International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Characterization of the Open Reading Frame 35 of Bombyx mori Nucleopolyhedrovirus

  • Zhu, Ying Min (Institute of Life Science, Jiangsu University) ;
  • Li, Guo Hui (Institute of Life Science, Jiangsu University) ;
  • Yao, Qin (Institute of Life Science, Jiangsu University) ;
  • Chen, Ke Ping (Institute of Life Science, Jiangsu University) ;
  • Guo, Zhong Jian (Institute of Life Science, Jiangsu University)
  • Received : 2010.10.04
  • Accepted : 2010.12.02
  • Published : 2010.12.31
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Abstract

Open reading frame 35 (bm35) of the Bombyx mori nucleopolyhedrovirus (BmNPV) is a special gene whose homologues are only found in some group-I nucleopolyhedroviruses, suggesting that bm35 plays a specific role in the viral life cycle. This paper described the characterization of BmNPV bm35. Computerassisted sequence analysis shows that a putative RING finger motif is observed in the protein, Bm35 encoded by bm35. The coding sequence of bm35 was amplified and subcloned into the vector pET30a(+) and the $(His)_6$-tagged fusion protein His-Bm35 was expressed in the Escherichia coli BL21 (DE3) LysS cells. The bm35 transcript and Bm35 protein were detected in BmNPV-infected BmN cells at 12~48 h post infection (p.i.) by RT-PCR and Western blot analysis using the polyclonal antibody generated by immunizing a rabbit with purified $(His)_6$-tagged Bm35, suggesting that bm35 is synthesized in the late stage of BmNPV infection cycle. Bm35 was not a structural component associated with budded virus (BV) and occlusion derived virus (ODV). These data indicated that bm35 is a functional gene in the BmNPV life cycle.

