• International Journal of Industrial Entomology and Biomaterials
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• v.21 no.2
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• pp.157-162
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• 2010

Open reading frame 35 (bm35) of the Bombyx mori nucleopolyhedrovirus (BmNPV) is a special gene whose homologues are only found in some group-I nucleopolyhedroviruses, suggesting that bm35 plays a specific role in the viral life cycle. This paper described the characterization of BmNPV bm35. Computerassisted sequence analysis shows that a putative RING finger motif is observed in the protein, Bm35 encoded by bm35. The coding sequence of bm35 was amplified and subcloned into the vector pET30a(+) and the $(His)_6$-tagged fusion protein His-Bm35 was expressed in the Escherichia coli BL21 (DE3) LysS cells. The bm35 transcript and Bm35 protein were detected in BmNPV-infected BmN cells at 12~48 h post infection (p.i.) by RT-PCR and Western blot analysis using the polyclonal antibody generated by immunizing a rabbit with purified $(His)_6$-tagged Bm35, suggesting that bm35 is synthesized in the late stage of BmNPV infection cycle. Bm35 was not a structural component associated with budded virus (BV) and occlusion derived virus (ODV). These data indicated that bm35 is a functional gene in the BmNPV life cycle.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.1
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• pp.25-29
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• 2001

We have cloned and characterized an antiapoptotic gene, p35, which blocks apoptosis, from Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. The 897 bp p35 has an open reading frame of 299 amino acids. The BmNPV-K1 p35 showed a high identity to Autographa californica nuclear polyhedrosis virus and BmNPV T3 strain. The BmNPV-K1 p35 was different from the amino acid sequences of BmNPV T3 at 6 positions. The p35 gene of BmNPV-K1 was 99.2% identical at the nucleotide level and 98% identical at the amino acid level to BmNPV T3. The location of p35 gene in the BmNPV-K1 genome was confirmed by Southern blot analysis and its expression patterns at the transcriptional level in the infected cells were con- firmed by Northern hybridization analysis.

• International Journal of Industrial Entomology and Biomaterials
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• v.15 no.1
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• pp.35-38
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• 2007

In this study, ORF 46 of Bombyx mod nucleopolyhedrovirus(Bm46) fused with EGFP was expressed in Bombyx mod cell lines, larvae and pupae by BmNPV Bacmid system. Bm46 and EGFP were cloned into donor plasmid pFastBacHTb, which was transformed to competent DH10B cells containing helper and BmNPV bacmid by site-specific transposition. Recombinant bacmid was used to transfected BmN-4 cells to produce the recombinant baculovirus vBm-Bm46-EGFP. Recombination virus was injected into silkworm larvae and pupae. The expression of the fusion protein was monitored by examining green fluorescence using a fluorescent microscope. Intense fluorescence in cells and silkworm was observed at 4 days post-infection, indicating the Bm46-EGFP fusion gene was expressed successfully.

• The Journal of the Korea institute of electronic communication sciences
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• v.6 no.5
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• pp.621-627
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• 2011

In this paper, we analysed performance for on-wafer GaN HEMT using load-pull in X-band, and studied optimum impedance point based on analysis result. We suggested method of optimum performance device by analysis of optimum impedance for solid state device on-wafer condition before packaging. The measured device is gate length 0.25um, and gate width is 400um, 800um. device 400um is performed $P_{sat}$=33.16dBm, PAE=67.36%, Gain=15.16dBm, and device 800um is performed $P_{sat}$=35.91dBm, PAE=69.23%, Gain=14.87dBm.

