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Comparisons of UCP2 Polymorphism, Dietary Habits, and Obesity Index in Normal and Obese University Students (정상체중과 비만인 대학생의 UCP 2 유전자 다형성, 식습관, 비만도 및 체성분의 비교 연구)

  • Ahn, Myoung-Soo;Chang, In-Youb;Kim, Kyung-Hee
    • Journal of the Korean Society of Food Culture
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    • v.22 no.4
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    • pp.404-413
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    • 2007
  • This study was carried out to compare UCP2 polymorphism, dietary habits, and obesity index in normal and obese university students. The survey was carried out using self-questionnaires collected from the 126 normal and 60 obese university students. The results are summarized as follows. Breakfast was skipped in 43.7% of normal and 49.3% of obesity students and it appeared obese students eat faster than normal students. The percentage of weight control experience were 49.2 and 71.0 in the normal and obese students, respectively. Blood levels of lipid profiles(triglyceride, LDL cholesterol, and HDL cholesterol), hemoglobin, AST and ALT were anaylzed. In UCP2 genes, the frequency of deletion homozygote(DD) was 71.5%, heterozygote(DI) was 26.9% and insertion homozygote(II) was 1.6%. Plasma levels of triglyceride, total cholesterol, LDL cholesterol, and HDL cholesterol of normal students were 79.06, 172.25, 100.86 and 57.03 mg/dl, and those of obese students were 93.06, 173.22, 101.22 and 54.39 mg/dl, respectively. Blood parameters were in normal range in both group. Plasma triglyceride, total cholesterol and LDL cholesterol levels of obese students were higher than those of normal students. On the other hand, plasma HDL cholesterol levels of obese students were lower than those of normal students. Plasma levels of AST and ALT were in normal range in both group. However, AST and ALT levels of obese students were higher than that of normal students. Thus, it was recommended for them to have a nutritional education program to improve their dietary and living habits for obese students’health. Nutritional education program should also be organized practically and systematically.

Influence of metabolizable energy on blood biomarkers in duck under heat stress (대사에너지가 열 스트레스에 노출된 오리의 혈액 생체지표에 미치는 영향)

  • Sim, J.B.;Choi, D.H.;Kim, C.R.;Park, B.S.
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.1
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    • pp.132-141
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    • 2017
  • The objective of this study was to determine the influence of dietary metabolic energy (ME) on blood parameters in duck under heat stress. A total of 240 meat ducks Cherry valley (Anas platyrhynchos) were assigned into four treatment groups with a randomized block design for 42 days. The four treatments were: ME 2900 kcal/kg, ME 3000 kcal/kg, ME 3100 kcal/kg, and ME 3200 kcal/kg. Blood lipid profiles was higher in ME 2900 but lower in ME 3100 and ME 3200 than that of ME 3000 (p < 0.05). Blood aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were higher in ME 3100 and ME 3200 compared those in ME 3000 (p < 0.05). The blood red cell and platelet profiles were increased in ME 3100 and ME 3200, but reduced in ME 2900 compared to those in ME 3000 (p < 0.05). Among blood electrolytes, chloride ($Cl^-$) concentration was decreased in ME 2900 compared to that in ME 3000. Blood gas $PCO_2$ was reduced in ME 2900 compared to that in ME 3000 (p < 0.05). Blood immunoglobulin (IgG) level was reduced in ME 2900 compared to that in ME 3000 (p < 0.05). Level of stress hormone, corticosterone was increased in ME 2900, but decreased in ME 3100 and ME 3200 compared to that in ME 3000 (p < 0.05).

