• Fisheries and Aquatic Sciences
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• 제7권2호
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• pp.58-63
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• 2004

Five urease-positive Vibrio parahaemolyticus strains were isolated from Kamak Bay in Yeosu in 2002 and 2003. V. parahaemolyticus YKB4 and YKB14 were isolated from seawater, YFB20 from black rockfish (Sebastes schlegeli), and YFO2l and YFO22 from olive flounder (Paralichthys olivaceus). The five urease-positive strains (YKB4, YKB14, YFB20, YFO21, and YFO22) did not show hemolysin and protease activity, while they did alter in color (to red) as the bacteria grew in the urea broth medium. All samples showed identical biochemical characteristics as a reference strain, V. parahaemolyticus KCTC2471, except in urease production. The five urease-positive strains showed urease activities at a mid stationary phase, and their activity was maximal in the late stationary phase of their culture supernatant. The addition of urea to the Luria-Bertani (LB) broth medium significantly affected the initial production of urease of V. parahaemolyticus isolates. Mortality by urease-positive V. parahaemolyticus YKB4, YKB14, YFO2l, and YFO22 was significantly high, being$60-80\%$, while YFB20 only reflected a rate of $20\%$. Protease-positive V. parahaemolyticus FM39 and FM50 showed a $40\%$ and $60\%$ mortality rate, respectively. However, hemolysin-positive V. parahaemolyticus had no mortality, like the non-pathogenic V. parahaemolyticus KCTC2471, while V. vulnificus resulted in a $40\%$ mortality rate. Injection with urease-positive V. parahaemolyticus strains showed mortality within 12 hrs in mice, and the strains could be isolated from the dead mice.

• 한국식품영양학회지
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• 제30권3호
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• pp.599-608
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• 2017

This study investigated the quality characteristics and volatile flavor components of aronia wine (Aronia melanocarpa (0~100%)). After 12 days of fermentation, the alcohol contents of aronia wines ranged between 9.0~12.0%. The pH level and total acidity of aronia wines were 3.20~3.68 and 0.57~0.76 g/100 mL, respectively. The organic acid analysis of wine containing 100% aronia, revealed malic acid content at 3.70 mg/mL, followed by tartaric acid, lactic acid, and citric acid. As the aronia content increased, both the total polyphenol content and the antioxidant activity (the DPPH radical scavenging activity) also significantly increased. The total polyphenol content was the highest in the wine with 100% aronia (461.33 mg%), and the antioxidant activity showed the highest values in the wine with 100% aronia (91.91%). Volatile flavor component analysis of aronia wines identified 8 alcohols, 12 esters, 4 ketones, and 7 other compounds. In the sensory evaluation, the color, flavor, and taste of wine with 20% aronia showed higher values than other aronia wines. Based on the results of the present study, we suggest that 20% aronia is most beneficial in improving the quality as well as sensory characteristics of the wine.

• 한국식품과학회지
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• 제45권2호
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• pp.248-251
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• 2013

김에 존재하는 M. roseus는 비병원성 세균이나 사람이 면역력이 떨어지게되면 질병을 일으킬 수 있는 균주로서 열처리를 통해서도 쉽게 사멸되지 않고 특히 10 kGy의 감마선 조사에서도 1 log, 40 kGy의 조사선량에서 1 log CFU/mL 이상의 생존율을 나타내는 방사선 저항 세균이다. 이러한 방사선 저항 세균인 M. roseus를 광펄스 살균을 통해 살균 가능성을 알아보았다. M. roseus는 빛의 세기가 증가함에 따라 사멸율이 증가하는 경향을 나타냈으며, 1,000 V의 빛의 세기에서 3분 처리 후에 6.4 log의 감소효과를 보였고 tailing 현상을 보이지 않았다. 빛의 세기와 처리시간을 같이 한 조건에서는 단위 시간동안 펄스 수가 높을수록 살균율이 높아졌으며, 10 pps에서는 2분의 처리로 모든 균이 사멸하였다. 또한 광원과 시료사이의 거리는 가까울수록 사멸율이 증가하는 경향을 보였다. 이러한 연구 결과로 볼 때 광펄스 살균이 향후 김의 위생적 처리의 방법의 하나로 가능성이 있을 것으로 예측된다.

