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Comparative study of Photobacterium damselae subsp. damselae and Vibrios on pathogenicity in vivo  

Kwon, Mun-Gyeong (Pathology division, National Fisheries Research and Development Institute)
Cho, Byoung-Youl (Ulsan institute of health and environment)
Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.22, no.2, 2009 , pp. 115-124 More about this Journal
Abstract
Photobacterium damselae subsp. damselae and 4 Vibrio spp, V. anguillarum, V. splendidus, V. harveyi and V. ordalii, were isolated from the diseased olive flounders, Paralichthys olivaceus. The pathogenicity of the isolates were compared to mortality, blood biochemical contents, such as alanineaminotransferase(ALT), aspatate aminotransferase(AST), and cortisol level, and non-specific immune responses, nitroblue tetrazolium (NBT) reduction of macrophages and lysozyme activities of serum. The mortalities and levels of ALT, AST and cortisol of fishes infected with P. damselae were higher than those of others but significantly low in non-specific immune responses, NBT and lysozyme activities. These results suggest that P. damsela might produce damselysin having high hemolytic and phospholipase activities, correlated with the pathogenicity. P. damsela could also make an obstruction of internal organs, following increasing in the level of ALT and AST, and depression in host immunity caused by induced high levels of cortisol.
Keywords
Photobacterium damselae subsp. damselae; Vibrio; Pathogenicity; Flounder;
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