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http://dx.doi.org/10.3795/KSME-A.2009.33.7.629

Development of a Tensile Cell Stimulator to Study the Effects of Uniaxial Tensile Stress on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells  

Shin, Hyun-Jun (한국과학기술원 기계공학과)
Lee, Woo-Teak (한국과학기술원 기계공학과)
Park, Suk-Hoon (한국과학기술원 기계공학과)
Lee, Sun-Hwa (고려대학교 의과대학 정형외과)
Park, Jung-Ho (고려대학교 의과대학 정형외과)
Yoon, Yong-San (한국과학기술원 기계공학과)
Shin, Jennifer H. (한국과학기술원 기계공학과)
Publication Information
Transactions of the Korean Society of Mechanical Engineers A / v.33, no.7, 2009 , pp. 629-636 More about this Journal
Abstract
Mechanical stimulation is known to play a vital role on the differentiation of mesenchymal stem cells (MSCs) to pre-osteoblasts. In this research, we developed a tensile cell stimulator, composed of a DC motor-driven actuator and LVDT sensor for measuring linear displacement, to study the effects of tensile stress on osteogenic differentiation of MSCs. First, we demonstrated the reliability of this device by showing the uniform strain field in the silicon substrate. Secondly, we investigated the effects of tensile stretching on osteogenic differentiation. We imposed a pre-set cyclic strain at a fixed frequency on cell monolayer cultured on a flexible silicon substrate while varying its amplitude and duration. 60 min of resting period was allowed between 30 min of cyclic stretching and this cycle is repeated up to 7 days. Under the combined stimulation with osteogenic media and mechanical stretching, the osteogenic markers such as alkaline phosphatase (ALP), osterix, and osteopontin began to get expressed as early as 4 days of stimulation, which is much shorter than what is typically required for osteogenic media induced differentiation. Moreover, different markers were induced at different magnitudes of the applied strains. Lastly, for the case of ALP, we observed the antagonistic effects of osteogenic media when combined with mechanical stretching.
Keywords
Osteogenic Differentiation; Mesenchymal Stem Cell; Strain Ratio; Cell Tensile Stimulation; 2D Elastic Membrane; Quantitative Analysis; Osteogenic Marker;
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