• Title/Summary/Keyword: carbon tetrachloride

Search Result 539, Processing Time 0.023 seconds

EFFECTS OF DICHLOROMETHANE ON CARBON TETRACHLORIDE HEPATOTOXICITY IN RATS

  • Kim, Dae B.;Kim, Baik H.
    • Toxicological Research
    • /
    • v.5 no.1
    • /
    • pp.37-41
    • /
    • 1989
  • A non-hepatotoxic dose of dichloromethane (DCM) was examined for potential effects on the hepatotoxicity of carbon tetrachloride (CT) in adult male rats. A concomitant treatment of DCM (0.45ml/kg, po) significantly potentiated the hepatotoxicity of CT at varing doses (0.06 to 0.63 ml/kg, po) as determined by increase in SGOT and SGPT activities 24 hn following the treatments. The carboxyhemoglobin (COHb) saturation induced by DCM was significantly decreased by CT treatments. The potentiation of CT hepatotoxicity by DCM does not appear to be associated with increased metabolism of CT.

  • PDF

Hepatoprotective Effect of Vitex negundo against Carbon Tetrachloride-Induced Liver Damage

  • Avadhoot, Y.;Rana, A.C.
    • Archives of Pharmacal Research
    • /
    • v.14 no.1
    • /
    • pp.96-98
    • /
    • 1991
  • Alcoholic extract of the seeds of Vitex negundo Linn. was obtained by cold maceration. A dose of 250 mg/kg $(1/6\;of\;LD_{50})$ of the extract was selected to study the hepatoprotective action against carbon tetrachloride-induced liver damage. The extract was found to be effective in preventing liver damage which was evident by morphological, biochemical and functional parameters.

  • PDF

Protective Effects of Extracts of Mori Cortex Radicis on Carbon Tetrachloride-induced Hepatotoxicity (사염화탄소에 의해 유발된 간독성에 대한 상백피 추출물의 간보호효과)

  • 김선여;이희삼;류강선;이은주;김영중
    • YAKHAK HOEJI
    • /
    • v.43 no.3
    • /
    • pp.391-396
    • /
    • 1999
  • In order to evaluate the protective effects of extracts of Mori cortex radicis on carbon tetrachloride-induced hepatotoxicity, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, lactic dehydrogenase, malondiadehyde values and glutathione S-transferase activity were measured in ICR mice. The activities of serum aminotransferase and the hepatic content of lipid peroxide after carbon tetrachloride-treatment were markedly increased than normal control but those levels were significantly decreased by the treatment of butanol fraction of Mori cortex radicis mathanol extract. Glutathione S-transferase activity was decreased by carbon tetrachloride than control, but that also inhibited by the treatment of butanol fraction of Mori cortex radicis methanol extract. These results demonstrate a possible hepatoprotective role of extract role of extract of Mori cortex radicis against ${CCl_4}-induced$ hepatoxicity in vivo.

  • PDF

Effects of Onion Diet on Carbon Tetrachloride Toxicity of Rats (양파식이가 흰쥐에서 사염화탄소 독성에 미치는 영향)

  • 이명렬;이병래;박평심
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.20 no.2
    • /
    • pp.121-125
    • /
    • 1991
  • This study designs to investigate effects of onion diet on carbon tetrachloride toxicity of rats. Experiments were performed with week's feeding, body weight, food intake, ratio of orgen weight/dody weight, serum lipid levels, superoxide dismutase and catalase activity and malondialdehyde(MDA) content in liver and kidney were determined. The content of serum total cholesterol in each group were lower than those of control group(p<0.05), especially at onion juice treated group. Serum HDL-cholesterol level of CBB and CBJ groups was significantly lower than that of control group (p<0.05). Superoxide dismutase activities of liver and kidney were significantly increased by carbon tetrachloride treatment and decreased by onion feeding. MDA contents in liver and kidney of CCl4 treated rats were significantly decreased by boiled and fresh onion fed group, compared with CCl4 treated control group. This result suggested that onion diet has a protective effect of CCl4 induced hepatotoxicity and nephrotoxicity of rat.

