Journal of Animal Science and Technology

  • 제51권4호
  • /
  • Pages.307-314
  • /
  • 2009
  • /
  • 2672-0191(pISSN)
  • /
  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
권호 표지
DOI QR코드

DOI QR Code

육계 사료 내 마늘분말의 첨가급여가 육계 성장과 HMG-CoA Reductase의 mRNA 발현에 미치는 영향

Effects of Dietary Garlic Powder on Growth Performance and mRNA Expression of Hepatic HMG-CoA Reductase in Broiler Chickens

  • 유선종 (건국대학교 동물생명과학대학 동물자원연구센터) ;
  • 안병기 (건국대학교 동물생명과학대학 동물자원연구센터) ;
  • 강창원 (건국대학교 동물생명과학대학 동물자원연구센터)
  • You, Sun-Jong (Animal Resources Research Center, College of Animal Bioscience and Technology, Konkuk University) ;
  • Ahn, Byoung-Ki (Animal Resources Research Center, College of Animal Bioscience and Technology, Konkuk University) ;
  • Kang, Chang-Won (Animal Resources Research Center, College of Animal Bioscience and Technology, Konkuk University)
  • 투고 : 2009.02.20
  • 심사 : 2009.07.14
  • 발행 : 2009.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

육계사료 내 마늘분말의 첨가급여가 육계의 성장성적과 간 HMG-CoA reductase의 발현에 미치는 영향을 규명하기 위해 본 연구를 수행하였다. 1일령의 육계 Ross 수평아리에게 마늘분말을 0%(항생제 첨가 또는 항생제 무첨가), 1%, 3% 및 5%의 수준으로 첨가한 실험사료를 각각 6주간 급여하였다. 증체량은 항생제 무첨가 대조구에 비하여 마늘분말 3% 및 5%를 첨가한 처리구에서 유의하게 증가하였으며(P<0.05), 사료섭취량은 처리구간 차이가 없었다. 육계 사료 내 5%까지의 마늘분말의 첨가급여는 증체량과 사료섭취량에 부정적인 영향을 주지 않았다. 간, 비장, 맹장 및 가슴 근육에 대한 상대적 중량은 처리가 유의한 차이가 관찰되지 않았다. 근육 내 콜레스레롤 함량은 항생제 첨가 대조구에 비해 감소하였으나 통계적인 유의차는 나타나지 않았다. IB 항체 생성량은 항생제 무첨가 대조구에 비하여 유의적으로 증가하였다(P<0.05). 간의 HMGCoA reductase mRNA의 발현은 대조구에 비해 마늘분말을 첨가한 모든 처리구에서 저하된 것으로 관찰되었다. 본 연구에서 사료 내 마늘의 첨가급여는 육계의 성장촉진 효과를 나타내었으며, 콜레스테롤 대사에 영향을 미칠 수 있음이 시사되었다.

This experiment was conducted to investigate the effects of dietary garlic powder (GP) on growth performance and mRNA expression of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) in broiler chickens. Day-old male chicks (Ross, n=270) were fed diets containing 0% [positive control (PC) with antibiotics or negative control (NC) without antibiotics], 1%, 3% and 5% GP for 6 wks. There were no significant differences in feed intake among the treatments throughout the experimental period. Body weight gains in groups fed dietary GP at 3% and 5% were significantly higher than that of NC group (P<0.05). Feeding GP up to 5% did not exert any adverse effect on weight gain and feed intake. There were no significant differences in the relative weights of liver, spleen, cecum and breast muscle. The content of meat cholesterol in GP containing dietary groups tended to be reduced as compared to NC group. Average infectious bronchitis antibody titers in chicks fed GP containing diets were significantly higher than that of NC group (P<0.05). The mRNA expression of hepatic HMG-CoA reductase was reduced by dietary GP. These results indicate that dietary GP has growth promoting effects and tended to alter cholesterol metabolism in broiler chickens.