• 한국식품조리과학회지
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• 제12권3호
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• pp.361-366
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• 1996

Acceptable fish noodles of better nutritional and sensory values than conventional noodle made only wheat flour were prepared by mixing wheat flour and ground tile fish (Branchioste gus japonicus). In oder to mask a distinctive fish odor, various herbs and spices, such as ginger (Zingiber officinale), curry (Chalcas koenigii), nutmeg (Myristica Pagrans), garlic (Ailium sativum), black pepper (Piper nigrum), lemon (Cirtus limon) or sodachi (Citrus sudachi) were added to the basic tile fish noodle. Also, for the purpose of enhancing eating qual ify and nutritional value of basic noodle were combined some agricultural or fishery products of Cheju island, citrus fruits; danyooja (Citrus danyooga), hagul (C. natfudaidai), medical plants; angelica utilis (Angelica keiskei), ginseng (Panax ginseng), cactus (Opuntia dillenii), vegetable; carrot (Daucus carota), dropwort (Oenanthe jnvanica), seaweeds; fusiforme (Hizkia fusiforme), gulfweed (Sargaceae hlvelium). The optimal mixing ratio for preparing the basic tile fish noodle amounted to ground tile fish 135 g: tile fish stock 139 $m\ell$: wheat flour 450 g: salts 10 g. The mixture of curry powder (2.5 g) and nutmeg powder (2.5 g) was proved to be the most effective combinations for masking unfavorable fish odor. The optimal amounts of materials to be added to the prepared basic noodle were 25 g citrus zest and 80m1 citrus juice for citrus fruits noodles, and ca. 140 g puree for noodles from medical plants, vegetables and seaweeds, respectively. The preference score obtained from consumer preference test, on a 9-point scales, were in oder of i) danyooja > carrot, angelica, ginseng > fusiforme, ii) hagul > dropwort, cactus > gulfweed. The shelf-life of tile fish noodle based on bacterial counting was estimated to be 7-days at 5$^{\circ}C$.

• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.162-167
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• 2009

Reproductive abilities in beef cattle herds are receiving increased attention due to recent rises in production costs. To achieve more efficient management, a measure of fertility, namely the number of calves produced at k yr of age ($NCP_k$), was developed and its genetic parameters were estimated from Japanese Black cows by restricted maximum likelihood procedures. The k examined were distributed from 2 to 10 yr of age and $NCP_2$ averaged 1.077 calves over 43,536 cows. The averages increased by approximately 0.9 calf with each additional 1 yr increment in k. Heritabilities of $NCP_k$ were estimated to be low ranging from 0.083 to 0.162, which seemingly suggested a difficulty of genetic improvement. However, large genetic variation and high accuracy were observed in predicted breeding values of $NCP_k$. For example, the breeding values of $NCP_7$ were predicted between -0.303 and +0.213 with average accuracy of 0.607 for cows with observations. Genetic correlations among different k were generally high and positive (0.474 to 0.995). The analyses showed that at least $NCP_4$ was required to maintain the genetic correlations of 0.8 or higher with subsequent $NCP_k$. Also $NCP_5$ maintained the genetic correlations of 0.9 or higher with subsequent $NCP_k$ The results suggested some possibilities for $NCP_k$ to be a selection criterion considering its genetic variation, high accuracy and consistency with subsequent performance.

• 한국수산과학회지
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• 제42권4호
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• pp.322-328
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• 2009

Three-hundred and sixteen Escherichia coli strains from seawater, and a variety of farmed fishes, including oliver flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major) and sea bass (Lateolabrax japonicus) between May to October in 2004, were tested by agar dilution method to determine their susceptibility patterns to 17 antimicrobial agents. Overall, 92.1% of Escherichia coli isolates from samples showed antimicrobial resistance to at least one antimicrobial agent and the multiple resistance was seen in 173 isolates (54.7%). The resistance of E. coli isolates to tetracycline (74.1%) was highest, followed by cephalothin (69.9%), doxycycline (66.5%), streptomycin (47.2%), ampicillin (46.2%), cefazolin (31.6%), enrofloxacin (31.0%). norfloxacin (28.2%). The most frequent resistance pattern was TE-D-CF-CIP-ENO-NOR-AM-S-C-SXT-AmC-CZ (14.7%), followed by CF (6.2%), TE (5.1%), TE-CF (4.5%) in 177 isolates from fishes and TE-D-CF (7.2%) followed by TE-D-CF-S (5.8%), CF and TE-D-S (3.6%) in 139 isolates from seawater.

• 대한한의학방제학회지
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• 제17권2호
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• pp.133-142
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• 2009

Objective : Recently, obesity has increased at an alarming rate and is now a worldwide health problem. Natural dietary supplement, $CharmSlim^{(R)}$(C), is mixed pills, which consists of beans, black sesames, kelps, onions, adlay seeds, anchovies, pyogo mushrooms, green tea. Our study was performed to determine the weight-loss effects of natural dietary supplement, based on natural ingredients, in mice fed high-fat diet with an abundance of carbohydrates. Methods : Four groups of male ICR mice were fed different diets during 6 weeks: normal diet(NOR), high-fat (15%, w/w) diet(HF), high-fat supplemented with natural dietary supplement powdered 5%(C5) and 10%(C10) groups. We measured the changes of food intake, body weight and adipose tissues weights. Also we examined levels of lipid profiles in serum. Results : Body weight, liver weight and food efficiency ratio were significantly higher in the HF group than in NOR group. After 6 weeks of treatment, body weight, liver weight and adipose tissues weights (epididymal, perinephric, visceral and BAT) were significantly decreased in the C10 group when compared to the control HF group. The C10 group had markedly lower serum levels of total cholesterol and triglyceride when compared with HF group. Conclusion : These results demonstrate that this dietary supplement can suppress the increased lipid profiles, body weight and fat gains, thus could be considered as an effective adjunct in the treatment of obesity.

