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Protective Effects of Alnus japonica Steude on Hepatic Injury Induced by Carbon Tetrachloride in Rats  

Kim, Ok-Kyung (Department of Food and Nutrition, Daejin University)
Publication Information
Korean Journal of Pharmacognosy / v.34, no.2, 2003 , pp. 172-178 More about this Journal
Abstract
This study was done to investigate the protective effect of Alnus japonica Steude on hepatotoxicity in carbon let-rachloride $(CCl_4)$ intoxicated rats. Alnus japonica Steud was extracted with methanol and fractionated with hexane, chloroform, ethylacetate, butanol and water. Rats were treated with those orally once a day for 6 days. The activities of aminotransferase and ${\gamma}-glutamyltranspeptidase$ and contents of cholesterol, TG and hepatic lipid peroxide in butanol fraction pretreated rats were significantly decreased compared to the only $CCl_4$ treated rats but the content of glutathione was significantly increased compared to the only $CCl_4$ treated rats. Also activities of hepatic superoside dismutase, catalase, glutathione perpxidase in butanol fraction pretreated rats were signigicantly decreased compared to the only $CCl_4$ treated rats. These result indicated that butanol fraction of Alnus japonica Steude showed hepatoprotective effect in carbon tetrachloride intoxicated rats.
Keywords
carbon tetrachloride; Alnus japonica Steude; hepatoprotective effect;
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1 Lee, Y. N. (1998) Flora of Korea. p. 64. Kyo Hak Publishing Co., Seoul
2 Lee, S. J. (1966) Korea Folk Medicine. p40. Seoul National University Publishing Center Seoul
3 Lee, M. W., Jeong, D. W., Lee, Y A., Park, M. S. and Toh, S. H. (1999) Flavonoids from the leaves of Alnus hirsuta. Yakhak Hoeji 43: 547-552
4 Hirata, T., Ideo, R. and Suga, T. (1977) Structure of alnuselide, the first reported naturally occuring C-31 secodammarane type triterpene lactone from Alnus serrulatoides. Chem. Lett. 8: 711-719
5 Lee, M.W., Tanaka, T., Nonaka, G. I. and Nishioka, 1. (1992) Dimeric ellagitannins from Alnus japonica. Phytochem. 31: 2835-2842   DOI   ScienceOn
6 Sheth, K., Bianchi, E., Wiedhopf, R. and Cole, J. R. (1973) Antitumor agents from Alnus oregona. J. Pharm. Sci. 62: 137-142   DOI   PUBMED
7 Lee, Y. A., Kim, K. H., Kim, J. S., Cho, S. M., Kim, S. W. and Lee, M. W. (2000) Antioxidative effects of diarylheptanoids from Alnus hirsuta. Yakhak Hoeji 44: 193-196
8 Lee, M. W., Kim, J. H, Jeong, D. W., Ahn, K. H. Toh, S. H and Surh, Y J. (2000) Inhibition of cyclooxygenase-2 expression by diarylheptanoids from the bark of Alnus hirsuta Var. sibrica. BioI. Pharm. Bull. 23: 517-518   DOI   ScienceOn
9 Rao, V .C. and Nehendale, H. M. (1991) Colchicine antimiosis abolishes $CCI_4$ autoprotection. Toxicol. Pathol. 19: 597-606   DOI   ScienceOn
10 Kim, O. K. (2000) Protective effects of Houttuynia cordata Thunb on carbon tetrachloride induced hepatotoxicity in rats. kor. J. Phamacogn. 33: 324-331
11 Ha, T. Y, Cho, I. J. and Lee, H. Y (2001) Effect of Cassia tora ethanol extracts on carbon tetrachloride induced liver injury in rats. Korean J. Food Sci. TechnoZ. 33: 789-794
12 Han, E. G. and Cho, S. Y (1997) Effect of codonopsis Ianceolata water extract on the activies of antioxidative enzymes in carbon tetrachloride treated rats. J. Korean Soc. Food Sci. Nutr. 26: 1181-1186;
13 Mates, J. M., Perez, C. and Castro, I. (1999) Antioxidant enzymes and human diseases. Clin. Biochem. 32: 595-   DOI   ScienceOn
14 Friedberg, T., Bentley, P, Stasiecki, P, Glatt, H. R., Raphael, D. and Oesch, F. (1979) The identification, solubilization and characterization of microsome associated glutathione-stransferase. J. BioI. Chem. 254: 12028-12034
