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http://dx.doi.org/10.5657/kfas.2009.42.4.322

Antimicrobial Susceptibility of Escherichia coli Isolated from Fish Farms on the Southern Coast of Korea  

Son, Kwang-Tae (South Sea Fisheries Research Institute, NFRDI)
Oh, Eun-Gyoung (Food Safety Research Center, NFRDI)
Park, Kun-Ba-Wui (South Sea Fisheries Research Institute, NFRDI)
Kwon, Ji-Young (South Sea Fisheries Research Institute, NFRDI)
Lee, Hee-Jung (Food Safety Research Center, NFRDI)
Lee, Tae-Seek (Aquaculture Environment Institute, NFRDI)
Kim, Ji-Hoe (Aquaculture Environment Institute, NFRDI)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.42, no.4, 2009 , pp. 322-328 More about this Journal
Abstract
Three-hundred and sixteen Escherichia coli strains from seawater, and a variety of farmed fishes, including oliver flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major) and sea bass (Lateolabrax japonicus) between May to October in 2004, were tested by agar dilution method to determine their susceptibility patterns to 17 antimicrobial agents. Overall, 92.1% of Escherichia coli isolates from samples showed antimicrobial resistance to at least one antimicrobial agent and the multiple resistance was seen in 173 isolates (54.7%). The resistance of E. coli isolates to tetracycline (74.1%) was highest, followed by cephalothin (69.9%), doxycycline (66.5%), streptomycin (47.2%), ampicillin (46.2%), cefazolin (31.6%), enrofloxacin (31.0%). norfloxacin (28.2%). The most frequent resistance pattern was TE-D-CF-CIP-ENO-NOR-AM-S-C-SXT-AmC-CZ (14.7%), followed by CF (6.2%), TE (5.1%), TE-CF (4.5%) in 177 isolates from fishes and TE-D-CF (7.2%) followed by TE-D-CF-S (5.8%), CF and TE-D-S (3.6%) in 139 isolates from seawater.
Keywords
Escherichia coli; Antimicrobial susceptibility; Farmed fish; Seawater;
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1 Acar, J,F. and F.W, Goldstein. 1991. Disk susceptibility test. In: Antibiotics in Laboratory Medicine, Lorian, V. Ed., Williams & Wilkins, Baltimore, 17-52
2 Von Baum, H. and R. Marre. 2005, Antimicrobial resistance of Escherichia coli and therapeutic implications. Int. J. Medic. Microbiol., 295, 503- 511   DOI   ScienceOn
3 Cho, J.K. J.S. Ha and K.S. Kim, 2006. Antimicrobial drug resistance of Escherichia coli isolated from cattle, swine and chicken, Kor.J. Vet. Publ. Hlth., 30, 9-18
4 Chio, S.H., YJ. Lee, B.H. Kim, KS. Kim, C.K. Park, D.H. Bae, J.K. Cho, J,W, Kim, RH. Kim and M.S, Kang. 2006. Antibiotic resistance and genotypic characterization of Escherichia coli isolated from pig farm environment. J, Bacteriol. Virol., 36, 159-165   DOI   ScienceOn
5 Fey, P,D., T.J, Safranek, M.E, Rupp, E.F. Dunne, E. Ribot, p,c. Iwen, P.A. Bradford, F.J. Angulo and S. Hinrichs. 2000. Ceftriaxone-resistant salmonella infection acquired by a child from cattle. N, Engl. J. Med., 27, 1242-1249   DOI   ScienceOn
6 Heo, J.H, M.H. Jung, M.H. Cho, G.H. Kim, K.C. Lee, J.H. Kim and T.S, Jung. 2002, The study on fish disease with reference to bacterial susceptibility to antibiotics in the southern area of Kyeongnam. J. Vet. Clin., 19, 19-22
7 Lee, J.I., G.Y. Han and H.H. Park. 2003. Characteristics and antibiotics susceptibility of Escherichia coli isolated from fIshery products. Korean J. Food & Nutr. 16, 111-115
8 Levin, B.R., M. Lipsitch, V. Perrot, S. Schrag, R. Antia, L. Simonsen, N.M. Walker and F.M. Stewart. 1997. The population genetics of antibiotic resistance. Clin. Infect Dis., 24, Suppll, S9-16   DOI
9 Lim, S.K., H.S. Lee, J.R. Byun, S.Y. Park and S.C. Jung. 2007b. Antimicrobial resistance of commensal bacteria isolated from food-producing animals. I. Antimicrobial resistance of Escherichia coli and EnteroCOCClls spp. isolated from pig faecal samples. Kor. J. Vet. Publ. Hlth., 31, 31- 39
10 Sung, M.S, J.H. Kim, J.S. Ha, J.K. Cho, S.Y. Seol and K.S. Kim. 2008. Biochemical properties and serotypes of pathogenic Escherichia coli isolated from poultry in Korca. Kor. J. Vet. Res., 48, 145- 151
11 Tollefson, L., S.F. Altekruse and M.E. Potter. 1997. Therapeutic antibiotics in animal feeds and antibiotic resistance. Rev. Sci. Tech. 16, 709-715   DOI   PUBMED   ScienceOn
