• Food Science and Biotechnology
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• 제17권5호
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• pp.1060-1065
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• 2008

A novel benzoyl glucoside (4) and 13 known phenolic compounds were isolated from the leaves of Camellia japonica by a guided 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The structure of 4 was determined to be 4-hydroxy-2-methoxyphenol 1-O-$\beta$-D-(6'-O-p-hydroxylbenzoyl)-glucopyranoside (camelliadiphenoside). The 13 known compounds were identified as (E)-coniferyl alcohol (1), (-)-epicatechin (2), 4-hydroxyphenol 1-O-$\beta$-D-(6-O-p-hydroxybenzoyl) glucopyranoside (3), naringenin 7-O-$\beta$-D-glucopyranoside (5), quercetin 3-O-$\beta$-L-rhamnopyranosyl(1$\rightarrow$6)-$\beta$-D-glucopyranoside (6), kaempferol 3-O-$\beta$-L-rhamnopyranosyl(1$\rightarrow$6)-$\beta$-D-glucopyranoside (7), (+)-catechin (8), 1,6-di-O-p-hydroxybenzoyl-$\beta$-D-glucopyranoside (9), phloretin 2'-O-$\beta$-D-glucopyranoside (10), quercetin 3-O-$\beta$-D-glucopyranoside (11), quercetin 3-O-$\beta$-D-galactopyranoside (12), kaempferol 3-O-$\beta$-D-galactopyranoside (13), and kaempferol 3-O-$\beta$-D-glucopyranoside (14). Their chemical structures were determined by the spectroscopic data of fast atom bondardment mass spectrometry (FABMS) and nuclear magnetic resonance (NMR). Flavonoids having the catechol moiety showed significantly higher DPPH radical scavenging activity than other isolated compounds having monohydroxy phenyl group.

• 한국산림과학회지
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• 제9권1호
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• pp.75-80
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• 1969

우리나라 남부지방(南部地方)에 많이 분포(分布)되는 동백나무에 대(對)한 개화기인산(開花期燐酸) 효율(効率)과 체내행동(體內行動)에 대(對)하여 $P^{32}$ 표식인산(標識燐酸)을 사용(使用)하여 추적(追跡)하여 본바 다음과 같은 결과(結果)를 얻었다. 1. Flower-bud의 P%는 다른 부분(部分)의 약(約) 2배치(倍値)을 시현(示顯)하였고 Root와 Stem은 다같이 동일(同一)한 경시적(經時的) 변화상(變化相)을 나타내였다. 2. 체내이동(體內移動)은 전반적(全般的)으로 봐서 24시간(時間) 내(內)에 일어나는 것 같았으며 인산(燐酸) 함량비(含量比)는 Growing-Point와 Flower-bud가 나머지 3부분(部分)의 합계(合計)와 같은 절반치(切半値)를 점유(占有)하였다. 3. 동백나무의 부분별(部分別) 비방사능(比放射能)은 Growing-point가 가장 현저(顯著)하였고 48시간(時間) 이후(以後)에는 평균(平均) 80%이상(以上)을 해부분(該部分)이 차지하였으며 본(本) 실험(實驗) 결과(結果)에서 얻어진 인산(燐酸)의 효율도(効率度)(이용도(利用度))는 약(約) 19% 정도(程度)로 나타 났다.

• Journal of Ecology and Environment
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• 제38권3호
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• pp.327-334
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• 2015

We investigated the current and potential spatial distributions and habitable areas of Biston robustum and Camellia japonica in South Korea in order to provide useful data for the conservation of C. japonica and minimize the damage caused by B. robustum. It was predicted that, by 2070, although B. robustum would be widely distributed throughout the Korean Peninsula, except for the western and eastern coastal areas, it would be narrowly distributed along the Sokcho-si and Goseong-gun coastlines in Gangwon Province. C. japonica is currently located along the southern coastline but its critical habitable area is predicted to gradually disappear by 2070. Assessment of the potential distribution probabilities of B. robustum and C. japonica revealed that the area under the curve (AUC) values were 0.995 and 0.991, respectively, which indicate high precision and applicability of the model. Major factors influencing the potential distribution of B. robustum included precipitation of wettest quarter and annual precipitation (BIO16 and BIO12), whereas annual mean temperature and mean temperature of wettest quarter (BIO1 and BIO8) were important variables for explaining C. japonica distribution. Overlapping areas of B. robustum and C. japonica were $11,782km^2$, $5447km^2$, and $870km^2$ for the current, 2050-predicted, and 2070-predicted conditions, respectively, clearly showing a dramatic decrease in area. Although it is predicted that B. robustum would cause continuous damage to C. japonica in the southern part of the Korean Peninsula, such impacts might diminish over time and become negligible in the future.

