• Journal of the Mineralogical Society of Korea
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• v.12 no.1
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• pp.43-53
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• 1999

The medical mineral menas a single mineral or a complex of minerals. It is natural material. using the medical action of he major or the minor elements, and traditional medicine stuff which has been used since long time ago. Jusa, cinnabar as the mineral name, is the product of the hydrothermal process. It is used to relax the body and cure high blood pressure, apoplexy and cardiopathy. Jusais the major component of "An shin hwan" and "Woo hwang chung shim hwan" nowadays because it has such an excellent calm effect. In addition, it is used to cure cancers such as esophageal cancer and gastric cancer. Jusa composed of mercuric sulfide causes mercury poisoning such as Minamata disease. It is dealt with mineralogical property and chemical composition medical stuff in Korea and China, as well asmercury poisoning and medical action of Jusa in this study. In order to predct accumulation of the interior of the body of the major and minor elements in Jusa, leaching experiment of Jusa by artificial gastric juice was done as well as thermodynamic reaction modelling to know concentration of each species of body fluid. The minor elements of 24 species such as As, Pb, Cd, a and Fe by leaching reaction of Jusa and artificial gastric juice were leached. We can know the fact that as is less than 1 ppm, Hg is less than 25 ppm and Cd and m are not detected. In addition, mercury exists as species of Hg2+, HgCl+, HgCl2, HgCl3-, HgCl42-, HgClOH, HgS(H2S)2, Hg(HS)3-, HgS22-, HgOH and Hg(OH)2 by reaction modelling between Jusa and artificial gastric juice. The concentration of sulfide complexes is 24.2 ppm and that of others is less than 10 ppm. According to increasing pH, the concentration of HgS(H2S)2, Hg(HS)3+, HgS22- and Hg(OH)2 increases, whereas the concentration of HgCl+, HgCl2, HgCl3- and HgCl42- decreases. Therefore, Jusa is very useful for the development of new medicine because it is possible to predict formation of the body species and species accumulation on mercury known as a toxic element and concentration changes of toxicity and efficiency.city and efficiency.

• Korean Journal of Materials Research
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• v.24 no.12
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• pp.671-676
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• 2014

It is known that bones get damaged by accidents and aging. Since the discovery of Bioglass, various kinds of ceramics have been also found to bond to living bone; some of these ceramics are already being clinically used as bone-repairing materials. In the present study, antibacterial calcium silicate gel ($Ag-30CaO{\cdot}70SiO_2$ gel) was prepared by sol-gel method in order to control the microstructure, which is related to the dissolution rate and induction period of apatite formation in body environment. In addition, biological $Ag-30CaO{\cdot}70SiO_2$ is tested. This was done to impart antimicrobial activity to the $30CaO{\cdot}70SiO_2$. Ag ion was added during sol-gel synthesis to replace the $H_2O$ added during the making of the $30CaO{\cdot}70SiO_2$ gel, which has silver solutions of various concentration. After the sol-gel process, 1N-$HNO_3$ solution was used to wash the gel when synthesizing the gel, in order to maintain the porous structure and remove PEG, water soluble polymers. Then, the apatite forming ability of the sol-gel derived CaO-$SiO_2$ gels was investigated using simulated body fluid (SBF), which had almost the same ion concentration as that of human blood plasma. The gels were analyzed by FT-IR spectroscopy, SEM observation, XRD, and fluorescent microscopy. The apatite was successfully created even after washing the gel; apatite is present in an amorphous state, and was found to affect the concentration of the Ag ion in cells in MC3T3 live & dead assay results. From these results, it is suggested that a good material that can be used to repair defects of nature bone is $Ag-30CaO{\cdot}70SiO_2$ gel.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.7
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• pp.1139-1146
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• 2004