• Proceedings of the Korea Society for Industrial Systems Conference
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• 2000.11a
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• pp.35-65
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• 2000

BM 특허란 무엇인가\ulcorner 특허법 제 2 조 발명이라 함은 자연법칙을 이용한 기술적 사상의 창작으로 고도한 것을 말하며 특허 요건으로는 산업상 이용가능성, 신규성, 진보성이 이에 속한다. BM 특서는 IT를 이용하여 실현한 새로운 비즈니스 시스템 혹은 방법에 관한 특허를 말한다.(중략)

• Journal of Advanced Navigation Technology
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• v.27 no.6
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• pp.849-854
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• 2023

In this paper, a high-efficiency power amplifier was designed by applying the operating point Class J using LDMOS(laterally diffused metal oxide semiconductor) and optimizing the output matching circuit so that the second harmonic impedance becomes the reactance impedance. The designed power amplifier has a frequency of 108 ~ 110 MHz, Characteristics of PAE(power added efficiency) is 71.5% at P_SAT output (54.5 dBm), 55.5% at P_1dB output (51.5 dBm), and 24.38% at 45 dBm. The CSB(carrier with sideband) amplifier, which is the reference signal in the spatial modulation method, has an operating output of 45 dBm ~ 35 dBm, and linear SDM(sum in the depth of modulation) characteristics(40% ± 0.3%) were obtained. We measure the characteristics in amplitude modulation according to the bias operating point of the power amplifier for CSB and propose the optimal operating point to obtain linear modulation characteristics.

• Journal of Navigation and Port Research
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• v.35 no.10
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• pp.817-822
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• 2011

Lab scale experiment study was carried out for biological treatment process development in cruise. SBR(Sequence Batch Reactor) process with BM(Beneficial Microorganisms) was investigated for practical application on shipboard sewage treatment. From the results it was suggested that SBR process with BM might be a suitable process for cruise sewage treatment in terms of decrease in odorous compounds, maintenance of useful microorganisms and creating special environmental conditions. By adding BM to SBR system, odor unit of sulfur compounds was about 20 times reduced.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.1A
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• pp.85-90
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• 2008

Effects of the emitter degeneration on linearity have been investigated in SiGe HBT double-balanced up-converters with the Gilbert-cell structure. The emitter-coupled degeneration resistors have been optimized for high P1-dB and IP3 through the nonlinear harmonic-balance simulation. Two types of up-converter MMICs fabricated in $0.35{\mu}m$ Si-BiCMOS process were measured to verify the simulation results. The up-converter without the degeneration resistors produces a P1-dB of -13 dBm with an OIP3 of 3.7 dBm, while the up-converter with the degeneration resistors produces a P1-dB of -10 dBm with an OIP3 of 8.7 dBm.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.6
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• pp.1166-1173
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• 2009

In this paper, we studied on the power amplifier which has the output of the optimal to 100MHz-2GHz band. Optimal output power match was fabricated using the two types; one is the linear tapering and the other is the impedance transformer. In the case of output power match using linear tapering, output power was 35.35dBm at 2GHz and 31.41dBm at 100MHz. The other case of output match using impedance transformer, output power was 34.8dBm at 2GHz and 33.25dBm at 100MHz. Comparison of the results in the two cases, impedance transformer type present the improved results by l.84dB of output power.

• Korean Journal of Microbiology
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• v.34 no.3
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• pp.137-143
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• 1998

Glycosides were synthesized using transglycosylation reaction of amylase in water system. The glycosides synthesized in water phase by a-amylase with starch as a glycosyl donor and benzylalcohol as an acceptor were identified as benzylalcohol-${\alpha}$-glucoside (BG) and benzylalcohol-${\alpha}$-maltoside (BM) of which one molecule of benzylalcohol was bound to 1-OH of glucose. The final products were BG in reaction system of pH 5.0, and BM in that of pH 8.0. The transglycosylation reaction by ${\alpha}$-amylase were carried out in water system containing 50 mg starch, 50 mg benzylalcohol, and 10 units enzyme at $30-35^{\circ}C$ for 3 days. The synthesized BG was hydrolyzed to glucose and benzylalcohol by ${\alpha}$-glucosidase, while ${\alpha}$-amylase hydrolyzed BM to glucose and benzylalcohol-${\alpha}$-glucoside in pH 5.0. Maltotriose resemble structurally to BM was rapidly hydrolyzed to glucose and maltose by ${\alpha}$-amylase at pH 5.0, being slightly hydrolyzed at pH 8.0, but not transglycosylated in present of benzylalcohol.