• Journal of Animal Science and Technology
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• v.52 no.3
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• pp.213-220
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• 2010

This study was executed to investigate the effects of drinking deep sea water treated by reverse osmosis process (RO-DSW) on growth performance, nutrient utilizability, relative weight of lymphoid organs and the concentration of serum immunoglobulin G (IgG) in broiler chickens. A total of 200 one day old broiler chickens (Ross 308) were equally and randomly distributed into 10 ground floor pens (20 chicks per pen, 5 pens per treatment) bedded with rice-husks. The broilers were offered either fresh tap water (Control) or RO-DSW for 28 days (from d 6 to d 33) as the drinking water. The same basal phase 1 diet for first 2 weeks and phase 2 diet for last 2 weeks were offered ad libitum to the birds. The RO-DSW was prepared by diluting 1:20 ratio with deionized water before offering to chickens. The diet for control birds was supplemented with 0.21 % of food-grade salt to satisfy salt need of the birds. Broiler feeding study resulted that there were no differences in amount of water consumption, mortality and FCR between RO-DSW and control chickens. However, feed intake and body weight gain were increased (p<0.05) by RO-DSW drinking. There was no (p>0.05) difference in nutrients utilizability between RO-DSW and fresh water drinking. There were no (p>0.05) differences in the immune response between the control and treatment group. The serum IgG levels were 3.01 vs 2.87 mg/ml and the relative weights of spleen, thymus and bursa of Fabricius were 0.23, 0.18 and 0.20 vs. 0.20, 0.17 and 0.14 for RO-DSW vs. control birds, respectively. The immune responses were tended to be improved by RO-DSW drinking. This study showed an improvement in weight gain and feed intake that could be induced by RO-DSW drinking, although it is difficult to explain the reasons of the improvement at this moment. This study implied that RO-DSW could be successfully used as drinking water to broiler chickens.

• Journal of Animal Science and Technology
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• v.51 no.4
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• pp.307-314
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• 2009

This experiment was conducted to investigate the effects of dietary garlic powder (GP) on growth performance and mRNA expression of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) in broiler chickens. Day-old male chicks (Ross, n=270) were fed diets containing 0% [positive control (PC) with antibiotics or negative control (NC) without antibiotics], 1%, 3% and 5% GP for 6 wks. There were no significant differences in feed intake among the treatments throughout the experimental period. Body weight gains in groups fed dietary GP at 3% and 5% were significantly higher than that of NC group (P<0.05). Feeding GP up to 5% did not exert any adverse effect on weight gain and feed intake. There were no significant differences in the relative weights of liver, spleen, cecum and breast muscle. The content of meat cholesterol in GP containing dietary groups tended to be reduced as compared to NC group. Average infectious bronchitis antibody titers in chicks fed GP containing diets were significantly higher than that of NC group (P<0.05). The mRNA expression of hepatic HMG-CoA reductase was reduced by dietary GP. These results indicate that dietary GP has growth promoting effects and tended to alter cholesterol metabolism in broiler chickens.

• Applied Microscopy
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• v.38 no.4
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• pp.353-361
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• 2008

We aimed to investigate whether the extracts of Lycii fructus, Lycii folium, and estradiol can attenuate on the level of cholesterol on serum and ultrastructure alteration of hepatocytes isolated from ovariectomized rats. Experimental groups are divided into five: Sham (sham-operated), OVX (ovariectomized animal), LCF (OVX, Lycii fructus), LCL (OVX, Lycii folium), and $E_2$ (OVX, estradiol). The body weights of OVX group showed increase compared with Sham, LCF, and LCL groups. The levels of total cholesterol and low-density lipoprotein (LDL) cholesterol were significantly decreased in the LCF and LCL groups compared with those of OVX group. Moreover, the High-density lipoprotein (HDL) cholesterol level was increased in the LCL group. As the results of electron microscopical observation, lipid droplets in the OVX group showed increase compared with Sham group. But in the LCF group not showed lipid droplets. In conclusion, administration of Lycii fructus or Lycii folium lowers the serum cholesterol content of the ovariectomized rats and also the damage of the hepatocytes and formation of lipid droplets.