The purpose of this study was to investigate the possibility of Meles meles oil as an functional resource. To assess the effects of Meles meles oil in 25 non-insulin dependent diabetes mellitus (DM) persons, we examined changes of fat intake level, hematological and chemical variables, serum DM indices and lipid contents during the Meles meles oil supplementation. Polyunsaturated fatty acid and $\omega$3 fatty acid intake were significantly increased by Meles meles oil intakes. The levels of LDL-cholesterol and triglyceride were significantly decreased while HDL-cholesterol was significantly increased. Iron status improved during Meles meles oil intakes. These results show that modest dose of Meles meles oil supplementation can decrease serum triglyceride, cholesterol level without any changes in blood glucose level in NIDDM patients. These results indicated that Meles meles oil diet is effective therapeutic regimen for the control of metabolic derangements in diabetes mellitus. Also, these results imply that Meles meles oil can be used as possible food resources and functional food materials. However, large amounts of Meles meles oil should be used cautiously in NIDDM patients.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.8
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• pp.1273-1278
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• 2004

The change of tissue lipid levels and fatty acid compositions in alloxan-induced diabetes was studied in rats (SD, male) in order to examine the pathway of diabetic complications. Rats were injected with alloxan 20 mg/kg BW or 40 mg/kg BW to induce diabetes. In rats injected with alloxan (40 mg/kg BW), the body weight was significantly decreased, food intake and liver weight per 100 g (BW) were significantly increased, compared with other groups. The blood glucose levels were apparently elevated as about 2 times in rats injected with alloxan (40 mg/kg BW) than the other groups. The concentrations of serum total cholesterol, triglyceride and HDL-cholesterol were not significantly different among the groups. However, the levels of serum triglyceride tended to increase according to amount of alloxan injected. Liver cholesterol levels were significantly decreased in rats injected with alloxan (40 mg/kg BW) compared with other groups, but triglyceride levels of those were not significantly different among groups. Concerning the fatty acid compositions of serum, liver, kidney, spleen phosphatidylcholine, the percentage of linoleic acid in rats injected with alloxan (40 mg/kg BW) was significantly increased, while that of arachidonic acid was significantly decreased compared with the other groups. Therefore, the ratios of arachidonic/linoleic acid in tissue phosphatidylcholine tended to be low in rats injected with alloxan (40 mg/kg BW) and especially significant low levels were found in serum and spleen. Thus, it was suggested that insulin deficiency can affect on fatty acid biosynthesis and induce diabetic complications.

• Journal of Pharmaceutical Investigation
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• v.41 no.1
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• pp.31-36
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• 2011

$5HT_{2C}$ receptor among fourteen 5-HT subtypes plays important roles in several disorders such as depression, anxiety, epilepsy, schizophrenia and sleep disorders. The purpose of the study is to investigate pharmacokinetic parameters and bioavailability of a newly synthesized selective agonist of $5-HT_{2C}$ receptor, KOPC-20010 (KP10) in rats after intravenous and oral administration for the development of therapeutic anti-obesity agents. KP10 was administered orally (40 mg/kg) or intravenously (20 mg/kg), blood was collected via a catheter, and analyzed by GC/MSD. The calibration curve of KP10 in plasma and urine showed high linearity ($r^2$ >0.999). The retention times of KP10 in plasma and urine were 8.7 and 9.7 min, respectively. After oral administration of 40 mg/kg, pharmacokinetic parameters were calculated as follows; $C_{max}$ value was $1242.9{\pm}1195.5$ ng/mL at $1.1{\pm}0.6$ hr ($T_{max}$). $AUC_{0->24hr}$ and $AUC_{0>{\infty}}$ were $8034.2{\pm}960.7$ and $10464.1{\pm}681.5\;ng{\cdot}hr/mL$, respectively. The terminal half-life was $21.9{\pm}7.6$ hr. $AUC_{0->24hr}$ and $AUC_{0>{\infty}}$ were $4292.4{\pm}523.0$ and $6111.2{\pm}756.2\;ng{\cdot}hr/mL$, respectively, after 20 mg/kg of intravenous administration. The terminal half-life after intravenous administration was $25.1{\pm}9.4$ hr. Bioavailability of KP10 was determined to 86%. The excretion amount into the urine within 48 hr was approximately 4.7 to 6.7% of the dose administered. These data may be beneficial to the anti-obesity drug development of KP10.