  • PDF

Hepatoprotective Effects of the Acteoside on Carbon tetrachloride Induced Liver damage in Mice

  • Lee, Kyung-Jin;Woo, Eun-Rhan;Choi, Chul-Yung;Jeong, Hye-Gwang
    • Proceedings of the PSK Conference
    • /
    • 2003.04a
    • /
    • pp.174.1-174.1
    • /
    • 2003
  • The protective effects of acteoside, a phenylethanoid glycoside, on cabon tetrachloride-induced hepatotoxicity and the possible mechanisms involved in this protection were investigated in mice. Pretreatment with acteoside prior to the administration of carbon tetrachloride significantly prevented the increased serum enzymatic activities of alanine and aspartate aminotransferase in a dose-dependent manner. (omitted)

  • PDF

Hepatoprotective Activity of Scopoletin, a Constituent of Solanum lyratum

  • Kang, So-Young;Sung, Sang-Hyun;Park, Jong-Hee;Kim, Young-Choong
    • Archives of Pharmacal Research
    • /
    • v.21 no.6
    • /
    • pp.718-722
    • /
    • 1998
  • Scopoletin (7-hydroxy-6-methoxycoumarin), a coumarin, was isolated from the aerial part of Solanum lyratum Thunb. by the activity-guided fractionation employing carbon te trachloride-intoxicated primary cultured rat hepatocytes as a screening system. Its hepatoprotective activity was first evaluated by measuring the release of glutamic pyruvic transaminase and sorbitol dehydrogenase from carbon tetrachloride-intoxicated rat hepatocytes into the culture medium. Scopoletin significantly reduced the releases of glutamic pyruvic transaminase and sorbitol dehydrogenase from the carbon tetrachloride-intoxicated primary cultured rat hepatocytes by 53% and 58%, respectively, from the toxicity in a dose-dependent manner over concentration ranges of 1mcM to 50mcM. Further studies revealed that at the concentration of 10mcM, scopoletin significantly preserved glutathione content by 50% and the activity of superoxide dismutase by 36% and also inhibited the production of malondialdehyde to the degree as seen in the control.

  • PDF

Protective Effects of Alnus japonica Steude on Hepatic Injury Induced by Carbon Tetrachloride in Rats (사염화탄소로 유발된 흰쥐의 간손상에 대한 오리나무 분획물의 간 보호효과)

  • Kim, Ok-Kyung
    • Korean Journal of Pharmacognosy
    • /
    • v.34 no.2 s.133
    • /
    • pp.172-178
    • /
    • 2003
  • This study was done to investigate the protective effect of Alnus japonica Steude on hepatotoxicity in carbon let-rachloride $(CCl_4)$ intoxicated rats. Alnus japonica Steud was extracted with methanol and fractionated with hexane, chloroform, ethylacetate, butanol and water. Rats were treated with those orally once a day for 6 days. The activities of aminotransferase and ${\gamma}-glutamyltranspeptidase$ and contents of cholesterol, TG and hepatic lipid peroxide in butanol fraction pretreated rats were significantly decreased compared to the only $CCl_4$ treated rats but the content of glutathione was significantly increased compared to the only $CCl_4$ treated rats. Also activities of hepatic superoside dismutase, catalase, glutathione perpxidase in butanol fraction pretreated rats were signigicantly decreased compared to the only $CCl_4$ treated rats. These result indicated that butanol fraction of Alnus japonica Steude showed hepatoprotective effect in carbon tetrachloride intoxicated rats.

Screening of Medicinal Plants Having Hepatoprotective Activity Effects with Primary Cultured Hepatocytes Intoxicated Using Carbon tetrachloride Cytotoxicity ($CCl_4$로 독성유발시킨 초대배양 간세포를 이용하여 간세포 보호효과를 나타내는 생약류의 검색)

