• Journal of Embryo Transfer
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• v.27 no.1
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• pp.37-43
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• 2012

This study evaluated a method of sorting X and Y chromosomes based on size using the forward angle light scatter related refractive index (FSC) of a flow cytometer. Hanwoo bulls sperm were separated to X and Y chromosomes by the parameters of FSC or Hoechst 33342 intensity. As a result, using monitor program linked flow cytometry during sorting processing, the purities were $97{\pm}0.57$ or $96{\pm}0.67%$ for the X-fraction and $96{\pm}0.33$ or $97{\pm}1.33%$ for the Y-fraction in the two sperm sorting methods. There were no differences in the X and Y ratios (X and Y %) between the sperm sorting methods based on FSC or DNA content. The proportions of female and male embryos used for in vitro fertilization and development were $66.03{\pm}3.31$ or $69.37{\pm}1.41%$, and $70.56{\pm}2.42$ or $56.11{\pm}3.09%$ when sperm were processed using the sex sorting method by FSC or Hoechst 33342. In conclusion, further study is needed to determine the optimum procedure and improve the nozzle to enhancing sorting accuracy or efficiency. Also, the findings of this study do not negate the possibility that the difference method of sperm sorting cannot use a UV laser beam.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.21 no.6
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• pp.1490-1500
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• 1996

One of the important issues for multimedia communications over ATM netowrks is efficient use of net-work resources since transmitting video at low cost requires high utilization of channel bandwidth. In this paper, we propose an effective bandwidth estimation scheme for MPEG sources in ATM networks. It has been known difficult to allocate effective transmission bandwidth to MPEG sources due to its bursty charactersitics. The MPEG traffic stream can bemodeled using five traffic parameters:service rate, burst load ratio, interburst load ratio, burst period, and interburst period. Using these parameters, a transmission bandwidth for each source can be estimated, and the estimated bandwidth is applied synchronously to reduce the queue size in a buffer. For a simple network modelof a high speed link that multiplexes a number of virtual-circuit connections, simulations on adaptive bandwidth allocation were performed, and the results show that the queueing delay is significantly reduced, when compared to a fixed bandwidth allocation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.7A
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• pp.964-972
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• 1999

In this paper, we proposed a simulation model to which apply the AAL 2 (AAL type 2) between BSC and MSC in the cellular mobile communication systems. We suggested the frame structure of processing the packets of short length and the scheme which multiplex to one or more ATM cell. Also, we analyzed the performance of the APR, transmission delay, and channel transmission efficiency used in the packetized voice traffic and the low bit-rate data traffic such as fax. From the simulation results, the maximum number of users are 47 users without using AAL 2 multiplexing, but the maximum number of users are 70 (Non-Overlapping scheme) users, 110 (Overlapping scheme) users, respectively. Thus, we knew that the Overlapping scheme is more efficient than the Non-Overlapping scheme. Finally, we showed that the optimum transmission buffer size is 4 ATM cells in the cellular communication systems with the bandwidth of 2 Mbps.

• YAKHAK HOEJI
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• v.55 no.1
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• pp.1-10
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• 2011

Mastic is a bleed resin formed in pistacia lentiscus tree extract form the anacatdiaceae family. Mastic is used as a food ingredient in the Mediteraanean resin, and has been used by local inhabitants as a traditional medicine for relief of upper abdominal discomfort, dyspepsiaand peptic ulcer. Clinically, mastic has been effective in the treatment of benign gastric and duodenal, ulcers, giving symptomatic relief and endoscopically proven healing. In this study, to enhance activiteies of poorly water soluble Mastic with oils, surfactants and cosurfactants and then the mixure was microemulsified in aqueous media under condition of gentle agitation and digestive motility that would be encountered in the gastrointestinal tract. Formulation development and screening were based on phase diagrams and characteristics of resultant microemulsion. For optimum mastic formulation, microemulsions with various ratio (w/w%) of mastics, oils, surfactants and cosurfactants were prepared and their solubility was evaluated by monitoring particles size in their buffer through visual asessment and electrophoretic light scattering spectrophotomerter (ELS). In vitro activity of self microemulsified mastic (SME mastic) was determined by minimum ingibition concentration (MIC) test against a panel of Helicobacter pylori (H. pylori) clinical strains. Additionally, in vivo activity of SME masitc was investigated us mouse infected by CH275 of H. pylori. The mean diameter of SME mastic was less then 100 nm in water and SME mastic was showed similar antiboisis effect compared to tometronidazole, clarithromycin and omeproazole. Consequently, SME mastic would be effective system to exterminate H. pylori. If mastic were dose with combined treatment, mastic might augur well for effect of H. pylori eradication as good remedy.