Effect of urea on blood-luminol chemiluminescence reaction (Urea가 혈액-luminol 화학발광에 미치는 영향)

  • Youngpyo Bae;Sungwook Hong
    • Analytical Science and Technology
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    • v.36 no.6
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    • pp.332-339
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    • 2023
  • This study aimed to verify the hypothesis that urea denatures hemoglobin in the blood, thereby exposing active sites of enzymes and enhancing the chemiluminescence of the blood-luminol reaction. When blood was pretreated with urea, higher concentrations of pretreatment urea or longer pretreatment times resulted in enhanced chemiluminescence in the blood-luminol reaction, supporting the above hypothesis. However, the chemiluminescence was enhanced when blood was treated with luminol mixed with an 8 M urea solution, although the fact that the time for urea to denature hemoglobin was shorter compared to when blood was pretreated with urea and followed by luminol. In addition, the chemiluminescence was enhanced when a transition metal without hemoglobin was reacted with urea-containing luminol. Based on these results, it is anticipated that urea not only denatures hemoglobin but also plays a role in the luminol-hydrogen peroxide reaction.

Comparative study of Photobacterium damselae subsp. damselae and Vibrios on pathogenicity in vivo (In vivo에서 Photobacterium damselae subsp. damselae와 Vibrio 속 세균의 병원성 비교)

  • Kwon, Mun-Gyeong;Cho, Byoung-Youl;Park, Soo-Il
    • Journal of fish pathology
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    • v.22 no.2
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    • pp.115-124
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    • 2009
  • Photobacterium damselae subsp. damselae and 4 Vibrio spp, V. anguillarum, V. splendidus, V. harveyi and V. ordalii, were isolated from the diseased olive flounders, Paralichthys olivaceus. The pathogenicity of the isolates were compared to mortality, blood biochemical contents, such as alanineaminotransferase(ALT), aspatate aminotransferase(AST), and cortisol level, and non-specific immune responses, nitroblue tetrazolium (NBT) reduction of macrophages and lysozyme activities of serum. The mortalities and levels of ALT, AST and cortisol of fishes infected with P. damselae were higher than those of others but significantly low in non-specific immune responses, NBT and lysozyme activities. These results suggest that P. damsela might produce damselysin having high hemolytic and phospholipase activities, correlated with the pathogenicity. P. damsela could also make an obstruction of internal organs, following increasing in the level of ALT and AST, and depression in host immunity caused by induced high levels of cortisol.

Effects of Using Far Infrared Ray (FIR) on Growth Performance, Noxious Gas Emission and Blood Biochemical Profiles in Broiler (원적외선 조사가 육계의 생산성, 유해가스 발생량 및 혈액의 생화학적 조성에 미치는 영향)

  • Son, Jang Ho
    • Korean Journal of Poultry Science
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    • v.42 no.2
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    • pp.125-132
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    • 2015
  • The Far Infrared Ray (FIR) is part of the natural energy as light spectrum of sunlight. Human can disentangle the colors within visible ray, but FIR is invisible to human sight because it has longer wavelength than visible ray. The effect of using FIR on broiler performance, blood biochemical profiles and fecal gas emission from litter. Day-old semi-broiler chicks (Ross ♂ ${\times}$ Hyline ♀) were obtained and assigned to eight pens, 2 replicates of white and green color LED light, and with FIR on each color light, in a 20L:4D of lighting program. The body weight gain and feed efficiency were tend to improve under the green color than white color, which were increased by exposing to FIR on both color light. Emission of ammonia and lower hydrocarbons from litter were not different from each color but there was a decrease by exposing to FIR regardless of light color. The level of blood aspartate aminotransferase (AST) tends to be decreased under green color than white color, and this tendency becomes more pronounced as exposing to FIR. Therefore significantly increased under white color without FIR than green color with FIR (P<0.05). The levels of albumin and immunoglobulin were not different from each color but there was an increase by exposing to FIR regardless of light color. In conclusion, exposing to Far Infrared Ray (FIR) when broiler raising, there is potential to increase broiler performance because of improvement of bioactivity and raising environment.

