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Improvement in Storabilities of Rice Bran Protein Film Containing Bacteriocin Produced by Pseudomonas putida 21025 (Pseudomonas putida 21025가 생성하는 bacteriocin을 이용한 항균성 미강 단백질 필름의 저장성 향상)

  • 김은정;김경미;배동호
    • Food Science and Preservation
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    • v.10 no.3
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    • pp.411-416
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    • 2003
  • An effort was attempted to utilize an under-utilized protein source, rice bran protein, in coating or wrapping food material for the purpose of protection them from oxidation and bacterial infection. However, the utilization of rice bran protein as a food coating material is limited because the rice bran protein coating material itself can be spoiled by a bacterial infection. Therefore, this study was conducted to produce the economical and antibacterial rice bran protein film by utilizing rice bran and bacteriocin-producing microorganism. Bacteriocin produced by Pseudomonas putida 21025 was partially purified after 33h of shaking incubation at 30$^{\circ}C$. The amount of amino-type nitrogen did not increase in the rice bran protein film containing the bacteriocin any more after gradual increase upto the content of 0.22% for 8 days, while that without the bacteriocin increased continuously, implying that application of the bacteriocin to the rice bran had positive effects on prolonging the shelf-life of not only film itself but also the foods wrapped by this film.

COMBUSTION CHARACTERISTICS OF ESTERIFIED RICE BRAN OIL AS AN ALTERNATIVE FUEL IN A DIESEL ENGINE

  • Choi, S.H.;Oh, Y.T.
    • International Journal of Automotive Technology
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    • v.7 no.4
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    • pp.399-406
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    • 2006
  • The smoke emission of diesel engines is being recognized as one of the major source of the air pollution problems. This study investigates the potential of esterified rice bran oil to reduce smoke emission as an alternative fuel for diesel engines. Because the esterified rice bran oil has approximately a 10.5% oxygen content, the combustion of the diesel engine improved and exhaust smoke decreased. Gas chromatography was used to analyze not only the total amount of hydrocarbon but also the amount of hydrocarbon components from $C_1$ to $C_6$ in the exhaust gas to determine an exact source responsible for the remarkable reduction in the smoke emission. The number of individual hydrocarbon($C_1{\sim}C_6$) as well as the total amount of hydrocarbon of esterified rice bran oil reduced significantly compared to that of hydrocarbon of diesel fuel.

Arabinoxylan Rice Bran and Endurance Exercise Training on the TLR4 Signaling-mediated Protein Expression in LPS-treated Rats (유색미 겨 아라비녹실레인과 지구성 운동트레이닝이 LPS 처치된 흰쥐의 TLR4 Signaling 단백질 발현에 미치는 영향)

  • Son, Hee-Jeong
    • The Korean Journal of Food And Nutrition
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    • v.27 no.6
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    • pp.1141-1146
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    • 2014
  • The purpose of this study is to investigate the effects of arabinoxylan rice bran and endurance exercise training on TLR4 mediated protein expression in LPS-treated rats. The results showed that TLR4 as an important protein in the inflammatory response against lipopolysaccharide was shown to be significantly lower in both arabinoxylan supplement with exercise group and exercise group, thus the arabinoxylan rice bran had a higher inhibitory activity than arabinoxylan supplement group. However, $NF-{\kappa}B$ and MyD88 protein expression was not changed in arabinoxylan supplement with exercise training group, whereas $NF-{\kappa}B$ significantly decreased in 4 weeks of exercise training group. These results suggest that the supplement of arabinoxylan rice bran with exercise is likely to contribute to inflammation response and the arabinoxylan rice bran can be used as a possible safe alternative to the immunotherapeutic intervention.

Purification and Biochemical Analysis of Rice Bran Lipase Enzyme (쌀겨로부터 lipase 효소의 정제 및 생화학적인 분석)

  • Kim Younghee
    • Proceedings of the KAIS Fall Conference
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    • 2004.11a
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    • pp.299-301
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    • 2004
  • A simple procedure for the extraction of the lipolytic enzyme from rice bran has been developed. High activity of lipolytic enzyme was obtained by first defatting the rice bran to remove lipid components with various extraction conditions. Then, after five cycles of aqueous extraction, rice bran lipolytic enzyme was purified using micro- and ultrafiltration apparatus. Lipolytic enzyme activity was estimated by its hydrolytic action of tributyrin. The result indicated that the standard activity curve of butyric acid showed that the potential rice bran enzyme is a hydrolytic lipase enzyme. In addition, it showed higher lipolytic activity and specific enzyme activity with further purification by micro- and ultrafiltration.

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Effect of Egg Albumen, Vegetable Oil, Corn Bran, and Cooking Methods on Quality Characteristics of Chicken Nuggets Using Response Surface Methodology

  • Pathera, Ashok Kumar;Riar, Charanjit Singh;Yadav, Sanjay;Singh, Pradeep Kumar
    • Food Science of Animal Resources
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    • v.38 no.5
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    • pp.901-911
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    • 2018
  • Response surface methodology was used to study the effect of egg albumen (5-15 g), vegetable oil (5-15 g), and corn bran (5-15 g) on sensory and textural (firmness and toughness) quality of chicken nuggets cooked by the oven, steam, and microwave methods. The egg albumen and vegetable oil had a positive linear effect but corn bran had a negative linear effect at p<0.01 on sensory overall acceptability scores of nuggets. Firmness and toughness scores were increased significantly (p<0.01) with the increase in corn bran level in the formulation. The optimum level of egg albumen, vegetable oil, and corn bran were obtained and validated. Cooking methods also affected the sensory and textural quality of nuggets. Steam cooked nuggets had higher values of sensory scores than oven and microwave cooked nuggets. Oven cooked nuggets showed higher values of firmness and toughness than steam and microwave cooked nuggets. Results of this study suggest that emulsion based meat products can be enriched with dietary fiber source like corn bran without compromising the sensory and textural quality of the products.

