• 생명과학회지
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• 제25권11호
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• pp.1255-1264
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• 2015

천궁(C. officinale)은 예로부터 민간처방 약재로 사용되었으며, 항염증, 항산화, 항암 및 신생혈관억제 등의 효능을 가지는 것으로 알려져 있다. 하지만 혈구암세포에서 apoptosis 유발과 관련된 분자생물학적 기전에 대해서는 명확히 밝혀져 있지 않다. 본 연구에서는 인체 혈구암세포인 U937 세포에서 천궁의 열수, 에탄올 및 메탄올 추출물(WECO, EECO 및 MECO)이 유발하는 항암효과 및 항암기전을 조사하였다. 먼저 WECO, EECO 및 MECO가 유발하는 증식억제 정도를 조사한 결과 EECO가 가장 뛰어난 효능을 가진다는 것을 알 수 있었으며, 이러한 현상이 apoptosis 유발에 의한 것임을 annexin-V 염색, apoptotic body 형성, DNA 단편화 및 MMP 소실 등을 통하여 확인하였다. EECO 처리에 의한 apoptosis 유발에는 DR4의 발현 증가와 함께 cIAP-1, Bcl-2 및 total Bid의 발현감소가 관여하였으며, caspases-3, -8 및 -9의 활성화와 함께 caspases-3의 기질 단백질인 PARP, β-catenin 및 PLC γ1의 단편화도 관찰되었다. 또한 EECO는 AMPK signaling pathway를 활성화시키는 것으로 나타났으며, AMPK 억제제인 compound C를 이용하여 AMPK의 활성을 억제하였을 경우 EECO에 의하여 유발되었던 apoptosis가 현저하게 감소되는 것으로 나타났다. 이상의 결과를 살펴볼 때 인체 혈구암세포인 U937 세포에서 EECO에 의하여 유발되는 apoptosis는 AMPK가 중요한 조절자로서 작용하는 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권12호
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• pp.1909-1916
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• 2007

This study compared the growth performance and carcass evaluation of Jeju native cattle (JNC) and its crossbreds (CBK = 25 JNC:50 Charolais:25 Brahman and BCBK = 62.5 JNC:25 Charolais:12.5 Brahman). Eight male calves of each JNC, CBK and BCBK were weaned at 4 month of age and were fed for 24 months of age. All animals grazed a pasture between 5 to 10 months of age then they were fed growing ration at the rate of 1.5% of their BW along with ad libitum supply of Italian ryegrass hay between 11 to 16 months of age and thereafter switched to ad libitum feeding of finishing ration and hay between 17 to 24 months of age. Mean body weight (BW) and BW gain were higher in CBK compared with BCBK and JNC at 4, 10, 16 and 24 months of age. Average daily BW gain during 4 to 10 months of age was the highest for CBK followed by BCBK and JNC. However, daily BW gain was higher in BCBK than in CBK and JNC during 11 and 16 months of age. During fattening period (17 to 24 months) average daily BW gain was higher in JNC than in CBK and BCBK. Slaughter weight, hot and cold carcass weight were higher in CBK compared with JNC and BCBK. Weight of bones, boneless meat, ribs, excluded rib meat, retailed cut meat, tender loin, sir loin, strip loin, sticking, top round, bottom round, fore leg, shank, and thin-flank were higher in CBK than in BCBK and JNC. Fat weight in these carcass cuts and kidney fat was similar in JNC and its crossbreds. Logissimus dorsi and its ratio were higher in CBK compared with BCBK and JNC. Percent moisture, crude protein, and ash contents of beef were similar in JNC and its crossbreds. Percent beef fat was higher in JNC and BCBK than in CBK. Cooking loss and water holding capacity of beef was similar in JNC and its crossbreds. Sheer force was lower in BCBK compared with JNC and CBK. Juiciness, tenderness and flavor of beef were higher in BCBK compared with JNC and CBK. In conclusion, CBK has shown higher growth rate and produced heavier carcasses with good degree of fatness compared with JNC and BCBK. However, fattening for longer period could increase the maintenance cost in CBK and BCBK because of their higher BW which they attained during growing period.

