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http://dx.doi.org/10.5010/JPB.2004.31.3.197

Micropropagation of Lilium longiflorum 'Geogia' by Using Bioreactor.  

Han, Bong-Hee (원예연구소 원예생명공학과)
Suh, Eun-Jung (원예연구소 원예생명공학과)
Yae, Byeoung-Woo (농촌진흥청 연구관리과)
Yu, Hee-Ju (원예연구소 원예생명공학과)
Publication Information
Journal of Plant Biotechnology / v.31, no.3, 2004 , pp. 197-201 More about this Journal
Abstract
Shoot clusters were induced from bulb scales of Lilium longiflorum 'Geogia', and proliferated on medium containing 0.5 mg/L BA and 0.5 mg/L IAA. Thereafter, these shoot clusters were cultured in 5 L air-lift bioreactors to form and grow normal bulblets. Number of bulblets increased on medium with 30 g/L sucrose, but growth of bulblets was effective on medium with 60 g/L sucrose. The number of bulblets from shoot clusters had no differences, though bulblet growth was very effective on medium with between full and double strength of MS salts. The inoculation of 100 g shoot clusters as a cultural material was suitable for formation and growth of bulblets in 5 L bioreactors. Air-lift type was more effective for the formation and growth of bulblets than that in ebb and flood one, and 200∼300 mL$.$min$^{-1}$ injection of air was suitable in growth of bulblets.
Keywords
Bioreactor; bulblet growth; Lilium; MS salt; shoot cluster; sucrose;
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