15 Asakawa, Y (1971) Chemical constituents of Alnus sieboldiana (Betula ceae) II. The isolation and structure of flavonoids and stibenes. Bull. Chem. Soc. Jpn. 44: 2761-2767   DOI
16 Burkitt, M. J. and Duncan, J. (2000) Effects of trans resveratrol on copper dependent hydroxyl radical formation and DNA damage: evidence for hydroxyl radical scavening and a novel, glutathione sparing mechanism of action. Archives of Biochem. Stry. and Biophysics 381: 253-263   DOI   ScienceOn
17 Prohaska, J.P and Ganther,H. E. (1977) Glutathione peroxidase activities of glutathione-s-transferase purified from rat liver. Biochem. Biophys. Res. Comm. 76: 487-492
18 Kim, S. Y, Lee, H. S., Ryu, K. S., Lee, E. J. and Kim, Y .C. (1999) Protective effects of extracts of Mori cortex Radicis on carbon tetrachloride induced hepatotoxicity. Yakhak Hoeji. 43: 391-396
19 Jungyakdesajon (1985) Sohakkyan. p. 413-415. Sanghae Science pub
20 Kim, T. H. and Park, J.Y (1997) Effect of Cyperi rhizoma on $CCI_4$ induced hepatotoxicity and lipid peroxidation. Kor. J. Pharmacogn. 28: 185-191
21 Butler, T. C. (1990) Reduction of carbon tetrachloride in vivo and reduction of carbon tetrachloride and chloroform in vitro by tissues and constituents. J. Phannacol. Exp. Ther. 143: 311-319
22 Middleton, E. (1996) Biological properties of plant flavonoids an overview. Int. J. Pharmcognosy 34: 344-348   DOI   ScienceOn
23 Kang, C. H., Kim, D. H., Ryu, S. Y and Kim, S. H. (2000) Study on hepatoprotective and antioxidant activities of korean red ginseng mixed formula. Kor. J. Pharmacogn. 31: 383-389
24 Groot, H. and Noll, T. (1986) The crucial role of oxygen partial pressures in haloalkane free radical mediated lipid peroxidation. Biochem. Pharmacol. 35: 15:19   DOI   ScienceOn
25 Aykac, G. (1985) The effects of chronic ethanol indigestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats.Toxicol. 35: 71-79
26 McCay, P.B., Lai, E. K., Poyer, J.L., Dubose, C. M. and Janzen, E. G (1984) Oxygen and carbon-centered free radical formation during carbon tetrachloride metabolism. J. Biol. Chem. 259: 2135-2139
27 Borek, C. (2001) Antioxidant health effects of aged garlic extract. The Journal of Nutrition 131: 101Os-1025s   DOI
28 Lee, M. W., Kim, K. H., Park, M. S., Jeong, D. W. and Toh, S. H (2000) Diarylheptanoids from the leaves of Alnus hirsuta Turez. Arch. Pharm. Res. 23: 50-53   DOI   ScienceOn
29 Hirata, T., Murai, K., Ideo, R. and Suga, T. (1976) Triterpenoids in the male flower of Alnus serrulatoides. prosymp. Chem. Nat. Prod. 3: 273-279
30 Recknagel, R. O., Glende, ,E..A. .and Hruszkewyez, A. M. (1997) Chemical mechanism in carbon tetrachloride toxicity. In free radicals in biology. Prayor, W. A (ed), Academic Press, New York, p. 97-115
31 Deisseroth, A. and Dounce, A. L (1970) Catalase physical and chemical properties, mechanism of catalysis and physiological role. Physiol. Rev. 50: 3-24
32 Tori, M., Hashimoto, A., Hirose, K. and Asakawa, Y (1995) Diarylhep tanoids, flavonoids, stilbenoids, sesquiterpenoids and a phenanthrene from Alnus maximowiczii. Phytochem. 40: 1263-1269   DOI   ScienceOn
33 Recknagel, R. O. (1976) Carbon tetrachloride hepatotoxicity. Pharmac ol. Rev. 19: 145-164
34 Chung, C. S., Woo, B. H., Lee, E. B. and Jung, K. H(1996) The effect of Alnus japonica Cortex extract on gastric lesion and ulcer of rats. J. Applied Pharmacology 1: 84-88
35 Khvorst, O. P, Serbin, A. G., Komissarenko, N. F. and Goldienko, V. G. (1989) Ellagitannins derived from Alnus glutinosa(L). Khim Farm Zh. 23: 45-51
36 Kuresh, A. Y and James, A. J. (2001) A possible emerging role of phytochemicals in improving age-related neurological dysfunctions: A multiplicity of effects. Free Radical Biology and Medicine 30: 583-594   DOI   ScienceOn