• The Korean Journal of Nuclear Medicine
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• v.30 no.3
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• pp.344-350
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• 1996

Purpose : Noninvasive imaging of tumor cell proliferation could be helpful in the evaluation of tumor growth potential and could provide an early assessment of treatment response. Radiolabeled thymidine, uridine and adenosine have been used to evaluate tumor cell proliferation. These nucleoside analogs are incorporated into DNA during proliferation. Iodine-131-Iodomethyluridine, an analog of Iodine-131-Iododeoxyuridine, is also involved in DNA/RNA synthesis. The purpose of this study was to develop Iodine-131-Iodomethylurdine and image tumor proliferation using Iodine-131-Iodomethyluridine. Materials and Methods : Radiosynthesis of Iodine-131-5-Iodo-2'-O-methyluridine (Iodine-131-Iodomethyluridine) was prepared from 10 mg of 2'-O-methyluridine(Sigma chemical Co., St. Louis, Missouri) and 2.1 mCi(SP. 10Ci/mg) of Iodine-131-labeled sodium iodide in $100{\mu}l$ of water using iodogen reaction. Female Fischer 344 rats were inoculated in the thigh area with breast tumor cells(13765 NF, $10^5$ cells/rat S.C.). After 14 days, the Iodine-131-Iodomethyluridine $10{\mu}Ci$ was injected to three groups of rats(3/group). The percent of injected dose per gram of tissue weight was determined at 0.5-hours, 2-hours, 4-hours, and 24-hours respectively. Tumor bearing rats after receiving Iodine-131-Iodomethyluridine($50{\mu}Ci$ IV) were euthanized at 2 hours after injection. Autoradiography was done using freeze-dried $50{\mu}m$ coronal section. After injection of Iodine-131- Iodomethyluridine ($10{\mu}Ci$/rat, IV) in three breast tumor-bearing rats, planar scintigraphy was taken at 45 minutes, 90 minutes and 24 hours. Results : Iodine-131-Iodomethyluridine was conveniently synthesized using iodogen reaction. The biodistribution showed fast blood clearance and the tumor-to-tissue uptake ratios showed that optimal imaging time was at 2 hours postinjection. Autoradiogram and planar scintigram indicated that tumor could be well visualized. Conclusion : The findings suggest that Iodine-131-Iodomethyluridine, a new radio-iodinated nucleoside, has potential use for evaluation of active regions of tumor growth.

• Applied Biological Chemistry
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• v.46 no.2
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• pp.134-139
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• 2003

To establish the fertilization condition to increase the productivity of Chasanthemum boreale M. with high quality, the effects of three nutrients (N, P, K) on the yields and the effective components were investigated in the pot scale. NPK was applied by chemical fertilizers with $(N-P_2O_5,-K_2O=250-160-160\;kg/ha)$ as a main treatment, and NP $(N-P_2O_5,-K_2O=250-160-160\;kg/ha)$, NK $(N-K_2O =250-160\;kg/ha)$, and PK $(P_2O_5K_2O=160-160\;kg/ha)$ treatments were settled as comparison. Dry yields of C. boreale M. was increased significantly to 4 fold higher by nitrogen. Nitrogen increased apparently plant growth and inorganic nutrient uptake. In the flower, which is most useful and edible part as a herbal medicine, main amino acids were glutamic acid and aspartic acids, and the total content was increased significantly by three elements of application. In addition, the content of cumambrin A, which is known to have the effect of blood-pressure reduction, was increased source to 6.2 times by nitrogen higher than that in PK treatment. Potassium was more effective in biosynthesis of cumambrin A than phosphorus, but the biological pathway was not clear, still.

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.77-77
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• 2002

