• Korean Journal of Plant Resources
• /
• v.21 no.2
• /
• pp.134-138
• /
• 2008

Nerine from south africa and its sparkling flower shape make us estimate it as a hopeful kind of cut follow. There was a few studies on Nerine in korea. We started this study to set bulb propagation methods. The propagation by tissue culture was changeable according to the growth regulators The best growth regulator combination which makes a lot of Bulblet was NAA $0{\sim}0.5$ + BA $0.5{\sim}2.0mg\;{\cdot}\;L^{-1}$ in Nerine bowdenii ‘Favourite’ and Nerine sarniens ‘Red’ respectively. The adjust culture media source for tissue culture were glucose 9% as a carbon source and ($NH_4+NO_3$) 40mM as a nitrogen source. When glucose was used as a carbon source, Bulblet were harvested a little bit low then sucrose but comparative emergence rate was so high that it is good for carbon source in nerine tissue culture. When we consist culture media as MS+BA $1.0mg\;{\cdot}\;L^{-1}$+sucose 7% + ($NH_4+NO_3$) 40mM, the produced Bulblet were reached up to 1.7 each per bulb and emergence rate was up to 100% irrespective of acclimatization period. The suitable culture explant for nerine tissue culture was scale. When scale was cultured with MS+BA $1.0mg\;{\cdot}\;L^{-1}$+sucose 7%, its propagation efficiency was 54 times greater than using growing point. A proper culture part of the scaly leaf was middle part (8 scaly leaf from outer 8th scaly leaf) when middle part was cultured the number of Bulblet were up to 1.8 each per explant.

• Journal of Biosystems Engineering
• /
• v.17 no.1
• /
• pp.55-64
• /
• 1992

The aim of this study was to develop a phytotron for studying the effects of environmental factors such as temperature and humidity on plant growth. This equipment consists of the growth chamber, and the measurement and control system including control algorithms required for optimum operation. As the first step of the study, a temperature and humidity control system was developed. The results of this study are summarized as follows ; 1. Pt-100 was selected to measure temperature and a linearized op-amp circuit was developed for signal conditioning. 2. Pt-100 wet bulb thermometer based on Asmann's principle was developed to measure relative humidity. 3. Temperature and relative humidity conditions were controlled by ON-OFF and PWM operation using a PID controller. And an autotuning algorithm using the characteristics of step response was developed to determine optimal PID constants which were independent of the size of apparatus and environmental factors. 4. Under the ambient temperature of $20^{\circ}C{\sim}25^{\circ}C$, the temperature was kept within the error of ${\pm}0.3^{\circ}C$ in the range of $10^{\circ}C{\sim}40^{\circ}C$, and the relative humidity was kept within the error of ${\pm}5%$ in the range of ${\pm}50%{\sim}90%$.

• Journal of Microbiology and Biotechnology
• /
• v.22 no.12
• /
• pp.1776-1781
• /
• 2012

Multiple viruses such as Lily symptomless virus (LSV), Lily mottle virus (LMoV), and cucumber mosaic virus (CMV) are the most prevalent viruses infecting lilies in Korea. Leaf samples and bulbs showing characteristic symptoms of virus infection were collected from Gangwon, Chungnam, and Jeju provinces of Korea in 2008-2011. Coat protein (CP) genes of LSV and LMoV were amplified from collected samples by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into a pET21d(+) expression vector to generate recombinant CPs. The resulting carboxy-terminal His-tagged CPs were expressed in Escherichia coli strain BL21(DE3) by isopropyl-1-thio-${\beta}$-D-galactoside induction. The recombinant proteins were purified using Ni-NTA agarose beads, and the purified proteins were used as an immunogen to produce polyclonal antibodies in rabbits. The resulting polyclonal antisera recognized specifically LSV and LMoV from infected plant tissues in Western blotting assays. Indirect enzymelinked immunosorbent assay and immunocapture RT-PCR using these polyclonal antisera were developed for the sensitive, efficient, economic, and rapid detection of Lily viruses. These results suggest that large-scale bulb tests and economic detection of Lily viruses in epidemiological studies can be performed routinely using these polyclonal antisera.

• Korean Journal of Agricultural Science
• /
• v.46 no.2
• /
• pp.341-350
• /
• 2019

Garlic is one of the most popular seasoning bulb vegetables in Korea and is the most commonly used food ingredient. However, the cultivation areas are decreasing every year as the price drops due to imported garlic, and labor is insufficient to produce garlic by conventional methods. Cultivation requires various tasks until garlic is harvested. Seeding is one of the important and laborious tasks; thus, mechanization is necessary. When seeding garlic, the sprout should face upwards; otherwise, it may rot or produce poor quality garlic. This study investigated the extent of growth of northern- and southern-type garlic in eight different positions. The results show when the roots were not planted properly, the stems were weak, and the garlic bulbs were small. A simple garlic planter was manufactured with a crank-press mechanism to plant garlic in an upright position. Using this machine, a three-fold experiment was carried out with 100 strips of garlic. The test results showed that 99.4% of the planted garlic strips were positioned upright or close to being upright, and 0.6% failed to take root. An image processing algorithm was developed to locate the root part of the garlic, and the result showed a success rate of 81%. A future study will develop an automatic garlic upright planting system with a viewing system.