  • Lee, June-Woo;Choi, Joon-Han;Kang, Sang-Mo
    • Korean Journal of Pharmacognosy
    • /
    • v.23 no.4
    • /
    • pp.268-275
    • /
    • 1992
  • We studied to screen medicinal plants having hepatoprotective activity with the primary cultured rat hepatocytes intoxicated with carbon tetrachloride cytotoxicity. The lowest concentration and treatment time of carbon tetrachloride giving the greatest intoxication to the primary cultured hepatocytes were observed in 10mM and 60 minutes, respectively. GTP and GOT activity of culture broth of the primary cultured rat hepatocytes intoxicated by $CCl_4$ cytotoxicity at this condition were increased 135.9% and 178.3% compared with that of the primaries cultured hepatocytes not treated with $CCl_4$, respectively. This increased GPT activity was inhibited by glycyrrizin, which was known to have hepatoprotective activity, and the inhibition activity was dependent on the concentration of glycyrrhizin. Forty species among the extracts obtained from 117 species of medicinal plants were shown to have the hepatoprotective activity. Among these 40 species, Prunus persica, Scutellaria baicalensis, Astragalus membranaceus, Tribulus terrestris, Caragana chamlagu, Acanthopanax sessiliflorum and Achyranthes japonica were indicated a lower GPT activity than that of Glycyrrhiza uralensis containing glycyrrhizin and GPT activity of these were indicated 75.5%, 70.0%, 59.0%, 77.5%, 60.0%, 75.0% and 79.0%, respectively.

  • PDF

Hepatoprotective Effects of Water Extract of Liriopis Tuber on Carbon Tetrachloride-Induced Hepatotoxicity in Rats (맥문동 열수추출물의 사염화탄소로 유발된 흰쥐의 간 손상에 대한 보호효과)

  • An, Ji-Yoon;Rhee, In-Ja
    • Korean Journal of Pharmacognosy
    • /
    • v.34 no.2 s.133
    • /
    • pp.166-171
    • /
    • 2003
  • The hepatoprotective effects of Liriopis tuber on carbon tetrachloride $(CCl_4)$ intoxicated rats were studied. The water extract prepared from Liriopis tuber were administrated to the pretreatment group orally once a day for successive 7 days, followed by treatment with $CCl_4$ on the seventh day. The post treatment group was treated with $CCl_4$ and then the water extract was administrated. The activities of glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), lactate dehydrogenase (LDH), and contents of total cholesterol and triglyceride in pretreatment rats serum were significantly decreased compared to the only $CCl_4$ treated rats. The level of high density lipoprotein (HDL) cholesterol was considerably increased compared to the only $CCl_4$ treated rats. In the pretreatment group with the water extract of Liriopis tuber tissue destruction was not observed in light microscopic investigation, compared to the only $CCl_4$ treated rats. These results suggest that Liriopis tuber have potent hepatoprotective effect against carbon tetrachloride intoxicated rats.

Protective Effects of Houttuynia cordata Thunb on Carbon Tetrachloride-induced Hepatotoxicity in Rats (어성초 분획물이 사염화탄소로 유발된 흰쥐의 간손상에 대한 보호효과)

  • Kim, Ok-Kyung
    • Korean Journal of Pharmacognosy
    • /
    • v.33 no.4 s.131
    • /
    • pp.324-331
    • /
    • 2002
  • This study was performed to investigate the protective effect of Houttuynia cordata Thunb on hepatotoxicity in carbon tetrachloride$(CCI_4)$ intoxicated rats. The examined effects hexane, chloroform, butanol and water fractions prepared from the Houttuynia cordata Thunb methanol exlract and rats were administrated with those orally once a day for successive 6 days, fellowed by treaoent with $CCl_4$ on the sixth day. After 6 days, the activities of aminotransferase, alkalinephosphatase, ${\gamma}-glutamyl$ transpeptidase, lactate dehydrogenase and contents of triglyceride, hepatic lipid peroxide in butanol fraction pretreated rats were significantly decreased compared to the only $CCl_4$ treated rats, also depletion glutathione content induced by treatment with $CCl_4$ was prevented by butanol fraction pretreated rats. In addition, activities of hepatic superoxide dismutase, catalase, glutathione peroxidase in butanol fiaction pretreated rats were significantly decreased compared to the only $CCl_4$ treated rats, but the activity of hepatic glutathione-S-transferase was not significantly effect. These results suggest that butanol fiuction of Houttuynia cordata Thunb methanol extract have potent hepatoprotective effect against carbon tetrachloride intoxicated rats.