• Journal of Food Hygiene and Safety
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• v.33 no.5
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• pp.389-398
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• 2018

The purpose of this study was to evaluate anti-obesity activity of Cirsium setidens Nakai test material (CNTM) in 3T3-L1 adipocytes and obese C57BL/6J mice fed with a high-fat diet using various obesity-related in vitro experiments. During adipocyte differentiation, CNTM significantly inhibited lipid accumulation and ROS production compared to controls. To evaluate whether CNTM could exert glycerol release effects on mature 3T3-L1 adipocytes, we treated cells with various concentrations of CNTM for 1 h. Treatment of mature adipocytes with $160-320{\mu}g/mL$ of CNTM increased the release of glycerol, but not in a significant dose-dependent manner. Anti-adipogenic and anti-lipogenic effects of CNTM seemed to be mediated by the inhibition of $PPAR{\gamma}$ and $C/EBP{\alpha}$. Moreover, CNTM stimulated fatty acid oxidation in an AMPK-dependent manner. CNTM-treated groups of C57BL/6J mice showed reduced body weights and adipose tissue weight with improving serum lipid profiles and adiponectin protein expression in obese C57BL/6J mice fed with a high-fat diet. These results suggest that CNTM might have anti-obesity effect on adipogenesis and lipid metabolism in vitro and in vivo. This presents the possibility of developing a treatment for obesity using nontoxic natural resources.

• Food Science of Animal Resources
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• v.26 no.2
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• pp.212-217
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• 2006

Effects of feeding high quality roughage (timothy hay) in growing period on growth performance and carcass characteristics during 24 month (5 month from 28 month to) in twenty of Hanwoo steers were investigated. The hanwoo steers were randomly allotted to 2 treatments (10 heads/treatment) ; rice straw group (control) and timothy hay group (treatment). The final body weights of rice straw group and timothy hay group on 28 month were 667.70 kg and 681.75kg, respectively. The daily gain of total period was slightly higher in timothy hay group (0.83kg ) than in rice straw group (0.81 kg). The daily roughage intake in the growing period was tended to be higher in timothy hay group (4.27kg ) than rice straw group (3.80 kg). The carcass grades (yield grade and quality grade) and Longissimus dorsi area were significantly higher in timothy hay group ($85.62 cm^2$, 2.02 and 2.20 point) than in rice straw group ($81.29 cm^2$, 2.00 and 1.60 point respectively) (p<0.05), and were affected by the feeding with high quality roughage in the growing period.

• The Journal of Korean Medicine
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• v.33 no.3
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• pp.184-199
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• 2012

Objectives: There is a steady increase in the prevalence of obesity worldwide and obesity is often accompanied by inflammation. Although much emphasis has been placed on the adipose tissue inflammation in obesity, a study with herbal medicine is few. This study aimed to investigate the antidiabetic and anti-inflammatory effect of a complex herbal medicine (CHM) composed of Cornus officinalis, Dioscorea rhizoma, Aurantii fructus, and Mori Foliumon on obese type 2 diabetes mice. Methods: Type 2 diabetes mellitus and obesity were induced by Surwit's high fat, high sucrose diet for 8 weeks. Mice were divided into ND (normal diet, n=10), HFD (high fat and high sucrose diet, n=10), CHM (high fat and high sucrose diet with complex herbal medicine, n=10) and Met (high fat and high sucrose diet with metformin, n=10) groups. The body weight, fructosamine and OGTT (oral glucose tolerance test) were measured. After 8 weeks the blood samples of all mice were taken from the heart, and lipid profiles were measured. Epididymal fat pad, histological size of the adipocyte tissue and liver weights were measured. Inflammatory markers such as leptin and adipocyte tissue macrophage were measured to evaluate the effect of CHM on adipocyte tissue inflammation. Results: Compared with the HFD group, there was an improvement in OGTT and epididymal fat decreased in the CHM group. White adipocyte size and adipocyte tissue macrophage decreased in CHM group. Conclusions: These results suggest that CHM has antidiabetic and anti-inflammatory effects in high fat, high sucrose diet induced obese mice.