Blending Effect of Palm Oil on Physicochemical Properties of Rice Bran Oil

  • Yoon, Suk-Hoo;Kim, Sun-Ki;Teah, Yau-Kun;Kim, Kil-Hwan;Kwon, Tai-Wan
    • Korean Journal of Food Science and Technology
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    • v.18 no.5
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    • pp.329-333
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    • 1986
  • Rice bran ell was blended with double fractionated palm olein (DF palm olein) to examine the cooking performance of blended oil. A blended oil made with 80% or higher rice bran oil and 20% or less DF palm olein passed the cold test, and had a cloud point of $-3^{\circ}C$. Blending of DF palm olein to rice bran oil lowered the smoke point, refractive index, and absorbancies at 232 and 268 nm of rice bran oil. Dielectric constant of oils was not affected by blending during heating. Blending of DF palm olein , however, increased the acids formation in rice bran oil, whereas it retarded polymer formation. The results of the analytical methods used in this study except dielectric constant measurement showed significant difference among the blended oils depending on the blending ratios.

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Xylanase Production by Bacillus sp. A-6 Isolated from Rice Bran

  • Lee, Jun-Ho;Choi, Suk-Ho
    • Journal of Microbiology and Biotechnology
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    • v.16 no.12
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    • pp.1856-1861
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    • 2006
  • A Bacillus sp. A-6 strain that produced xylanase was isolated from rice bran. The optimal temperature and pH for xylanase activity of the culture supernatant of Bacillus sp. A-6 were 40$^{\circ}C$ and pH 7, respectively. The optimal temperature and pH for xylanase production in the xylan medium were 30$^{\circ}C$ and pH 9, respectively. The optimal concentrations of oat spelt xylan and peptone for xylanase production were 0.5% and 1.5%, respectively. The best nitrogen sources for xylanase production was beef extract, but xylanase production was also supported comparably by tryptone and peptone. The bacterial growth in the optimal xylan medium reached stationary growth phase after 12 h of incubation. The xylanase production in the culture supernatant increased dramatically during the initial 12 h exponential growth phase and then remained constant at 23.8-24.5 unit/ml during the stationary growth phase. The pH of the culture medium decreased from 8.8 to 6.7 during the exponential growth phase and subsequently increased to 8.1 during the stationary growth phase. Rice bran, sorghum bran, and wheat bran as well as oat spelt xylan induced xylanase production. The xylanase production was repressed when glucose was added to the xylan-containing medium.

Effects of Wheat Bran Addition on the Quality of Rye Silage (호밀 사일리지에 있어서 밀기울 첨가 효과)

  • 장진호;한성윤;김대진
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.15 no.4
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    • pp.285-290
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    • 1995
  • This experiment was conducted to investigate the effects of wheat bran addition on the fermentative quality and dry matter digestibility(Dh4D) of rye silage. This hexbage was ensiled by the conventional methods in small glass silo of 0.82 liter with addition of wheat bran of 0%, 5%. 01%, and 15%. mpectivery.The samples of rye silage were determined the pH, lactic acid, acetic acid and butyric acid. The DMD was evaluated by pepsin-cellulase technique method. The energy values (total digestible nutrients. TDN : digestible energy, DE : metabolizable energy, ME) were calculated by DMD. The results obtained were as follows : 1. The pH and butyric acid of rye silage were reduced with increasing of wheat bran addition, but the contents of lactic acid and total acid were increased. 2. TDN, DE and ME were increased with high rate of wheat bran addition. 3. Regression equation and correlation coefficients between Flieg's score(X) and DMD(Y) of silage were Y =48.7+0.157X(r=0.917, p<0.01). 4. Regression equation and correlation coefficients between level of wheat bran(X) and Flieg's score(Y) of silage were Y= 18.3+5.56X(r=0.929, p<0.01).

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Purple Rice Bran Extract Attenuates the Aflatoxin B1-Induced Initiation Stage of Hepatocarcinogenesis by Alteration of Xenobiotic Metabolizing Enzymes

  • Suwannakul, Nattawan;Punvittayagul, Charatda;Jarukamjorn, Kanokwan;Wongpoomchai, Rawiwan
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.8
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    • pp.3371-3376
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    • 2015
  • Pigmented rice bran has been suggested to be a valuable source of beneficial phytochemicals. We investigated genotoxic and anti-genotoxic effects of purple rice bran extract (PRBE) in rats using a liver micronucleus assay. Purple rice bran was extracted with methanol, obtaining large amounts of phenolic compounds, including anthocyanins and small amounts of gamma-oryzanol. The experimental protocols were divided into two sets. Male rats were divided into three groups. Group 1 was a negative control, while Groups 2 and 3 were fed with 100 and 500 mg/kg bw of PRBE, respectively, for 28 days. PRBE had no effect on micronucleus formation or xenobiotic metabolizing enzymes in rat liver. Experiments concerning the effect of PRBE on $AFB_1$ showed that PRBE significantly lessened the amount of micronucleated hepatocytes in $AFB_1$ treated rats. Furthermore, it modulated metabolic activation of $AFB_1$ metabolism in the liver by suppressing activity and protein expression of CYP1A2, CYP3A and CYP 450 reductase, and enhancing phase II enzymes including GST and UGT. Overall, purple rice bran extract was not genotoxic in rats. It exhibited anti-genotoxicity by modulation some xenobiotic enzymes active in $AFB_1$ metabolism.