• 한국축산식품학회지
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• 제30권6호
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• pp.1007-1013
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• 2010

시험동물은 $68.77{\pm}0.10kg$인 3원 교잡종(Landrace${\times}$Yorkshire${\times}$Duroc) 비육돈 96두를 공시하였으며, 8주간 사양시험을 실시하였다. 시험설계는 1) corn-soybean meal diet, 2) corn-soybean meal diet + 0.05% enzyme complex, 3) cornsoybean meal diet with DDGS 및 4) corn-soybean meal diet with DDGS + 0.05% enzyme complex으로 4개 처리를 하여 처리당 6반복, 반복당 4두씩 완전임의 배치하였다. 등심의 pH는 DDGS를 함유한 두 처리구가 CON - Enzyme 처리구와 비교하여 유의적으로 증가하였다(p<0.01). 관능평가에 있어서 경도(Firmness)은 DDGS - Enzyme 처리구가 CON 처리구와 비교하여 유의적으로 증가하였으며(p<0.01), 육색에 있어서 $a^*$ 값은 DDGS를 함유한 두 처리구가 DDGS를 함유하지 않은 두 처리구와 비교하여 유의적으로 높게 나타났다(p<0.05). 등심 및 등지방의 지방산 조성에서 총 UFA와 총 UFA/SFA는 DDGS를 함유한 처리구가 CON 처리구와 비교하여 유의적으로 높은 효과를 나타냈다(p<0.001). 결론적으로 DDGS 급여는 등심의 pH, 경도, 육색, 불포화 지방산 조성을 증가시켰으나, 복합 효소제 첨가 급여에 따른 육질의 변화는 크게 나타나지 않았다.

• 한국초지조사료학회지
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• 제29권4호
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• pp.375-382
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• 2009

본 시험을 수행하기 위해서 6개월령 거세한 우 64두를 공시하여 주구는 조사료원으로 목건초와 볏짚 처리구를 배치하였으며, 세구는 출하시기로 24개월, 26개월, 28개월 및 30개월 배치하였다. 본 시험은 조사료원 및 출하시기별 발육 및 육질에 미치는 영향을 구명하기 위하여 수행하였다. 발육 및 사료이용성은 목건초 급여구가 볏짚 급여구에 비하여 동일 출하월령에서 10~12% 정도 더 발육이 향상되었으며(p<0.05), 배합사료 요구량에서도 목건초 급여 구가 볏짚 급여구에 비하여 kg 증체당이 향상 되는 결과를 얻었다. 또한 도체등급에서도 등 지방두께 및 배최장근단면적은 출하월령이 증가할수록 증가하였으며, 근내지방도 역시 출하월령이 증가할수록 증가하는 경향을 보였다. 따라서 한우 고급육을 생산하기 위해서는 볏짚 보다는 목건초 급여로 출하시기를 28개월령 이상 사육하는 것이 바람직한 것으로 사료된다.

• Journal of Animal Science and Technology
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• 제62권1호
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• pp.43-51
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• 2020

This experiment was conducted to investigate the effects of exogenous emulsifier supplementation on growth performance, energy digestibility, and meat quality in broilers. A total of 60 Ross 308 broilers were treated for two weeks. The three dietary treatments were: (CON) basal diet; (T1) basal diet + 0.1% exogenous emulsifier, and (T2) basal diet + 0.2% exogenous emulsifier. In Period 1 (0-7 days), broilers in the T2 group showed significantly higher body weight gain (BWG) (p < 0.05) and broilers in the T1 and T2 treatment groups had significantly lower feed conversion ratios (FCR) (p < 0.05). In Period 2 (8-14 days), broilers in the T2 treatment group had significantly higher feed intake (FI) (p < 0.05). Therefore, in this experiment (from days 0 to 19), BWG and FCR were affected (p < 0.05) by the T1 and T2 treatments. Additionally, the T1 and T2 treatments with added exogenous emulsifier in the broiler feed showed significantly higher energy digestibility (p < 0.05) than the CON treatment. Broilers fed the T2 diet had higher water-holding capacity (WHC) (p < 0.05) and cooking loss than the broilers fed the CON and T1 diets. Moreover, the shearing force in the meat was decreased (p < 0.05) in broilers fed the T2 diet. In conclusion, supplementation with exogenous emulsifier to broiler diets improved growth performance, energy digestibility, and meat quality. The optimal amount of exogenous emulsifier supplementation requires further investigation.

• 한국식품영양과학회지
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• 제37권6호
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• pp.775-783
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• 2008

본 연구에서는 옐로우 레이어 케이크 제조 시 트랜스지방산 함량을 줄이고 인체 내의 지방 축적을 억제시켜 저열량의 효과가 있는 다이아실글리세롤 오일을 사용하였을 때의 품질 특성을 보고자하였다. 대조구는 마가린만을 사용하였고, 실험처리구는 마가린 무게에 대하여 다이아실글리세롤 오일의 첨가량을 10, 20, 30, 40%로 각각 다르게 첨가하여 옐로우 레이어 케이크를 제조하였다. 반죽의 pH 측정 결과 대조구에 비해 다이아실글리세롤 오일의 첨가량이 증가할수록 pH가 높아지는 경향을 보였다. 반죽의 비중은 대조구보다 다이아실글리세롤 오일의 첨가량이 증가할수록 높게 나타났으며, 반죽의 겉보기 점도는 대조구보다 다이아실글리세롤 오일의 첨가량이 증가할수록 높은 점도를 나타내었다. 케이크의 pH 측정결과는 반죽의 pH와 같은 경향이었으며, 다이아실글리세롤 오일을 30% 첨가 시 부피와 수분함량이 높은 결과를 보였다. 케이크의 명도는 첨가량이 증가할수록 높아졌고, 적색도, 황색도, 총색차는 대조구에 비해 첨가량이 증가할수록 낮게 나타났다. 텍스처의 경우 경도, 부착성, 검성과 씹힘성은 대조구보다 다이아실글리세롤 오일의 첨가량이 증가할수록 낮은 결과를 보였고, 탄력성과 응집성은 커지는 결과를 보였다. 관능검사에서는 다이아실글리세롤 오일을 20%와 30% 첨가 시 높은 점수를 받았다. 그러므로 다이아실글리세롤 오일을 30% 첨가하여 옐로우 레이어 케이크를 만들 경우 맛과 품질을 향상시켜주고, 트랜스지방산의 함량을 줄이며, 인체 내의 지방 축적을 억제시켜 저열량 케이크를 만들 수 있을 것으로 기대된다.

