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In vitro and In vivo Activities Studies of Chois Mastic Using Self-microemulsifying Drug Delivery System (SMEDDS) against Helicobactor pylori  

Kim, Su-Ji (College of Medicine, Chungnam National University)
Jeong, Sang-Young (R&D Center Life Science laboratory, Kolma Korea Co., LTD.)
Gil, Young-Sig (R&D Center Life Science laboratory, Kolma Korea Co., LTD.)
Shin, Byung-Cheol (Korea Research Institute of Biomaterials Research Center)
Hwang, Sung-Joo (College of Medicine, Chungnam National University)
Cho, Sun-Hang (College of Medicine, Chungnam National University)
Publication Information
YAKHAK HOEJI / v.55, no.1, 2011 , pp. 1-10 More about this Journal
Abstract
Mastic is a bleed resin formed in pistacia lentiscus tree extract form the anacatdiaceae family. Mastic is used as a food ingredient in the Mediteraanean resin, and has been used by local inhabitants as a traditional medicine for relief of upper abdominal discomfort, dyspepsiaand peptic ulcer. Clinically, mastic has been effective in the treatment of benign gastric and duodenal, ulcers, giving symptomatic relief and endoscopically proven healing. In this study, to enhance activiteies of poorly water soluble Mastic with oils, surfactants and cosurfactants and then the mixure was microemulsified in aqueous media under condition of gentle agitation and digestive motility that would be encountered in the gastrointestinal tract. Formulation development and screening were based on phase diagrams and characteristics of resultant microemulsion. For optimum mastic formulation, microemulsions with various ratio (w/w%) of mastics, oils, surfactants and cosurfactants were prepared and their solubility was evaluated by monitoring particles size in their buffer through visual asessment and electrophoretic light scattering spectrophotomerter (ELS). In vitro activity of self microemulsified mastic (SME mastic) was determined by minimum ingibition concentration (MIC) test against a panel of Helicobacter pylori (H. pylori) clinical strains. Additionally, in vivo activity of SME masitc was investigated us mouse infected by CH275 of H. pylori. The mean diameter of SME mastic was less then 100 nm in water and SME mastic was showed similar antiboisis effect compared to tometronidazole, clarithromycin and omeproazole. Consequently, SME mastic would be effective system to exterminate H. pylori. If mastic were dose with combined treatment, mastic might augur well for effect of H. pylori eradication as good remedy.
Keywords
self-microemulsifying drug delivery system (SMEDDS); mastic; Helicobacter pylori;
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