• Korean Journal of Food Science and Technology
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• v.31 no.2
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• pp.495-501
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• 1999

Lactobacillus acidophilus KY 2104 was isolated from 41 healthy korean ($2{\sim}35\;age$, 26 man, 15 woman) feces to use as a probiotic lactic acid bacteria for functional foods, fermented milk and pharmaceutical products. L. acidophilus KY 2104 survived in the presence of acid and bile, and inhibited pathogens. It survived almost 100% in pH 3.0 phosphate buffer for 2 hr, and more than $10^4\;cfu/mL$ in pH 2.0 phosphate buffer when inoculated by $10^7\;cfu/mL$. It was also found to grow in MRS medium containing 0.5% oxgall. L. acidophilus KY 2104 exerted inhibitory effect against enteric pathogens such as Escherichia coli, Shigella dysenteriae, Staphylococcus aureus and Salmonella typhimurium, when grown with each in associative cultures for 16 hr. As a result of acute toxicity of L. acidophilus KY 2104 in rats, there was no observation on motality, clinical signs, body weights and gross findings in the oral administration.

• Korean Journal of Food Science and Technology
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• v.35 no.3
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• pp.519-526
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• 2003

Pancreatic lipase and ${\alpha}$-amylase inhibitory activities in purified extracts of pumpkin and job's tear were studied. Pancreatic lipase inhibitory activity was determined by measuring the rate of releasing oleic acid from triolein, and ${\alpha}$-amylase inhibitory activity was determined by iodometric method. The extracts of pumpkin and job's tear were purified using silica gel and C-18 gel column chromatographies. Treatment of pumpkin extract $(120\;{\mu}g/mL)$ in 3T3-L1 preadipocyte decreased differentiation about 95% and blocked accumulation of lipid. Body weights of rats fed high-fat diet containing dietary supplement decreased about 13% as compared with those fed only highfat diet. These results revealed dietary supplement is a good obesity-reducing material for decreasing lipid and carbohydrate absorptions.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.8
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• pp.1265-1272
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• 2020

Objective: To evaluate the effect of feeding acidified milk on the growth and fecal microbial diversity of dairy calves. Methods: Twenty healthy 3-day-old female Holstein calves with similar body weights were selected and randomly divided into two groups. One group was fed pasteurized milk (PM, Control), while the other was fed acidified milk (AM) ad libitum until weaned (day 60). The experiment lasted until day 180. Results: There was no difference in the nutritional components between PM and AM. The numbers of Escherichia coli and total bacteria in AM were lower than in PM. At 31 to 40 and 41 to 50 days of age, the milk intake of calves fed AM was higher than that of calves fed PM (p<0.05), and the solid feed intake of calves fed AM was higher than that of calves fed PM at 61 to 90 days (p<0.05). The average daily gain of calves fed AM was also higher than that of calves fed PM at 31 to 60, 61 to 180, and 7 to 180 days (p<0.05). The calves fed AM tended to have a lower diarrhea rate than those fed PM (p = 0.059). Bacteroides had the highest abundance in the feces of calves fed AM on day 50, while Ruminococcaceae_UCG_005 had the highest abundance in the feces of calves fed AM on day 90 and calves fed PM on days 50 and 90. At the taxonomic level, the linear discriminant analysis scores of 27 microorganisms in the feces of calves fed AM and PM on days 50 and 90 were higher than 4.0. Conclusion: Feeding AM increased calf average daily gain and affected fecal bacterial diversity.

• Food Science and Preservation
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• v.21 no.1
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• pp.107-113
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• 2014

The present study was designed to investigate the antihyperlipidemic effect of Coconopsis lanceolata extract in C57BL/6J mice. The mice were divided into four groups: normal diet group (ND), high fat diet group (HFD), positive control group with 0.05% metformin (PC), Coconopsis lanceolata extract group (UCL). After 5 weeks of feeding, average body weight of the UCL group mice was slightly decreased, while that of the HFD group significantly increased) Also, liver and adipose tissue weights in the UCL group significantly increased. The levels of trigliceride (TG) and total in the plasma of UCL-supplemented group were significantly lower than those of high fat diet group. On the other hand, HDL-cholesterol level was increased. Expression level of proteins related with adipogenesis such as SREBP-1c, ACC, and FAS in the liver of the UCL group mice was much lower comparing with the HFD group mice. In conclusion, the results showed that the Coconopsis lanceolata extract possesses significant antihyperlipidemic effects in C57BL/6J mice.