• 한국축산식품학회지
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• 제32권2호
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• pp.133-141
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• 2012

This study was carried out to investigate the effects of substitution of fermented king oyster mushroom (P. eryngii) by-products diet on pork meat quality characteristics, during the storage. A mixture of 40% king oyster mushroom by-products, 28% soybean meal and 20% corn was fermented for 10 d, and the basal diet was then substituted by the fermented diet mixture of up to 20, 50 and 80%, respectively. A total of 96 pigs were fed experimental diet (8 pigs per pen ${\times}$ 4 diets ${\times}$ 3 replication), and eight longissiumus (LD) per treatment were collected, when each swine reached to 110 kg of body weight. The Warner-Bratzler shear forces and cooking loss were significantly lowered in the treatments, while crude protein content and water holding capacity significantly (p<0.05) increased in the treatments than in the control group. The volatile basic nitrogen (VBN), at 1 d of storage, was lower in the treatments, while texture profiles and sensory evaluation did not differ between the control and the treatments (p>0.05). The pH, thiobarbituric acid reactive substances (TBARS), VBN and meat color in all treatments were increased as storage increased. Fermented king oyster mushroom by-products diet effects on lightness (CIE $L^*$), yellowness (CIE $b^*$) and chroma were determined, when LD muscles in T2 and T3 treatments were higher (p<0.05), up to 7 d (p<0.05). Therefore, the results indicate that the substitution of the fermented king oyster mushroom by-products diet to swine diet influenced the quality of the meat and it may be an economically valuable ingredient.

• Journal of Ginseng Research
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• 제11권2호
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• pp.145-252
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• 1987

Isolated rat adipocytes are well known to possess opposite pathways of lipid metabolism: lipolysis and ipogenesis. Both of the metabolism respond to various biologically active substances such as epinephrine, ACTH and insulin. Epinephrine and ACTH stimulate lipolysis and insulin accelerates lipogenesis. Recently, Korean red ginseng powder was found to contain adenosine and an acidic poptide which inhibited epinephrine-induced lipolysis and sl imulated insulin-mediated lipogenesis from added glucose. The acidic peptide is consisted mainly of glutamic acid and glucose. Ginsenosides Rb1 and Re inhibited ACTH-induced lipolysis in isolated rat adipocytes, while they did not affect insulinstimulated lipogenesis, Thus, all these substances extracted from Korean red ginseng exhibited selective modulations toward the opposite metabolic pathways in rat adipocyte; They inhibited the lipolysis but not the lipogenesis. We call these substances"selective modulators". Recently, we isolated a toxic substance named "toxohormone-L " from ascites fluid of patients with various malignant tumors. The toxohormone-L stimulated lipolysis in rat adipocytes and induced anorexia in rats. Both the lipolytic and the anorexigenic actions of toxohormone-L were found to be inhibited by ginsenoside Rb2 in Korean red ginseng. Based on these results, physiological signifi¬cances of these substances in Korean red ginseng were discussed. Pan ax ginseng is a medicinal plant long used in treatment of various pathological states including general complaints such as head ache, shoulder ache, chilly constitution and anorexia in cancer patients, There have been many pharmacological studies on Panax ginseng roots. Petkovllreported that oral administration of an aqueous alcoholic extract of ginseng roots decreased the blood sugar levtl of rabbits. Saito2lreported that Panax ginseng suppressed hyperglycemia induced by epinephrine and high carbohydrate diets. These findings suggest that Panax ginseng roots contain insulin-like substances. Previously, we demonstrated that gin¬seng roots contain an insulin-like peptide which inhibits epinephrine-induced lipolysis and stimulated insulin-mediated lipogenesis. In 1984, we suggested that such an insulin-like substance should be called a selective modulator4). Present investigation describes the details of the selective modulators in ginseng roots. During progressive weight loss in patients with various neoplastic disease, depletion of fat stores have been observed. The depletion of body fat during growth of neoplasms is associated with increase in plasma free fatty acids. Recently, we found that the ascites fluid from patients with hepatoma or ovarian tumor and the pleural fluid from patients with malignant lymphoma elicited fatty acid release in slices of rat adipose tissue in vitro. The lipolytic factor, named"toxohormone-L". was purifed from the ascites fluid of patients with hepatoma. The isolated preparation gave a single band on both disc gel electrophoresis and sodium dodecyl sulfate(SDS)-acrylamide gel electrophoresis in the presence of ${\beta}$-mercaptoethanol. Its molecular weight was determined to be 70,000-75,000 and 65,000 by SDS-acrylamide gel electrophoresis and analytical ultracentrifugation, respectively. Injection of toxohormone-L into the lateral ventricle of rats significantly suppressed food and water intakes. There was at least 5 hr delay between its injection and appearance of its suppressive effect. In the present study, we also tried to find a inhibitory substance toward toxohormone-L from root powder of ginseng.