Effects of acute LeeKwaDoo administration on liver regeneration induced by partial hepatectomy in rats (부분 간절제술을 시행한 랫드에서 간재생에 관한 이과두주 급성투여에 따른 영향)

  • Cho Jin-Youn;Song Ji-Yae;Ahn Jae-Bum;Kim Hyun-Seok;Kim Min-Su;Im Hyun;Lim Jun-Sung;Shin Dong-Seok;Kim Hyun-Chul;Jung Ki-Soo;Shin Myung-Kyun;Lee Min-Jae
    • Korean Journal of Veterinary Service
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    • v.29 no.1
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    • pp.55-63
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    • 2006
  • Alcoholism and alcohol abuse are major public health concerns. This is linked to the injury of many organs, especially liver. Experiments were peformed to know the acute effects of LeeKwaDoo (LKD) induced by two-third partial hepatectomy (PH) in rats. In liver samples, regeneration parameters and histological assessment were performed. For the blood biochemical study, the blood were assayed with AST, ALT. The portal branch of liver lobes was ligated in the male Sprague-Dowley rats, two-thirds partial hepatectomies were also performed. It was estimated bodyweight and relative liver weight for the index of liver mass. For the marker of blood chemistry, we investigate the serum sample of rats and demonstrated the level of AST, ALT. Remaining tissues of liver developed as microscopic structures. Resection of the lobes in PH+LKD group resulted in a marked change of liver weight, blood chemistry and histological changes. The initiation of the proliferative response in PH group stimulated as well as reduction of the liver mass. On the other hands, the Initiation of the proliferative response in PH+LKD group delayed. Eventually, both PH group and PH+LKD group was restored relative liver weigh after 7 day. In conclusion, the acute adminstration of LKD seems to inhibit the initial response of liver regeneration through alcohol effects.

Comparison of Blood Gas, Biochemical Factors, and Hormone Concentrations, and Muscle Stress Response Factors of Chickens in General Farms and Animal Welfare Farms (일반농장과 동물복지농장 닭의 혈액 내 gas, 생화학인자, 호르몬 농도 및 근육 내 스트레스 반응인자 비교)

  • Kim, Hee-Eun;Park, Jin-Ryong;Lee, Jeong-Eun;Kang, Da-Rae;Jang, Ae-Ra;Choe, Ho-Sung;Shim, Kwan-Seob
    • Korean Journal of Organic Agriculture
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    • v.29 no.3
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    • pp.415-427
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    • 2021
  • It is generally recognized that high stocking density can increase the risk of immune dysfunction and reduction in productivity cause of elevated stress in broiler farm. The domestic livestock industry is implementing a certification system for animal welfare and livestock farms to reduce the problem of dense breeding. This study compared broiler from the general farm and animal welfare farm to confirm the effectiveness of animal welfare farms against stress. Gas concentrations in a farm of general and animal welfare farms were analyzed, blood (n=10) of the 28-day-old broiler was collected, the partial pressure of the gas in the blood was analyzed using whole blood, and biochemical factors and hormones were analyzed using the serum. Glycogen and L-lactate are analyzed using ELISA kit on finely pulverized muscles (n=10). CO2 and NH3 levels in the farm were significantly higher in general farm. Partial carbon dioxide tension (PCO2) in blood was significantly higher and partial oxygen tension (PO2) was significantly lower in the general farm. Creatinine (CREA), glucose (GLU), aspartateaminotransferase (AST), and creatinekinase (CK) in the blood were significantly higher in the general farm, and total protein (TP) and globulin (GLOB) were significantly higher in animal welfare farm. Blood corticosterone and norepinephrine were significantly higher in general farm and epinephrine was significantly higher in animal welfare farm. These results imply that animal welfare farm provides a stress-reduced breeding environment.