키워드

참고문헌

  1. Adams, M. L., Sullivan, D. M., Smith, R. L. and Richter, E. F. 1986. Evaluation of direct saponification method for determination of cholesterol in meats. J. Assoc. Off. Anal. Chem. 69(5):844-846.
  2. Bast, A., Chandler, R. F., Choy, P. C., Luc, L. M., Delulle, M., Gruenwald, J., Bart, S., Halkes, A., Keller, K., Koeman, J. H., Peters, P., Przyrembel, H., de Ree, E. M., Renwick, A. G. and Vermeer, I. T. M. 2002. Botanical health products; positioning and requirements for effective and safe use. Environ. Toxicol. & Pharm. 12:195-211. https://doi.org/10.1016/S1382-6689(02)00035-2
  3. Campbell, J. H., Efendy, J. L., Smith, N. J. and Campbell, G. R. 2001. Molercular basis by which garlic suppresses atherosclerosis. J. Nutr. 131:1006-1009.
  4. Cheng, M. H. and Jung, T. C. 1981. Effect of allithiamine on sacroma-180 tumor growth in mice. Tai-wan I Husuch Hui Tsa chif 80(4):385-393.
  5. Chomczylnski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
  6. Chowdhury, S. R., Chowdhury, S. D. and Smith, T. K. 2002. Effects of dietary garlic on cholesterol metabolism in laying hens. Poultry Sci. 81:1856-1862.
  7. Duncan, D. B. 1955. Multiple range and multiple F test. Biometr. 11:1-42. https://doi.org/10.2307/3001478
  8. Elkin, R. G., Freed, M. B., Kieft, K. A. and Newton, R. S. 1993. Alteration of egg yolk cholesterol content and plasma lipoprotein profiles following administration of a totally synthetic HMG-CoA reductase inhibitor to laying hens. J. Agric. Food Chem. 41:1094-1101. https://doi.org/10.1021/jf00031a016
  9. Elkin, R. G., Zhong, Z. Y., Donkin, S. S., Buhman, K. K., Story, J. A., Turek, J. J., Porter Jr, R. E., Anderson, M., Homan, R. and Newton, R. S. 1999. Select 3-Hydroxy-3methylglytaryl-coenzyme A reductase inhibitors vary in their ability to reduce egg yolk cholesterol levels in laying hens through alteration of hepatic cholesterol biosynthesis and plasma VLDL composition. J. Nutr. 129:1010-1019.
  10. Fleischauer, A. T. and Arab, L. 2001. Garlic and cancer: A critical review of the epidemiologic literature. J. Nutr. 131: 1032-1040.
  11. Folch, G., Lees, M. and Stanley, G. H. S. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
  12. Gebhardt, R. 1993. Multiple inhibitory effects of garlic extracts on cholesterol biosynthesis in hepatocytes. Lipids 24(7):613-619.
  13. Gebhardt, R. and Beck, H. 1996. Differential inhibitory effects of garlic derived organosulfur compound on cholesterol biosynthesis in primary rat hepatocyte cultures. Lipids 31(12): 1269-1276. https://doi.org/10.1007/BF02587912
  14. Konjufca, V. H., Pesti, G. M. and Bakalli, R. I. 1997. Modulation of cholesterol levels in broiler meat by dietary garlic and copper. Poultry Sci. 76:1264-1271.
  15. Kyo, E., Uda, N., Kasuga, S. and Itakura, Y. 2001. Immunomodulatory effects of aged garlic extract. J. Nutr 131: 1075-1079.
  16. Lamm, D. L. and Riggs, D. R. 2001. Enhanced immunocompetence by garlic: Role in bladder cancer and other malignancies. J. Nutr. 131:1067-1070.
  17. Lau, B. H. S. 2001. Suppression of LDL oxidation by garlic. J. Nutr. 131:985-988.
  18. Liu, L. and Yeh, Y. Y. 2000. Inhibition of cholesterol biosynthesis by organosulfur compounds derived from garlic. Lipids 35(2):197-203. https://doi.org/10.1007/BF02664770
  19. Milner, J. A. 2001. A historical perspective on garlic and cancer. J. Nutr. 131:1027-1031.
  20. Qureshi, A. A., Abuirmeileh, N., Din, Z. Z., Elson, C. and Burger, W. C. 1983a. Inhibition of cholesterol and fatty acid biosynthesis in liver enzymes and chicken hepatocytes by polar fraction of garlic. Lipids 18:343-348. https://doi.org/10.1007/BF02537229
  21. Qureshi, A. A., Din, Z. Z., Abuirmeileh, N., Burger, W. C., Ahmad, Y. and Elson, C. E. 1983b. Suppression of avian hepatic lipid metabolism by solvent extract of garlic: Impact on serum lipids. J. Nutr. 113:1746-1755.
  22. Rahman, K. 2001. Historical perspective on garlic and cardiovascular disease. J. Nutr. 131:977-979.
  23. SAS Institute Inc. 1989. SAS/STAT User's Guide. Version 6, 4th Edition. SAS Institute Inc., Cary, NC.
  24. Schaefer, E. J. 2002. Lipoproteins, nutrition, and heart disease. Am. J. Clin. Nutr. 75:191-212.
  25. Sharma, K. K. and Sharma, S. P. 1979. Effect of onion and garlic on serum cholesterol on normal subjects. Mediscope. 22(7):134-136.
  26. Slowing K., Gando, P., Sanz, M., Ruiz, E. and Tejerina, T. 2001. Study of garlic extracts and fractions on cholesterol plasma levels and vascular reactivity in cholesterol-fed rats. J. Nutr. 131:994-999.
  27. Tompson, R. H. and Merola, G. V. 1993. A simplified alternative to the AOAC official method for cholesterol in multicomponent foods. J. AOAC inter. 76(5):1057-1068.
  28. Yeh, Y. Y. and Liu, L. 2001. Cholesterol-lowering effect of garlic extracts and organosulfur compounds: Human and animal studies. J. Nutr. 131:989-993.
  29. 문영자, 염금희, 성창근. 2002. HMG-CoA reductase 저해제 탐색과 가금의 콜레스테롤 저하 효과. 한국식품영양학회. 15 (4):307-313.
  30. 윤병선, 남기택, 김창원, 강창원, S. Ohtani, K, Tanaka. 1996. 육계 사료 내 마늘의 첨가가 육계의 생산성과 HMG-CoA reductase에 미치는 영향. 한국가금학회지. 23(3):129-134.
  31. 윤병선, 채현석, 김석철, 김동운, 안종남, 김용곤. 1998. 산란 계에 대한 마늘의 급여 효과. 한국영양사료학회지. 22(6):357-362.
  32. 최용순, 이상영. 1992. 혈청콜레스테롤과 3-hydroxy-3-methylglutaryl coenzyme a reductase. 한국영양식량학회지. 21(5): 580-593.

피인용 문헌

  1. Garlic powder intake and cardiovascular risk factors: a meta-analysis of randomized controlled clinical trials vol.8, pp.6, 2014, https://doi.org/10.4162/nrp.2014.8.6.644
  2. Effect of Dietary Fermented Garlic Solution on Performance, Egg Quality and Blood Composition in Finishing Period of Laying Hens vol.45, pp.3, 2018, https://doi.org/10.5536/KJPS.2018.45.3.201