• Archives of Pharmacal Research
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• 제21권5호
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• pp.581-590
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• 1998

We developed a novel water-soluble camptothecin analobue, CKD602, and evaluated the inhibition of topoisomerase I and the antitumor activities against mammalian tumor cells and human tumor xenografts. CKD602 was a nanomolar inhibitor of the topoisomerase I enzyme in the cleavable complex assay. CKD602 was found to be 3 times and slightly more potent than topotecan and camptothecin as inhibitors of topoisomerase, respecitively. In tumor cell cytotoxicity, CKD602 was more potent than topotecan in 14 out of 26 human cancer cell lines tested, while it was comparable to camptothecin. CKD602 was tested for the in vivo antitumor activity against the human tumor xenograft models. CKD602 was able to imduce regression of established HT-29, WIDR and CX-1 colon tumors, LX-1 lung tumor, MX-1 breast tumor and SKOV-3 ovarian tumor as much as 80, 94, 76, 67, 87% and 88%, respectively, with comparable body weight changes to those of topotecan. Also the therapeutic margin (R/Emax: maximum tolerance dose/$ED-{58}$) of CKD602 was significantly higher than that of topotecan by 4 times. Efficacy was determined at the maximal tolerated dose levels using schedule dependent i.p. administration in mice bearing L1210 leukemia. On a Q4dx4 (every 4 day for 4 doses) schedule, the maximum tolerated dose (MTD) was 25 mg/kg per administration, which caused great weight loss and lethality in <5% tumor bearing mouse. this schedule brought significant increase in life span (ILS), 212%, with 33% of long-term survivals. The ex vivo antitumor activity of CKD602 was compared with that of topotecan and the mean antitumor index (ATI) values recorded for CKD602 were significantly higher than that noted for topotecan. From these results, CKD602 warrants further clinical investigations as a potent inhibitor of topoisomerase I.

• 대한화장품학회지
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• 제41권4호
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• pp.413-420
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• 2015

피부는 신체에서 가장 크고 육중한 기관 중 하나로 인간의 생리 및 병리 과정에 참여한다. 피부는 자기 유지 및 치유, 기계적 및 화학적 손상 방어, 자외선 과 외부 병원성 미생물로부터의 방어, 비타민 D 합성 그리고 사회 심리적 기능을 한다고 알려져 있다. 이 연구의 목적은 한국 여성의 부위와 연령에 따라 피부 생물학 인자와 연관된 생물리학 인자의 변화를 평가하는 데 있다. 20 ~ 49세의 약 70명의 건강한 성인 여성이 이 실험에 참여하였다. 측정부위는 하박 내측과 뺨으로 진행하였다. 인체 피부의 생물리학 인자를 측정하기 위하여 여러 가지 비침습적인 방법으로 진행하였다. 피부의 생물학 인자를 분석하기 위하여 코티졸, 파이브로넥틴, 케라틴-1, 10, 11, 인보루크린, 케라틴 6를 인체의 얼굴과 하박내측으로 비교하였다. 또한 비침습적 방법으로 피부 생물리학 인자는 피부 부위와 연령에 따른 차이를 측정하였다. 측정 부위에 따른 결과, 각질층 수분량, 경피수분손실량과 피부색(L과 a값)은 유의적인 차이가 나타났다. 연령에 따른 결과, 오직 피부색에서만 연령에 따른 차이가 유의적으로 나타났다. 코티졸, 케라틴-6, 파이브로넥틴, 케라틴-1, 10, 11 은 연령과 부위간 유의적 차이가 없지만 인볼루크린은 30 ~ 39세 연령대에서 다른 연령대보다 유의적으로 가장 높았다. 이러한 결과는 개인의 피부 환경에 대한 상세한 피부 상태 변화로 설명할 수 있을 것이다.