Development of Facial Palsy Grading System with Three Dimensional Image Processing (3차원 영상처리를 이용한 안면마비 평가시스템 개발)

  • Jang, M.;Shin, S.H.
    • Journal of rehabilitation welfare engineering & assistive technology
    • /
    • 제9권2호
    • /
    • pp.129-135
    • /
    • 2015
  • The objective grading system for the facial palsy is needed. In this study, the facial palsy grading system was developed with combination of three dimensional image processing and Nottingham scale. The developed system is composed of 4 parts; measurement part, image processing part, computational part, facial palsy evaluation & display part. Two web cam were used to get images. The 8 marker on face were recognized at image processing part. The absolute three dimensional positions of markers were calculated at computational part. Finally, Nottingham scale was calculated and displayed at facial palsy evaluation & display part. The effects of measurement method and position of subject on Nottingham scale were tested. The markers were measured with 2-dimension and 3-dimension. The subject was look at the camera with 0 and 11 rotation. The change of Scale was large in the case of 11 rotation with 2-dimension measurement. So, the developed system with 3-dimension measurement is robust to the orientation change of subject. The developed system showed the robustness of grading error originated from subject posture.

  • PDF

Development of a Tensile Cell Stimulator to Study the Effects of Uniaxial Tensile Stress on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells (세포 인장 자극기의 개발과 세포 인장 자극을 통한 성체 줄기세포의 골분화 유도)

  • Shin, Hyun-Jun;Lee, Woo-Teak;Park, Suk-Hoon;Lee, Sun-Hwa;Park, Jung-Ho;Yoon, Yong-San;Shin, Jennifer H.
    • Transactions of the Korean Society of Mechanical Engineers A
    • /
    • 제33권7호
    • /
    • pp.629-636
    • /
    • 2009
  • Mechanical stimulation is known to play a vital role on the differentiation of mesenchymal stem cells (MSCs) to pre-osteoblasts. In this research, we developed a tensile cell stimulator, composed of a DC motor-driven actuator and LVDT sensor for measuring linear displacement, to study the effects of tensile stress on osteogenic differentiation of MSCs. First, we demonstrated the reliability of this device by showing the uniform strain field in the silicon substrate. Secondly, we investigated the effects of tensile stretching on osteogenic differentiation. We imposed a pre-set cyclic strain at a fixed frequency on cell monolayer cultured on a flexible silicon substrate while varying its amplitude and duration. 60 min of resting period was allowed between 30 min of cyclic stretching and this cycle is repeated up to 7 days. Under the combined stimulation with osteogenic media and mechanical stretching, the osteogenic markers such as alkaline phosphatase (ALP), osterix, and osteopontin began to get expressed as early as 4 days of stimulation, which is much shorter than what is typically required for osteogenic media induced differentiation. Moreover, different markers were induced at different magnitudes of the applied strains. Lastly, for the case of ALP, we observed the antagonistic effects of osteogenic media when combined with mechanical stretching.

Method Discrimination for Product Traceability and Identification of Korean Native Chicken using Microsatellite DNA (초위성체를 이용한 한국 재래닭의 원산지 추적 및 개체 식별 방법에 관한 연구)

  • Park, Mi-Hyun;Oh, Jae-Don;Jeon, Gwang-Joo;Kong, Hong-Sik;Sang, Byong-Don;Choi, Chull-Hwan;Yeon, Sung-Hum;Cho, Byong-Wok;Lee, Hak-Kyu
    • Korean Journal of Organic Agriculture
    • /
    • 제12권4호
    • /
    • pp.451-461
    • /
    • 2004
  • In an animals, identification system has been widely used by ear tag with dummy code and blood typing for parernity. Also, genotyping methods were using for useful mean of individual identification for live animals. In the case of genotyping estimation of gene in population of korean native chicken. In this study, we tested for development of genetic markers used it possible to determination of individual identification system. The candidate genetic markers were used already bow 10 of microstalite DNA sequence information in chromosome No. 1 and 14. Result of analysis for genotyping, the number of alleles of those microstatelites DNA was shown minimal 3 to 12 and the heterozygote expression frequency range was shown from 0.617 to 0.862. In our result, effective number of allele for each microsatellites DNA was shown 3~7, and the accuracy of individual identification was shown nearly 100%, when used with 6 genetic marker. This study was about genotyping method for identification used specific genetic marker form microsatellite DNA in the brand marketing of korean native chicken. Our results suggest that genotyping method used specific genetic marker from microsatellite DNA might be very useful for determination of individual identification.

  • PDF

Passive 3D motion optical data in shaking table tests of a SRG-reinforced masonry wall

  • De Canio, Gerardo;de Felice, Gianmarco;De Santis, Stefano;Giocoli, Alessandro;Mongelli, Marialuisa;Paolacci, Fabrizio;Roselli, Ivan
    • Earthquakes and Structures
    • /
    • 제10권1호
    • /
    • pp.53-71
    • /
    • 2016
  • Unconventional computer vision and image processing techniques offer significant advantages for experimental applications to shaking table testing, as they allow the overcoming of most typical problems of traditional sensors, such as encumbrance, limitations in the number of devices, range restrictions and risk of damage of the instruments in case of specimen failure. In this study, a 3D motion optical system was applied to analyze shake table tests carried out, up to failure, on a natural-scale masonry structure retrofitted with steel reinforced grout (SRG). The system makes use of wireless passive spherical retro-reflecting markers positioned on several points of the specimen, whose spatial displacements are recorded by near-infrared digital cameras. Analyses in the time domain allowed the monitoring of the deformations of the wall and of crack development through a displacement data processing (DDP) procedure implemented ad hoc. Fundamental frequencies and modal shapes were calculated in the frequency domain through an integrated methodology of experimental/operational modal analysis (EMA/OMA) techniques with 3D finite element analysis (FEA). Meaningful information on the structural response (e.g., displacements, damage development, and dynamic properties) were obtained, profitably integrating the results from conventional measurements. Furthermore, the comparison between 3D motion system and traditional instruments (i.e., displacement transducers and accelerometers) permitted a mutual validation of both experimental data and measurement methods.