• Horticultural Science & Technology
• /
• v.19 no.4
• /
• pp.471-475
• /
• 2001

This experiment was conducted to evaluate the effect of slow-release fertilizer on warm type garlic cultivation in the Southwestern region of Korea. The slow-release fertilizers used were UF(Urea-Formaldehydes)70, 100, 130, CDU(Crotonylidene Diurea)100, and IBDU(Isobuty- lidene Diurea)100. The slow-release fertilizer treatment slightly increased plant height, leaf sheath diameter, and leaf number compare with conventional fertilizer treatment. Also, large bulb rate, nitrogen and pyruvic acid contents of garlic in slow-release fertilizer plot were higher than other treatments. Application of slow-release fertilizer increased the commercial yields.

• Korean Journal of Plant Tissue Culture
• /
• v.24 no.2
• /
• pp.103-106
• /
• 1997

In order to establish a micropropagation system of Naricissus, the ability of bulblet regeneration among propagation materials was compared, and the adequate growth regulators and concentrations for each cultivar were investigated. The inorganic components were also assayed in the parts of propagation materials. In propagation materials, scape with based plates showed hightest rate of bulblet formation and rapid growth of formed bulblets in vitro, comparing to other parts of it. In comparing of varieties, 'Dutch Master' and 'Golden Harvest' showed a high ability for bulblet regeneration. The ability of bulblet regeneration was most favorable in the medium, supplemented with 5.0 mg/L BA and 2.5 mg/L NAA in 'Dutch Master', and 5.0 mg/L BA and 1.0mg/L NAA in 'Golden Harvest', respectively. In inorganic component analysis of propagation materials, the White part of scape contained 1.18 mg/L$P_2O_5$, 2.57 me Ca, 0.94 me Mg and 3.20 mg/L total N. It showed higher levels in concentration of inorganic components as compared to those of the other part of scape. In addition, leaves and yellow part of scape contained significantly high levels of Ca and Mg while scales bulb showed considerably low levels in all inorganic compounds.

• Current Research on Agriculture and Life Sciences
• /
• v.29
• /
• pp.21-27
• /
• 2011

This study was carried out to produce multiple shoots and bulblets from flower stalk tissue cultures of Muscari armeniacum LEICHTLIN ex BAKER 'Early Giant', which were cultured in the half strength Murashige & Skoog's (MS) medium supplemented with auxin, NAA in combination with kinetin, BA, and TDZ, alone and/or. In flower stalk tissue culture, upper part explant was the most suitable as a source of culture material. Direct shoot formation was much more favorable in half strength MS medium supplemented with $0.1mg{\cdot}L^{-1}$ NAA and $1.0mg{\cdot}L^{-1}$ TDZ. On the other hand, bulblet formation was increased when cultured in half strength MS medium added with $0.01mg{\cdot}L^{-1}$ NAA and $0.1mg{\cdot}L^{-1}$, $0.2mg{\cdot}L^{-1}$ kinetin. Acclimatized plant flowered during the second year of the growing period without any phenotypic variations and formed average 1.5 bulblets per mother bulb.

• Journal of Plant Biotechnology
• /
• v.31 no.3
• /
• pp.197-201
• /
• 2004

Shoot clusters were induced from bulb scales of Lilium longiflorum 'Geogia', and proliferated on medium containing 0.5 mg/L BA and 0.5 mg/L IAA. Thereafter, these shoot clusters were cultured in 5 L air-lift bioreactors to form and grow normal bulblets. Number of bulblets increased on medium with 30 g/L sucrose, but growth of bulblets was effective on medium with 60 g/L sucrose. The number of bulblets from shoot clusters had no differences, though bulblet growth was very effective on medium with between full and double strength of MS salts. The inoculation of 100 g shoot clusters as a cultural material was suitable for formation and growth of bulblets in 5 L bioreactors. Air-lift type was more effective for the formation and growth of bulblets than that in ebb and flood one, and 200∼300 mL$.$min$^{-1}$ injection of air was suitable in growth of bulblets.

• Research in Plant Disease
• /
• v.17 no.2
• /
• pp.222-224
• /
• 2011

The sclerotium rot of onion (Allium cepa L.) was occurred sporadically in the farmer's fields at Daehap, Changnyeong in Korea. The typical symptom was water-soaking on the bulb, stem and leaves and then rotting, wilting, blighting and the infected plants eventually were rotted and died. The sclerotia were globoid, 1-3 mm, and white to brown. The optimum temperature for mycelial growth and sclerotial formation on PDA was $30^{\circ}C$. The hyphal width was 4-9 ${\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. On the basis of mycological characteristics, ITS rDNA sequence analysis, and pathogenicity, this fungus was identified as Sclerotium rolfsii Saccardo. To our knowledges, this is the first report of sclerotium rot on onion caused by S. rolfsii in Korea.

• Korean Journal of Plant Resources
• /
• v.9 no.3
• /
• pp.274-278
• /
• 1996

This study was conducted to examine the dry matter production and leaf photosynthesis under different fertilizer application in Yacon(Polymnia sonchifolia)plants. Under field conditions, dry matter production of stable mature application plot(S-1) was much larger than non-treated control plot(CTL), but bulb dry weight of 10kg nitrogen application plot per 10a (N-10) was not differentiated from CTL. The maximum photosynthetic rates increased in Yacon plants grown at S-1 while in the rates of CTL reduced under glasshouse in the same treated pot condition. The optimum temperature for the highly photosynthetic rates is about $34{\pm}3^{\circ}C$, and the rates did decrease by stomatal resistance below 28℃. The estimated light saturation point was $1200{\mu}mol{\cdot}m^{-2}s^1$. The results of this study indicate that soil conditions were highly related to dry matter production and leaf photosynthesis rate in Yacon plants.