Keywords

References

  1. Acharya A, Gopinathan KP (2002) Transcriptional analysis and preliminary characterization of ORF Bm42 from Bombyx mori nucleopolyhedrovirus. Virology 299, 213-224. https://doi.org/10.1006/viro.2002.1525
  2. Ahrens CH, Russell RL, Funk CJ, Evans JT, Harwood SH, Rohrmann GF (1997) The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome. Virology 229, 381-399. https://doi.org/10.1006/viro.1997.8448
  3. Ayres MD, Howard SC, Kuzio J, Lopez-Ferber M, Possee RD (1994) The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202, 586-605. https://doi.org/10.1006/viro.1994.1380
  4. Braunagel SC, Summers MD (1994) Autographa californica nuclear polyhedrosis virus, PDV, and ECV viral envelopes and nucleocapsids: structural proteins, antigens, lipid and fatty acid profiles. Virology 202, 315-328. https://doi.org/10.1006/viro.1994.1348
  5. Bulach DM, Kumar CA, Zaia A, Liang B, Tribe DE (1999) Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences. J Invertebr Pathol 73, 59-73. https://doi.org/10.1006/jipa.1998.4797
  6. Chen HQ, Chen KP, Yao Q, Guo ZJ, Wang LL (2007) Characterization of a late gene, ORF67 from Bombyx mori nucleopolyhedrocirus. FEBS Lett 581, 5863-5842.
  7. Chen YR, Wu CY, Lee ST, Wu YJ, Lo CF, Tsai MF, Wang CH (2008) Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus. J Gen Virol 89, 2315-2330. https://doi.org/10.1099/vir.0.2008/001412-0
  8. Daimon T, Katsuma S, Shimada T (2007) Mutational analysis of active site residues of chitinase from Bombyx mori nucleopolyhedrovirus. Virus Res 124, 168-175. https://doi.org/10.1016/j.virusres.2006.11.001
  9. de Jong JG, Lauzon HAM, Dominy C, Poloumienko A, Carstens EB, Arif BM, Krell PJ (2005) Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome. J Gen Virol 86, 929-943. https://doi.org/10.1099/vir.0.80490-0
  10. Du MF, Yin XM, Guo ZJ, Zhu LJ (2006) Characterization of a late gene, ORF60 from Bombyx mori nucleopolyhedrovirus. J Biochem Mol Biol 39, 737-742. https://doi.org/10.5483/BMBRep.2006.39.6.737
  11. Fan Q, Li S, Wang L, Zhang B, Ye B, Zhao Z, Cui L (2007) The genome sequence of the multinucleocapsid nucleopolyhedrovirus of the Chinese oak silkworm Antheraea pernyi. Virology 366, 304-315. https://doi.org/10.1016/j.virol.2007.04.027
  12. Gomi S, Majima K, Maeda S (1999) Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. J Gen Virol 80, 1323-1337. https://doi.org/10.1099/0022-1317-80-5-1323
  13. Grace TDC (1967) Establishment of a line of cells from the silkworm Bombyx mori. Nature 216, 613.
  14. Guo ZJ, Qiu LH, An SH, Yao Q, Park EY, Chen KP, Zhang CX (2010) Open reading frame 60 of the Bombyx mori nucleopolyhedrovirus plays a role in budded virus production. Virus Res 151, 185-191. https://doi.org/10.1016/j.virusres.2010.05.003
  15. Harrison RL, Bonning BC (2003) Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses. J Gen Virol 84, 1827-1842. https://doi.org/10.1099/vir.0.19146-0
  16. Harrison RL, Lynn DE (2007) Genomic sequence analysis of a nucleopolyhedrovirus isolated from the diamondback moth, Plutella xylostella. Virus Genes 35, 857-873. https://doi.org/10.1007/s11262-007-0136-6
  17. Hasnain SE, Jain A, Habib S, Ghosh S, Chatterji U, Ramachandran A, Das P, Venkaiah B, Pandey S, Liang B, Ranjan A, Natarajan K, Azim CA (1997) Involvement of host factors in transcription from baculovirus very late promoters - a review. Gene 190, 113-118. https://doi.org/10.1016/S0378-1119(96)00827-X
  18. Hefferon KL (2004) Baculovirus late expression factors. J Mol Microbiol Biotechnol 7, 89-101. https://doi.org/10.1159/000078652
  19. Hyink O, Dellow RA, Olsen MJ, Caradoc-Davies KMB, Drake K, Herniou EA, Cory JS, O'Reilly DR, Ward VK (2002) Whole genome analysis of the Epiphyas postvittana nucleopolyhedrovirus. J Gen Virol 83, 957-971.
  20. IJkel WFJ, Westenberg M, Goldbach RW, Blissard GW, Vlak JM, Zuidema D (2000) A novel baculovirus envelope fusion protein with a proprotein convertase cleavage site. Virology 275, 30-41. https://doi.org/10.1006/viro.2000.0483
  21. Imai N, Matsuda N, Tanaka K, Nakano A, Matsumoto S, Kang W (2003) Ubiquitin ligase activities of Bombyx mori nucleopolyhedrovirus RING finger proteins. J Virol 77, 923-930. https://doi.org/10.1128/JVI.77.2.923-930.2003
  22. Jehle JA, Blissard GW, Bonning BC, Cory JS, Herniou EA, Rohrmann GF, Theilmann DA, Thiem SM, Vlak JM (2006) On the classification and nomenclature of baculoviruses: a proposal for revision. Arch Virol 151, 1257-1266. https://doi.org/10.1007/s00705-006-0763-6
  23. Katsuma S, Horie S, Daimon T, Iwanaga M, Shimada T (2006) In vivo and in vitro analyses of a Bombyx mori nucleopolyhedrovirus mutant lacking functional vfgf. Virology 355, 62-70. https://doi.org/10.1016/j.virol.2006.07.008
  24. Kelly BJ, King LA, Possee RD (2007) Introduction to baculovirus molecular biology; in Baculovirus and Insect Cell Expression Protocols. Murhammer DW (ed), pp. 25-53, Humana Press, Totowa, New Jersey.
  25. King LA, Possee RD (1992) The baculovirus expression system: a laboratory guide. Chapman and Hall, London, UK.
  26. Lauzon HAM, Jamieson PB, Krell PJ, Arif BM (2005) Gene organization and sequencing of the Choristoneura fumiferana defective nucleopolyhedrovirus genome. J Gen Virol 86, 945-961. https://doi.org/10.1099/vir.0.80489-0
  27. Muller R, Pearson MN, Russell RL, Rohrmann GF (1990) A capsid-associated protein of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata: genetic location, sequence, transcriptional mapping, and immunocytochemical characterization. Virology 176, 133-144. https://doi.org/10.1016/0042-6822(90)90238-M
  28. Nie ZM, Zhang ZF, Wang D, He PA, Jiang CY, Song L, Chen F, Xu J, Yang L, Yu LL, Chen J, Lv ZB, Lu JJ, Wu XF, Zhang YZ (2007) Complete sequence and organization of Antheraea pernyi nucleopolyhedrovirus, a dr-rich baculovirus. BMC Genomics 8, 248-261. https://doi.org/10.1186/1471-2164-8-248
  29. Oliveira JV, Wolff JL, Garcia-Maruniak A, Ribeiro BM, de Castro ME, de Souza ML, Moscardi F, Maruniak JE, Zanotto PM (2006) Genome of the most widely used viral biopesticide: Anticarsia gemmatalis multiple nucleopolyhedrovirus. J Gen Virol 87, 3233-3250. https://doi.org/10.1099/vir.0.82161-0
  30. Theilmann DA, Blissard GW, Bonning BC, Jehle JA, O'Reilly DR, Rohrmann GF, Thiem SM, Vlak JM (2005) Baculoviridae; in Virus taxonomy: the eighth report of the International Committee on Taxonomy of Viruses. Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds.), pp. 177-185, Elsevier Academic Press, New York, NY.
  31. Todd JW, Passarelli AL, Lu A, Miller LK (1996) Factors regulating baculovirus late and very late gene expression in transient-expression assays. J Virol 70, 2307-2317.
  32. Wang D, An SH, Guo ZJ, Xu HJ, Zhang CX (2005) Characterization of Helicoverpa armigera nucleopolyhedrovirus orf33 that encodes a novel budded virion derived protein, BVe31. Arch Virol 150, 1505-1515. https://doi.org/10.1007/s00705-005-0534-9
  33. Wang F, Yang LR, Tang XD, Mo JC, Yang WJ, Zhang CX (2006) The translational and transcriptional initiation sites of BmNPV lef-7 gene. Virus Genes 33, 351-357.
  34. Wang R, Deng F, Hou D, Zhao Y, Guo L, Wang H, Hu Z (2010) Proteomics of the Autographa californica nucleopolyhedrovirus budded virions. J Virol 84, 7233-7242. https://doi.org/10.1128/JVI.00040-10
  35. Xu HJ, Yang ZN, Wang F, Zhang CX (2006) Bombyx mori nucleopolyhedrovirus ORF79 encodes a 28-0kDa structural protein of the ODV envelope. Arch Virol 151, 681-695. https://doi.org/10.1007/s00705-005-0664-0
  36. Xu YP, Ye ZP, Niu CY, Bao YY, Wang WB, Shen WD, Zhang CX (2010) Comparative analysis of the genomes of Bombyx mandarina and Bombyx mori nucleopolyhedrovirus. J Microbiol 48, 102-110. https://doi.org/10.1007/s12275-009-0197-4
  37. Zanotto PMDA, Kessing BD, Maruniak JE (1993) Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. J Invertebr Pathol 62, 147-164. https://doi.org/10.1006/jipa.1993.1090