• 대한의생명과학회지
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• 제12권3호
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• pp.281-287
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• 2006

Cigarette smoke causes atypical structure of pulmonary and oxidative damage. Therefore, we carried out to determine if exposure to cigarette smoke alters pulmonary structure and anti-oxidant related enzyme in a animal model, when natural product extracts using by Nebulizer. The rat were divided into four groups: $H_2O-treated$ (Control), natural product (Camellia sinensis) extracts-treated (CS), natural product extracts-treated with cigarette smoke-exposed (CS+SM) and cigarette smoke-expose (SM). All groups are similar to Control group in weight, but SM group is lower than the other groups. Microscopic image of the pulmonary structure in SM group showed deleterious alterations in the morphology, but the other groups are maintained in normal structure. In anti-oxidant related enzymes, SOD (superoxide dismutase) and catalase, SM group represents the lowest enzyme activity among all groups. But G6PD (glucose-6-phosphate dehydrogenase) and LPO (lipid peroxidation) is SM group represents the highest enzyme activity among all groups. These result indicate that the natural product extracts is an efficient tissue protective substance against smoke-induced lung injury.

• Biomolecules & Therapeutics
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• 제24권6호
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• pp.630-637
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• 2016

The chemical components and biological activity of Camellia mistletoe, Korthalsella japonica (Loranthaceae) are relatively unknown compared to other mistletoe species. Therefore, we investigated the phytochemical properties and biological activity of this parasitic plant to provide essential preliminary scientific evidence to support and encourage its further pharmaceutical research and development. The major plant components were chromatographically isolated using high-performance liquid chromatography and their structures were elucidated using tandem mass spectrometry and nuclear magnetic resonance anlysis. Furthermore, the anti-inflammatory activity of the 70% ethanol extract of K. japonica (KJ) and its isolated components was evaluated using a nitric oxide (NO) assay and western blot analysis for inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. Three flavone di-C-glycosides, lucenin-2, vicenin-2, and stellarin-2 were identified as major components of KJ, for the first time. KJ significantly inhibited NO production and reduced iNOS and COX-2 expression in lipopolysaccharide-stimulated RAW 264.7 cells at $100{\mu}g/mL$ while similar activity were observed with isolated flavone C-glycosides. In conclusion, KJ has a simple secondary metabolite profiles including flavone di-C-glycosides as major components and has a strong potential for further research and development as a source of therapeutic anti-inflammatory agents.

• 대한가정학회지
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• 제43권8호
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• pp.1-12
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• 2005

Ash solutions derived from vegetation have been known for their good mordanting action. They are used as finishing agents because they are able to promote dye uptake. It is expected that the types of ash solutions may have been different worker by worker since the workers have employed easy-to-get plants, when we reviewed old literature. However, the ash solutions are being used without clear distinctions between their characteristics. In this study, camellia ash solution and rice straw ash solution were prepared according to traditional methods. Using these solutions as mordanting agents, natural dyeing experiments with Caesdpinia Sappan were peformed. Following the dyeing procedure, the effect of the type of ash solution on the color of the dyed fabric was evaluated. The results showed that the ash solution mordanting effect us not dependent upon the components of the ash solution. It was also demonstrated that the mordanting effect was not based on the action of the metallic ions in the ash solution. The pH values of the ash solutions were adjusted in order to investigate the effect of changes in ash solution alkalinity on the dyeing process. The pH values ranged from neutral or weak acidity to the alkaline range in order to evaluate the characteristics of the mordanting tv the ash solutions.