• 한국식품과학회지
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• 제31권3호
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• pp.581-585
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• 1999

항산화활성이 높은 찰옥수수를 육종하고, 옥수수의 항산화활성에 가장 큰 효과를 나타내는 물질을 추적하고자, 찰옥수수 자식계통과 재래종의 항산화활성을 측정하고 페놀성화합물, tocopherols, carotenoids의 함량을 측정하였다. 전자공여능에 의한 항산화활성은 $15.5{\sim}65.0%$였고, 페놀성화합물은 $102.3{\sim}323.5\;{\mu}g/mL$였으며, tocopherols는 $15.6{\sim}144.2\;{\mu}g/mL$였고, A450의 값은 $0.047{\sim}0.206$였다. 4종의 검정찰옥수수는 항산화활성이 48.7%, 페놀성화합물은 $267.0\;{\mu}g/mL$, tocopherols은 $87.0\;{\mu}g/mL$로 상당히 높았다. 항산화활성과 페놀성화합물, tocopherols의 함량과는 고도의 상관관계가 있었으나 carotenoids와는 유의성이 없었다.

• 한국수산과학회지
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• 제38권6호
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• pp.379-384
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• 2005

A high-performance liquid chromatography assay method for oxolinic acid in fish products was developed, evaluated and validated through the monitoring of oxolinic acid based on farming and distribution. The recovery rate of the developed method was $102.3-106.7\%$ as compared to conventional methods. The stock solution was stable for 3 weeks under refrigerated condition at $4^{\circ}C$ The performance limit was evaluated as 0.01ppm of oxolinic acid in fish muscle. 478 fish samples such as olive flounder, genuine porgy, common sea bass and black rock fish collected from fish farms in the coastal area from September 2001 to October 2004 were analyzed to evaluate overall efficiency of the modified method and to monitor the actual condition of oxolinic acid usage in fish farm. According to the monitoring results, the modified method was suitable for analysis of oxolinic acid in fish muscle and oxolinic acid might be used in a small portion of fish farms. The suggested analysis method of oxolinic acid was registered in the Korean Official Methods of Food Analysis and is being utilized for fishery products by the Korea Food and Drug Adminstration and the National Fisheries Products Quality Inspection Service.

• Journal of Ginseng Research
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• 제13권1호
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• pp.123-129
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• 1989

국내외로 다변화하는 소비기호추세에 영합할 수 있는 제형으로의 제품개발을 위하여 국내의 가정주부를 대상으로 소비기호동향을 조사 $.$ 분석한 결과를 요약하면 다음과 같다. 가정주부들이 애용하는 다류는 커피>율무차>인삼차>홍차의 순이었고, 80% 이상이 인삼을 식품으로 인식하고 있었으며, 56% 정도가 인삼의 효과를 인정하였으나 약 43%가 체질에 따라 다르다고 인식하고 있어 체질과의 상관관계 연구가 필요한 것으로 나타났다. 90% 이상이 인삼의 복용경험을 가지고 있었고, 복용 시 기대효과는 주로 약리적인 것으로 과음이 으뜸이었다. 복용방법은 전통적인 복용형태인 탕으로 다려 먹는 방법을 가장 애용하고 있었다. 인삼의 향취미는 88% 이상이 좋은 반응을 보였고, 70% 이상이 유통가격을 복용의 기피요인으로 평가하고 있어 유통구조개선과 아울러 원가절감 개선을 위한 연구가 절실한 것으로 사료된다. 시판 인삼가공제품에 대한 인지율과 구입 및 복용경험은 제형에 따라 큰 차이가 있는 것으로 나타났다. 인삼차와 인삼드링크류는 기호도가 아주 높은 반면 인삼캡슐과 인삼타브렐는 아주 낮았고, 평균 인지갯수는 4개이었다. 연령에 따라 인삼 및 인삼가공제품에 대한 기호도가 상당한 차이를 나타내고 있어 소비대상을 연령별로 면밀히 분석 구분하고 기호에 영합할 수 있는 새로운 제품으로의 제형개발과 기존제품의 품질개선이 이루어져 야 할 것으로 사료된다.