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http://dx.doi.org/10.13103/JFHS.2018.33.5.389

Anti-obesity Activities of Cirsium setidens Nakai Ethanolic Extract  

Cho, Bong-Yeon (Department of Food Science and Biotechnology, Kangwon National University)
Choi, Sun-Il (Department of Food Science and Biotechnology, Kangwon National University)
Choi, Seung-Hyun (Department of Food Science and Biotechnology, Kangwon National University)
Sim, Wan-Sup (Department of Food Science and Biotechnology, Kangwon National University)
Xionggao, Han (Department of Food Science and Biotechnology, Kangwon National University)
Ra, Moon-Jin (Hongcheon Institute of Medicinal Herb)
Kim, Sun-Young (Hongcheon Institute of Medicinal Herb)
Kang, Il-Jun (Department of Food Science and Nutrition, Hallym University)
Han, Kyoung-Chan (HATTI Co., Ltd.)
Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
Publication Information
Journal of Food Hygiene and Safety / v.33, no.5, 2018 , pp. 389-398 More about this Journal
Abstract
The purpose of this study was to evaluate anti-obesity activity of Cirsium setidens Nakai test material (CNTM) in 3T3-L1 adipocytes and obese C57BL/6J mice fed with a high-fat diet using various obesity-related in vitro experiments. During adipocyte differentiation, CNTM significantly inhibited lipid accumulation and ROS production compared to controls. To evaluate whether CNTM could exert glycerol release effects on mature 3T3-L1 adipocytes, we treated cells with various concentrations of CNTM for 1 h. Treatment of mature adipocytes with $160-320{\mu}g/mL$ of CNTM increased the release of glycerol, but not in a significant dose-dependent manner. Anti-adipogenic and anti-lipogenic effects of CNTM seemed to be mediated by the inhibition of $PPAR{\gamma}$ and $C/EBP{\alpha}$. Moreover, CNTM stimulated fatty acid oxidation in an AMPK-dependent manner. CNTM-treated groups of C57BL/6J mice showed reduced body weights and adipose tissue weight with improving serum lipid profiles and adiponectin protein expression in obese C57BL/6J mice fed with a high-fat diet. These results suggest that CNTM might have anti-obesity effect on adipogenesis and lipid metabolism in vitro and in vivo. This presents the possibility of developing a treatment for obesity using nontoxic natural resources.
Keywords
Antiobesity; C. setidens Nakai ethanolic extract; 3T3-L1; C57BL/6J mice;
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