• Bulletin of the Korean Chemical Society
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• v.29 no.5
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• pp.953-958
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• 2008

A novel anthraquinone diamino-bridged bis($\beta$ -cyclodextrin) 2 was synthesized. The inclusion complexation behaviors of the native $\beta$ -cyclodextrin 1 and the novel bis($\beta$ -cyclodextrin) 2 with guests, such as acridine red (AR), neutral red (NR), ammonium 8-anilino-1-naphthalenesulfonate (ANS), sodium 2-(p-toluidinyl) naphthalenesulfonate (TNS) and rhodamine B (RhB) were investigation by fluorescence, circular dichroism and 2D NMR spectroscopy. The spectral titrations were performed in phosphate buffer (pH 7.20) at 25 ${^{\circ}C}$ to give the complex stability constants (Ks) and Gibbs free energy changes (−${\Delta}G^0$) for the stoichiometric 1:1 inclusion complexation of host 1 and 2 with guests. The results indicated that the novel bis($\beta$ -cyclodextrin) 2 greatly enhanced the original binding affinity of the native $\beta$ -cyclodextrin 1. Typically, bis($\beta$ -cyclodextrin) 2 showed the highest binding constant towards ANS up to 34.8 times higher than that of 1. The 2D NMR spectra of bis($\beta$ -cyclodextrin) 2 with RhB and TNS were performed to confirm the binding mode. The increased binding affinity and molecular selectivity of guests by bis($\beta$ -cyclodextrin) 2 were discussed from the viewpoint of the size/shape-fit concept and multipoint recognition mechanism.

• Korean Journal of Veterinary Research
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• v.39 no.3
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• pp.513-522
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• 1999

DNA hybridization assay using probes prepared from liver was carried out to identify species characterization of the domestic animals. Gel electrophoresis showed that the target DNA extracted from raw muscle were 1kb and uniform pattern while fragments size of heated muscle were irregular. Hybridization was performed by adding 200ng/ml probe in hybridization solution and incubating for 12 hours at $68^{\circ}C$. To obtain good discrimination, applied washing buffer and washing step differently depending on the species. The probes of pig, horse and dog formed the specific hybrids with each target DNA respectively. Although cross reaction was detected in cattle, goat and sheep but signal intensity among these species made the discrimination possible each other. Such pattern was the same in the cases of chicken, turkey and duck. The hybridization pattern of heated muscle was similar to that of raw muscle in general, but the signal intensity was inferior to that of raw muscle. Species identification between closely related animal species, hybridized using the target DNA of such closely related animal species as a blocking agent, remarkable increase of discrimination from the evident decrease of non specific reaction compared with the control group. In addition, in the admixture where certain meat was included in the beef, pork, chicken meat, we could find whether any unjust meat was admixed or not. In this case, detection limit of certain meat in admixture was 1%.

• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.942-952
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• 2013

Monoliths are functional porous materials with a three-dimensional continuous interconnected pore structure in a single piece. A monolith with uniform shape based on poly(${\gamma}$-glutamic acid) (PGA) has been prepared via a thermally induced phase separation technique using a mixture of dimethyl sulfoxide, water, and ethanol as solvent. The morphology of the obtained monolith was observed by scanning electron microscopy and the surface area of the monolith was evaluated by the Brunauer Emmett Teller method. The effects of fabrication parameters such as the concentration and molecular mass of PGA and the solvent composition have been systematically investigated. The PGA monolith was cross-linked with hexamethylene diisocyanate to produce the water-insoluble monolith. The addition of sodium chloride to the phase separation solvent affected the properties of the cross-linked monolith. The swelling ratio of the cross-linked monolith toward aqueous solutions depended on the buffer pH as well as the monolith fabrication condition. Copper(II) ion was efficiently adsorbed on the cross-linked PGA monolith, and the obtained copper-immobilized monolith showed strong antibacterial activity for Escherichia coli. By combination of the characteristic properties of PGA (e.g., high biocompatibility and biodegradability) and the unique features of monoliths (e.g., through-pore structure, large surface area, and high porosity with small pore size), the PGA monolith possesses large potentials for various industrial applications in the biomedical, environmental, analytical, and separation fields.

• Journal of the Korea Society for Simulation
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• v.22 no.3
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• pp.35-41
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• 2013

This paper applies an active period based bottleneck detection method to flow shop manufacturing system with limited buffer size. Manufacturing systems are constrained by one or more bottlenecks which degrades the system throughput. Conventional bottleneck detection methods include the waiting time or queue length of production stations and their utilization. Due to the random events such as production time of items, machine failure and repair times, the systems may change over time, and subsequently bottlenecks shift from one station to another station. Active period of working station may cause other stations to wait for productions. Information when and where active periods occur helps to find bottlenecks in production systems. Based on these informations, we predict bottlenecks in applying AweSim simulation language. We compare the simulation results of conventional methods with those obtained from duration of active period method, and duration ratio method of both sole and shift bottleneck periods. Even though simulation results are from simple flow shop model, they are quite promising for predicting bottlenecks of production stations. We hope this study aids in decision making regarding the improving system production yield and allocation of available resources of system.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.41 no.3
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• pp.120-128
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• 2018

OLED Display fabrication system is one of the most complicated discrete processing systems in the world. As the glass size grows from $550{\times}650mm$ to $1,500{\times}1,850mm$ in recent years, the efficiency of Automated Material Handling System (AMHS) has become very important and OLED glass manufacturers are trying to improve the overall efficiency of AMHS. Aiming to meet the demand for high efficiency of transportation, various kind of approaches have been applied for improving dispatching rules and facility layout, while simultaneously considering the system parameters such as glass cassettes due date, waiting time, and stocker buffer status. However, these works did not suggest the operational policy and conditions of distribution systems, especially for handling unnecessary material flows such as detour. Based on this motivation, in this paper, we proposed an efficient algorithm for improving detour transportation in OLED FAB. Specifically, we considered an OLED FAB simplifying OLED production environment in a Korean company, where four stockers are constructed for the delivery of Lot in a bay and linked to processing equipments. We developed a simulation model using Automod and performed a numerical experiment using real operational data to test the performance of three operation policies under considerations. We showed that a competitive policy for assigning alternative stocker in case of detour was superior to the current dedicated policy using a specified stocker and other considered policies.

• Journal of the Korean Association of Geographic Information Studies
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• v.7 no.4
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• pp.1-14
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• 2004

In this study, we propose Linktable based on extended TB-Tree(LTB-Tree) which can improve the performance of existing TB (Trajectory-Bundle)-tree proposed for indexing the trajectory of moving objects in GIS Applications. In addition, in order to evaluate proposed indexing scheme, we take into account as follows. At first, we select existing R*-tree, TB-tree, and LTB-tree as the subject of performance evaluation. Secondly, we make use of random data set and real data set as experimental data. Thirdly, we evaluate the performance with respect to the variation of size of memory buffer by considering the restriction of available memory of a given system. Fourth, we test them by using the experimental data set with a variation of data distribution. Finally, we think over insertion and retrieval performance of trajectory query and range query as experimental measures. The experimental results show that the proposed indexing scheme, LTB-tree, gains better performance than traditional other schemes with respect to the insertion and retrieval of trajectory query.