Mechanistic insight into the progressive retinal atrophy disease in dogs via pathway-based genome-wide association analysis

  • Sheet, Sunirmal;Krishnamoorthy, Srikanth;Park, Woncheoul;Lim, Dajeong;Park, Jong-Eun;Ko, Minjeong;Choi, Bong-Hwan
    • Journal of Animal Science and Technology
    • /
    • 제62권6호
    • /
    • pp.765-776
    • /
    • 2020
  • The retinal degenerative disease, progressive retinal atrophy (PRA) is a major reason of vision impairment in canine population. Canine PRA signifies an inherently dissimilar category of retinal dystrophies which has solid resemblances to human retinis pigmentosa. Even though much is known about the biology of PRA, the knowledge about the intricate connection among genetic loci, genes and pathways associated to this disease in dogs are still remain unknown. Therefore, we have performed a genome wide association study (GWAS) to identify susceptibility single nucleotide polymorphisms (SNPs) of PRA. The GWAS was performed using a case-control based association analysis method on PRA dataset of 129 dogs and 135,553 markers. Further, the gene-set and pathway analysis were conducted in this study. A total of 1,114 markers associations with PRA trait at p < 0.01 were extracted and mapped to 640 unique genes, and then selected significant (p < 0.05) enriched 35 gene ontology (GO) terms and 5 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways contain these genes. In particular, apoptosis process, homophilic cell adhesion, calcium ion binding, and endoplasmic reticulum GO terms as well as pathways related to focal adhesion, cyclic guanosine monophosphate)-protein kinase G signaling, and axon guidance were more likely associated to the PRA disease in dogs. These data could provide new insight for further research on identification of potential genes and causative pathways for PRA in dogs.

Improving hydration in elite male footballers during a national team training camp - an observational case study

  • Mohr, Magni;Nolsoe, Eli Leifsson;Krustrup, Peter;Fatouros, Ioannis G.;Jamurtas, Athanasios Z.
    • Korean Journal of Exercise Nutrition
    • /
    • 제25권4호
    • /
    • pp.10-16
    • /
    • 2021
  • [Purpose] The purpose of this study was to (i) assess hydration levels in elite male football players during a national team training camp before and during qualifying matches, (ii) evaluate the effect of coaching strategies for hydration based on feedback from hydration monitoring, and (iii) assess possible relationships between hydration status and training load or wellness markers. [Methods] Thirty-one male players (age 27±4 yrs; height 185±6 cm; weight 82.9±6.7 kg; body fat 10.4±2.3%) representing a national team from the Union of European Football Associations (UEFA) participated. The players were studied during three different national team training camps related to the UEFA Nations League tournament. Urine specific gravity (USG) was measured to assess hydration status. During all camps, the players were actively coached on improving strategies for hydration and given individual feedback on their test results. The training load was measured using GPS technology, and wellness questionnaires were completed. [Results] USG decreased progressively and significantly (p<0.005) during camp 1 and hydration status improved over the three camps, with fewer dehydrated and more well-hydrated players identified during the last part of camp 3. Significantly (p<0.05) higher USG values were observed 2 days prior to a match (MD-2) than on match day (MD); consequently, 52% of the players were dehydrated on MD-2 and only 6% on MD. No correlations were observed between hydration status and training load or wellness markers. [Conclusion] Dehydration is a challenge in elite male football, but continuous monitoring of hydration status and coaching on hydration strategies can lead to major improvements and reduce the degree of dehydration.

A Pediatric Case of Toxic Hepatitis Induced by Hovenia Dulcis

  • Kim, Yun Ji;Ryu, Seung Lok;Shim, Jae Won;Kim, Duk Soo;Shim, Jung Yeon;Park, Moon Soo;Jung, Hye Lim
    • Pediatric Gastroenterology, Hepatology & Nutrition
    • /
    • 제15권2호
    • /
    • pp.111-116
    • /
    • 2012
  • Toxic hepatitis is a rare but devastating disease in children. Herbs are widely used in oriental medicine to treat various symptoms in Korea, however, several herbs have been reported to induce liver injury. We report a case of toxic hepatitis induced by Hovenia dulcis in a 3-year-old boy. He complained of nausea, abdominal discomfort, and jaundice. The patient had consumed water boiled with hovenia dulcis for about 1 year prior to presentation. A diagnosis of toxic hepatitis was made based on his history, laboratory data, viral markers, ultrasonography, and biopsied liver tissue. We administered supportive management for acute fulminant hepatitis but his symptoms and liver function progressed. He was transferred to another hospital for further evaluation and consideration for liver transplantation. Because acute liver failure due to herbs or dietary supplement taken for a long time is often fetal, it is important to make early diagnosis and stop taking the drug as soon as drug induced liver injury is suspected.