본 실험은 한우 종빈우의 임신말기에 Se과 Vit. E 투여가 종빈우의 분만 후 번식기능에 미치는 요인을 검토하였고, 임신말기 Se과 Vit. E 를 투여한 한우 종빈우에서 태어난 송아지의 발육능력을 조사하고, 혈액화학치와 혈중 Vit. E 농도에 미치는 영향을 검토하였다. 1. 분만 l 개월전 Se과 Vit E 을 투여한 한우 종빈우에서 태어난 송아지의 발육성적을 조사한 결과, 생시체중은 투여구가 대조구보다 높은 성적을 나타냈다 (P＜0.05). 또한 이유시 체중과 일당증체량도 투여구가 대조구 보다 다소 높은 성적을 나타냈으나 처리구간 유의적인 차이는 인정되지 않았다 (P＞0.05). 한편 종빈우의 번식능력을 조사한 결과, 투여구가 대조구보다 초발정이 빨리 도래하였고, 수태 당 인공수정 횟수도 적었으나, 처리구간 유의적인 차이는 인정되지 않았다 (P＞0.05). 2. Se과 Vit. E를 분만 2 개월전 4 회 투여한 한우 종빈우로부터 태어난 송아지의 발육성적을 조사한 결과, 생시체중, 이유시체중 및 일당증체량은 투여구가 대조구보다 높은 성적을 나타냈으나, 처리구간 유의적인 차이는 인정되지 않았다 (P＞0.05). 한편, 한우 종빈우의 번식능력을 조사한 결과, 분만 후 초발정은 투여구가 대조구보다 초발정 발현이 빨랐으며 수태 당 인공수정 횟수는 투여구가 대조구에 비해 적었으나 처리구간 유의적인 차이는 인정되지 않았다 (P＞0.05). 3. Se과 Vit. E를 분만 1개월전 2 회 투여한 한우 종빈우에서 태어난 송아지의 혈액성분에 미치는 영향을 검토한 결과, 평균 혈중 albumin 은 Vit. E 2000IU 투여구가 여타구보다 높은 성적을 나타냈으며 (P＜0.05), 평균 혈중 cholesterol이 함량 또한 Vit. E 1500IU 투여구가 여타구보다 높게 나타났다 (P＜0.05). 그러나 혈중 BUN, creatinine, glucose, IP, calcium, TP 및 triglycerides 함량은 처 리 구간 유의 적 인 차이 는 없었다 (P＞0.05). 4. Se과 Vit. E를 분만 2 개월 전 4 회 투여한 한우 종빈우에서 태어난 송아지의 혈액성분에 미치는 영향을 검토한 결과, 평균 혈중 albumin, cholesterol, BUN, creatinine, glucose, IP, calcium, TP 및 triglycerides 함량은 모든 처리구에서 유의적인 차이가 인정되지 않았다 (P＞0.05). 5. 분만 l 개월 전 Se과 Vit. E를 투여한 한우 종빈우로부터 태어난 송아지의 혈중 Vit. E 농도를 측정한 결과, 평균 혈중 Vit. E 농도는 투여구가 대조구보다 다소 높은 농도를 나타냈으나 처리구간 유의적인 차이는 없었다 (P＞0.05). 6. 분만 2 개월 전에 Se과 Vit. E를 투여한 한우 종빈우로부터 태어난 송아지의 혈중 Vit. E 농도에 미치는 효과를 검토한 결과, 투여구가 대조구보다 다소 높은 농도를 나타냈으나 처리구간 커다란 차이는 없었다 (P＞0.05).

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.76-76
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• 2002