Effects of Paljin-Tang on the Blood of Arsenic-poisoned Rats (팔진탕이 비소 중독된 흰쥐의 혈액에 미치는 영향)

  • Suh, Eun-Sil;Kim, Hoon;Lim, Jong-Pil
    • Korean Journal of Pharmacognosy
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    • v.29 no.4
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    • pp.379-383
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    • 1998
  • Sodium arsenate and Paljin-Tang extract (PJT), a herbal restorative were treated p.o. 20 mg/kg and 500 mg/kg, respectively, and concurrently to rats, and examined the biochemical parameters in blood. The values of white blood cell (WBC), red blood cell (RBC), hemoglobin (Hgb) and hematocrit (Hct) in each group did not show significant variance. The value of aspartate aminotrasferase(AST) of arsenic-treated group was increased for 2 weeks significantly while that of the group of concurrent administration with PJT became low significantly compared with arsenic-treated group and the value of alkaline phosphatase (ALP) of arsenic-treated group was decreased while that of the group of concurrent administration with PJT was increased significantly compared with arsenic-treated group. The value of glucose (Glu) was increased and those of lactic dehydrogenase (LDH), blood urea nitrogen (BUN) and triglyceride (TG) were decreased at first but increased later while the group of concurrent administration with PJT showed significant recovery from the toxicity of arsenic.

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Effects of Chitosan Oligosaccharide Supplementation on Blood Glucose, Lipid Components and Enzyme Activities in Hyperglycemic Rats (키토산 올리고당이 당뇨성 흰쥐의 혈당과 혈중 지질성분 및 효소활성에 미치는 영향)

  • Kim, Han-Soo;Seong, Jong-Hwan
    • The Korean Journal of Food And Nutrition
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    • v.21 no.3
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    • pp.328-335
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    • 2008
  • The principal objective of this study was to assess the effects of chitosan oligosaccharide supplementation on the improvement of blood glucose, lipid components and enzyme activities in the serum of streptozotocin(STZ, 55 mg/kg B.W., I.P. injection)-induced hyperglycemic rats fed on experimental diets for 5 weeks. The concentrations of blood glucose, total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free cholesterol, cholesteryl ester ratio, triglycerides(TG) and phospholipids(PL) in serum were remarkably higher in the hyperglycemic group(group BSW) and STZ(I.P.)+chitosan oligosaccharide supplementation group(group ECW) than those in the control group(group BW, basal diet+water). However the concentrations of blood glucose, total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, tree cholesterol, cholesteryl ester ratio, TG and PL in serum were lower in the ECW group than in the BSW group, whereas the ratio of HDL-cholesterol concentration to total cholesterol and HDL-cholesterol concentration in the ECW group were higher than in the BSW group. The activities of alkaline phosphatase(ALP) and aminotransferase(AST, ALT) in serum were lower in the ECW group than in the hyperglycemic BSW group. The results shown above suggested that chitosan oligosaccharide supplementation effectively improves blood glucose, lipid composition and enzyme activities in the sera of STZ-induced hyperglycemic rats.

Evaluation of the Relationship between the Results of Blood Test and Sasang Constitution (사상체질과 혈액검사 결과의 연관성 평가)

  • Jeong, Mi Kyung;Youn, Sang Jun;Jun, Chan Yong;Park, Jong Hyeong;Choi, You Kyung
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.26 no.6
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    • pp.964-969
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    • 2012
  • This study aimed to evaluate the relationship between the results of blood test and Sasang constitution. We performed blood test of 2,387 university students in Health examination. Sasang constitution was diagnosed by using the Questionnaire of Sasang Constitution Class II(QSCC II). All the data were analysed statistically by descriptive statistics, ANOVA and logistic regression analysis. The Taeeumin group showed significantly higher AST, ALT, GGT, LDH, CPK, creatinine, uric acid, total cholesterol, TG, and LDL levels than other groups, while having a lower HDL level. According to logistic regression analysis, Hb, RBC, uric acid, creatinine were effective common factors for classifying each constitution groups. In the results of this study, there were significant differences in the results of blood test between the three constitutions. But the blood test was insufficient as an objective indicator for discriminating Sasang constitutions.