12 You, J.Y., B.M. Moon, J.G. Oh, B.K Baek, L.G. Li, B.S. Kim, B.D. Stein and 1.R. Lee. 2006. Antimicrobial resistance of Escherichia coli 0157 trom cattle in Korea.. Int. J. Food Microbiol., 106, 74- 78   DOI   ScienceOn
13 Son, K.T., E.G. Oh, T.S. Lee, H.J. Lee, P.H. Kim and J.H. Kim. 2005. Antimicrobial susceptibility of Vibrio parahaemolyticus and Vibrio alginolyticus from fish farms on the southern coast of Korea. J. Kor. Fish. Soc., 38, 365-371
14 Kim, A.R., Y.M. Cho, S.K. Lim, M. Her, W.S. Jeong, S.C. Jung and J.H. Kown. 2007. Antimicrobial resistance of commensal bacteria isolated from food-producing animals. I. Antimicrobial resistance of Escherichia coli and Enterococcus spp, isolated from chicken faecal samples, Kor. J. Vet. Publ. Hlth., 31, 41-49   ScienceOn
15 A.P.H.A. 1970, Recommended Procedures for the Examination of Sea Water and Shellfish. 4th ed., American Public Health Association, Washington, D,C., 105 pp
16 Marano, N.N., S. Rossiter, K Stamey, K. Joyce, T.J. Barrett, L.K Tolldfson and F.J. Angulo. 2000. The national antimicrobial resistance monitoring system (NARMS) for enteric bacteria, 1996-1999: surveillance for action. J. Am. Vet. Med. Associ., 217, 1829-1830
17 KFDA (Korean Food and Drug Administration), 2007. Monitoring of antimicrobial resistance on the food animals and meats. Seoul, 1-110
18 Gaskins, H.R., C.T. Collier and D,B. Anderson. 2002. Antibiotics as growth promotants: mode of action. Anim. Biothechnol., 13, 29-42   DOI   ScienceOn
19 Tendencia, E.A. and L.D. Pena. 2001. Antibiotic resistance of bacteria from shrimp ponds. Aquaculture, 195, 193-204   DOI   ScienceOn
20 Kruse, H. and H. Sorum. 1994. Transfer of multiple drug resistance plasmids between bacteria of diverse origins in natural microenvironemnts. Appl. & Environ. Microbiol., 60, 4015-4021   PUBMED
21 NCCLS. 2004. Performance standards for antimicrobial susccptibility tcsting. NCCLS document MlOO-S14. National Committee for Clinical Laboratory Standards, Wayne, PA
22 Frieden, T.R., S.S. Munsiff, D.E. Low, B.M. Willey, G, Williams, Y. Faur, W. Eisner, S. Warren and B. Kreiswirth. 1993. Emergence of vancomycin resistant enterococci in New York City, Lancet, 342, 76-79   DOI   ScienceOn
23 Beam, J.W. and B. Buckley. 2006. Community acquired methicillin resistant Staphylococcus aureus: Prevalence and risk factors. J. Athl. Train., 41, 337- 340   PUBMED
24 Lau, M.M. and S.C. Ingham, 2001. Survival of faecal indicator bacteria in bovine manure incorporated into soil. Lett, Appl. Microbiol., 33, 131-136   DOI   ScienceOn
25 Aaestrup, F.M., F. Bager, N.E. Jesen, M. Madsen, A. Meyling and H.C. Wegener. 1998. Surveillance of antimicrobial resistance in bacteria isolated from food animals to antimicrobial growth promoters and related therapeutic agents in Denmark. APMIS, 106, 606-622   PUBMED
26 Herwig, R.P., J.P. Gray and D.P. Weston. 1997. Antibacterial resistant bacteria in surficial sediments near salmon net-cage farms in Puget Sound, Washington, Aquaculture, 149, 263-283   DOI   ScienceOn
27 Kummerer, K 2009, Antibiotics in the aquatic environment: a review-part I . Chemosphere, 417-434   DOI   PUBMED
28 Lee, H., Y.H. Oh, S,G, Park and S,M. Choi. 2007. Antibiotic susceptibility and distribution of Vibrio parahaemolyticus isolated from the seafood, Kor. J. Env, Hlth., 33, 16-20
29 Longlois, B.E., K.A. Dawson, I. Leak and D.K Aaron. 1988. Antimicrobial resistance of fecal colifonns from pigs in a herd not exposed to antimicrobial agents for 126 months. Vet. Microbiol., 18, 147- 153
30 Lim, S.K., H.S. Lee, J.R. Byun, S.Y. Park and S.C. Jung. 2007a. Antimicrobial resistance of commensal bacteria isolated from food-producing animals. I. Antimicrobial resistance of Escherichia coli and Enterococcus spp. isolated from cattle faecal samples. Kor. J. Vet. Publ. Hlth., 31, 21- 29
31 Wise, R. 2002. Antimicrobial resistance: priorities for action. J. Antimicrob. Chemoth., 49, 585-586   DOI   ScienceOn
32 Kummerer, K and A. Henninger. 2003. Promoting resistance by the emission of antibiotics from hospitals and households into effluents, Clin, Microbiol. Infec., 9, 1203-1214   DOI   ScienceOn