본 연구는 Se과 Vit. E 투여수준이 한우 종빈우 번식효율에 미치는 영향을 규명하기 위하여, 한우 종빈우의 임신말기에 적정량의 Se와 Vit .E를 투여하여, 종빈우의 혈액화학치 변화 및 혈중 Vit. E 농도변화를 규명하였다. 1. 분만 1개월 전 Se 과 Vit. E 투여가 한우 종빈우의 혈액성분에 미치는 영향을 검토한 결과, 평균 혈중 albumin 과 calcium 함량은 각각 투여구가 대조구보다 유의적으로 높았으며 (P＜0.05), 평균 혈중 IP 함량은 각각 Se 과 Vit. E 혼합투여구가 여타구보다 높은 성적을 나타냈다 (P＜0.05). 그러나 평균 혈중 BUN, creatinine, glucose, cholesterol 및 triglycerides 의 함량은 처리구간 유의적인 차이가 인정되지 않았다 (P＞0.05). 2. 분만 2개월 전 Se과 Vit. E 투여가 한우 종빈우의 혈액성분에 미치는 영향을 검토한 결과, 혈중 평균 albumin, BUN, cholesterol, creatinine, glucose, IP, TP, triglycerides 및 Ca 함량은 처리구간 유의적인 차이는 인정되지 않았다 (P＞0.05). 3. 한우 종빈우의 분만 l 개월전 Se 및 Vit. E 투여가 한우 종빈우의 혈중 Vit. E 농도에 미치는 영향을 검토한 결과, 평균 혈중 Vit. E 농도는 투여구가 대조구보다 다소 높은 농도를 나타냈으나, 처리구간 유의적인 차이는 인정되지 않았다 (P＞0.05). 4. 한우 종빈우에서 분만 2 개월전 Se 및 Vit. E 투여가 혈중 Vit. E 농도에 미치는 영향을 검토한 결과, 평균 혈중 Vit. E 농도는 처리구간 유의적인 차이가 없었으며 (P＞0.05), 처리구간 투여횟수의 증가에도 불구하고 혈중 Vit. E 농도는 커다란 차이가 없었다.atform by investigating current and future e-business services platforms involved in domestic and global international banks. Several financial interchange standards which are involved in B2B business of e-procurement, e-placement, e-payment are also investigated.. monocytogenes, E. coli 및 S. enteritidis에 대한 키토산의 최소저해농도는 각각 0.1461 mg/mL, 0.2419 mg/mL, 0.0980 mg/mL 및 0.0490 mg/mL로 측정되었다. 또한 2%(v/v) 초산 자체의 최소저해농도를 측정한 결과, B. cereus, L. mosocytogenes, E. eoli에 대해서는 control과 비교시 유의적인 항균효과는 나타나지 않았다. 반면에 S. enteritidis의 경우는 배양시간 4시간까지는 항균활성을 나타내었지만, 8시간 이후부터는 S. enteritidis의 성장이 control 보다 높아져 배양시간 20시간에서는 control 보다 약 2배 이상 균주의 성장을 촉진시켰다.차에 따른 개별화 학습을 가능하게 할 뿐만 아니라 능동적인 참여를 유도하여 학습효율을 높일 수 있을 것으로 기대된다.향은 패션마케팅의 정의와 적용범위를 축소시킬 수 있는 위험을 내재한 것으로 보여진다. 그런가 하면, 많이 다루어진 주제라 할지라도 개념이나 용어가 통일되지 않고 사용되며 검증되어 통용되는 측정도구의 부재로 인하여 연구결과의 축적이 미비한 상태이다. 따라서, 이에 대한 재고와 새로운 방향 모색이 필요하다고 사료된다.로 사료되며, 임신관련 cytokine에 대한 다양한

• Journal of Korean Neurosurgical Society
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• v.29 no.5
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• pp.664-667
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• 2000

Objective : Excessive bleeding from the exposed cancellous bone surface may cause serious problem such as hematoma formation, infection, transfusion reaction during operation and postoperative period. There are several kinds of bleeding control agent on the cancellous bone surface including bone wax, gelatin sponge ($Gelfoam^{(R)}$), oxidized cellulose($Oxycel^{(R)}$, $Surgicel^{(R)}$), thrombin, microfibrillar collagen($Avitene^{(R)}$) etc. In the past, bone wax was used to control bone bleeding but it is associated with increased infection rate and fusion failure. Recently, gelfoam paste has been used to control cancellous bone bleeding. We controlled the cancellous bone bleeding with the mixture of gelfoam powder and thrombin powder. Material and Methods : Seventeen patients of posterior fusion on the 4 motion segments of thoracolumbar spine were selected to compare the result of bone bleeding control. In the test group of 9 patients, the cancellous bone bleeding was controlled with the mixture of Gelfoam and thrombin powder during operation. In the control group of 8 cases, no chemical hemostatic agent was used to manage the bone bleeding during operation. We calculated the total amount of bleeding from cancellous bone surface during and after operation in the two groups and compared their statistic significance of the result which was judged by student t-test. Results : The average amount of intraoperative bleeding was 1825ml in control group, 811ml in test group(p<0.01). The amount of postoperative bleeding was 943ml in control group and 812ml in test group, there were no significant difference in the amount of bleeding during postoperative period between two groups(p>0.5). Total amount of blood was decreased in as much as 1150ml in test group(p<0.01). Conclusion : We concluded that the application of the mixture of thrombin and gelfoam powder on the cancellous bone surface is the effective control method of cancellous bone bleeding for multilevel posterior spinal fusion.