• 한국환경생태학회지
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• 제20권2호
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• pp.81-93
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• 2006

월출산국립공원 전 지역을 대상으로 146개의 조사구를 설치하여 상록활엽수 개체군 동태를조사한 결과, 월출산국립공원내 자생하는 것으로 확인한 상록활엽수종은 총 13종이었고, 주요 곡간부를 중심으로 최대 해발 450m까지 분포하는 것으로 조사되었다. 월출산국립공원에 자생하는 상록활엽수중 동백나무, 사스레피나무, 붉가시나무는 식물군락을 이루며 분포하였으나, 참식나무, 모새나무, 차나무, 보리밥나무, 광나무, 자금우는 반상이나 단독 형태로 분포하였다. 지피식물인 마삭줄, 백화등, 송악은 국지적으로 희소하게 출현하였다. 월출산국립공원의 일부 지역에서 소규모 형태로 붉가시나무림이 분포하였고, 상록활엽수들은 소나무군락, 굴참나무군락, 상수리나무군락의 임상층에서 주로 분포하고 있었다. 붉가시나무군락은 그 세력이 확장될 것으로 생각되며, 붉가시나무가 생육하고 있는 소나무군락과 낙엽활엽수림군락들은 붉가시나무군락으로 식생천이가 예상된다.

• 한국환경생태학회지
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• 제23권3호
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• pp.272-279
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• 2009

본 연구는 부산시 도로변에 식재되어 있는 생울타리의 종과 잎의 두께에 따른 소음 감소효과를 분석하고자 하였다. 잎의 두께만으로 소음감소 효과를 분석한 결과 은목서, 동백나무, 피라칸사, 홍가시, 돈나무, 남천, 사철나무, 명자나무, 금식나무 순이었다. 수목별 잎의 소음감소효과 분석 결과 대부분의 수종은 잎의 두께가 두꺼운 잎이 얇은 잎보다 소음 감소효과가 더 있는 것으로 조사되었으며, 선형회귀 분석결과 회귀식은 설명력 $R^2$이 0.385로 다음과 같은 회귀식(Y = 7.653 + 26.530 X)을 얻었다. 생울타리의 소음 감소효과를 분석한 결과 동백나무, 남천, 돈나무, 주목, 명자나무 순으로 조사되었고, 가장 소음감소 효과가 높은 수목은 14.70[d8]을 보인 동백나무였으며, 가장 소음 감소치가 낮은 수목은 6.80[dB]을 보인 명자나무로 7.9[dB]의 차이를 보였다.

• 한국자원식물학회지
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• 제18권2호
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• pp.351-358
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• 2005

차나무 카테킨류의 생산성을 높이기 위하여 차나무 잎의 캘러스 배양시 thiamine-HCl과PVP의 효과를 조사하였다. 캘러스의 생장은 20 mg/L thiamine-HCl이 포함된 캘러스증식배지(0.2 mg/L 2,4-D와 1.0 mg/L TDZ가 첨가된 MS 배지)에서 가장 왕성하여 대조구에 비해 1.7배의 생장을 보였다. 배양된 캘러스의 카페인 함량은 신초에 비해 $10\%$이하로 낮아졌고 총 카테킨류 함량은 40 mg/L thiamine-HCl에서 가장 높아 신초의 1.6배까지 증가하였다. 특히 신초에서는 검출되지 않았던 (-)-epicatechin(EC)이 thiamine-HCl 또는 PVP를 처리하여 배양하였을 경우 모든 처리에서 검출되었으며, (-)-epicatechingallate(ECG)는 신초에서 보다 4.8배나 높은 함량을 나타내었다. 캘러스 생장과 고효율의 카테킨 생산을 고려했을 때 30 mg/L thiamine-HCl이 포함된 배지가 최적의 배양조건으로 판단된다.

• Food Science and Biotechnology
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• 제18권3호
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• pp.766-770
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• 2009

Hot water extracts of Camellia japonica flower buds were found to have the higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than the other solvent extracts. Five phenolic compounds were isolated and purified from the ethyl acetate soluble-neutral fraction of hot water extracts by Sephadex LH-20 column chromatography and octadecyl silane-high performance liquid chromatography using the guided assay of DPPH radical scavenging. Based on mass spectrometer and nuclear magnetic resonance, the isolated compounds were identified as p-hydroxybenzaldehyde (1), vanillin (2), dehydroxysynapyl alcohol (3), 7S,7'S,8R,8'R-icariol $A_2$ (4), and (-)-epicatechin (5). Four compounds (1-4) except for 5 were newly identified in this plant. Their DPPH radical scavenging activities as 50% scavenging concentration decreased in order to 5 $(20\;{\mu}M)>{\alpha}-tocopherol$ $(29\;{\mu}M)>4$ $(67\;{\mu}M)>3$ $(72\;{\mu}M)>1=2$ ($>250\;{\mu}M$). These results indicate that the antioxidant effect of the hot water extract of C. japonica flower buds may partially due to 5 isolated phenolic compounds.